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Citations for BioLegend :
1 - 50 of 5990 citations for SpectraDye Antibody Labeling Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Flow cytometry labeling (without inclusion of Feature Barcoding antibodies from BioLegend) was performed in PBS supplemented with 2% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were used for surface receptor labeling: CD45 (1:200, BioLegend, 103112), CD11b (1:200 ...
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bioRxiv - Immunology 2020Quote: ... αβTc were depleted by labeling with anti-TCRαβ PE antibodies (BioLegend, San Diego, CA, USA) followed by anti-PE microbeads (Miltenyi Biotec) ...
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bioRxiv - Biophysics 2019Quote: ... For labeling of CD19 we used the Alexa Fluor 594 anti mouse CD19 antibody (BioLegend).
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bioRxiv - Neuroscience 2023Quote: ... and HashTag antibodies (1 µl for labeling ∼1 × 106 nuclei, BioLegend, TotalSeqB anti-Nuclear Pore) in order to enrich neurons and multiplex samples ...
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bioRxiv - Genomics 2022Quote: ... was used according to manufacturer’s recommendations: intracellular labeling with APC anti-GATA3 antibody (2.5 ul antibody per million cells in 100 ul) (Biolegend #653806) and PE anti-DYKDDDDK Tag antibody (0.6 ul antibody per million cells in 100 ul ...
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bioRxiv - Biophysics 2023Quote: ... Antibodies used for cell labeling were as follows: APC anti-human CD18 (clone LFA-1/2, Biolegend), and Propidium Iodide solution (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies were used for labeling cells: PE rat anti-mouse CD45 (Clone 30-F11; BioLegend), FITC rat anti-mouse CD3 (Clone 17A2 ...
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bioRxiv - Biochemistry 2020Quote: ... Traditional antibodies based labeling procedure was done by using c-Myc Antibody (9E10, Cat # 626801, BioLegend, USA; incubation 1 h at 4°C) and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143 ...
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bioRxiv - Cell Biology 2023Quote: ... primary conjugated antibodies (AF647) were used directly for cell labeling and included mouse IgG1,k isotype (BioLegend, 400130), mouse IgG2b isotype (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Each B cell sample was split into two aliquots of at least 500,000 cells for labeling with different TotalSeqC anti-human cell hashing antibodies (BioLegend). Cells at a concentration of 5 million/ml were first incubated with human Fc block (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation on ice with labeling cocktail consisting of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer ...
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bioRxiv - Immunology 2020Quote: ... After labeling with PE conjugated anti-TNF (BioLegend, 506306) and PE/Cy7 conjugated anti-IFNγ (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Absolute calibration of pMHC surface density was done as previously described by labeling with an excess of Alexa Fluor 488 labeled anti-HLA antibody (Biolegend, San Diego) and fluorescence determination.
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bioRxiv - Neuroscience 2021Quote: ... Immunogold labeling was performed for GluA2 (BioLegend, San Diego, CA), GluA3 (Synaptic Systems) ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by multiplexing with hashtag labeling of TotalSeq-A0303 (BioLegend, 155805), TotalSeq-A0304 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... For panels requiring streptavidin labeling cells were stained with streptavidin-BV570 (Biolegend). Intracellular staining for Cre (Cre-AF488 Novus Biologicals ...
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bioRxiv - Immunology 2020Quote: ... anti-Neu5Gc antibody kit were from Biolegend, London ...
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bioRxiv - Cell Biology 2024Quote: ... PE anti-human CD117 antibody (KIT; Biolegend, 313204), or Biotin anti-human CD117 (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... PE anti-human CD117 antibody (KIT; Biolegend, 313204) were used ...
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bioRxiv - Immunology 2023Quote: ... and the fluorescence-conjugated antibodies: c-Kit (#105812, BioLegend), Sca-1 (#108126 ...
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bioRxiv - Cancer Biology 2021Quote: ... We excluded dead cells in samples originating from transplanted 3T3 tumors using Zombie Aqua labeling (423102, Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend) and Sca-1 (Pacific Blue ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend) and Sca-1 (Pacific Blue ...
