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Citations for Sartorius :
1 - 50 of 149 citations for Adenovirus Type 5 Particles CMV Luciferase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Virus was amplified and purified using AdenoPACK 20 Adenovirus (Ad5) purification & concentration kit (Sartorius).
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bioRxiv - Molecular Biology 2022Quote: ... the virus was purified using an Adenovirus (Ad5) Purification and Concentration Kit (AdenoPACK 20; Sartorius, Goettingen, Germany) according to the manufacturer’s instructions and stored at −80 °C until use ...
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bioRxiv - Microbiology 2019Quote: ... cells were stained with SYBR Green I (Marie et al., 1997) and large suspended particles were removed by filtration through 5 µm pore-size filters (Sartorius) to avoid clogging of the flow cytometer ...
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bioRxiv - Cell Biology 2022Quote: ... Particles were filtrated with 0.45µm pore size filter (Sartorius). HT22-CRHR1 cells were incubated 48 h with filtrated lentiviral particles of specific shRNAs for knockdown and a control (MISSION pLKO.1-puro Non-Target shRNA plasmid) ...
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bioRxiv - Cancer Biology 2023Quote: ... The UGT2B15 promoter 20 base-pair sequence (5’-TAACTTGATTGATTTTTCCT-3’ for wild type and 5’-TAACTTGGCTGTCTTTTCCT-3’ for mutant) was immobilized to a streptavidin SADH sensor chip (Sartorius) in running buffer (10 mM HEPES pH 6.8 ...
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bioRxiv - Microbiology 2021Quote: ... and virus particles were enriched in Vivaspin 20 columns (Sartorius Stedim, Biotech) via centrifugation ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell media containing lentiviral particles were concentrated using centricon column (Vivaspin 20, Sartorius). Each viral production was titrated beforehand to establish a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2020Quote: ... Purified viral particles were obtained by Ion Exchange (VivaPure® AdenoPACKTM 20, Sartorius), and the DNA extracted and subjected to Next Generation Sequencing ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested and virus particles were purified from cytokines and concentrated using Vivaspin 20 (Sartorius, filtration units with a size exclusion of 100 kDa ...
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bioRxiv - Immunology 2021Quote: ... The TFF retentate was next enriched for AAV particles on a cation exchange chromatography column (BIA Separations, Sartorius). The eluate was concentrated ...
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bioRxiv - Immunology 2020Quote: ... the supernatant containing virus particles was harvested and filtered with a 0.45 μm syringe filter (Sartorius Stedim Biotech). Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio ...
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bioRxiv - Cell Biology 2022Quote: ... viral particles were isolated by filtering the medium containing them through a 0.45μm filter (16537 from Sartorius stedim biotech). Then ...
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bioRxiv - Immunology 2019Quote: ... Cell culture medium containing lentiviral particles (LVP) was collected 48 h later and passed through a 0.45 μm filter (Sartorius). Virus preparations were then concentrated by centrifugation at 30,000 rpm for 90 min ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant containing lentiviral particles was collected after 48 h and filtered through a 0.45 μm sterile filter (Sartorius Stedim). Lentivirus supernatants were concentrated with PEG-it™ (SBI ...
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bioRxiv - Microbiology 2021Quote: ... 10E7 secreted infectious particles per sample were used) were concentrated using Vivaspin units with 100 MWA cut-of (Sartorius) by centrifugation at 3 000 g for 20 min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Cell culture medium containing lentiviral particles (LVP) was collected 48 h later and passed through a 0.45 µm filter (Sartorius). Virus preparations were then concentrated by centrifugation at 30,000 rpm for 90 min ...
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Paleontology 2019Quote: ... The resulting gelatin was then filtered using precleaned Ezee filters (Elkay Labs UK) to remove larger particles and then ultrafiltered (precleaned Sartorius Vivapsin Turbo 15) to separate large (>30 kD ...
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bioRxiv - Immunology 2022Quote: ... and aged wild-type mice were isolated and labeled using Incucyte Caspase3/7 Red Reagent (Sartorius). Cells were stimulated with 5 μg/mL plate-bound αCD3 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... The suspensions were then filtered through 0.45 μm cellulose acetate filters (type Minisart, Sartorius, Göttingen, Germany) to remove a large portion of remaining sponge cells and nuclei (microscopic visualizations showed a lack of sponge nuclei after filtration) ...
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bioRxiv - Biochemistry 2020Quote: ... Two 5 L bioreactors (Sartorius) were seeded at 0.3 x 106 cells/mL in 3 L (total working volume ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the total fresh weight of workers was measured to the nearest 0.1 mg (Sartorius, Type 1712, Göttingen, Germany) to allow for calculation of mass specific metabolic rate ...
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bioRxiv - Bioengineering 2020Quote: ... GO16 KS3 was cultivated in a 5 L stirred tank bioreactor (Sartorius UniVessel Glass 5 l) containing 3 L of MSMlim broth ...
