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Citations for Sartorius :
1 - 50 of 199 citations for Mayaro Virus E2 Envelope Protein Mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Mouse Fc-capture biosensor tips were purchased from Sartorius. Chicken plasma (cat # ...
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bioRxiv - Microbiology 2023Quote: ... The tag-free RnpM protein was collected and concentrated using a Vivaspin turbo 15 (Sartorius, Germany).
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bioRxiv - Immunology 2022Quote: ... Anti-hIgG Fc capture (AHC) or anti-mIgG Fc capture sensors (Sartorius) were functionalized with monoclonal antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
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bioRxiv - Immunology 2023Quote: ... Mouse antibodies (7.5 μg/mL anti-OVA and 5 μg/mL anti-RBD) were captured on pre-conditioned anti-mouse IgG Fc-capture biosensors (AMC, Sartorius) for 600 s ...
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bioRxiv - Molecular Biology 2020Quote: ... The collected virus was filtered (0.45 μm filter, Sartorius) and concentrated using PEG-it overnight at 4°C (SBI Integrated Sciences ...
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bioRxiv - Microbiology 2022Quote: ... Infectious virus titer was determined using an Incucyte system (Sartorius) to quantify the red fluorescence from the mCherry reporter signal as detailed above ...
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bioRxiv - Immunology 2022Quote: KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
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bioRxiv - Immunology 2022Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb at a threshold of 0.8nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb or FcACE2 to a threshold of 0.8 nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Microbiology 2021Quote: ... and virus particles were enriched in Vivaspin 20 columns (Sartorius Stedim, Biotech) via centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... the virus supernatant was concentrated using Vivaspin 20 concentrators (Sartorius Stedim Biotech) by spinning at 6000 g for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... virus was concentrated using the 100000 MWCO Vivaspin® Concentrator (Sartorius Stedim) and titrated on mammary epithelial cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Anti-human IgG Fc capture (AHC) biosensor tips (Sartorius) were equilibrated for ten minutes in a 1:4 mixture of conditioned Freestyle 293 medium and 1X HBS EP+ to match the diluted media ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated by centrifugation in a 100,000 MWCO ultrafiltration unit (Vivaspin, Sartorius). Titers of virus stocks were determined by plaque assay on BV2 cells(57).
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bioRxiv - Immunology 2023Quote: ... Antibodies were captured on anti-human Fc AHC2 tips (Sartorius). Data capture was performed using Octet Data Acquisition software version 9.0 (ForteBio) ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was amplified and purified using AdenoPACK 20 Adenovirus (Ad5) purification & concentration kit (Sartorius).
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bioRxiv - Developmental Biology 2020Quote: ... 10 mL of virus supernatant was filtered through a 0.45 um filter (Sartorius, 16533) and supplemented with 8µg/ml polybrene (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... the virus-containing medium was filtered through a 0.45 µm membrane filter (Sartorius®) after 2.5 days ...
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bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
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bioRxiv - Immunology 2021Quote: ... IgGs were immobilized on Anti-hIgG Fc Capture (AHC) biosensors (Sartorius Corporation) and a concentration series of 200 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and captured on Anti-hIgG Fc Capture (AHC) Biosensors (Sartorius 18-5060). Capture was recorded for 40 seconds ...
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bioRxiv - Molecular Biology 2019Quote: Live cells monitoring of virus infection was performed using the IncuCyte Live-Cell Analysis system (Sartorius). Cells were seeded in a 24-well plated at 80% confluency ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested and virus particles were purified from cytokines and concentrated using Vivaspin 20 (Sartorius, filtration units with a size exclusion of 100 kDa ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 mL of virus supernatant were harvested by filtering through a 0.45 um filter (Sartorius, 16533) and supplemented with 8μg/ml polybrene (Millpore ...
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bioRxiv - Evolutionary Biology 2022Quote: ... IgG was loaded onto Anti-Human Fc Capture (AHC) biosensors (Sartorius, #18-5060). To reduce the avidity effect ...
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bioRxiv - Systems Biology 2020Quote: ... virus was grown on Vero E6 cells and concentrated using Vivaspin® 20 concentrators (Sartorius Stedim Biotech). Virus stocks were stored at −80°C ...
