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Citations for Sartorius :
1 - 50 of 77 citations for Siglec 15 Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2022Quote: ... or HIS 1K biosensors (Sartorius/ForteBio) (CrossMAb ...
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bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
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bioRxiv - Bioengineering 2022Quote: ... and was captured by anti-Penta-His (HIS1K) sensors (Sartorius, 18-5120). The primary antibody was diluted to 150nM with a running buffer in wells ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: His-tagged FcαRI (Sinobiological, Cat: 10414-H08H) were immobilized via Ni-NTA biosensor (Sartorius, Cat: 18-5101) and subject to free floating IgA variants to obtained ka ...
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bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... pH 6.0) using a centrifugal concentrator (Vivaspin Turbo 15, Sartorius) and concentrated to a final concentration of 1 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... His-PKR was further concentrated to at least 10 mg/ml using Vivaspin centrifugal concentrator 30 K (Sartorius), snap-frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Biophysics 2021Quote: ... concentrated using Vivaspin Turbo 15 30-kDa centrifugal filter units (Sartorius), and stored at 4°C until use ...
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bioRxiv - Microbiology 2020Quote: ... The purified Rel was concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 50 kDa) ...
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bioRxiv - Microbiology 2023Quote: ... The purified proteins were concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 5 or 50 kDa) ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg/ml in Kinetics buffer (PBS 0.01% BSA) and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biophysics 2024Quote: ... The 50 ml containing 6-His-AdhE was concentrated to a final volume of 10.5 ml using a Vivaspin 20 centrifugal concentrator (Sartorius) with a molecular weight cut-off of 30 kDa and then dialysed against buffer A ...
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
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bioRxiv - Genomics 2020Quote: ... DNA extracts were concentrated to 250 μl using Vivaspin® Turbo 15 (Sartorius) concentrators and purified in large volume columns (High Pure Viral Nucleic Acid Large Volume Kit ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The formulations were sterile filtered (using 0.2 µm filters (Minisart SRP 15, Sartorius)) and administered at 1.2 mg of ivermectin/kg of body mass (i.e ...
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bioRxiv - Bioengineering 2023Quote: The 15 mL fully automated small-scale ambr15® bioreactor (Sartorius, Göttingen, Germany) was used for 14 days production run with 12 mL working volume under two proprietary different feed media supplements ...
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bioRxiv - Biochemistry 2022Quote: ... IgGs produced from the scFvs from the above sort were assessed for their competition of binding with one another using anti-Penta HIS (His1K) biosensors (Sartorius/ForteBio). His1K tips were pre-quenched with buffer containing 10nM biotin ...
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bioRxiv - Biochemistry 2021Quote: ... ultrafiltration was performed using Vivaspin 15 and Vivaspin 2 Hydrosart devices (Sartorius Stedim Biotech) of 2-kDa molecular-mass cutoff ...
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bioRxiv - Plant Biology 2020Quote: ... pH 7.5) was used for gel filtration and Vivaspin 15 with 5 MWCO (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2023Quote: ... Relevant fractions were identified by SDS-PAGE and concentrated using Vivaspin turbo 15 (Sartorius) prior to storage as described above.
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bioRxiv - Physiology 2020Quote: Groups of 15 Drosophila were frozen on dry ice and weighed using a microbalance (Sartorius), then stored at −80°C for glucose analysis ...
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bioRxiv - Evolutionary Biology 2020Quote: The DNA solution was concentrated to 250 μl (Vivaspin Turbo 15, 30,000 MWCO PES, Sartorius) and purified in large volume columns (High Pure Viral Nucleic Acid Large Volume Kit ...
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bioRxiv - Microbiology 2019Quote: ... to an aerated cooling bioreactor set at 15 °C (Sartorius Stedim Biostat Q Plus, Germany). On the other side ...
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bioRxiv - Biochemistry 2024Quote: ... and concentrated over a 3 kDa MWCO membrane using a Vivaspin 15 centrifugal device (Sartorius). The final Ws cytochrome c552 fraction was buffer exchanged to 5 mM Na-phosphate pH 7.0 using a desalting PD-10 column (Cytiva) ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions of interest were pooled and concentrated on a 15 kDa MWCO spin concentrator (Sartorius, #VS2001) and were then loaded onto a Superdex 75 gel filtration column (GE Healthcare ...
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bioRxiv - Bioengineering 2019Quote: ... containing between 260-280 mL medium or in an Ambr 15 bioreactor system (Sartorius, Göttingen, Germany) containing 14.4 mL medium ...
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bioRxiv - Cancer Biology 2020Quote: Multivariate analysis of NMR data was performed using SIMCA 15 software (Sartorius Stedim Biotech, Göttingen, Germany). Principal component analysis (PCA ...
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bioRxiv - Microbiology 2021Quote: ... pooled and concentrated to ∼15-20 mg/ml using Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Small aliquots were flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Microbiology 2023Quote: ... The tag-free RnpM protein was collected and concentrated using a Vivaspin turbo 15 (Sartorius, Germany).
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bioRxiv - Biophysics 2021Quote: ... The eluate from this step was concentrated to 10-15 mL with a Vivaspin® concentrator (Sartorius). Concentrated sample was then exchanged into IEX W buffer (same as IMAC W ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing A2M tetramers were combined and concentrated to 1 ml final volume (Vivaspin Turbo 15, Sartorius).
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bioRxiv - Immunology 2022Quote: ... the supernatant was applied to 2 kDa centrifugal concentrators (Vivaspin 15 R Hydrosart filter device, Sartorius, Germany) and centrifuged for 1 h at 3000 x g according to the suppliers’ protocol ...
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bioRxiv - Bioengineering 2023Quote: ... The serum-free suspended cells were cultured in microbioreactor (Ambr® 15 cell culture bioreactor; Sartorius, Germany) to determine the specific productivity (Qp ...
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bioRxiv - Biophysics 2022Quote: ... freshly prepared FUS was dialyzed against the desired buffer and subsequently concentrated using Vivaspin 15 Ultrafiltration units (Sartorius). Protein purity was confirmed by SDS-PAGE and 1H-15N TROSY/HSQC spectra ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysate was concentrated to 1.5 mL in a 30,000 MWCO concentrator tube (Sartorius Vivaspin Turbo 15 #VS15T21) using the Fiberlite F13B rotor at 2,500 rpm over several 5−10 minute cycles ...
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bioRxiv - Bioengineering 2022Quote: ... for ultracentrifugation and Amicon Ultra-15 isolations and 0.8 μM surfactant-free cellulose acetate syringe filter (16592, Sartorius) for exoEasy isolations ...
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bioRxiv - Biochemistry 2023Quote: ... Obtained EHEP was then concentrated to 15–25 mg/mL using Vivaspin-4 10K columns (Sartorius, Göttingen, Germany). About akuBGL ...
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bioRxiv - Molecular Biology 2021Quote: ... The desalted fraction of SpyCas9 protein was concentrated by using Vivaspin turbo 15 concentrators (50,000 MWCO; Sartorius, Goettingen, Germany). The concentrated protein was quantified by Bradford assay and was kept at −80 °C.
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bioRxiv - Neuroscience 2021Quote: ... Cells were imaged at 15 DIV for 24 hours using the IncuCyte S3 system (Essen Bioscience Sartorius Cat#4763). Using the IncuCyte S3 2019A software (Essen Bioscience Sartorius ...