Labshake search
Citations for Sartorius :
1 - 50 of 278 citations for Recombinant Human CD22 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
-
bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
-
bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
-
bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
-
bioRxiv - Immunology 2021Quote: His-tagged FcαRI (Sinobiological, Cat: 10414-H08H) were immobilized via Ni-NTA biosensor (Sartorius, Cat: 18-5101) and subject to free floating IgA variants to obtained ka ...
-
bioRxiv - Immunology 2022Quote: KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
-
bioRxiv - Biochemistry 2022Quote: ... Purified His6-MBP-tagged proteins were then concentrated with a 30,000 MWCO centrifugal concentrator (Sartorius) and buffer exchanged into 20 mM HEPES pH 7.5 and 50 mM NaCl for crystallization ...
-
bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
-
bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
-
bioRxiv - Immunology 2022Quote: ... Protein-containing fractions were pooled and rebuffered to PBS using VIVAspin columns (5,000 MWCO PES; Sartorius). Protein yield was determined with a BCA protein assay (Pierce™ ...
-
bioRxiv - Microbiology 2023Quote: ... MTSL-labelled protein was concentrated (Vivaspin® 500 centrifugal filter, 10 kDa MWCO PES membrane, Sartorius). Labelling efficiencies for each Csx23 mutant were assessed by continuous wave (CW ...
-
bioRxiv - Immunology 2021Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
-
bioRxiv - Immunology 2022Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
-
bioRxiv - Systems Biology 2022Quote: ... live-cell proliferation and expression of YFP-tagged proteins were tracked for 48 h using an Incucyte S3 device (Sartorius).
-
bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
-
bioRxiv - Biochemistry 2022Quote: ... or HIS 1K biosensors (Sartorius/ForteBio) (CrossMAb ...
-
bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
-
bioRxiv - Microbiology 2020Quote: ... 10.000 MWCO PES (Sartorius). Finally ...
-
bioRxiv - Neuroscience 2022Quote: ... PES spin filters (Sartorius), then transferred to autosampler vials with 175μL inserts for analysis.
-
bioRxiv - Microbiology 2022Quote: ... The peak fractions corresponding to the protein complex were concentrated to about 16-20 mg/ml using a centrifugal filter with a PES membrane (Sartorius) and used for preparation of cryo-EM sample grids immediately.
-
bioRxiv - Molecular Biology 2023Quote: ... 10,000 MWCO PES columns (Sartorius). In brief ...
-
bioRxiv - Neuroscience 2020Quote: ... filtered using 0.4μm PES filters (Sartorius), concentrated 50-100 times and dialyzed into sterile Dulbecco’s phosphate buffer saline (DPBS ...
-
bioRxiv - Biochemistry 2020Quote: ... 10 kDa MWCO PES membranes (Sartorius); supplemented by NaCl ...
-
bioRxiv - Cancer Biology 2023Quote: ... and labeled using pHrodo red (Sartorius) at a final concentration of 31.3 ng/mL for 45 minutes at 37°C in pHrodo Labeling Buffer (Sartorius) ...
-
bioRxiv - Microbiology 2022Quote: ... 10,000 MWCO PES concentrator (Sartorius, catalog # VS0201) and Nanosep centrifugal devices with Omega membrane at MWCO of 30K (Pall ...
-
bioRxiv - Microbiology 2022Quote: ... 5000 MWCO PES (Sartorius AG, Göttigen, Germany) to 2 ml and separated using a HiLoad 16/60 Superdex 75 prep grade (Cytiva Europe GmbH ...
-
bioRxiv - Bioengineering 2022Quote: ... a 100 kDa MWCO Vivaspin PES filter (Sartorius) was pre-rinsed with deionized water to ensure full saturation of the filter membrane ...
-
bioRxiv - Biochemistry 2022Quote: ... The clear supernatant was microfiltered (0.22 µm, PES) and concentrated to 50 mL using a Sartorius Vivacell 250 PES Centrifugal Concentrator (Sartorius, Göttingen, Germany) with a MWCO of 100 kDa and a pressure of 4 bar ...
-
bioRxiv - Bioengineering 2022Quote: ... and was captured by anti-Penta-His (HIS1K) sensors (Sartorius, 18-5120). The primary antibody was diluted to 150nM with a running buffer in wells ...