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bioRxiv - Molecular Biology 2022Quote: ... the virus was purified using an Adenovirus (Ad5) Purification and Concentration Kit (AdenoPACK 20; Sartorius, Goettingen, Germany) according to the manufacturer’s instructions and stored at −80 °C until use ...
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bioRxiv - Immunology 2020Quote: ... the supernatant containing virus particles was harvested and filtered with a 0.45 μm syringe filter (Sartorius Stedim Biotech). Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then passed through 0.22 μm filter followed by 20 nm ViroSart virus filter (Sartorius Stedim, Germany). The clarified samples were then reconstituted in formulation buffer ...
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bioRxiv - Immunology 2023Quote: ... BLI binding experiments were performed with Anti Human IgG Fc (AHC) biosensors (Sartorius 18-5060). Using Octet buffer (PBS with 0.1% Tween20) ...
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bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Immunology 2024Quote: ... Monoclonal Moo1 and Moo2 antibodies were immobilized on anti-human IgG Fc Capture (AHC) biosensors (Sartorius) to capture monoclonal antibodies at a concentration of 50 µg/mL for 5 min and washed with octet buffer ...
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bioRxiv - Microbiology 2022Quote: The filtration efficiency of AF-Fe against virus-loaded aerosols was assessed using a compact experimental setup composed mainly of a polycarbonate filtration holder (Sartorius, Germany) connected to three 1.5 l compressed air tanks (PanGas ...
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bioRxiv - Molecular Biology 2023Quote: ... The Hs53BP1 fragment without its GST tag was recovered in the flow through and concentrated using Vivaspin concentrator (Sartorius).
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bioRxiv - Neuroscience 2023Quote: ... The recovered virus fraction was purified by first passing through a 100-kDA molecular weight cut-off (MWCO) centrifugal filter (Sartorius VIVASPIN VS2041) and then through an Amicon Ultra 2ml Centrifugal filter (Millipore UFC210024) ...
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bioRxiv - Neuroscience 2024Quote: ... The recovered virus fraction was purified by first passing through a 100 kDa molecular weight cutoff (MWCO) centrifugal filter (Sartorius, VIVASPIN VS2041) and then through an Amicon Ultra 2mL Centrifugal filter (Millipore #UFC210024) ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: ... The recovered virus fraction was purified by first passing through a 100 kDa molecular weight cutoff (MWCO) centrifugal filter (Sartorius, VIVASPIN VS2041) and then through an Amicon Ultra 2 ml Centrifugal filter (Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... Antibody CSL362 hIgG1 (captured ligand) was captured by Anti-Human Fc capture biosensor (AHC) (Sartorius, PN: 18-5060) for 300s at 0.5µg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... The recovered virus fraction was purified by first passing through a 100-kDa molecular weight cut off (MWCO) centrifugal filter (Sartorius Vivaspin, Cat# VS2041) and then through an Amicon Ultra 2ml Centrifugal filters (Merck Millipore ...
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bioRxiv - Immunology 2023Quote: ... Human antibodies (5 μg/mL or 7.5 µg/mL) were captured on pre-conditioned anti-human IgG Fc-capture biosensors (AHC, Sartorius) for 600 s ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% nonfat dry milk) to 100 nM and immobilized for 60 s to anti-human IgG Fc capture biosensors (Sartorius). Sensors were transferred to assay buffer for 30 s to set the baseline ...
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bioRxiv - Immunology 2020Quote: ... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...
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bioRxiv - Biochemistry 2023Quote: ... each Protein A biosensors (Sartorius) was pre-equilibrated in PBST buffer (PBS ...
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bioRxiv - Immunology 2022Quote: ... Protein A biosensors (Sartorius, #18-5010) were hydrated in BLI assay buffer for 10-20 minutes and loaded onto an Octet Red96 BLI instrument (Pall ...
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bioRxiv - Biochemistry 2022Quote: ... In our BLI experiments, ACE2 Fc-fusions are tethered to either Streptavidin biosensors (WT-ACE2, CVD293) (Sartorius®, Item no.: 18-5019) or ProtA biosensors (Sartorius® ...
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bioRxiv - Immunology 2023Quote: All BLI for data shown in Figure 1 and Figure S1 was carried out on an OctetRED384 (ForteBio) using anti-human Fc-capture sensors (Sartorius, 18-5060) to immobilize anti-PfRH5 mAbs ...