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Citations for Sartorius :
1 - 50 of 60 citations for 6 Phthalazinecarbonylchloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... We imaged cells every 6 hours for 4-6 days of treatment using Incucyte Zoom (Sartorius, Michigan, USA). Fluorescence was quantified using four locations in each treated sample by the IncuCyte Zoom 2016B software.
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bioRxiv - Biophysics 2020Quote: ... Peak fractions containing purified Cx46/50 were pooled and concentrated to 5–6 mg mL-1 with a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius). Protein concentration was determined by UV absorbance at 280 nm ...
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bioRxiv - Microbiology 2023Quote: ... After concentration (Vivaspin 6 Concentrator, Sartorius Stedim Biotech) and desalting (PD 10 Desalting Columns ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with a Vivaspin 6 (10 kDa MWCO) (Sartorius), flash-frozen by liquid nitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The eluent was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluent was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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bioRxiv - Microbiology 2020Quote: ... using the Vivaspin 6 centrifugal concentrator (MWCO 1,000,000 kDa) (Sartorius, Australia), was required for high-quality electron microscopy visualization ...
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bioRxiv - Microbiology 2020Quote: ... and then concentrated using Vivaspin® 6 polyethersulfone 10 kDa (Sartorius). The purified and concentrated protein was delivered to GeneScript-Nanjing to raise the anti-rabbit polyclonal antibody.
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bioRxiv - Microbiology 2020Quote: ... and then concentrated using Vivaspin® 6 polyethersulfone 10 kDa (Sartorius). Concentrations of the purified proteins were measured using BCA protein assay kit (Beyotime ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluted complex was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius), and imidazole was removed from the protein solution by applying the sample to a PD MiniTrap Sample Column (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: ... concentrated to 300-350 µl using Vivaspin 6 30,000 MWCO concentrators (Sartorius), and loaded onto a Superdex 200 10/300 GL column equilibrated in SEC Buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2022Quote: ... then concentrated using Vivaspin® 6 concentrators (Sartorius Stedim Lab Ltd, UK) and stored at -80°C before use.
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bioRxiv - Plant Biology 2022Quote: ... then concentrated using Vivaspin® 6 concentrators (Sartorius Stedim Lab Ltd, UK) and stored at -80 °C before use.
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bioRxiv - Biochemistry 2023Quote: ... Dialysed protein was spin-concentrated in a Vivaspin 6 MWCO 50,000 (Sartorius) to the desired concentration ...
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bioRxiv - Systems Biology 2023Quote: ... followed by live-cell imaging every 6 h using Incucyte Systems (Sartorius) for the duration of 1 week ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsamples were weighed (20 mg ± 6 mg) on a fine scale balance (Entris, Sartorius). Together with 6 negative controls (200 μl lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... the samples were concentrated using filter columns (Vivaspin 6, 100,000 MWCO PES, Sartorius, Germany) at 4 °C with a maximal speed of 4,000 x g and washed twice with DBCM2 salt solution ...
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bioRxiv - Microbiology 2020Quote: ... The resulting supernatant was then concentrated using Vivaspin® 6 100,000 MWCO concentrator columns (Sartorius). The sample was then purified via size exclusion chromatography using a Superose™ 6 Increase column (GE Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... The aliquots containing the purified His-ChuP were concentrated using a VivaSpin 6 column (Sartorius), and desalted by gel filtration in PD-10 column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Samples containing purified protein were concentrated using Vivaspin® 6 5,000 MWCO columns (Sartorius Stedim). Protein was buffer exchanged using membrane filtration into 50 mM NaP ...
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bioRxiv - Immunology 2022Quote: ... and the eluate was collected into a Vivaspin 6 50 kDa MWCO column (Sartorius, V10631), which was centrifugated at 2,113 g for five minutes to concentrate the antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... the volume was adjusted to 5 mL and transferred to a Centrifugal Concentrator (Vivaspin 6; Sartorius). The retentate was adjusted to 120 µL prior to analysis.
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bioRxiv - Biophysics 2020Quote: ... were collected and concentrated using a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius) to a final concentration ∼2.5 mg mL-1 ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing Ldh-EtfAB were pooled and concentrated via ultrafiltration (Vivaspin 6, 30 kDa Cut-off, Sartorius Stedim Biotech GmbH ...
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bioRxiv - Microbiology 2023Quote: ... which took place overnight on arrival at 4°C (cold room) using 6 Vivaflow200 filtration units (Sartorius) operating in two parallel series ...
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bioRxiv - Microbiology 2024Quote: ... 10 % glycerol) and proteins were concentrated using vivaspin 6 columns with a 5000 Da cut off (Sartorius). To remove the His-tag ...
