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Citations for Sartorius :
1 - 50 of 254 citations for Recombinant Red Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Glioma cells were stably transduced with a lentiviral nuclear red fluorescent protein under the EF1a promotor (Sartorius, cat. 4476, Göttingen, Germany) for visualization in live imaging assays ...
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bioRxiv - Immunology 2021Quote: His-tagged FcαRI (Sinobiological, Cat: 10414-H08H) were immobilized via Ni-NTA biosensor (Sartorius, Cat: 18-5101) and subject to free floating IgA variants to obtained ka ...
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bioRxiv - Bioengineering 2022Quote: ... and HT29 cells were transfected to express the nuclear-restricted mKate2 red fluorescent protein using the IncuCyte® NucLight Lentivirus Reagents (Sartorius, Cat: 4476) and following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Purified His6-MBP-tagged proteins were then concentrated with a 30,000 MWCO centrifugal concentrator (Sartorius) and buffer exchanged into 20 mM HEPES pH 7.5 and 50 mM NaCl for crystallization ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Cancer Biology 2023Quote: ... Its binding to LIF was confirmed using recombinant LIF (ACRO Biosystems, LIF-H52H3) on an Octet RED 96e biolayer interferometry instrument (Sartorius).
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bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
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bioRxiv - Immunology 2021Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Systems Biology 2022Quote: ... live-cell proliferation and expression of YFP-tagged proteins were tracked for 48 h using an Incucyte S3 device (Sartorius).
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Immunology 2022Quote: The Octet quantification assay for protein expression level was performed on an Octet 96 Red system (Sartorius). Harvested cell media were centrifuged and cell supernatants were prepared in a 96-well plate with S-2P or S-6P standards diluted in media from 20 μg/mL to 0.3125 μg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... or HIS 1K biosensors (Sartorius/ForteBio) (CrossMAb ...
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bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
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bioRxiv - Immunology 2023Quote: ... Nuclight Red (Sartorius)-labeled LNCaP or DU145 were seeded at 12,000 cells/well in 100 µl phenol red-free RPMI in a 96-well plate and incubated for 18 hours ...
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bioRxiv - Molecular Biology 2023Quote: Antibody binding to Henipavirus F proteins was assessed using BLI on an Octet RED 384 (Sartorius, formerly ForteBio) with Forte Bio Kinetics Buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... and labeled using pHrodo red (Sartorius) at a final concentration of 31.3 ng/mL for 45 minutes at 37°C in pHrodo Labeling Buffer (Sartorius) ...
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bioRxiv - Bioengineering 2024Quote: ... The Cytotox red dye (Sartorius # 4632) for counting dead cells was added to the media at a final concentration of 100 nM ...
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bioRxiv - Bioengineering 2022Quote: ... or 1:1000 NucLight Rapid Red (Sartorius), in AIM V medium ...
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bioRxiv - Cancer Biology 2023Quote: ... and IncucyteⓇ Annexin V Red Dye (Sartorius, Germany) as markers of cell death and apoptosis ...
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bioRxiv - Cell Biology 2024Quote: ... Annexin V-red and Cytox-green reagents (Sartorius) were added according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2022Quote: ... and was captured by anti-Penta-His (HIS1K) sensors (Sartorius, 18-5120). The primary antibody was diluted to 150nM with a running buffer in wells ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were incubated for 3 hours with a red viability probe (1/3000, Incucyte® Nuclight Rapid Red Dye - Sartorius, Germany) incorporated by the nuclei of living cells ...
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bioRxiv - Cancer Biology 2022Quote: ... IncuCyte caspase-3/7 Red (cat#4704 from Sartorius) was added in the media ...
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bioRxiv - Cell Biology 2023Quote: ... IncuCyte Cytotox Red Dye (cat. #4632, Sartorius, Göttingen, Germany); Caspase 3 antibody (cat ...
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bioRxiv - Biochemistry 2022Quote: ... on an Octet Red 96 biolayer interferometer (Sartorius Bio). All experiments were performed in assay buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... or mCherry-tagged histone 2B (mCherry-H2B) were performed using an Incucyte SX5 (Sartorius Imaging). Images were taken once every 60 minutes to acquire videos of cells monitored over a 72-hour period.
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bioRxiv - Biochemistry 2021Quote: ... 12xHis-tagged EPCRWT or EPCRY154A was immobilized on the surface of NiNTA pre-coated biosensors (Sartorius), at a concentration of 100 µg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... including Incucyte® Nuclight Rapid Red Dye (Sartorius, cat. #: 4717) diluted 1:2000 ...
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bioRxiv - Biochemistry 2023Quote: BLI binding assays were performed on an Octet Red (Sartorius) instrument operated at 30℃ with shaking (1000 rpm) ...
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bioRxiv - Immunology 2024Quote: ... containing 0.1% BSA using Octet Red 96 (Sartorius, Goettingen, Germany). Biotinylated mAbs were immobilized on streptavidin-coated biosensors (Sartorius ...
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bioRxiv - Biochemistry 2024Quote: BLI binding assays were performed on an Octet Red (Sartorius) instrument operated at 30°C with shaking (1000 rpm) ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: MDA-MB-231-NR (NucLight red) cells (provided by Sartorius; RRID:CVCL_DF48) were transfected with FuGene HD reagent (Promega ...
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bioRxiv - Genetics 2023Quote: ... We also treated with Incucyte® Cytotox Red Dye (Sartorius, 4632) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Time-point fluorescent readings were then taken with an IncuCyte S3 (Sartorius-Germany) plate reader at 565-605 excitation and 624-705 emission for 60 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... green fluorescent signal was measured and quantified by IncuCyte S3 (Sartorius, Goettingen, Germany). The Image analysis was performed according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... green fluorescent signal was measured and quantified by IncuCyte S3 (Sartorius, Goettingen, Germany). To measure PD-1 protein on the cells ...
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bioRxiv - Immunology 2024Quote: ... 222 nM of 6xHis-tagged BG505 SOSIP was immobilized with nickel-charges tris-NTA (Ni-NTA) biosensors (Sartorius) for 10 min and transferred to PBS for 1 min to wash off unbound SOSIP ...
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bioRxiv - Immunology 2022Quote: Biolayer interferometry (BLI) was performed on an Octet Red 96e system (Sartorius). Streptavidin-coated biosensors were pre-hydrated with PBS-T (phosphate buffered saline pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... A 1:2,000 dilution of Incucyte® NucLight Red dye (Sartorius 4717) was added to stain nuclei and images were obtained every 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... the IncuCyte Caspase-3/7 Red apoptosis reagent (1.5 µM; #4704, Sartorius) was supplemented to the cultures ...
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bioRxiv - Immunology 2023Quote: ... and PASC-F with IncuCyte® NucLight Rapid Red Dye (4717, Sartorius). LTI-2355 ...
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bioRxiv - Immunology 2024Quote: ... Cells were transduced with the Incucyte Nuclight Red (mKate2) Lentivirus reagent (Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
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bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...