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Citations for Sartorius :
1 - 50 of 60 citations for Strong Cation Exchange Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The TFF retentate was next enriched for AAV particles on a cation exchange chromatography column (BIA Separations, Sartorius). The eluate was concentrated ...
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bioRxiv - Molecular Biology 2022Quote: ... Buffer exchange was accomplished using Vivaspin concentrators (Sartorius). Protein purity was assessed by SDS-PAGE followed by Coomassie blue staining ...
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bioRxiv - Microbiology 2023Quote: ... and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Microbiology 2024Quote: ... and buffer exchange to PBS using VivaSpin columns (Sartorius) was carried out ...
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bioRxiv - Biochemistry 2020Quote: ... Imidazole was removed by buffer exchange using a Vivaspin centrifugal filter (Sartorius) with a 10 kDa cut-off ...
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bioRxiv - Immunology 2022Quote: ... A CIMac pDNA 0.3 mL weak anion-exchange analytical column (Sartorius, Germany) was used with a Shimadzu
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bioRxiv - Immunology 2020Quote: ... Purified viral particles were obtained by Ion Exchange (VivaPure® AdenoPACKTM 20, Sartorius), and the DNA extracted and subjected to Next Generation Sequencing ...
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bioRxiv - Microbiology 2023Quote: ... After two buffer exchanges with trypsin buffer on Vivaspin 15R (5 kDa) columns (Sartorius), the samples were digested by trypsin with 1/80 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... followed by concentrating and buffer exchange into IMAC binding buffer using Vivaflow 100 system (Sartorius). The resulting protein solution was purified by Histrap column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... Buffer exchange and protein sample concentration were performed using 10 kDa MWCO Vivaspin Turbo centricons (Sartorius, Germany). After IMAC ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Neuroscience 2022Quote: ... Buffer exchange and concentration was done with Vivaspin 20 ultrafiltration unit having a 1.000.000 molecular weight cut-off (Sartorius). Viral particles were washed and resuspended in PBS and titered using quantitative PCR with primers targeting WPRE element.
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated toxins were purified using buffer exchange columns (Vivacon 500, Sartorius, 3000 Da Molecular Weight Cut-Off) using the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Virus was partially purified using depth filtration and anion exchange chromatography with syringes and small-scale filters (C0SP 23 cm2 [Merck] and Sartobind Q Nano [Sartorius]). Buffer compositions and other parameters were as previously described but scaled proportionately to the product and filter sizes (5) ...
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bioRxiv - Biochemistry 2020Quote: ... 5% ethylene glycol), followed by buffer exchange to (30 mM Tris/HCl, pH 8.0) with Vivaspin centrifugal concentrator (Sartorius, Germany). The final phosphoprotein concentrations were adjusted to 1.5 mg/ml (80 μM) ...
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bioRxiv - Biophysics 2020Quote: ... MgCl2 and EGTA were removed by 4 times buffer exchange using a Vivaspin 500.5 molecular weight cut-off (Sartorius, Göttingen). Samples were loaded onto a SDS-PAGE gel to confirm phosphorylation.
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bioRxiv - Cell Biology 2024Quote: ... Buffer exchange from iodixanol to Ringer’s lactate buffer was then carried out using Vivaspin 20 columns (Sartorius, VS2042, Göttingen, Germany). To obtain high virus titers ...
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bioRxiv - Cell Biology 2022Quote: ... GCP6-tagged purified γ-TuRC was then subjected to buffer exchange using Vivaspin 500 centrifugal concentrator (10 kDa MWCO, Sartorius VS0102) for a final NaCl concentration of 150 mM in eluate ...
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bioRxiv - Synthetic Biology 2022Quote: ... pure Q-GRFT was prepared by flow-through membrane chromatography on a salt-tolerant anion exchange capsule (Sartobind STIC PA nano, Sartorius, Goettingen, Germany) according to manufacturer instructions.
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bioRxiv - Plant Biology 2023Quote: ... Finally, protein concentration and buffer exchange (20 mM Tris-HCl, pH 7.5) was performed using Vivaspin 30K columns (Sartorius, Germany, Göttingen, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... the media was changed to 90 µL of growth media containing 1:1000 Incucyte Nuclight Rapid Red Dye (Sartorius, 4717) and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium ...
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bioRxiv - Cancer Biology 2024Quote: ... On the day of the assay tumour media was replaced with fresh media with Incucyte® Cytotox Green Dye (Sartorius, 4633), according to the manufacturer instructions and incubated for 10 min prior T cells addition ...
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bioRxiv - Bioengineering 2022Quote: ... and with two feed media (FMA and FMB -Sartorius). Cell growth (VCC and viability ...
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bioRxiv - Cancer Biology 2023Quote: ... Culture media contained IncucyteⓇ Cytotox Green Reagent (Sartorius, Germany) and IncucyteⓇ Annexin V Red Dye (Sartorius ...
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bioRxiv - Synthetic Biology 2023Quote: ... then viral media was filtered (0.45μM or 0.22μM, Sartorius) before the target cell population was transduced at a MOI < 1 ...
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bioRxiv - Biochemistry 2021Quote: ... The media were then concentrated using a Hydrosart Ultrafilter (Sartorius) and transferred into binding buffer containing 25 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2020Quote: ... Collected media was filtered using 0.45μm PES membrane (Minisart, Sartorius, Germany) to remove extracellular debris ...
