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Citations for Sartorius :
1 - 50 of 171 citations for RapidClean Protein Removal kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... After removal of the debris using a Vivaclear Mini column (Sartorius), the cell extract ...
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bioRxiv - Molecular Biology 2021Quote: ... particulate contaminant removal and further sterile filtered using 0.2 µm Sartopore 2 filters (Sartorius), then divided into four parts for further processing ...
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bioRxiv - Biochemistry 2022Quote: ... followed by the removal of free dye through 10 kDa Vivaspin® 500 centrifugal concentrators (Sartorius Stedim Biotech ...
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bioRxiv - Molecular Biology 2023Quote: The MultiCyt® QBeads® PlexScreen Secreted Protein Assay Kit (Sartorius) was used to quantify the level of tumor necrosis factor alpha (TNF-a ...
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bioRxiv - Immunology 2022Quote: ... followed by the removal of 2-MEA by buffer-exchanging to PBS using 100kDa Vivaspin6 columns (Sartorius) and storing overnight at 4 °C to allow for reoxidation of the disulfide bonds.
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated for 16 h at 37 °C following removal of RiGH26 using a Vivaspin 20 filtration unit (10.000 MWCO PES, Sartorius) and carbohydrate lyophilization on an ALPHA 2-4 LD Plus freeze dryer (Christ ...
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bioRxiv - Immunology 2020Quote: ... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
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bioRxiv - Biochemistry 2023Quote: ... each Protein A biosensors (Sartorius) was pre-equilibrated in PBST buffer (PBS ...
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bioRxiv - Immunology 2022Quote: ... Protein A biosensors (Sartorius, #18-5010) were hydrated in BLI assay buffer for 10-20 minutes and loaded onto an Octet Red96 BLI instrument (Pall ...
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bioRxiv - Microbiology 2019Quote: ... Residual proteins were then eliminated by filtration on 0.22-µm high-protein-retention Ministart syringe filters (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Neuroscience 2020Quote: ... using protein concentrator tubes (VIVASPIN 20, Sartorius). Both purified IgG fractions from patient and controls were re-tested on CBAs and the anti-IgLON5 IgG concentration was determined (titer 1:100) ...
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bioRxiv - Cancer Biology 2020Quote: Proteins were transferred to nitrocellulose membrane (Sartorius). The membranes were blocked with 10% BSA (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were concentrated using centrifugal concentrator (Sartorius) and further purified with size exclusion chromatography using a Superdex 200 column (GE Life Sciences) ...
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bioRxiv - Microbiology 2023Quote: ... The eluted proteins were concentrated with a centrifugal device (MWCO 30 kDa for soluble EFNB2 proteins and 50 kDa for soluble Eph receptor proteins; Sartorius) before being separated on a Superdex 200 Increase 10/300 GL (Cytiva Life Sciences ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated using a Vivaspin concentrator (Sartorius) with a half lower MWCO than the size of the protein being purified ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using Vivaspin centrifugal filters (Sartorius) of appropriate cut-offs ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated with VIVASPIN concentrators (Sartorius) and dialyzed against E-Buffer with 1 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... using a 100kDa MWCO Vivaspin protein concentrator (Sartorius), aliquoted ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated using Vivaspin concentrators (Sartorius Stedin) and the sample purity and homogeneity was assessed by SDS-PAGE.
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bioRxiv - Biophysics 2021Quote: ... Eluted protein was concentrated using centrifugal concentrators (VivaSpin, Sartorius) and then assessed for successful labeling by measuring fluorescence using the following settings and the monochromator in the BioTek Synergy Neo2 plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... 2mM DTT and proteins concentrated via centrifugation (Vivaspin, Sartorius). Recombinant kinase was added to 10µl of 2X kinase buffer [40mM Hepes (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... All protein samples were concentrated using Vivaspin centrifugal concentrators (Sartorius). Denaturing gel electrophoresis was performed using NuPAGE 4-12 % Bis-Tris gels (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Then proteins were transferred into ultrafiltration tubes (10kDa, Sartorius AG) and washed three times with 50 mM NH4HCO3 at 14000g for 15 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using centrifugal filter devices (Vivaspin, Sartorius). Protein concentration was estimated from its absorbance at 280 nm ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was then concentrated (Vivaspin 30,000 MWCO, Sartorius) and ultracentrifuged in a TLA-120.2 rotor (Beckman Coulter ...
