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Citations for Sartorius :
1 - 50 of 58 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... His-tagged antigens using Anti-Penta-HIS biosensors (Sartorius/ForteBio). Antigen was loaded to a threshold of 1 nm shift ...
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bioRxiv - Immunology 2022Quote: ... biotinylated antigens (200 nM) were loaded onto Streptavidin biosensors (Sartorius) and then dipped into antibody solutions (200 nM ...
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bioRxiv - Synthetic Biology 2023Quote: ... IgGs were assessed for binding to biotinylated antigens using streptavidin biosensors (Sartorius/ForteBio). Antigen was loaded at a concentration of 200nM ...
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bioRxiv - Synthetic Biology 2022Quote: ... IgGs and Fabs were assessed for binding to biotinylated antigens using streptavidin (SA) biosensors (Sartorius/ForteBio) or to unbiotinylated ...
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bioRxiv - Immunology 2023Quote: ... Antigen targets were probed in serial dilutions ranging from 5000 to 15.6 nM in 1X kinetic buffer (ForteBio/Sartorius). Equilibrium dissociation constants (KD’s ...
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bioRxiv - Immunology 2023Quote: The binding affinity of recombinant antibodies to antigens was estimated by bio-layer interferometry (Octet® QK, ForteBio/Sartorius). Antibodies were loaded at 10-20 nM onto anti-human IgG Fc capture or anti-Mouse IgG Fc capture biosensors ...
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bioRxiv - Immunology 2022Quote: ... 200 nM of randomly biotinylated SARS-CoV-2 S or RBD protein antigen was captured using SA biosensors (18-5019, Sartorius). The biosensor was loaded with antigen for 5 min and then moved into the saturating mAbs at a concentration of 100 µg/mL for 10 min ...
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bioRxiv - Immunology 2022Quote: KD measurements of the Pertuzumab and Trastuzumab IgMs to their Her2 antigen (Cat: 10004-HCCH, SinoBiological) were performed by first immobilizing them on Protein L (Cat: 18-5085, Sartorius) and CaptureSelect™ Biotin Anti-IgM Conjugate (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... purified (Vivapure AdenoPACK 100, Sartorius) and titred using a TCID50 assay ...
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bioRxiv - Physiology 2023Quote: ... purified using Vivapure AdenoPACK 100 (Sartorius), and titred as described previously (21) ...
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bioRxiv - Molecular Biology 2020Quote: ... purified using the Vivapure Adenopack 100 (Sartorius) and titrated by end-point dilution in HEK293 cells.
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bioRxiv - Biochemistry 2020Quote: ... The protein was expressed and purified as previously described.26 The purified protein was concentrated to 10-20 mg/mL using a Vivaspin15R (Sartorius), and purity was estimated to be at least 95% by SDS-PAGE.
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated with VIVASPIN concentrators (Sartorius) and dialyzed against E-Buffer with 1 mM DTT ...
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bioRxiv - Microbiology 2021Quote: ... Purified NS1 was then concentrated using Vivaspin columns (Sartorius).
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using centrifugal filter devices (Vivaspin, Sartorius). Protein concentration was estimated from its absorbance at 280 nm ...
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bioRxiv - Microbiology 2020Quote: ... The purified Rel was concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 50 kDa) ...
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bioRxiv - Immunology 2021Quote: ... Purified nucleoproteins were concentrated by ultrafiltration using appropriate VivaSpin devices (Sartorius), were snap-frozen in liquid nitrogen in small aliquots and stored at −80°C.
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bioRxiv - Microbiology 2023Quote: ... The purified proteins were concentrated in a Vivaspin turbo 15 (Sartorius) centrifugal filter device (cut-off 5 or 50 kDa) ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing purified β2HA were pooled and concentrated on Vivaspin columns (Sartorius).
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bioRxiv - Biophysics 2023Quote: ... Purified nucleocapsid tubes were concentrated using Vivapore solvent absorption chambers (Sartorius, Germany) and subsequently used for further processing ...
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bioRxiv - Biochemistry 2021Quote: ... Purified Spike was concentrated using a 100 kDa cut-off concentrator (Sartorius, GE) and loaded onto a HiLoad 16/60 superdex 200 pre-equilibrated with 20 mM Hepes ...
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bioRxiv - Microbiology 2020Quote: ... concentrated and purified using Vivaspin 20 ultrafiltration units (300kDa MW cut-off, Sartorius), and stored at −80°C.
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bioRxiv - Immunology 2020Quote: ... Purified viral particles were obtained by Ion Exchange (VivaPure® AdenoPACKTM 20, Sartorius), and the DNA extracted and subjected to Next Generation Sequencing ...
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bioRxiv - Microbiology 2020Quote: ... The purified antibodies were buffer exchanged to PBS using 100 kDa VivaSpin20 columns (Sartorius). The IgG concentration was determined on the NanoDrop 2000 and the antibodies were stored at 4°C until further analyses.
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bioRxiv - Neuroscience 2020Quote: ... Water was purified using an Arium 611 Pro VF purification system (Sartorius, Göttingen, Germany) to laboratory-graded water (18.2 MΩ × cm).
