Labshake search
Citations for Sartorius :
1 - 50 of 369 citations for 6 METHOXY 1 3 BENZODIOXOL 5 AMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
-
bioRxiv - Biochemistry 2022Quote: ... amine-reactive second-generation (AR2G) biosensor (Sartorius) tips were activated in 20 mM 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC ...
-
bioRxiv - Microbiology 2022Quote: ... Amine Reactive Second Generation (AR2G) biosensors (Sartorius) were hydrated in water ...
-
bioRxiv - Biophysics 2020Quote: ... Peak fractions containing purified Cx46/50 were pooled and concentrated to 5–6 mg mL-1 with a centrifugal device (Vivaspin 6; 50-kDa cut-off filter; Sartorius). Protein concentration was determined by UV absorbance at 280 nm ...
-
bioRxiv - Biochemistry 2024Quote: ... was immobilized on Octet Amine Reactive 2nd Generation (AR2G) Biosensors (Sartorius) at a concentration of 200 nM in a 10 mM sodium acetate buffer (pH 5.0 ...
-
bioRxiv - Bioengineering 2024Quote: ... The purified protein from elutions containing the fewest contaminating proteins (typically elution steps 3-5) were concentrated in 1× PBS using a 5 kDa cut-off Vivaspin Centrifugal Concentrator (Sartorius, Oakville, Ontario, Canada). Protein concentrations were determined using the micro-BCA assay (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: ... The pooled fractions of the NOTCH3 EGF1-5 WT and R133C were concentrated to 1 mg/mL in a concentration column (Vivaspin 6 10 KDa MWCO, Sartorius) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... The immobilization of ligand proteins on amine reactive second-generation (AR2G) biosensors (Sartorius) was done following manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2023Quote: ... with a CIM quaternary amine (QA) 8 mL column (Sartorius BIA separations, Slovenia), and with step gradient elution to a final elution volume of 25 mL ...
-
bioRxiv - Biochemistry 2023Quote: ... STEAP1 was immobilized on amine reactive second-generation (AR2G) biosensors (Sartorius, Göttingen, Germany). The biosensor tips were activated for 5 min in EDC and 10 mM Sulfo-NHS before being loaded with STEAP1 at a concentration of 1 µg/mL for 10 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... The UGT2B15 promoter 20 base-pair sequence (5’-TAACTTGATTGATTTTTCCT-3’ for wild type and 5’-TAACTTGGCTGTCTTTTCCT-3’ for mutant) was immobilized to a streptavidin SADH sensor chip (Sartorius) in running buffer (10 mM HEPES pH 6.8 ...
-
bioRxiv - Microbiology 2021Quote: ... 3 L of water were pre-filtered through the 5 μm filter (Sartorius). The flow-through was further filtered using the 0.2 μm filter (Sterivex) ...
-
bioRxiv - Molecular Biology 2021Quote: ... the volume was adjusted to 5 mL and transferred to a Centrifugal Concentrator (Vivaspin 6; Sartorius). The retentate was adjusted to 120 µL prior to analysis.
-
bioRxiv - Cancer Biology 2021Quote: ... 5 mM of IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius, cat #4440) as well as 1:500 of Nuclight Rapid Red Dye (Sartorius ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 5 μM Caspase 3/7 dye was included in the media (Sartorius #4704). The number of red nuclei (indicating active caspase 3/7 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Caspase 3/7 dye (4440, Sartorius, 1/1000) and/or propidium Iodide (PI ...
-
bioRxiv - Microbiology 2023Quote: ... The proteins were spin concentrated to 5 mg / ml (3 kDa MWCO, vivaspin 20, Sartorius), aliquoted ...
-
bioRxiv - Immunology 2024Quote: ... Plates were imaged every 4-6 hours for 3-7 days using the IncuCyte® Live-Cell Analysis System (Sartorius). 5 images per well at 10x zoom were collected at each time point ...
-
bioRxiv - Bioengineering 2024Quote: ... After 24 hrs supernatant was collected and concentrated by the Vivaspin-6 5 kDa MWCO filters (Sartorius VS06S1). Cells were lysed in 20 mM Tris-HCl 7.4 pH at 4 °C for 1 hr ...
-
bioRxiv - Biochemistry 2022Quote: ... and buffer was exchanged four times with 5 mL of 10 mM potassium phosphate buffer pH 7.4 using a Vivaspin 6 Centrifugal Concentrator (Sartorius) at 2,000 rcf and 4°C for 20 min ...
-
bioRxiv - Biochemistry 2020Quote: ... we concentrated the proteins from the supernate by membrane centrifugation using 5 K molecular weight cutoff Vivaspin units of 6 mL (Sartorius Stedim, Goettingen, Germany). The retentate (~300 μL ...
-
bioRxiv - Biophysics 2023Quote: ... or PPIase) were buffer-exchanged into an NMR buffer using either 5 or 3 kDa MWCO centrifugal concentrators (Vivaspin, Sartorius). The NMR buffer contained 10 mM d4-imidazole at pH 6.6 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 3–5 x103 cells were seeded in 96-well plates and viable cell numbers were determined using the Incucyte (Sartorius) Adherent-Cell-by-Cell module ...
-
bioRxiv - Genetics 2023Quote: ... chip activation was made with a freshly prepared mixture of N-hydroxysuccinimide (50 mM in water) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (195 mM in water) for 7.5 min in DPBS (Sartorius, SKU 02-023-5A) (flow rate of 10 μL/min) ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein containing fractions were pooled and concentrated to 5 mg/ml in a Vivaspin 500 (3 kDa) centrifugal concentrator (Sartorius AG), after which they were aliquoted ...
