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Citations for Sartorius :
1 - 29 of 29 citations for Ion Exchange Chromatography since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Purified viral particles were obtained by Ion Exchange (VivaPure® AdenoPACKTM 20, Sartorius), and the DNA extracted and subjected to Next Generation Sequencing ...
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bioRxiv - Immunology 2021Quote: ... The TFF retentate was next enriched for AAV particles on a cation exchange chromatography column (BIA Separations, Sartorius). The eluate was concentrated ...
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bioRxiv - Bioengineering 2021Quote: ... Virus was partially purified using depth filtration and anion exchange chromatography with syringes and small-scale filters (C0SP 23 cm2 [Merck] and Sartobind Q Nano [Sartorius]). Buffer compositions and other parameters were as previously described but scaled proportionately to the product and filter sizes (5) ...
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bioRxiv - Synthetic Biology 2022Quote: ... pure Q-GRFT was prepared by flow-through membrane chromatography on a salt-tolerant anion exchange capsule (Sartobind STIC PA nano, Sartorius, Goettingen, Germany) according to manufacturer instructions.
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bioRxiv - Molecular Biology 2022Quote: ... Buffer exchange was accomplished using Vivaspin concentrators (Sartorius). Protein purity was assessed by SDS-PAGE followed by Coomassie blue staining ...
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bioRxiv - Microbiology 2023Quote: ... and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Microbiology 2024Quote: ... and buffer exchange to PBS using VivaSpin columns (Sartorius) was carried out ...
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bioRxiv - Biochemistry 2020Quote: ... Imidazole was removed by buffer exchange using a Vivaspin centrifugal filter (Sartorius) with a 10 kDa cut-off ...
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bioRxiv - Immunology 2022Quote: ... A CIMac pDNA 0.3 mL weak anion-exchange analytical column (Sartorius, Germany) was used with a Shimadzu
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bioRxiv - Microbiology 2023Quote: ... After two buffer exchanges with trypsin buffer on Vivaspin 15R (5 kDa) columns (Sartorius), the samples were digested by trypsin with 1/80 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... followed by concentrating and buffer exchange into IMAC binding buffer using Vivaflow 100 system (Sartorius). The resulting protein solution was purified by Histrap column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... Buffer exchange and protein sample concentration were performed using 10 kDa MWCO Vivaspin Turbo centricons (Sartorius, Germany). After IMAC ...
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bioRxiv - Microbiology 2020Quote: ... For all recombinant proteins,protein-containing fractions were pooled and buffer exchange to PBS via VivaSpin columns (Sartorius) was performed ...
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bioRxiv - Neuroscience 2022Quote: ... Buffer exchange and concentration was done with Vivaspin 20 ultrafiltration unit having a 1.000.000 molecular weight cut-off (Sartorius). Viral particles were washed and resuspended in PBS and titered using quantitative PCR with primers targeting WPRE element.
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated toxins were purified using buffer exchange columns (Vivacon 500, Sartorius, 3000 Da Molecular Weight Cut-Off) using the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... The capped mRNA underwent further purification through oligo-dT chromatography (Sartorius) before being dissolved in 10 mM Citrate buffer at pH 4.0 ...
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bioRxiv - Biochemistry 2020Quote: ... 5% ethylene glycol), followed by buffer exchange to (30 mM Tris/HCl, pH 8.0) with Vivaspin centrifugal concentrator (Sartorius, Germany). The final phosphoprotein concentrations were adjusted to 1.5 mg/ml (80 μM) ...
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bioRxiv - Biophysics 2020Quote: ... MgCl2 and EGTA were removed by 4 times buffer exchange using a Vivaspin 500.5 molecular weight cut-off (Sartorius, Göttingen). Samples were loaded onto a SDS-PAGE gel to confirm phosphorylation.
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bioRxiv - Cell Biology 2024Quote: ... Buffer exchange from iodixanol to Ringer’s lactate buffer was then carried out using Vivaspin 20 columns (Sartorius, VS2042, Göttingen, Germany). To obtain high virus titers ...
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bioRxiv - Cell Biology 2022Quote: ... GCP6-tagged purified γ-TuRC was then subjected to buffer exchange using Vivaspin 500 centrifugal concentrator (10 kDa MWCO, Sartorius VS0102) for a final NaCl concentration of 150 mM in eluate ...
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bioRxiv - Plant Biology 2023Quote: ... Finally, protein concentration and buffer exchange (20 mM Tris-HCl, pH 7.5) was performed using Vivaspin 30K columns (Sartorius, Germany, Göttingen, Germany).
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bioRxiv - Immunology 2022Quote: ... The affinity chromatography eluate was subjected to concentration and diafiltration using TFF (Sartorius Stedim Biotech GmbH). In order to produce the immunoaffinity resin for PF-D-Trimer purification ...
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bioRxiv - Microbiology 2024Quote: ... HPLC (High Performance Liquid Chromatography) amino acid analysis of the supernatants was carried out by Sartorius Xell GmbH (Göttingen ...
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bioRxiv - Microbiology 2024Quote: ... pooled enzyme samples after affinity chromatography were concentrated using centrifugal concentrators (10 kDa cutoff) (Sartorius, Germany) and applied to size exclusion chromatography (Superdex 200 HiLoad 26/60 prep grade column or Superose 6/100 column (Cytavia ...
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bioRxiv - Bioengineering 2020Quote: ... The AAV2/8 was purified by affinity chromatography and concentrated using ultrafiltration columns (Sartorius Stedim Biotech, Goettingen, Germany), washed in PBS and concentrated to a volume of 100–150 µl ...
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bioRxiv - Immunology 2022Quote: ... The immunoaffinity chromatography eluate was subjected to concentration and diafiltration using tangential flow filtration (TFF) (Sartorius Stedim Biotech GmbH) and final 20 nm nanofiltration (Pall Corporation ...
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bioRxiv - Biochemistry 2020Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and Ub/LC3B-PA was concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C.
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bioRxiv - Biochemistry 2023Quote: ... Unreacted propargylamine was removed by size exclusion chromatography and the probes were concentrated using VIVASPIN 20 Columns (3 kDa cutoff, Sartorius), flash frozen and stored at −80 °C ...
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bioRxiv - Microbiology 2024Quote: ... the fractions obtained by affinity chromatography were pooled and concentrated using conical membrane ultrafiltration tubes (Vivaspin 6 mL 10 kDa MWCO, Sartorius) to 7 mg/mL (2 mL) ...