Labshake search
Citations for PNA Bio :
1 - 50 of 115 citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2019Quote: ... 0.375 μl mixed rRNA gene-blocking peptide nucleic acids (PNAs; 1:1 mix of 100 μM plastid PNA and 100 μM mitochondrial PNA; PNA Bio), 0.5 μl Kapa dNTPs ...
-
bioRxiv - Neuroscience 2020Quote: ... Cas9 protein was ordered from PNA Bio (CP01-50, Thousand Oaks, CA) and used to check cutting efficiency of the guides in vitro ...
-
bioRxiv - Neuroscience 2021Quote: ... fertilized eggs were injected with precomplexed RNP (ribonucleoprotein)-Cas9 protein (PNA Bio, 50 ng/μl) and sgRNA (30 ng/μl) ...
-
bioRxiv - Plant Biology 2022Quote: ... PCR reactions for plant samples were supplemented with a custom peptide nucleic acid (PNA) blocker (ITS1PNABlk: 50-O-E–E-GTCGTGTGGATTAAA-30; PNA BIO INC, Thousand Oaks, CA) to block host plant ITS sequences ...
-
bioRxiv - Cell Biology 2022Quote: ... 80 pg of sgRNA was co-injected with 200 pg of Cas9 protein (#CP01-50; PNA Bio, Newbury Park, CA) into 1-cell zebrafish embryos ...
-
bioRxiv - Genetics 2020Quote: ... An injection mix was made with 40 ng/ul sgRNA RNA and 300 ng/ul Cas9 protein (PNA Bio # CP01-50) and injected into embryos from our BDC stock (Fig ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cas9 (PNA Bio, CP01-50) ribonucleic particles (CRNPs ...
-
bioRxiv - Plant Biology 2021Quote: ... and 1 μM blocking primers (mPNA and pPNA, PNA BIO, Inc., Newbury Park, CA). PCR was performed using the following specifications ...
-
bioRxiv - Plant Biology 2019Quote: ... and the addition of a Peptide Nucleic Acid (PNA) blocker (PNA Bio, Newbury Park, United States) at a concentration of 0.5 μM/reaction to inhibit plastidial amplification ...
-
bioRxiv - Cancer Biology 2023Quote: ... and incubated with a commercially available Cy3-labeled (CCCTAA)3 peptide nucleic acid probe (PNA Bio) recognizing mammalian telomere sequences ...
-
bioRxiv - Immunology 2020Quote: ... Cas9 protein (PNA bio) and gRNA were incubated at room temperature for 15 min in a 3:1 reaction with 15μg Cas9 and 5000 ng gRNA (2500ng of sgRNA#1 and 2500ng of sgRNA#2).47 The incubation product (gRNA and Cas9 complex ...
-
bioRxiv - Developmental Biology 2021Quote: ... Recombinant Cas9 protein (PNA Bio) and sgRNAs were injected at concentrations of 400 ng/µl of Cas9 protein and 100 ng/µl for each sgRNA ...
-
bioRxiv - Genetics 2021Quote: Cas9 protein (PNA Bio, Inc.) was mixed to a final concentration of 700 ng/ul with sgRNA (37.5 ng/ul ...
-
bioRxiv - Genetics 2022Quote: ... 750ng Cas9 protein (PNA Bio) and 375ng integration site gRNA were mixed ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Cas9 protein (PNA Bio #CP01), phenol red ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cas9 protein (PNA Bio CP02) at 50ng/µL ...
-
bioRxiv - Genetics 2023Quote: ... and Cas9 protein (PNA bio). A single gRNA with validated cleavage activity was chosen for incorporation into a synthetic gBlock (IDT ...
-
bioRxiv - Genetics 2022Quote: ... Cas9-NLS protein (PNA Bio) was added to the gRNA and incubated at room temperature for 5 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... RNA-protein complexes (RNPs) were prepared by mixing Cas9 protein 30-60 ug (PNA Bio) and 15-30 ug of gRNA per aliquot followed by incubation of 10 minutes at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... complexed with Cas9 protein (PNA Bio), and injected into 1-cell stage embryos ...
-
bioRxiv - Developmental Biology 2020Quote: ... the protein Cas9 (recombinant cas protein from S. pyogenes PNA Bio CP01, final concentration 100 ng/μL) and KCL (final concentration 200 mM) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and then subjected to FISH using a peptide nucleic acid (PNA) probe specific to the telomeric sequence (CCCTAA)3 (TelC-Cy3, PNA BIO). Cells were dehydrated successively in 70% ...
-
bioRxiv - Cancer Biology 2020Quote: ... Hybridization with 333ng/mL of a TelC-Cy5-labeled peptide nucleic acid (PNA) oligonucleotide telomere probe (N-CCTAACCTAACCTAA-C, PNA BIO) in PNA buffer (10mM Tris pH 7.5 ...
-
bioRxiv - Bioengineering 2020Quote: Anticoagulant activity of 2HF-2NN1 was reversed using ssDNA purchased from IDT and ssPNA (peptide-nucleic-acid) antidote strands purchased from PNA Bio. A solution containing two specific antidotes (500 μM ...
