Labshake search
Citations for Sartorius :
1 - 50 of 144 citations for SARS CoV 2 Spike Glycoprotein S1 RBD Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
-
bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
-
bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
-
bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 5 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
-
bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 S1 RBD was buffer-exchanged (prior to spectral analysis) using a 10 kDa Vivaspin centrifugal filter (Sartorius, Germany) at 12,000 g ...
-
bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
-
bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
-
bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
-
bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
-
bioRxiv - Immunology 2024Quote: The binding kinetics of ZBP1-Zα1Zα2 with SARS-CoV-2 RNAs were evaluated using the Octet RED96 instrument (Sartorius). First ...
-
bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
-
bioRxiv - Immunology 2020Quote: ... and S309 for binding to soluble SARS-CoV-2 S trimer was assessed using the ForteBio Octet HTX (Sartorius Bioanalytical Instruments). All reagents were diluted to 100 nM in PBSF ...
-
bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...
-
bioRxiv - Immunology 2022Quote: Binding kinetics of WS6 IgG and related S2-stem helix antibodies to diverse CoV 2P-stabilized spike proteins (S2Ps) were measured by biolayer interferometry using an Octet HTX instrument (Sartorius). All assays were performed in tilted 384-well plates (Geiger Bio-one ...
-
bioRxiv - Bioengineering 2022Quote: CHO DG44 cell line (Sartorius) expressing a monoclonal antibody (mAb ...
-
bioRxiv - Immunology 2023Quote: ... Mouse antibodies (7.5 μg/mL anti-OVA and 5 μg/mL anti-RBD) were captured on pre-conditioned anti-mouse IgG Fc-capture biosensors (AMC, Sartorius) for 600 s ...
-
bioRxiv - Immunology 2023Quote: All BLI for data shown in Figure 1 and Figure S1 was carried out on an OctetRED384 (ForteBio) using anti-human Fc-capture sensors (Sartorius, 18-5060) to immobilize anti-PfRH5 mAbs ...
-
bioRxiv - Microbiology 2022Quote: ... and BA.2+R493Q RBDs were diluted to 5 μg/mL and immobilized onto pre-hydrated streptavidin biosensors (Sartorius) to a level of 1 nm total shift ...
-
bioRxiv - Immunology 2022Quote: ... Anti-hIgG Fc capture (AHC) or anti-mIgG Fc capture sensors (Sartorius) were functionalized with monoclonal antibodies ...
-
bioRxiv - Biochemistry 2021Quote: ... RBD variants were biotinylated and immobilized on streptavidin biosensors (SA, Sartorius). Dilution series ranging from 5-0.625 nM of bipNbs were applied and one reference was included per run ...
-
bioRxiv - Immunology 2023Quote: ... biotinylated RBD (10 μg/mL) was immobilised on streptavidin biosensors (Sartorius) for 300 s ...
-
bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
-
bioRxiv - Microbiology 2020Quote: ... and D614G-S1 were measured using Forte Bio’s “Octet Red 96” BLI (Sartorius BioAnalytical Instruments ...
-
bioRxiv - Immunology 2022Quote: KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
-
bioRxiv - Biochemistry 2021Quote: ... Purified Spike was concentrated using a 100 kDa cut-off concentrator (Sartorius, GE) and loaded onto a HiLoad 16/60 superdex 200 pre-equilibrated with 20 mM Hepes ...
-
bioRxiv - Immunology 2022Quote: The binding affinity of obtained antibodies to RBD was examined by the Blitz system (Sartorius Japan) using protein A-coated biosensors ...
-
bioRxiv - Synthetic Biology 2022Quote: The binding kinetics of antibodies to RBD was measured by BLI on an Octet-R2 (Sartorius). The his-tagged RBD variants were loaded onto Ni-NTA biosensors from 10 ug\ml solution in kinetic buffer (PBS ...
-
bioRxiv - Immunology 2022Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb at a threshold of 0.8nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
-
bioRxiv - Biochemistry 2023Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb or FcACE2 to a threshold of 0.8 nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
-
bioRxiv - Immunology 2020Quote: ... SARS-2pp were concentrated 10-fold using 100,000 MWCO Vivaspin centrifugal concentrators (Sartorius) by centrifugation at 2000xg and stored at –80°C ...
