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Citations for Sartorius :
1 - 50 of 183 citations for Adenovirus Type 5 Particles CMV GFP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Virus was amplified and purified using AdenoPACK 20 Adenovirus (Ad5) purification & concentration kit (Sartorius).
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bioRxiv - Molecular Biology 2022Quote: ... the virus was purified using an Adenovirus (Ad5) Purification and Concentration Kit (AdenoPACK 20; Sartorius, Goettingen, Germany) according to the manufacturer’s instructions and stored at −80 °C until use ...
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bioRxiv - Microbiology 2019Quote: ... cells were stained with SYBR Green I (Marie et al., 1997) and large suspended particles were removed by filtration through 5 µm pore-size filters (Sartorius) to avoid clogging of the flow cytometer ...
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bioRxiv - Cell Biology 2022Quote: ... Particles were filtrated with 0.45µm pore size filter (Sartorius). HT22-CRHR1 cells were incubated 48 h with filtrated lentiviral particles of specific shRNAs for knockdown and a control (MISSION pLKO.1-puro Non-Target shRNA plasmid) ...
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bioRxiv - Cancer Biology 2023Quote: ... The UGT2B15 promoter 20 base-pair sequence (5’-TAACTTGATTGATTTTTCCT-3’ for wild type and 5’-TAACTTGGCTGTCTTTTCCT-3’ for mutant) was immobilized to a streptavidin SADH sensor chip (Sartorius) in running buffer (10 mM HEPES pH 6.8 ...
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bioRxiv - Microbiology 2021Quote: ... and virus particles were enriched in Vivaspin 20 columns (Sartorius Stedim, Biotech) via centrifugation ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell media containing lentiviral particles were concentrated using centricon column (Vivaspin 20, Sartorius). Each viral production was titrated beforehand to establish a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2020Quote: ... Purified viral particles were obtained by Ion Exchange (VivaPure® AdenoPACKTM 20, Sartorius), and the DNA extracted and subjected to Next Generation Sequencing ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested and virus particles were purified from cytokines and concentrated using Vivaspin 20 (Sartorius, filtration units with a size exclusion of 100 kDa ...
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bioRxiv - Microbiology 2024Quote: U2OS-53PB1-GFP and U2OS-NBS1-GFP cells were imaged using the IncuCyte S3 Live-Cell Analysis Instrument (Sartorius). Mean Fluorescence Intensity (MFI ...
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bioRxiv - Immunology 2021Quote: ... The TFF retentate was next enriched for AAV particles on a cation exchange chromatography column (BIA Separations, Sartorius). The eluate was concentrated ...
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bioRxiv - Immunology 2020Quote: ... the supernatant containing virus particles was harvested and filtered with a 0.45 μm syringe filter (Sartorius Stedim Biotech). Filtered viral supernatants were concentrated 100 times using Lenti-X™ Concentrator (Takara Bio ...
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bioRxiv - Cell Biology 2022Quote: ... viral particles were isolated by filtering the medium containing them through a 0.45μm filter (16537 from Sartorius stedim biotech). Then ...
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bioRxiv - Immunology 2019Quote: ... Cell culture medium containing lentiviral particles (LVP) was collected 48 h later and passed through a 0.45 μm filter (Sartorius). Virus preparations were then concentrated by centrifugation at 30,000 rpm for 90 min ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant containing lentiviral particles was collected after 48 h and filtered through a 0.45 μm sterile filter (Sartorius Stedim). Lentivirus supernatants were concentrated with PEG-it™ (SBI ...
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bioRxiv - Microbiology 2021Quote: ... 10E7 secreted infectious particles per sample were used) were concentrated using Vivaspin units with 100 MWA cut-of (Sartorius) by centrifugation at 3 000 g for 20 min at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Cell culture medium containing lentiviral particles (LVP) was collected 48 h later and passed through a 0.45 µm filter (Sartorius). Virus preparations were then concentrated by centrifugation at 30,000 rpm for 90 min ...
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bioRxiv - Immunology 2023Quote: ... pseudotyped SARS-CoV-2 virus particles were harvested and filtered over a 0.45 µm nitrocellulose membrane (Sartorius Stedim, Gottingen, Germany). SARS-CoV-2 pseudovirus productions were quantified by RETRO-TEK HIV-1 p24 ELISA according to manufacturer instructions (ZeptoMetrix Corporation).
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bioRxiv - Immunology 2024Quote: ... GFP expression was measured by using Incucyte S3 (Sartorius, Goettingen, Germany).
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bioRxiv - Neuroscience 2024Quote: ... Images showing synapsin-GFP alone were collected from an IncuCyte S3 (Sartorius). Images showing PI and nuclear stains were collected using a Nikon BioStation (Nikon) ...
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bioRxiv - Paleontology 2019Quote: ... The resulting gelatin was then filtered using precleaned Ezee filters (Elkay Labs UK) to remove larger particles and then ultrafiltered (precleaned Sartorius Vivapsin Turbo 15) to separate large (>30 kD ...
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bioRxiv - Immunology 2022Quote: ... and aged wild-type mice were isolated and labeled using Incucyte Caspase3/7 Red Reagent (Sartorius). Cells were stimulated with 5 μg/mL plate-bound αCD3 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... The suspensions were then filtered through 0.45 μm cellulose acetate filters (type Minisart, Sartorius, Göttingen, Germany) to remove a large portion of remaining sponge cells and nuclei (microscopic visualizations showed a lack of sponge nuclei after filtration) ...