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bioRxiv - Systems Biology 2022Quote: ... and then 6 hours after doxorubicin treatment was moved to an incubator connected to an Incucyte microscope (Sartorius). Brightfield images of wells were taken 6 ...
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bioRxiv - Cell Biology 2022Quote: ... 6 ml of the crude CM was passed through a 300 kDa Vivaspin concentrator (Sartorius AG, Göttingen, Germany) by centrifugation at 3,000 g for ∼15 minutes (or until the retentate was ∼300 µl) ...
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bioRxiv - Microbiology 2020Quote: ... Eluted proteins were concentrated using an ultrafiltration membrane with a 10 kDa molecular weight cutoff (Vivaspin 6, Sartorius). To reconstitute the BAM-DolP complex in vitro ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6.0) using a centrifugal concentrator with a molecular weight cutoff of 10 kDa (Vivaspin 6, VS0602, Sartorius). The presence of the fucosidase (62 kDa ...
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bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
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bioRxiv - Biochemistry 2022Quote: ... and buffer was exchanged four times with 5 mL of 10 mM potassium phosphate buffer pH 7.4 using a Vivaspin 6 Centrifugal Concentrator (Sartorius) at 2,000 rcf and 4°C for 20 min ...
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bioRxiv - Biochemistry 2021Quote: ... Purified V-ATPase in buffer containing LMNG was concentrated to 1.9 mg/mL with a 100 kDa MWCO VivaSpin 6 centrifugal concentrator at 2000 ×g for 15 min (Sartorius), followed by a VivaSpin 500 concentrator at 12,000 ×g for 2 min.
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bioRxiv - Biophysics 2023Quote: ... Fractions containing Ub were concentrated and buffer exchanged into pH 6.8 buffer (see above) using Vivaspin 6 centrifugal concentrators with a molecular weight cutoff (MWCO) of 5000 Da (Sartorius). Purified proteins were frozen at −80°C.
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bioRxiv - Microbiology 2023Quote: ... whole well images were taken at 6 h intervals using an Incucyte Zoom S3 live cell imaging system (Sartorius).
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bioRxiv - Microbiology 2023Quote: ... Total green cell count was measured every 6 h using an Incucyte Zoom S3 live cell imaging system (Sartorius) and normalised for confluency variation ...
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was then concentrated to the approximate concentration of 10 mg/ml using a Vivaspin 6 3,000 MWCO PES (Sartorius) membrane ultrafiltration tube.
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bioRxiv - Bioengineering 2024Quote: ... and arginine (pH 6) to a final concentration of ≥200 mg/mL using the Ambr Crossflow system (Sartorius, Germany).
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bioRxiv - Biophysics 2024Quote: ... The 50 ml containing 6-His-AdhE was concentrated to a final volume of 10.5 ml using a Vivaspin 20 centrifugal concentrator (Sartorius) with a molecular weight cut-off of 30 kDa and then dialysed against buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions were checked via SDS-PAGE and the cleanest Chz1-containing fraction was concentrated using a 10 kDa cutoff Vivaspin 6 concentrator (Sartorius) at 3,000 g at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The nuclear suspension was centrifugated for 30 min at 15 000 g and supernatant was concentrated using Vivaspin 6 (10 kDa MWCO) centrifugal concentrator (Sartorius). Aliquots of concentrated nuclear extracts were frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Systems Biology 2020Quote: 2 mL of collected spent media was loaded in Vivaspin 6™ Centrifugal Concentrator 10,000 MWCO PES (Sartorius AG, Germany) and centrifuged at 4000 RPM for 13 minutes to concentrate samples ...
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bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and cells were imaged live every 6 hours using the IncuCyte® ZOO system for automated live cell analysis (Sartorius). Wound closure was analysed by measuring the spatial cell density in the wound area relative to the spatial cell density outside of the wound expressed as the Relative Wound Density (% ...
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bioRxiv - Biophysics 2023Quote: Nanodisc samples were concentrated to ∼200 μM in 280 μL in a Vivaspin-6 concentrator with a 30 kDa MWCO (Sartorius). 20 μL D2O was added and gently mixed into the sample ...
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bioRxiv - Biochemistry 2023Quote: ... The flow through was collected and concentrated to 6-10 mg/ml using a 100 kDa molecular weight (MW) cutoff centrifugal concentrator (Sartorius) and loaded onto a Superdex 200 Increase 10/300 GL column equilibrated with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Neuroscience 2023Quote: ... Eluent from the first two elution fractions after the elimination of dead volume were concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Eluent from the first two elution fractions after the elimination of dead volume were concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... the fractions obtained by affinity chromatography were pooled and concentrated using conical membrane ultrafiltration tubes (Vivaspin 6 mL 10 kDa MWCO, Sartorius) to 7 mg/mL (2 mL) ...