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bioRxiv - Systems Biology 2022Quote: ... The culture pH was kept at 5.0 by automatic addition of 0.5 mol.l-1 KOH (BioStat controller, type 8843415, Sartorius AG, Germany).
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bioRxiv - Biochemistry 2020Quote: ... or Vivaspin (Sartorius, MWCO 5 kDa) concentration columns.
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bioRxiv - Plant Biology 2021Quote: ... and 5-μm syringe filter (Sartorius, Germany) to remove RLX (0 ...
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bioRxiv - Biochemistry 2021Quote: ... and 5 mM MgCl2 on BLItz machine (FortéBio Sartorius). Streptavidin coated biosensors were used in all the measurements and were hydrated in the reaction buffer for 10 minutes before loading ...
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bioRxiv - Biochemistry 2022Quote: ... 5 % CO2 in an Incucyte® Zoom (Sartorius AG) and scanned every 8 h for 2 days ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml using a Vivaspin concentrator spin column (Sartorius). The protein sample was applied to a HiLoad 16/600 Superdex 200 column (GE healthcare ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml using a Vivaspin concentrator spin column (Sartorius) at 4,500 × g ...
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bioRxiv - Biochemistry 2020Quote: ... The 5 kDa MWCO concentrator filtrate (Sartorius, Göttingen, Germany) was used to control for the amount of unbound metal in the protein samples ...
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bioRxiv - Microbiology 2023Quote: ... concentrated using Vivaspin Turbo 5 kDa centrifugal concentrators (Sartorius). Initial crystallization hits were obtained using our in-house crystallization facility (37) ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM DTT) using a 5-KD concentrator (Sartorius). The purified samples were aliquoted ...
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bioRxiv - Bioengineering 2024Quote: ... The purified protein from elutions containing the fewest contaminating proteins (typically elution steps 3-5) were concentrated in 1× PBS using a 5 kDa cut-off Vivaspin Centrifugal Concentrator (Sartorius, Oakville, Ontario, Canada). Protein concentrations were determined using the micro-BCA assay (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... ICG powder was weighed and dissolved in cell culture medium (type of medium dependent on experiment) and sterile filtered (Sartorius Minisart High Flow, PES 0.22μm) to achieve a 100μM stock solution ...
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bioRxiv - Neuroscience 2023Quote: ... then concentrated using a 5 kDa spin column (Sartorius, VSO611) and flash-frozen with liquid nitrogen in single-use aliquots for storage at -80 °C ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were concentrated by ultrafiltration (5 kDa Vivaspin, Sartorius) and further purified by reverse phase chromatography using a preparative C4 column (Vydac 214 TP ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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bioRxiv - Molecular Biology 2022Quote: ... The sample was concentrated down to 5 ml volume (Vivaspin, Sartorius) and further purified through a HiLoad 26/600 Superdex 200 pg (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was concentrated using a Viaspin column (Sartorius 5 kDas). Protein concentrations were estimated and normalized to 200 ng/μl by performing a Bradford assay.
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bioRxiv - Biochemistry 2024Quote: ... concentrated using a Vivaspin Turbo Ultrafiltration unit (5 kDa MWCO; Sartorius) and buffer-exchanged to buffer L_A by a HiPrep 26/10 desalting column (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... 5 mL of cultures were sampled and filtered through PTFE membrane (Sartorius) at time points before and after 0.5 mg/mL arginine hydroxamate treatment ...
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bioRxiv - Molecular Biology 2023Quote: ... Plates were analyzed 5 days post seeding using an Incucyte S3 (Sartorius). Additionally ...
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bioRxiv - Microbiology 2024Quote: Infected MRC-5 or ARPE19 cells were place into Incucyte S3 (Sartorius) at 2 hpi ...
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bioRxiv - Biochemistry 2021Quote: ... concentrated ~5-fold using a 10 kDa Sartocon Slice ECO Hydrosart cassette (Sartorius), and diafiltered against 4 volumes of 10 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... 3 L of water were pre-filtered through the 5 μm filter (Sartorius). The flow-through was further filtered using the 0.2 μm filter (Sterivex) ...
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bioRxiv - Immunology 2020Quote: ... concentrated to 1-5 mg/ml by ultrafiltration using appropriate VivaSpin devices (Sartorius), were snap-frozen in liquid nitrogen in small aliquots and stored at -80°C.
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bioRxiv - Immunology 2021Quote: ... Fractions were desalted and concentrated with 5-kDa cut-off Vivaspin centrifugal filters (Sartorius). Fractionated proteins were separated in 1-D SDS-PAGE in 4-12% Bis-Tris gels ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 mM of IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius, cat #4440) as well as 1:500 of Nuclight Rapid Red Dye (Sartorius ...