-
bioRxiv - Biochemistry 2022Quote: ... using a 100-kDa MWCO Vivaspin Turbo PES (Sartorius) concentrator.
-
bioRxiv - Cell Biology 2022Quote: ... or mCherry-tagged histone 2B (mCherry-H2B) were performed using an Incucyte SX5 (Sartorius Imaging). Images were taken once every 60 minutes to acquire videos of cells monitored over a 72-hour period.
-
Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... and then filtered using a 300K Vivaspin 20 (PES, Sartorius) device at 2,000 g at 10 °C for 20–30 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... with Vivaflow 200 10,000 MWCO PES filters (Sartorius, NY, USA). Dm III-2 and Dm III-2 + cys were purified using Ni-NTA resin as described for Dm IV recombinant proteins (Suppl ...
-
bioRxiv - Biochemistry 2021Quote: ... 12xHis-tagged EPCRWT or EPCRY154A was immobilized on the surface of NiNTA pre-coated biosensors (Sartorius), at a concentration of 100 µg/mL ...
-
bioRxiv - Cancer Biology 2020Quote: ... Collected media was filtered using 0.45μm PES membrane (Minisart, Sartorius, Germany) to remove extracellular debris ...
-
bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
-
bioRxiv - Molecular Biology 2023Quote: ... The labeled tip was then dipped in 1x binding buffer (Sartorius) for 60 seconds to remove excess ligand and achieve baseline ...
-
bioRxiv - Plant Biology 2020Quote: ... and passed through 0.8 μM PES spin filters (Vivaclear, Sartorius Stedim Biotech) before being run on the LC-MS/MS ...
-
bioRxiv - Pathology 2023Quote: ... Supernatants were collected and passed through an 0.8 μm PES filter (Sartorius) for the removal of smaller debris and bacteria ...
-
bioRxiv - Immunology 2024Quote: ... 222 nM of 6xHis-tagged BG505 SOSIP was immobilized with nickel-charges tris-NTA (Ni-NTA) biosensors (Sartorius) for 10 min and transferred to PBS for 1 min to wash off unbound SOSIP ...
-
bioRxiv - Biophysics 2020Quote: ... 0.32μM DNA) and loaded on centrifugal concentrators (Vivaspin 500; 10,000 MWCO PES, Sartorius). The quenched samples were concentrated to 60 μl by centrifugation at 20,000xg for 2 min (Sigma 1-16K) ...
-
bioRxiv - Microbiology 2019Quote: Soil pH was determined with a compound electrode (PE 10, Sartorius, Goettingen, Germany) using a soil/water ratio of 1:2.5(w/v ...
-
bioRxiv - Microbiology 2023Quote: ... and the resulting supernatant was passed through a 0.8 µm PES filter (Sartorius). A nuclease treatment was then performed using micrococcal nuclease (New England Biolabs ...
-
bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...
-
bioRxiv - Biochemistry 2021Quote: ... The buffer was filtered with a 0.22 μm Minisart® PES syringe filter (Sartorius).
-
bioRxiv - Microbiology 2022Quote: ... P116 was concentrated with Vivaspin 500 centrifugal concentrators (10,000 MWCO PES, Sartorius, Göttingen, Germany) to a final concentration of >0.5 mg/mL.
-
bioRxiv - Microbiology 2023Quote: ... The 0.45 µm filtrate was concentrated using tangential filtration (100’000 MWCO PES Sartorius ®) and analysed via flow cytometry to detect viral populations in the sample (see SI for flow cytometry description).
-
bioRxiv - Bioengineering 2023Quote: ... After sterile filtration through 0.45|0.2-micron PES Sartopore 2 filter (Sartorius Stedim, Germany) the formulated bulk sample was collected in 3 L Flexboy 2D bag (Sartorius Stedim ...
-
bioRxiv - Microbiology 2023Quote: ... the samples were concentrated using filter columns (Vivaspin 6, 100,000 MWCO PES, Sartorius, Germany) at 4 °C with a maximal speed of 4,000 x g and washed twice with DBCM2 salt solution ...
-
bioRxiv - Immunology 2022Quote: ... It was then diluted through a 100 k MWCO PES membrane (Sartorius Stedim Biotech) against a 10-fold volume of DPBS (pH 7.4 ...