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bioRxiv - Immunology 2024Quote: ... ELISA kits and all fluorescently tagged antibodies were purchased from BioLegend. Pam3 ...
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bioRxiv - Immunology 2022Quote: ... Proliferation was determined by labeling T cells with the Tag-IT Violet Proliferation Cell Tracking Dye (Biolegend, #425101), according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend, 1/100), Sca-1 (Pacific Blue ...
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bioRxiv - Neuroscience 2023Quote: ... double immunofluorescence labeling with detergent was performed with the anti-Aβ mAb 4G8 conjugated with Alexa Fluor 647 (BioLegend) and R3VQ conjugated with 5-TAMRA (see Sup ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend, dilution 1/100), Sca-1 (APC-Cy7 ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in labeling buffer (1% BSA in PBS) and underwent Fc blocking for 10 minutes on ice using Human TruStain FcX (BioLegend). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were first processed for surface marker labeling by incubation with anti-CD8-BV421 and anti-CD4-BV650 (Biolegend) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: Mitochondrial mass was determined in DCs by fixing the cells with PFA 4% and labeling them with the probe MitoSpy Green FM (Biolegend). Green fluorescence was analyzed by flow cytometry (FACScan ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained with fluorescent antibodies: APC-conjugated anti-mouse CD117 (c-Kit) (Biolegend) and PE-Cy7-conjugated anti-mouse Ly-6A/E (Sca-1 ...
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bioRxiv - Immunology 2021Quote: The following antibodies and kits were purchased from Biolegend (San Diego, CA, USA): APC/Cy7 anti-CD4 (clone GK1.5) ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... PE/Cy7-conjugated anti-mouse CD117 (c-Kit) antibody (1:200, Biolegend, 105814) and FITC-conjugated anti-mouse CD9 antibody (1:500 ...
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bioRxiv - Immunology 2024Quote: ... were isolated ex vivo from mouse peritoneal cavity by intraperitoneal lavage followed by magnetic bead-based enrichment for c-Kit+ cells using anti-CD117 (c-Kit)-APC antibody (Biolegend) and APC microbeads (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantifications of liver tissue EVs were performed using the NTA for particle numbers and apoEVs were detected by Annexin V-APC labeling (640920, BioLegend, USA).
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bioRxiv - Cancer Biology 2022Quote: ... and Zombie Dye Violet Blue (Biolgend; 423114) for 15 minutes at 4degC followed by surface labeling with PE anti-human CD45 (Biolegend; 304008) for 30 minutes at 4degC ...
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bioRxiv - Immunology 2020Quote: ... Fluorescence labeled antibodies (CD209,CD206 et al) and multi cytokines kit were from Biolegend (San Diego, CA, USA). Specific inhibitors were acquired from MedChemExpress (Monmouth Junction ...
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bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202 ...
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bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
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bioRxiv - Neuroscience 2022Quote: ... Antibodies (BioLegend) used were as follows:
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bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
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bioRxiv - Cancer Biology 2020Quote: ... All other antibodies used for immune fluorescence and flow cytometric analysis and human IL-1beta ELISA kit were acquired from Biolegend. Human was obtained from In vitro experiments were conducted using complete culture media consisting of RPMI 1640 medium supplemented with 20 mM HEPES ...
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bioRxiv - Physiology 2022Quote: ... Samples were incubated with anti-c-kit rat monoclonal antibody (1:500 v/v, ACK2, BioLegend, San Diego, CA, USA) overnight (4ºC) ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
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bioRxiv - Cancer Biology 2023Quote: ... were performed as we described previously48 and according to the Mission Bio protocol using the custom-designed 37-gene myeloid panel kit and 48 oligo-conjugated antibodies against all major BM cell types (Biolegend). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Comprehensive surface immunophenotyping was performed using LEGENDScreen Human PE Kit containing 371 pre-titrated antibodies and 354 unique specificities (BioLegend). The experimental run was multiplexed using GFP+ NCI-H358 cells resistant to sotorasib combined with non-fluorescent ...