-
bioRxiv - Bioengineering 2023Quote: ... Mouse Fc-capture biosensor tips were purchased from Sartorius. Chicken plasma (cat # ...
-
bioRxiv - Molecular Biology 2024Quote: ... Anti-human IgG Fc capture (AHC) biosensor tips (Sartorius) were equilibrated for ten minutes in a 1:4 mixture of conditioned Freestyle 293 medium and 1X HBS EP+ to match the diluted media ...
-
bioRxiv - Immunology 2023Quote: ... Antibodies were captured on anti-human Fc AHC2 tips (Sartorius). Data capture was performed using Octet Data Acquisition software version 9.0 (ForteBio) ...
-
bioRxiv - Immunology 2024Quote: ... 3C protease was removed using Ni-NTA resin and RBD-SD1 was buffer-exchanged into PBS and concentrated using 10,000 MWCO Vivaspin centrifugal spin columns (Sartorius).
-
bioRxiv - Biochemistry 2024Quote: Affinity of recombinant RBDs towards the purified ectodomain of TMPRSS2S441Awas assessed in real-time using a bio-layer interferometry Octet-R8 device (Sartorius). Nickel-NTA capture sensors (Sartorius ...
-
bioRxiv - Immunology 2021Quote: ... biotinylated S1 binding mAbs at 25 µg/mL were first loaded on High Precision Streptavidin SAX biosensors (Sartorius Corporation) for 10 sec ...
-
bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
-
bioRxiv - Immunology 2021Quote: ... IgGs were immobilized on Anti-hIgG Fc Capture (AHC) biosensors (Sartorius Corporation) and a concentration series of 200 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and captured on Anti-hIgG Fc Capture (AHC) Biosensors (Sartorius 18-5060). Capture was recorded for 40 seconds ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... IgG was loaded onto Anti-Human Fc Capture (AHC) biosensors (Sartorius, #18-5060). To reduce the avidity effect ...
-
bioRxiv - Molecular Biology 2022Quote: ... and fractions containing complete nanodiscs (Fig. S1) pooled and concentrated using a Vivaspin 500 PES 50 kDa cut off spin filter (Sartorius, Cat#VS0131).
-
bioRxiv - Biochemistry 2024Quote: ... seedlings were dry blotted on a paper towel to remove excess liquid media before measuring their fresh weight using a fine balance (Sartorius X64-S1). The relative seedling weight was calculated by dividing the seedling weight of each PAMP treated seedling by the average seedling weight of all mock-treated seedlings of the respective genotype.
-
bioRxiv - Microbiology 2023Quote: ... The tag-free RnpM protein was collected and concentrated using a Vivaspin turbo 15 (Sartorius, Germany).
-
bioRxiv - Immunology 2023Quote: ... BLI binding experiments were performed with Anti Human IgG Fc (AHC) biosensors (Sartorius 18-5060). Using Octet buffer (PBS with 0.1% Tween20) ...
-
bioRxiv - Microbiology 2022Quote: The affinity between each antibody and spike protein was measured by bio-layer interferometry (BLI) with a BLItz Biolayer Interferometer System (Sartorius, Goettingen, Germany).
-
bioRxiv - Immunology 2024Quote: ... Monoclonal Moo1 and Moo2 antibodies were immobilized on anti-human IgG Fc Capture (AHC) biosensors (Sartorius) to capture monoclonal antibodies at a concentration of 50 µg/mL for 5 min and washed with octet buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... The Hs53BP1 fragment without its GST tag was recovered in the flow through and concentrated using Vivaspin concentrator (Sartorius).
-
bioRxiv - Bioengineering 2023Quote: ... Antibody CSL362 hIgG1 (captured ligand) was captured by Anti-Human Fc capture biosensor (AHC) (Sartorius, PN: 18-5060) for 300s at 0.5µg/mL ...
-
bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
-
bioRxiv - Immunology 2023Quote: ... Human antibodies (5 μg/mL or 7.5 µg/mL) were captured on pre-conditioned anti-human IgG Fc-capture biosensors (AHC, Sartorius) for 600 s ...