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Citations for Sartorius :
1 - 50 of 158 citations for Inositol monophosphatase 1 IMPA1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2022Quote: ... or HIS 1K biosensors (Sartorius/ForteBio) (CrossMAb ...
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bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
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bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
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bioRxiv - Bioengineering 2022Quote: ... and was captured by anti-Penta-His (HIS1K) sensors (Sartorius, 18-5120). The primary antibody was diluted to 150nM with a running buffer in wells ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: His-tagged FcαRI (Sinobiological, Cat: 10414-H08H) were immobilized via Ni-NTA biosensor (Sartorius, Cat: 18-5101) and subject to free floating IgA variants to obtained ka ...
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bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... His-PKR was further concentrated to at least 10 mg/ml using Vivaspin centrifugal concentrator 30 K (Sartorius), snap-frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Immunology 2022Quote: KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg/ml in Kinetics buffer (PBS 0.01% BSA) and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biophysics 2024Quote: ... The 50 ml containing 6-His-AdhE was concentrated to a final volume of 10.5 ml using a Vivaspin 20 centrifugal concentrator (Sartorius) with a molecular weight cut-off of 30 kDa and then dialysed against buffer A ...
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bioRxiv - Immunology 2022Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb at a threshold of 0.8nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb or FcACE2 to a threshold of 0.8 nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Immunology 2023Quote: All BLI for data shown in Figure 1 and Figure S1 was carried out on an OctetRED384 (ForteBio) using anti-human Fc-capture sensors (Sartorius, 18-5060) to immobilize anti-PfRH5 mAbs ...
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bioRxiv - Molecular Biology 2024Quote: ... Anti-human IgG Fc capture (AHC) biosensor tips (Sartorius) were equilibrated for ten minutes in a 1:4 mixture of conditioned Freestyle 293 medium and 1X HBS EP+ to match the diluted media ...
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bioRxiv - Biochemistry 2022Quote: ... IgGs produced from the scFvs from the above sort were assessed for their competition of binding with one another using anti-Penta HIS (His1K) biosensors (Sartorius/ForteBio). His1K tips were pre-quenched with buffer containing 10nM biotin ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were captured on anti-human Fc AHC2 tips (Sartorius). Data capture was performed using Octet Data Acquisition software version 9.0 (ForteBio) ...
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bioRxiv - Molecular Biology 2023Quote: Human NIL iPSCs were cultured in Nutristem (Sartorius, #05-100-1A) supplemented with Pen/Strep 0.1% on geltrex-coated plates (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Beads detecting cytokines from human were analyzed on an IQue Screener Plus (Sartorius). All assays were analyzed using quintuplicate determinations.
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bioRxiv - Evolutionary Biology 2022Quote: ... IgG was loaded onto Anti-Human Fc Capture (AHC) biosensors (Sartorius, #18-5060). To reduce the avidity effect ...
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bioRxiv - Molecular Biology 2022Quote: Human primary keratinocytes were plated in Essen ImageLock 96-well plates (Sartorius, Göttingen, Germany) at 15,000 cells per well for migration assay and in 12-well plates at 20,000 cells per well for cell growth assay ...
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bioRxiv - Cancer Biology 2023Quote: The human plasma samples were filtered (Minisart NML syringe filters, pore size: 0.8 µm; Sartorius) and diluted with 1x Phosphate-Buffered Saline without calcium and magnesium buffer (Gibco ...
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bioRxiv - Immunology 2023Quote: ... BLI binding experiments were performed with Anti Human IgG Fc (AHC) biosensors (Sartorius 18-5060). Using Octet buffer (PBS with 0.1% Tween20) ...
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bioRxiv - Microbiology 2023Quote: We performed human plasma and avidity variant Abs’ binding using an Octet Red384 biolayer interferometer (Sartorius). We completed all the steps in 0.05% BSA and 0.002% Tween20 ...
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bioRxiv - Immunology 2024Quote: ... Monoclonal Moo1 and Moo2 antibodies were immobilized on anti-human IgG Fc Capture (AHC) biosensors (Sartorius) to capture monoclonal antibodies at a concentration of 50 µg/mL for 5 min and washed with octet buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Antibody CSL362 hIgG1 (captured ligand) was captured by Anti-Human Fc capture biosensor (AHC) (Sartorius, PN: 18-5060) for 300s at 0.5µg/mL ...
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bioRxiv - Immunology 2023Quote: ... Human antibodies (5 μg/mL or 7.5 µg/mL) were captured on pre-conditioned anti-human IgG Fc-capture biosensors (AHC, Sartorius) for 600 s ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% nonfat dry milk) to 100 nM and immobilized for 60 s to anti-human IgG Fc capture biosensors (Sartorius). Sensors were transferred to assay buffer for 30 s to set the baseline ...
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bioRxiv - Cell Biology 2023Quote: ... 1% penicillin/streptomycin (PEN-STREP) and 1% L-glutamine (Sartorius). Cells were incubated at 37C ...
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bioRxiv - Microbiology 2023Quote: ... cell-free media was prepared for the LC-MS run by dilution in 1:1 in acetonitrile at a final volume of 1 ml and passed through 0.2 μm filters (Sartorius). Analysis of TPA ...
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Enhancer heterogeneity of lung neuroendocrine tumors reveals sensitivity to FGF signaling inhibitionbioRxiv - Cancer Biology 2023Quote: ... 1% sodium pyruvate (Sartorius), and 1% non-essential amino acids (Diagnovum) ...
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bioRxiv - Molecular Biology 2023Quote: ... For the Incucyte assay, THP-1 or Jeko-1 cells were transduced with IncuCyte NucLight Red Lentivirus Reagent (EF-1 Alpha, Puro) (Sartorius, USA). All cell lines were repeatedly tested for the presence of mycoplasma contamination with a MycoReport Mycoplasma Detection Kit (Evrogen ...
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bioRxiv - Genetics 2023Quote: ... and 1% L-glutamine (Sartorius) and passaged every 2–4 days.
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Enhancer heterogeneity of lung neuroendocrine tumors reveals sensitivity to FGF signaling inhibitionbioRxiv - Cancer Biology 2023Quote: ... 1% L-glutamine (Sartorius, Göttingen, Germany), 1% sodium pyruvate (Sartorius) ...
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bioRxiv - Biophysics 2024Quote: ... The acquired raw data for the binding of anti-TcPOP mAbs with TcPOP were processed and globally fit to a 1:1 binding model with Octet Analysis Studio 12.2.2.26 Software (Sartorius). The binding kinetics measurements were carried out in three replicates ...
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bioRxiv - Genomics 2022Quote: ... diluted to OD600 = 0.5 and mixed in a 1:1 ratio and spotted onto 0.22 µm nitrocellulose membrane (Sartorius) placed on top of LBA (20 µL) ...
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bioRxiv - Biochemistry 2022Quote: ... Purified yCAF-1 complexes (Figure 1- figure supplement 1a) were concentrated with 100 000 MWCO centrifugal concentrators (Sartorius), aliquoted in small volumes ...
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bioRxiv - Plant Biology 2019Quote: ... BT125D analytical balance 1/100,000 from Sartorius Company ...
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bioRxiv - Bioengineering 2022Quote: ... or 1:1000 NucLight Rapid Red (Sartorius), in AIM V medium ...
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bioRxiv - Bioengineering 2023Quote: ... Analysis and kinetic curve fitting (assuming a 1:1 binding model) were conducted using Octet Data Analysis HT software version 7.1 (Sartorius). Normalized equilibrium binding curves were obtained by plotting the response value at the end of the association phase for each sample dilution and normalizing to the maximum value ...