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bioRxiv - Bioengineering 2024Quote: ... the refolded protein was concentrated using a VIVAFLOW50 system (Sartorius). The concentrated sample was filtrated with 0.22 µm membrane filter ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were concentrated using 30 kDa cut-off Vivaspin columns (Sartorius) and their concentration was determined by their absorbance at 280 nm.
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bioRxiv - Microbiology 2019Quote: ... Protein was concentrated to ~ 5ml using Vivaspin concentrator spin columns (Sartorius) at 4500 × ɡ ...
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bioRxiv - Cell Biology 2019Quote: ... the protein was concentrated after labelling using a Vivaspin concentrator (Sartorius), ultra-centrifuged (280.000 x g ...
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bioRxiv - Microbiology 2019Quote: ... Protein was concentrated to ~ 5ml using Vivaspin concentrator spin columns (Sartorius) at 4500 × ɡ ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was concentrated to 32 mg/ml using centrifugal concentrators (Sartorius) with a relative molecular mass cut-off of 100K.
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bioRxiv - Synthetic Biology 2021Quote: ... Protein was concentrated using a Vivaspin 500 column (Sartorius, Goettingen, Germany) column following the manufacturer’s instructions.
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Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery systembioRxiv - Genetics 2019Quote: ... filtered using a 0.45 µm low protein retention syringe filter (Sartorius) and 0.5 mL aliquots were stored at −80 °C.
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by ultrafiltration (3 kDa Vivaspin; Sartorius, Göttingen, Germany). ACE and ANSA hydrolysis activities were quantified in discontinuous HPLC-based assays at 30°C in lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... All proteins were diluted in Octet kinetics buffer (Sartorius, Göttingen, Germany). Antibodies were captured on anti-human Fc AHC2 tips (Sartorius) ...
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bioRxiv - Microbiology 2023Quote: ... The purified proteins were concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 5 or 50 kDa) ...
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bioRxiv - Cancer Biology 2024Quote: ... concentrated 10x using Vivaspin™ protein concentrator spin columns (Sartorius, VS15T02) and stored at 4°C.
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bioRxiv - Biochemistry 2020Quote: ... Protein was concentrated using a Vivaspin centrifugal concentrator (MWCO 10 kDa, Sartorius) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The protein was concentrated with Vivaspin 20 concentrators (30 kDa MWCO, Sartorius). Concentrated protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (Cytiva) ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were centrifuged on a 30 kDa cutoff filter device (Sartorius) and washed each thrice with UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialysed protein was spin-concentrated in a Vivaspin 6 MWCO 50,000 (Sartorius) to the desired concentration ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were concentrated using a Vivaspin6 10 kDa filter unit (Sartorius). The protein concentration was measured via absorption at 280 nm using a Nanodrop spectrophotometer (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted protein was adjusted to desired concentration via Vivaspin (Sartorius, Göttingen, Germany), analyzed by SDS-PAGE and snap frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... followed by centrifugation of denatured proteins on a filter device (Sartorius, Vivacon 500). After several washing steps with 8 M urea and ammonium bicarbonate ...
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bioRxiv - Biochemistry 2021Quote: ... The immobilization of ligand proteins on amine reactive second-generation (AR2G) biosensors (Sartorius) was done following manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted to 100 nM in Kinetics buffer (Pall FortéBio/Sartorius). Buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated using ultrafiltration through a 30 kDa cutoff filter (Sartorius), snap-frozen and stored at −80 °C until further used.