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Citations for Sartorius :
1 - 46 of 46 citations for Hantaan Virus Nucleoprotein HEK293 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Anti-His-tag (HIS1K) biosensors (Sartorius) were incubated in BLI buffer (0.002% v/v Tween-20 in 1× PBS ...
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bioRxiv - Immunology 2021Quote: ... S2-His-tag was immobilized on Anti-Penta-His (HIS1K) biosensors (Sartorius Corporation), and a concentration series of S2 binding mAb Fab at 200 ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-CoV-2 S2-His-tag at 10 µg/mL was loaded on Anti-Penta-HIS (HIS1K) biosensors (Sartorius Corporation) for 1 min ...
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bioRxiv - Immunology 2021Quote: RBD-His-tag at 5 µg/mL was first loaded on Ni-NTA (NTA) biosensors (Sartorius Corporation) for 15 sec and subsequently blocked with 5 µg/mL mAbs or BLI assay buffer for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Purified nucleoproteins were concentrated by ultrafiltration using appropriate VivaSpin devices (Sartorius), were snap-frozen in liquid nitrogen in small aliquots and stored at −80°C.
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bioRxiv - Plant Biology 2019Quote: ... The protein without His-Tag was concentrated using a Vivaspin 20 Ultrafiltration Unit (3 kDa molecular weight cutoff, Sartorius, www.sartorius.de). An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL ...
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Biophysics 2024Quote: ... His-tagged dROS1 protein (construct 2-3) were loaded onto penta-His sensors (Sartorius) to a response unit of about 0.5 nm followed by quenching in 10 ng/mL biocytin (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Anti-penta-HIS biosensors (Sartorius) were immersed in kinetics buffer (PBS + 0.5% BSA + 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2022Quote: ... or HIS 1K biosensors (Sartorius/ForteBio) (CrossMAb ...
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bioRxiv - Molecular Biology 2020Quote: ... The collected virus was filtered (0.45 μm filter, Sartorius) and concentrated using PEG-it overnight at 4°C (SBI Integrated Sciences ...
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bioRxiv - Microbiology 2022Quote: ... Infectious virus titer was determined using an Incucyte system (Sartorius) to quantify the red fluorescence from the mCherry reporter signal as detailed above ...
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bioRxiv - Microbiology 2021Quote: ... and virus particles were enriched in Vivaspin 20 columns (Sartorius Stedim, Biotech) via centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... the virus supernatant was concentrated using Vivaspin 20 concentrators (Sartorius Stedim Biotech) by spinning at 6000 g for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... virus was concentrated using the 100000 MWCO Vivaspin® Concentrator (Sartorius Stedim) and titrated on mammary epithelial cells ...
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bioRxiv - Immunology 2021Quote: ... His-tagged ACE2 was immobilized onto Ni-NTA coated biosensors (Sartorius) before association of increasing concentrations of each RBD for 120 seconds followed by dissociation using HBS pH 7.4 for 360 seconds ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated by centrifugation in a 100,000 MWCO ultrafiltration unit (Vivaspin, Sartorius). Titers of virus stocks were determined by plaque assay on BV2 cells(57).
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bioRxiv - Bioengineering 2022Quote: ... and was captured by anti-Penta-His (HIS1K) sensors (Sartorius, 18-5120). The primary antibody was diluted to 150nM with a running buffer in wells ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was amplified and purified using AdenoPACK 20 Adenovirus (Ad5) purification & concentration kit (Sartorius).
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bioRxiv - Developmental Biology 2020Quote: ... 10 mL of virus supernatant was filtered through a 0.45 um filter (Sartorius, 16533) and supplemented with 8µg/ml polybrene (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... the virus-containing medium was filtered through a 0.45 µm membrane filter (Sartorius®) after 2.5 days ...
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bioRxiv - Molecular Biology 2019Quote: Live cells monitoring of virus infection was performed using the IncuCyte Live-Cell Analysis system (Sartorius). Cells were seeded in a 24-well plated at 80% confluency ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested and virus particles were purified from cytokines and concentrated using Vivaspin 20 (Sartorius, filtration units with a size exclusion of 100 kDa ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 mL of virus supernatant were harvested by filtering through a 0.45 um filter (Sartorius, 16533) and supplemented with 8μg/ml polybrene (Millpore ...
