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Citations for Sartorius :
1 - 50 of 336 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade B 89BZ_167 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The collected virus was filtered (0.45 μm filter, Sartorius) and concentrated using PEG-it overnight at 4°C (SBI Integrated Sciences ...
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bioRxiv - Microbiology 2022Quote: ... Infectious virus titer was determined using an Incucyte system (Sartorius) to quantify the red fluorescence from the mCherry reporter signal as detailed above ...
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bioRxiv - Microbiology 2023Quote: ... 2 L or 5 L scale (Biostat B-plus and B-DCU units, Sartorius Stedium Biotech) as previously described [PMID ...
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bioRxiv - Microbiology 2021Quote: ... and virus particles were enriched in Vivaspin 20 columns (Sartorius Stedim, Biotech) via centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... the virus supernatant was concentrated using Vivaspin 20 concentrators (Sartorius Stedim Biotech) by spinning at 6000 g for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... virus was concentrated using the 100000 MWCO Vivaspin® Concentrator (Sartorius Stedim) and titrated on mammary epithelial cells ...
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bioRxiv - Microbiology 2020Quote: A BIOSTAT® B bioreactor (Sartorius Stedim) with a 1.5-L capacity UniVessel® glass vessel was used for growth of 800-mL cultures at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated by centrifugation in a 100,000 MWCO ultrafiltration unit (Vivaspin, Sartorius). Titers of virus stocks were determined by plaque assay on BV2 cells(57).
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bioRxiv - Synthetic Biology 2022Quote: ... The seed culture from each flask was transferred to a 1-L bioreactor (Biostat B-DCU, Sartorius, Germany) with 0.7 L fermentation medium ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was amplified and purified using AdenoPACK 20 Adenovirus (Ad5) purification & concentration kit (Sartorius).
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bioRxiv - Developmental Biology 2020Quote: ... 10 mL of virus supernatant was filtered through a 0.45 um filter (Sartorius, 16533) and supplemented with 8µg/ml polybrene (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... the virus-containing medium was filtered through a 0.45 µm membrane filter (Sartorius®) after 2.5 days ...
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bioRxiv - Zoology 2022Quote: ... and 500 µL 10X Amphotericin B (AmB) Solution (Sartorius) for 3 times ...
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bioRxiv - Immunology 2021Quote: ... IncuCyte® Annexin V Green Reagent was added directly in the medium at the dilution 1:200 and B cells were imaged every hour for 3.5 days using IncuCyte® Live-Cell Analysis System (Sartorius).
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bioRxiv - Molecular Biology 2019Quote: Live cells monitoring of virus infection was performed using the IncuCyte Live-Cell Analysis system (Sartorius). Cells were seeded in a 24-well plated at 80% confluency ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested and virus particles were purified from cytokines and concentrated using Vivaspin 20 (Sartorius, filtration units with a size exclusion of 100 kDa ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 mL of virus supernatant were harvested by filtering through a 0.45 um filter (Sartorius, 16533) and supplemented with 8μg/ml polybrene (Millpore ...
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bioRxiv - Microbiology 2019Quote: The experimental platform is an improved version of a previous on-line flow cytometry platform [28][12] and actually comprises three modules (Figure 1): (a) a conventional culture device (Biostat B-Twin, Sartorius, 2 L), a physical interface for sampling and dilution comprising peristaltic pumps and a mixing chamber (c ...
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bioRxiv - Physiology 2019Quote: ... were inoculated with 2 mL of stock culture and grown at 30°C and at 200 rpm for 8 h in a B Braun Certomat BS-1 incubator (Sartorius, Melsungen, Germany). Bioreactor batch cultures were started by transferring 400 mL of preculture to a bioreactor containing 4.6 L of medium ...
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bioRxiv - Systems Biology 2020Quote: ... virus was grown on Vero E6 cells and concentrated using Vivaspin® 20 concentrators (Sartorius Stedim Biotech). Virus stocks were stored at −80°C ...
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bioRxiv - Molecular Biology 2022Quote: ... the virus was purified using an Adenovirus (Ad5) Purification and Concentration Kit (AdenoPACK 20; Sartorius, Goettingen, Germany) according to the manufacturer’s instructions and stored at −80 °C until use ...
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bioRxiv - Immunology 2020Quote: ... the supernatant containing virus particles was harvested and filtered with a 0.45 μm syringe filter (Sartorius Stedim Biotech). Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio ...
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bioRxiv - Microbiology 2021Quote: ... putida KT2440 was cultivated in a stirred bioreactor (Biostat B-Twin, Sartorius) in continuous mode with glucose feed pulses (0.5 g L-1 per pulse ...
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bioRxiv - Microbiology 2023Quote: ... putida KT2440 was grown in a stirred bioreactor (Biostat B-Twin, Sartorius) with a total volume of 2 L and a working volume of 1 L ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then passed through 0.22 μm filter followed by 20 nm ViroSart virus filter (Sartorius Stedim, Germany). The clarified samples were then reconstituted in formulation buffer ...
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Systems Biology 2021Quote: ... A 2 L bioreactor equipped with a Sartorius BIOSTAT B® fermentation controller (Sartorius Stedim Biotech GmbH ...
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bioRxiv - Systems Biology 2022Quote: Co-culture experiments were performed in lab-scale stirred bioreactors (Biostat B-Twin, Sartorius). The processes were performed with a working volume of 1L at a constant temperature of 30°C ...
