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Citations for BioLegend :
1 - 50 of 3749 citations for Siglec 5 Human HEK293 His Flag Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... recombinant Siglec 5-Fc chimeric protein (Biolegend, San Diego, CA) was added and incubated for 2hrs at RT ...
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bioRxiv - Immunology 2022Quote: ... Cells were than stained with Siglec-5 Fc chimeric protein (Biolegend, San Diego, CA) pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... added with 5 µl of Human TruStain FcX™ Fc Blocking reagent (Biolegend). Cell suspensions were then incubated at 4°C for 10 mins ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with 5 µL Fc-block (Human TruStain FcX, Biolegend 422302) and 1.5 µL of S167-180 HLA class II PE-conjugated tetramer for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 50 µL FACS containing 5 µL human Fc-block (Biolegend, 422302), and blocked for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: ... Siglec F (S17007L, BioLegend), CSF1R (AFS98 ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
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bioRxiv - Developmental Biology 2023Quote: ... Following 5 µl of the Human TruStain FcX (Fc Receptor Blocking Solution, BioLegend, #422302) were added to the sample and incubated for 10 min on ice ...
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bioRxiv - Microbiology 2022Quote: ... Siglec-F (PE, S17007C, BioLegend), TCRβ (PE/Cy7 ...
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bioRxiv - Immunology 2023Quote: ... Siglec F-APC (BioLegend, S17007L), CD11b-PeCy7 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend).
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend). Phenotyping was performed on a NovoCyte Advanteon flow cytometer and FACS was performed on a BD LSRII.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL Binding Buffer containing 100 nM M1 anti-FLAG antibody labeled with Alexa Fluor 488 and Alexa Fluor 647 anti-human IgG Fc (BioLegend) diluted 1:100 (v/v) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL Buffer B containing 100 nM M1 anti-FLAG antibody labeled with Alexa Fluor 488 (In house) and Alexa Fluor 647 anti-human IgG Fc (BioLegend) diluted 1:100 (v/v) ...
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bioRxiv - Immunology 2019Quote: ... Siglec F-PE (E50-2440, Biolegend) CD103 Biotinylate (M290 ...
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bioRxiv - Immunology 2022Quote: ... anti-CD169 (Siglec-1; 3D6.112; BioLegend), anti-CD205 (205yekta ...
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bioRxiv - Immunology 2023Quote: ... PE-labeled Siglec F (S17007L, BioLegend), Pacific Blue-labeled B220 (RA3-6B2 ...
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bioRxiv - Immunology 2024Quote: ... Siglec-H (BioLegend, #129603, clone 551), CD319 (BioLegend #152003 ...
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bioRxiv - Immunology 2023Quote: ... with a difference that the cells were Fc blocked with both human Fc blocker (Human TruStain FcX, Biolegend) and mouse Fc blocker (TruStain FcX™ PLUS (anti-mouse CD16/32 ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated with human Fc receptor block (BioLegend), and stained with cell surface antibodies (1:100 anti-CD137-APC [clone 4B4-1 ...
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bioRxiv - Immunology 2022Quote: ... APC-conjugated anti-human IgG (Fc) (Biolegend), APC-Cy7-conjugated anti-CD3 antibody (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... and human FC block (1:50, Biolegend), and incubated for 20 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2023Quote: ... Human B7-H2-Fc Chimera (Biolegend, 599904), Human SNAP43 (SNAPC1 ...
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bioRxiv - Immunology 2023Quote: ... The anti-human IgG Fc Antibody (BioLegend) and the Sheep Anti-Rat IgG Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2022Quote: ... Human TruStain FcX FC blocking reagent (BioLegend) was added according to manufacturers’ instructions for 10min on ice ...
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bioRxiv - Microbiology 2021Quote: ... Mouse IgG and human IgG were detected with separate secondary antibodies (goat anti-human IgG Fc, goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking buffer and PNPP as described above.
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bioRxiv - Cancer Biology 2019Quote: ... and Siglec F (PE) (Biolegend, 1:200). Fluorescence Minus One (FMO ...
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bioRxiv - Immunology 2022Quote: ... Siglec F-PE (Biolegend, Cat. No. 155505), and Ly6C-AF647 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... TotalSeq-A-labeled Siglec F (S17007L, BioLegend), TotalSeq-A-labeled CSF1R (AFS98 ...
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bioRxiv - Immunology 2021Quote: ... Human Fc receptors (FcRs) were blocked using Human TruStain FcX™ (BioLegend). In samples stained with multiple BD Horizon Brilliant reagents ...
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bioRxiv - Cancer Biology 2023Quote: Human PBMCs were treated with Fc blocking solution (Human TruStain FcX, Biolegend) and stained with the following fluorescent antibodies from Biolegend (unless otherwise stated) ...
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bioRxiv - Immunology 2020Quote: ... Mouse anti-human IgG Fc APC (BioLegend, 409306) was used for secondary antibody staining ...
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bioRxiv - Cancer Biology 2022Quote: ... or anti-human Fc block (#422302, BioLegend, USA). Surface markers were stained with appropriate antibodies for 20 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Human TruStain FcX (Fc receptor blocking solution, BioLegend) was used to avoid nonspecific binding ...
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bioRxiv - Molecular Biology 2023Quote: ... for 15 mins at room temperature and Fc blocked (2.5% human Fc block, BioLegend) for a further 10 mins at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Fc block was performed with a combination of Fc block -Human Tru Stain Fc block (Catalogue No. 422301, Biolegend), Nova Block Solution ...
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bioRxiv - Cancer Biology 2023Quote: IGSF3-Fc or normal human IgG (250 ng) and APC-conjugated anti-human IgG-Fc antibody (BioLegend, San Diego, CA, USA) was added to 1 × 105 of MS1 cells suspended in 100 µL of FACS buffer (0.5% BSA/HBSS ...
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bioRxiv - Immunology 2019Quote: ... an Fc block step was performed by resuspending cells in 50μl of Fc block prepared in PBS/5%BSA (room temperature) using 5μl of Human Trustain FcX (Biolegend) per sample i.e ...
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bioRxiv - Immunology 2021Quote: ... Human Fc receptors were blocked using human TruStain FcXTM (BioLegend Cat# 422302, RRID:AB_2818986).
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bioRxiv - Immunology 2022Quote: ... Cells were blocked with human Fc receptor blocking solution (Human TruStain FcX, Biolegend) for 10 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... A549 were blocked with human Fc receptor blocking solution (Human TruStain FcX, Biolegend) for 10 minutes on ice ...
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bioRxiv - Microbiology 2022Quote: ... PE anti-Siglec F (Clone E50-2440, Biolegend), PE-Cy7 anti-CD64 (Clone X54-5/7.1 ...
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bioRxiv - Microbiology 2022Quote: ... Siglec-F (PE Dazzle 594; clone S17007L; Biolegend), Ly6G (Spark Blue 550) ...
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bioRxiv - Genomics 2023Quote: ... CD169/Siglec-1 (Clone 7-239, Biolegend 346002), or CD209/DC-SIGN (Clone 120507 ...
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bioRxiv - Immunology 2023Quote: ... purified anti-mouse Siglec-E antibody (M1305A02, BioLegend) was added at a final concentration of 10 µg/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (Extended Data Table 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with human Fc receptor block (BioLegend) for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (1:20 anti-CD3-Am Cyan [clone SK7 ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked using human TruStain FcX (BioLegend), and cells were then subsequently stained for surface markers anti-CD11c-FITC (Bu15 ...