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bioRxiv - Cancer Biology 2022Quote: ... RFP (625-705nm) and GFP (544nm) fluorescences were acquired with Incucyte system (Sartorius) each hour over 8 days.
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bioRxiv - Biochemistry 2020Quote: ... Two 5 L bioreactors (Sartorius) were seeded at 0.3 x 106 cells/mL in 3 L (total working volume ...
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bioRxiv - Immunology 2021Quote: ... Infection was measured by GFP expression using an Incucyte Live cell imaging system (Sartorius). Infection was enumerated as GFP positive cell area.
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bioRxiv - Evolutionary Biology 2023Quote: ... the total fresh weight of workers was measured to the nearest 0.1 mg (Sartorius, Type 1712, Göttingen, Germany) to allow for calculation of mass specific metabolic rate ...
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bioRxiv - Microbiology 2023Quote: ... and % GFP positive cells were enumerated by flow cytometry (Intellicyt iQue Screener PLUS, Sartorius AG). Infectious titers were determined from the most linear portions of the dose-response infectivity curves using the following formula ...
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bioRxiv - Bioengineering 2020Quote: ... GO16 KS3 was cultivated in a 5 L stirred tank bioreactor (Sartorius UniVessel Glass 5 l) containing 3 L of MSMlim broth ...
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bioRxiv - Systems Biology 2022Quote: ... The culture pH was kept at 5.0 by automatic addition of 0.5 mol.l-1 KOH (BioStat controller, type 8843415, Sartorius AG, Germany).
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bioRxiv - Biochemistry 2020Quote: ... or Vivaspin (Sartorius, MWCO 5 kDa) concentration columns.
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bioRxiv - Microbiology 2019Quote: ... and GFP positive cells quantified by flow cytometry (Intellicyt iQue Screener PLUS, Sartorius AG, Gottingen, Germany).
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: ... Images of GFP-positive cells were obtained with Incucyte® S3 Live-Cell Analysis software (Sartorius).
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bioRxiv - Microbiology 2023Quote: ... and GFP positive cells quantified by flow cytometry (Intellicyt iQue Screener PLUS, Sartorius AG, Gottingen, Germany).
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bioRxiv - Evolutionary Biology 2024Quote: ... followed by quantification of GFP-expressing infected cells using a live cell microscope (Incucyte SX5; Sartorius) to obtain the number of focus forming units (FFU ...
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bioRxiv - Plant Biology 2021Quote: ... and 5-μm syringe filter (Sartorius, Germany) to remove RLX (0 ...
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bioRxiv - Immunology 2021Quote: ... Infections were monitored in live cells via GFP expression using the Incucyte live-cell analysis system (Sartorius) for 24-72h ...
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bioRxiv - Neuroscience 2020Quote: ... Infection was measured through GFP expression measured by visualisation on an Incucyte Live cell imaging system (Sartorius). Infection was enumerated as GFP positive cell area.
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bioRxiv - Microbiology 2020Quote: ... Infection was measured through GFP expression measured by visualisation on an Incucyte Live cell imaging system (Sartorius). Infection was enumerated as GFP positive cell area ...
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bioRxiv - Microbiology 2023Quote: ... GFP fluorescence at 24 and 48 hours post transfection was analysed via the Incucyte S3 machine (Sartorius).
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bioRxiv - Cell Biology 2023Quote: Long-term fluorescence imaging of iPSC-CMs expressing NLS-GFP imaging was performed on an IncuCyte (Sartorius) incubator imaging system ...
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bioRxiv - Cancer Biology 2023Quote: ... The changes in GFP of cells in 12-well plates were monitored by using IncuCyte SX5 (Sartorius) for 72 hrs.
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bioRxiv - Evolutionary Biology 2024Quote: ... The GFP signal in each well was then determined using a live-cell microscope (Incucyte SX5, Sartorius). The average GFP signal observed in mock-infected wells was subtracted from all infected wells ...
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bioRxiv - Immunology 2020Quote: ... apoptosis of the PC3 GFP cell line was assessed using the annexin V red (cat n°4641, Sartorius). Culture was monitored every hour during 16 hours by Incucyte and overlapping of GFP (green ...
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bioRxiv - Immunology 2020Quote: ... GFP fluorescent intensity was detected every 2 hours using the IncuCyte S3 Live-Cell analysis system (Sartorius, Germany). Total integrated GFP intensity per well was used as a quantitative measure of viable target cells ...
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bioRxiv - Cancer Biology 2023Quote: Confluency analysis of GBM1 UbC-GFP cells was performed using the ZOOM Incucyte Live-Cell imaging platform (Sartorius). 8K cells per square centimetre per well were plated in 6-well plates and whole-well imaging was performed in 120-minute intervals using a Nikon 4x objective ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We obtained the GFP Integrated Intensity (GCU·µm²/image) in each condition using a live-cell imaging platform (Incucyte SX5, Sartorius) at 24 hours post mixing and standardized to the signal obtained from the positive control (Wuhan-Hu-1 spike protein) ...
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bioRxiv - Biochemistry 2021Quote: ... and 5 mM MgCl2 on BLItz machine (FortéBio Sartorius). Streptavidin coated biosensors were used in all the measurements and were hydrated in the reaction buffer for 10 minutes before loading ...
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bioRxiv - Biochemistry 2022Quote: ... 5 % CO2 in an Incucyte® Zoom (Sartorius AG) and scanned every 8 h for 2 days ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml using a Vivaspin concentrator spin column (Sartorius). The protein sample was applied to a HiLoad 16/600 Superdex 200 column (GE healthcare ...