-
bioRxiv - Genetics 2020Quote: ... lyophilized Cas9 protein (PNA Bio Inc, CP01) was reconstituted to a stock concentration of 1 μg/μl in 20 mM Hepes ...
-
bioRxiv - Bioengineering 2020Quote: ... purified SpyCas9 protein 30 pmol (PNA Bio, #CP02 or 3xNLS-SpCas943(prepared by the Scot Wolfe laboratory ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cas9 protein was purchased from PNA Bio Inc ...
-
bioRxiv - Neuroscience 2021Quote: ... purified recombinant Cas9 protein (PNA Bio, 300ng/ul) and donor plasmid (500ng/ul ...
-
bioRxiv - Developmental Biology 2021Quote: ... sgRNA was mixed with Cas9 Protein (PNA Bio) and Texas Red Dextran ...
-
bioRxiv - Genomics 2019Quote: ... 10 ug Cas9 protein (PNA Bio CP01-20) was mixed with 10 ug modified guideRNA (Synthego ...
-
bioRxiv - Developmental Biology 2022Quote: ... and CAS9 protein (500 ng/µL, PNA Bio) in HEPES buffer pH 7.5 (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cas9 protein with NLS (PNA Bio, CP01-200) was resuspended in 20% glycerol/water to a concentration of 1mg/ml ...
-
bioRxiv - Bioengineering 2023Quote: ... The Cas9 protein powder was purchased from PNA BIO Inc ...
-
bioRxiv - Genetics 2023Quote: ... The Cas9 protein was purchased from PNA Bio (CP01). All homozygous animals edited by CRISPR/Cas9 were validated by Sanger sequencing ...
-
bioRxiv - Developmental Biology 2024Quote: ... 2400 ng Cas9 protein with NLS (PNA Bio CP01) were combined with 1200 ng of sgRNA in 4.5 μl nuclease-free water on the day of injection and left at room temperature for 10 minutes to form ribonucleoprotein complexes ...
-
bioRxiv - Developmental Biology 2019Quote: ... 200 pg/nl Cas9 protein (PNA Bio, Newbury Park, CA), or a combination of both reagents ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... sgRNAs were mixed with purified Cas9 protein (PNA Bio #CP01) with a final injected concentration of 0.05% phenol red to visualize the injection mix ...
-
bioRxiv - Bioengineering 2020Quote: ... 150ng of Cas9 protein (PNA Bio, Inc., Newbury Park, CA), 1μL of 10X BSA ...
-
bioRxiv - Developmental Biology 2023Quote: ... but later studies utilized Cas9 protein (CP01, PNA BIO INC). When Cas9 protein was used we incubated the Cas9 protein/sgRNA mixture at 37°C for 5 minutes before injection ...
-
bioRxiv - Neuroscience 2023Quote: ... 60 pg sgRNA and 100 pg Cas9 protein (PNA Bio) were co-injected into one-cell stage wild-type embryos ...
-
bioRxiv - Neuroscience 2024Quote: ... 25ng/μL gRNA1 and 50ng/μL SpCas9 protein (PNA Bio) was microinjected into the pronucleus of zygotes positive for 3’ LoxP site ...
-
bioRxiv - Neuroscience 2024Quote: ... 25ng/μL gRNA2 and 50ng/uL SpCas9 protein (PNA Bio) was microinjected into the pronucleus of C57BL/6 zygotes ...
-
bioRxiv - Developmental Biology 2021Quote: ... Recombinant Cas9 protein (900 ng μl−1; PNA Bio, #CP01-20) was co-injected with both sgRNAs (20μM each ...
-
bioRxiv - Cell Biology 2022Quote: ... Recombinant Cas9 protein containing a nuclear localization signal (PNA Bio Inc) was reconstituted to a solution of 1 mg/mL Cas9 protein in 20 mM HEPES ...
-
bioRxiv - Bioengineering 2020Quote: ... 167ng/μL of Cas9 protein (PNA Bio, Inc., Newbury Park, CA) and 133ng/μL of donor plasmid ...
-
bioRxiv - Developmental Biology 2022Quote: ... one dorsal blastomere was injected with 1ng Cas9 protein (PNA Bio), 250pg shroom3-targeted sgRNA (target sequence GUAGCCGGAGAGAUCACUUG ...
-
bioRxiv - Developmental Biology 2021Quote: ... one dorsal blastomere was injected with 1ng Cas9 protein (PNA Bio), 250pg shroom3-targeted sgRNA (Synthego)(Methods Appendix Fig ...
-
bioRxiv - Genetics 2019Quote: ... Purified Cas9 protein (400 ng/μl) (PNA Bio, Thousand Oaks, CA) and sgRNAs (100 ng/μl ...
-
bioRxiv - Bioengineering 2019Quote: ... Synthetic gRNAs (Synthego) and recombinant Streptococcus pyogenes Cas9 protein (PNA Bio Inc.) were obtained commercially and diluted to 1,000 ng/µL in nuclease-free water and stored in aliquots at –80°C ...
-
bioRxiv - Neuroscience 2020Quote: ... before adding recombinant Cas9 protein (300 ng/µL; PNA Bio, CP01-200) for embryo injection.