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bioRxiv - Systems Biology 2020Quote: ... virus was grown on Vero E6 cells and concentrated using Vivaspin® 20 concentrators (Sartorius Stedim Biotech). Virus stocks were stored at −80°C ...
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bioRxiv - Molecular Biology 2022Quote: ... the virus was purified using an Adenovirus (Ad5) Purification and Concentration Kit (AdenoPACK 20; Sartorius, Goettingen, Germany) according to the manufacturer’s instructions and stored at −80 °C until use ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... the supernatant containing virus particles was harvested and filtered with a 0.45 μm syringe filter (Sartorius Stedim Biotech). Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... The tag-free RnpM protein was collected and concentrated using a Vivaspin turbo 15 (Sartorius, Germany).
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bioRxiv - Bioengineering 2023Quote: ... The samples were then passed through 0.22 μm filter followed by 20 nm ViroSart virus filter (Sartorius Stedim, Germany). The clarified samples were then reconstituted in formulation buffer ...
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bioRxiv - Immunology 2021Quote: His-tagged FcαRI (Sinobiological, Cat: 10414-H08H) were immobilized via Ni-NTA biosensor (Sartorius, Cat: 18-5101) and subject to free floating IgA variants to obtained ka ...
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Cell Biology 2022Quote: ... His-PKR was further concentrated to at least 10 mg/ml using Vivaspin centrifugal concentrator 30 K (Sartorius), snap-frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Microbiology 2022Quote: The filtration efficiency of AF-Fe against virus-loaded aerosols was assessed using a compact experimental setup composed mainly of a polycarbonate filtration holder (Sartorius, Germany) connected to three 1.5 l compressed air tanks (PanGas ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg/ml in Kinetics buffer (PBS 0.01% BSA) and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biophysics 2024Quote: ... The 50 ml containing 6-His-AdhE was concentrated to a final volume of 10.5 ml using a Vivaspin 20 centrifugal concentrator (Sartorius) with a molecular weight cut-off of 30 kDa and then dialysed against buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... The Hs53BP1 fragment without its GST tag was recovered in the flow through and concentrated using Vivaspin concentrator (Sartorius).
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bioRxiv - Neuroscience 2023Quote: ... The recovered virus fraction was purified by first passing through a 100-kDA molecular weight cut-off (MWCO) centrifugal filter (Sartorius VIVASPIN VS2041) and then through an Amicon Ultra 2ml Centrifugal filter (Millipore UFC210024) ...
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bioRxiv - Neuroscience 2024Quote: ... The recovered virus fraction was purified by first passing through a 100 kDa molecular weight cutoff (MWCO) centrifugal filter (Sartorius, VIVASPIN VS2041) and then through an Amicon Ultra 2mL Centrifugal filter (Millipore #UFC210024) ...
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Somatostatin interneurons control the timing of developmental desynchronization in cortical networksbioRxiv - Neuroscience 2023Quote: ... The recovered virus fraction was purified by first passing through a 100 kDa molecular weight cutoff (MWCO) centrifugal filter (Sartorius, VIVASPIN VS2041) and then through an Amicon Ultra 2 ml Centrifugal filter (Millipore ...
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bioRxiv - Immunology 2021Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 µg/ml and loaded on pre-hydrated anti-penta-HIS biosensors (Sartorius) for 2.5 min ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Immunology 2022Quote: ... His-tagged SARS-CoV-2 RBD was prepared at 8 μg ml−1 and loaded on prehydrated anti-penta-HIS biosensors (Sartorius) for 3 min ...
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bioRxiv - Neuroscience 2021Quote: ... The recovered virus fraction was purified by first passing through a 100-kDa molecular weight cut off (MWCO) centrifugal filter (Sartorius Vivaspin, Cat# VS2041) and then through an Amicon Ultra 2ml Centrifugal filters (Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... IgGs produced from the scFvs from the above sort were assessed for their competition of binding with one another using anti-Penta HIS (His1K) biosensors (Sartorius/ForteBio). His1K tips were pre-quenched with buffer containing 10nM biotin ...