-
bioRxiv - Genetics 2023Quote: ... The eluate was washed 3 times with PBS containing 5% Sorbitol and concentrated with Vivaspin 20 100K concentrator (Sartorius Stedim, Bohemia, NY). Vector titer was determined by qPCR with primers and labeled probe targeting the ITR sequence (Aurnhammer et al. ...
-
bioRxiv - Cancer Biology 2021Quote: ... We imaged cells every 6 hours for 4-6 days of treatment using Incucyte Zoom (Sartorius, Michigan, USA). Fluorescence was quantified using four locations in each treated sample by the IncuCyte Zoom 2016B software.
-
bioRxiv - Immunology 2020Quote: ... concentrated to 1-5 mg/ml by ultrafiltration using appropriate VivaSpin devices (Sartorius), were snap-frozen in liquid nitrogen in small aliquots and stored at -80°C.
-
bioRxiv - Microbiology 2023Quote: ... After concentration (Vivaspin 6 Concentrator, Sartorius Stedim Biotech) and desalting (PD 10 Desalting Columns ...
-
bioRxiv - Bioengineering 2019Quote: ... 3 kDa MWCO concentrator (Sartorius), and the protein concentration was estimated using a BCA assay.
-
bioRxiv - Bioengineering 2019Quote: ... 3 kDa MWCO concentrator (Sartorius). Protein concentrations were estimated by BCA assay.
-
bioRxiv - Biochemistry 2021Quote: ... concentrated to 1 mL using a Vivaspin 4 Turbo concentrator (Cut-off 5 kDa; Sartorius), and purified via size-exclusion chromatography with a Superdex S75-10/300 column (GE Healthcare).
-
bioRxiv - Molecular Biology 2022Quote: ... concentrated with a Vivaspin 6 (10 kDa MWCO) (Sartorius), flash-frozen by liquid nitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... concentrated to 5 mg mL-1 using a 100 kDa molecular weight cut off concentrator (Sartorius), and processed immediately for cryo-electron microscopy.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... in the presence of a green mortality probe (1/3000, Incucyte® Caspase-3/7 Green Dye - Sartorius, Germany). Green fluorescence was immediately followed every 90 or 120 minutes with an Incucyte® S3 Live-Cell Analysis system (Sartorius ...
-
bioRxiv - Neuroscience 2023Quote: ... The eluent was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluent was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius). Imidazole was removed from the concentrated eluent using PD MiniTrap Sample Preparation Columns (Cytiva ...
-
bioRxiv - Synthetic Biology 2022Quote: ... passivated (with 20 μL of 50 mM ABC in 1% sodium deoxycholate or SDC) Vivacon 500 3 kDa molecular weight cutoff (MWCO) filtration units from Sartorius Stedim Biotech ...
-
bioRxiv - Molecular Biology 2022Quote: ... concentrated to 3 ml volume (Vivaspin, Sartorius), and further purified on a HiLoad 16/600 Superdex 200 pg (Cytiva ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Caspase 3/7 indicator dye (Sartorius, Germany) and propidium iodide (BD Biosciences ...
-
bioRxiv - Immunology 2023Quote: ... and Incucyte Caspase-3/7 Dye (Sartorius).
-
bioRxiv - Microbiology 2020Quote: ... using the Vivaspin 6 centrifugal concentrator (MWCO 1,000,000 kDa) (Sartorius, Australia), was required for high-quality electron microscopy visualization ...
-
bioRxiv - Microbiology 2020Quote: ... and then concentrated using Vivaspin® 6 polyethersulfone 10 kDa (Sartorius). The purified and concentrated protein was delivered to GeneScript-Nanjing to raise the anti-rabbit polyclonal antibody.
-
bioRxiv - Microbiology 2020Quote: ... and then concentrated using Vivaspin® 6 polyethersulfone 10 kDa (Sartorius). Concentrations of the purified proteins were measured using BCA protein assay kit (Beyotime ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The eluted complex was concentrated using Vivaspin 6 Centrifugal Concentrators (Sartorius), and imidazole was removed from the protein solution by applying the sample to a PD MiniTrap Sample Column (Cytiva ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were plated at 20,000 cells/ml in Geltrex-coated 96-well plates and treated with different doses of inhibitor in the presence of 1:1000 IncuCyte® Caspase-3/7 Dye for Apoptosis (Sartorius). Doubling times were calculated by determining cell counts over multiple days with the IncuCyte Live Cell Analysis System cell-by-cell module (Sartorius).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were incubated for 3 hours with a red viability probe (1/3000, Incucyte® Nuclight Rapid Red Dye - Sartorius, Germany) incorporated by the nuclei of living cells ...
-
bioRxiv - Cell Biology 2021Quote: ... concentrated to 300-350 µl using Vivaspin 6 30,000 MWCO concentrators (Sartorius), and loaded onto a Superdex 200 10/300 GL column equilibrated in SEC Buffer (25 mM Tris pH 8.0 ...
-
bioRxiv - Plant Biology 2022Quote: ... then concentrated using Vivaspin® 6 concentrators (Sartorius Stedim Lab Ltd, UK) and stored at -80°C before use.
-
bioRxiv - Plant Biology 2022Quote: ... then concentrated using Vivaspin® 6 concentrators (Sartorius Stedim Lab Ltd, UK) and stored at -80 °C before use.