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bioRxiv - Bioengineering 2022Quote: ... All experiments were carried out using the same chemically defined media (Sartorius) and Stock Culture Medium (SCM ...
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bioRxiv - Immunology 2022Quote: ... The conditioned media were harvested and concentrated using a Hydrosart ultrafilter (Sartorius), and exchanged into the binding buffer (25 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... The conditioned media were harvested and concentrated using a Hydrosart ultrafilter (Sartorius), and exchanged into the binding buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... the media was refreshed with the addition of 250nM Cytotox green (Sartorius). Pyroptosis was induced with 10μM nigericin (Cayman Chemical ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell media containing lentiviral particles were concentrated using centricon column (Vivaspin 20, Sartorius). Each viral production was titrated beforehand to establish a multiplicity of infection (MOI ...
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bioRxiv - Developmental Biology 2023Quote: ... This media was used in combination with trypsin (0.25%, Sartorius, 03-052-1A) and collagenase (1 mg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... The plate was routinely media changed and imaged daily with Incucyte (Incucyte ZOOM - Sartorius) using a Dual Color 10X 1.22um/pixel Nikon Air Objective (Sartorius cat no 4464) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5 μM Caspase 3/7 dye was included in the media (Sartorius #4704). The number of red nuclei (indicating active caspase 3/7 ...
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Pathway metabolite ratios reveal distinctive glutamine metabolism in a subset of proliferating cellsbioRxiv - Cancer Biology 2024Quote: ... and media replenished at 24 hours for cell confluency measured using IncuCyte-SX5 (Sartorius).
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bioRxiv - Cell Biology 2022Quote: ... Protein was concentrated from the media using VivaSpin Turbo 4 (10000 MWCO) concentrator tubes (Sartorius, VS04T01). Protein samples were run on 4-15% Mini protean TGX stain-free gels (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... Mixed media were subsequently concentrated through spin columns with a cutoff of 3 kDa (Vivaspin, Sartorius). Mixed proteins were separated on a 4-20% Mini-PROTEAN TGX precast gel (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... The aerosols passing through the AF-Fe media were then trapped using gelatine membranes (Sartorius, Germany). The membranes are water-soluble and designed to trap virus-loaded aerosols while retaining their infectivity ...
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bioRxiv - Neuroscience 2020Quote: ... after which conditioned media was collected and concentrated 50-fold using 10 kDa cutoff concentrators (Sartorius #14558502). Protein concentration was measured using the Bradford assay ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then seeded in 96-well plates in cell growth media containing Annexin V Dye (Sartorius). Cells were imaged by real-time fluorescence microscopy (IncuCyte ...
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bioRxiv - Immunology 2022Quote: ... The media was concentrated to 500μL using the Vivaspin 15R Hydrasart 30,000 MWCO columns (Cat. No. FIL8452, Sartorius). This 500μL was loaded onto qEV Original (70nm pore ...
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bioRxiv - Cancer Biology 2023Quote: ... media was changed and live-cell timelapse microscopy of treated cells was performed in an Incucyte SX5 (Sartorius). Images were taken every 30 minutes in the phase ...
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bioRxiv - Cell Biology 2024Quote: ... Large volumes of EV containing media was concentrated by using tangential cross flow ultrafiltration (100 kDa MWCO, Sartorius). EVs were collected in a refrigerated ultracentrifuge at 100,000-110,000 xg for 90 min (Optima XPN-100 ultracentrifuge using a SW28 or SW32Ti rotor ...
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bioRxiv - Biophysics 2022Quote: ... coli strain MG059e16 which harbors a His10>-tag after Gly-83 in the hycE gene of the hycABCDEFGHI operon was expressed in LB media supplemented with 0.4% glucose by anaerobic fermentation in a 200 L reactor (Sartorius). The culture was inoculated with 400 mL of aerobically grown preculture and stirred at 50 rpm for 16 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Then pre-cleared media was concentrated using Vivaflow® 50R cassette with 100 kDa molecular cut-off (VF05H4, Sartorius). Concentrated media was centrifugated at 100000×g for 4 h ...
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bioRxiv - Immunology 2021Quote: ... the thawed media was filtered and concentrated to 150-200 mL by tangential flow with a Vivaflow® 200 (Sartorius). The resulting sample was diluted with an equal volume of Buffer A ...
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bioRxiv - Systems Biology 2020Quote: 2 mL of collected spent media was loaded in Vivaspin 6™ Centrifugal Concentrator 10,000 MWCO PES (Sartorius AG, Germany) and centrifuged at 4000 RPM for 13 minutes to concentrate samples ...
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bioRxiv - Immunology 2022Quote: ... complete media was then layered on top for imaging with an Incucyte S3 live-cell analysis system (Sartorius, Göttingen, Germany). Imaging was performed every hour at 4X magnification using red ...
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bioRxiv - Molecular Biology 2022Quote: ... Conditioned media from hyperflasks was collected and concentrated in bulk using the Sartorius Vivaflow Cross-flow System (Sartorius, NY, USA) with Vivaflow 200 10,000 MWCO PES filters (Sartorius ...