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bioRxiv - Microbiology 2019Quote: ... Purified schizonts were ruptured with a 1.2 μm Ministart syringe filter (Sartorius Stedim Biotech) to release individual merozoites and 25 μl merozoite solution was added to blood obtained from mice of interest (10 ul at 100,000 cells per μl ...
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bioRxiv - Bioengineering 2022Quote: ... 25ng/ul of purified mAbs were captured on a AHC biosensor (Sartorius, 18-5060). The baseline was recorded for 60s in a running buffer (PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was amplified and purified using AdenoPACK 20 Adenovirus (Ad5) purification & concentration kit (Sartorius).
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bioRxiv - Plant Biology 2022Quote: Deionized water was purified with the Arium® purification system (Sartorius AG, Goettingen, Germany). Methanol was of LC-MS grade ...
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bioRxiv - Biochemistry 2021Quote: ... Purified enzyme was concentrated (30 kDa cutoff Amicon Stirred Cell, Millipore or VivaSpin4 columns, Sartorius). Protein concentration was determined spectrophotometrically at 280 nm (μCuvette ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing purified protein were concentrated using Vivaspin® 6 5,000 MWCO columns (Sartorius Stedim). Protein was buffer exchanged using membrane filtration into 50 mM NaP ...
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bioRxiv - Immunology 2022Quote: ... Purified protein was buffer exchanged into PBS and concentrated using a Vivaspin concentrator (Sartorius-Stedim) to 3-5 mg/mL and flash-frozen with liquid nitrogen and stored at −80 °C.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... was purified using centrifugal filtration (cut-off 30 kDa, Vivaspin 500, Sartorius AG, Goettingen, Germany) eight times for 2 min at 15’000 × g at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Purified His6-MBP-tagged proteins were then concentrated with a 30,000 MWCO centrifugal concentrator (Sartorius) and buffer exchanged into 20 mM HEPES pH 7.5 and 50 mM NaCl for crystallization ...
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bioRxiv - Cell Biology 2023Quote: Low molecular weight alginate (L3, Kimica) was purified using a tangential flow filtration system (Sartorius) and subsequently coupled with GGGRGDSP peptide (Biomatik ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested and virus particles were purified from cytokines and concentrated using Vivaspin 20 (Sartorius, filtration units with a size exclusion of 100 kDa ...
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bioRxiv - Microbiology 2020Quote: ... The purified protein was buffer exchanged into PBS using a Vivacon 500 concentrator (Cat: VS0122, Sartorius Stedim). For the generation of human ACE2-hFc and SARS-CoV-2 RBD-mFc recombinant proteins ...
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bioRxiv - Microbiology 2021Quote: ... the purified proteins were run on Superdex S200 size-exclusion column and concentrated with Vivaspin devices (Sartorius).
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bioRxiv - Molecular Biology 2022Quote: ... the virus was purified using an Adenovirus (Ad5) Purification and Concentration Kit (AdenoPACK 20; Sartorius, Goettingen, Germany) according to the manufacturer’s instructions and stored at −80 °C until use ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The water used for HPLC analysis and extraction was purified by ultrapure water system arium pro (Sartorius).
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bioRxiv - Bioengineering 2020Quote: ... The AAV2/8 was purified by affinity chromatography and concentrated using ultrafiltration columns (Sartorius Stedim Biotech, Goettingen, Germany), washed in PBS and concentrated to a volume of 100–150 µl ...
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bioRxiv - Biochemistry 2022Quote: ... Purified yCAF-1 complexes (Figure 1- figure supplement 1a) were concentrated with 100 000 MWCO centrifugal concentrators (Sartorius), aliquoted in small volumes ...
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bioRxiv - Microbiology 2023Quote: ... Purified protein fractions were dialyzed against 1X PBS (pH 7.4) using the Vivaspin 10 kDa centrifuge system (Sartorius). Protein quantification was performed in a spectrophotometer at 280 nm and the assays were performed with the enzyme at a concentration of 5.88 μM.
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated toxins were purified using buffer exchange columns (Vivacon 500, Sartorius, 3000 Da Molecular Weight Cut-Off) using the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Vesicles were purified from 1 L of culture per replicate using a tangential flow filtration system (Vivaflow 200, Sartorius). Details on all methods ...
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bioRxiv - Biochemistry 2020Quote: ... the purified NT-mini/NT*-mini was concentrated to 1 mg/mL with a Vivaspin Turbo10 kDa MWCO concentrator (Sartorius), flash frozen in liquid nitrogen and stored at −80 °C ...
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bioRxiv - Immunology 2021Quote: ... Purified S proteins were exchanged to storage buffer and then filtered through 0.22 um filter (Sartorius Stedim Biotech, Göttingen, Germany). The concentration of purified protein was determined by ELISA ...
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bioRxiv - Biochemistry 2022Quote: The specificity of the binding consisted in the immobilization of 2 μg/mL of purified VHHs on Anti-His coated sensors (His1K, Sartorius) via their His6 tag ...
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bioRxiv - Biochemistry 2024Quote: Affinity of recombinant RBDs towards the purified ectodomain of TMPRSS2S441Awas assessed in real-time using a bio-layer interferometry Octet-R8 device (Sartorius). Nickel-NTA capture sensors (Sartorius ...