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bioRxiv - Immunology 2022Quote: KD measurements of Fc-tagged Human FcμR (Cat: 13556-H02H, SinoBiological) immobilised on Anti-Human IgG Fc (AHC) (Cat: 18-5060, Sartorius) biosensors were performed on the Octet Red96® system with the loading threshold set at 1.0 nm and utilizing the recombinant Pertuzumab and Trastuzumab IgM VH whole antibodies variants in five serial diluted concentrations (12.5 to 200nM ...
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bioRxiv - Microbiology 2022Quote: The filtration efficiency of AF-Fe against virus-loaded aerosols was assessed using a compact experimental setup composed mainly of a polycarbonate filtration holder (Sartorius, Germany) connected to three 1.5 l compressed air tanks (PanGas ...
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bioRxiv - Immunology 2022Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb at a threshold of 0.8nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-Human Fc sensor tips (ForteBio, now Sartorius) were loaded with 200 nM mAb or FcACE2 to a threshold of 0.8 nm and then submerged into wells containing 100 nM (protomer/monomer concentration ...
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bioRxiv - Neuroscience 2023Quote: ... The recovered virus fraction was purified by first passing through a 100-kDA molecular weight cut-off (MWCO) centrifugal filter (Sartorius VIVASPIN VS2041) and then through an Amicon Ultra 2ml Centrifugal filter (Millipore UFC210024) ...
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bioRxiv - Neuroscience 2024Quote: ... The recovered virus fraction was purified by first passing through a 100 kDa molecular weight cutoff (MWCO) centrifugal filter (Sartorius, VIVASPIN VS2041) and then through an Amicon Ultra 2mL Centrifugal filter (Millipore #UFC210024) ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: ... The recovered virus fraction was purified by first passing through a 100 kDa molecular weight cutoff (MWCO) centrifugal filter (Sartorius, VIVASPIN VS2041) and then through an Amicon Ultra 2 ml Centrifugal filter (Millipore ...
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bioRxiv - Synthetic Biology 2019Quote: pH-Controlled bioreactor batch fermentations were conducted with a Biostat B+ (Sartorius Stedim, NY, USA) dual 1.5 L fermentation system at a working volume of 1 L M9 hybrid medium ...
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bioRxiv - Microbiology 2021Quote: Cultures in segregostat mode were performed in lab-scale stirred bioreactor (Biostat B-Twin, Sartorius) with 1 L working volume ...
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bioRxiv - Bioengineering 2024Quote: The three-step batch cocultivation was done by using Biostat B Plus bioreactor (Sartorius, Germany) equipped with pH-meter (Hamilton ...
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bioRxiv - Immunology 2023Quote: All BLI for data shown in Figure 1 and Figure S1 was carried out on an OctetRED384 (ForteBio) using anti-human Fc-capture sensors (Sartorius, 18-5060) to immobilize anti-PfRH5 mAbs ...
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bioRxiv - Neuroscience 2021Quote: ... The recovered virus fraction was purified by first passing through a 100-kDa molecular weight cut off (MWCO) centrifugal filter (Sartorius Vivaspin, Cat# VS2041) and then through an Amicon Ultra 2ml Centrifugal filters (Merck Millipore ...
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bioRxiv - Bioengineering 2021Quote: Bioreactor cultivation was performed as duplicates in 2 L Biostat B-DCU bioreactors (Sartorius AG, Germany) with a working volume of 1.5 L ...
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bioRxiv - Microbiology 2022Quote: ... thermolithotrophicus was continuously grown in a 10 L fermenter (BIOSTAT® B plus, Sartorius, Göttingen, Germany) under diazotrophic conditions with either 10 mM Na2SO3 or Na2SO4 as sulfur source instead of Na2S ...
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bioRxiv - Immunology 2023Quote: ... The GRAd32 S-2P vector was expanded in a 2 L Bioreactor (Biostat B DCU; Sartorius). The titer of virus contained in the bulk cell lysates was quantified by qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... Anti-human IgG Fc capture (AHC) biosensor tips (Sartorius) were equilibrated for ten minutes in a 1:4 mixture of conditioned Freestyle 293 medium and 1X HBS EP+ to match the diluted media ...
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bioRxiv - Biochemistry 2022Quote: ... protein solutions were concentrated to about 12 mg ml-1 using a centricon centrifugal filter unit (Sartorius Stedim). The protein concentration was estimated by measuring the absorbance at 280 nm.
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bioRxiv - Systems Biology 2021Quote: ... faecalis V583 ΔglnA was grown in glucose-limited chemostat cultures in Biostat B Plus benchtop bioreactors (Sartorius) in 750 ml CDM-LAB with a dilution rate of 0.15/h at 37 °C and gassing with 0.05 L/min nitrogen and stirring with 250 rpm ...
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bioRxiv - Immunology 2020Quote: ... Vectors were then expanded up to a production in 2L Bioreactor (Biostat B DCU, Sartorius Stedim Biotech), whereby suspension cells were seeded at 5e5 cells/mL ...
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bioRxiv - Neuroscience 2023Quote: In vivo: Tissue was lysed using a Mikro-Dismembrator S (B. Braun Biotech International GmbH, Sartorius group) in TRIzol (Thermo Fischer Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cell culture was inoculated at OD600 0.3 in the 2 L bioreactor (Biostat B, Sartorius, Germany) including the 1 L modified M9 minimal medium ...
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bioRxiv - Immunology 2020Quote: ... Protein A biosensors (FortéBio/Sartorius) were first hydrated for 10 minutes in Kinetics buffer ...