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Citations for BioLegend :
1 - 50 of 1943 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Purified mouse anti-SARS-CoV-2 spike protein S2 antibody (1:3000 dilution, BioLegend) and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human SARS-CoV-2 spike S1 IgG lyophilized antibody (Clone AM009105, BioLegend) was used as a standard.
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bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
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bioRxiv - Immunology 2023Quote: ... biotinylated SARS-CoV-2 Spike RBD protein was mixed with Streptavidin-BV421 (405225, BioLegend) at a 4:1 molar ratio for 1 hour at 4 °C to obtain the antigen probe ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Immunology 2023Quote: ... samples from animals that were vaccinated with the recombinant GPCysR4-I53-50A.30 To exclude I53-50A-binding B cells we included a SARS-CoV-1 Spike probe containing I53-50A-biotin which was labeled with streptavidin AF647 and streptavidin BV421 (both Biolegend). After a 2 day culture with interleukin (IL)-21 and irradiated mouse fibroblast (L cells ...
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bioRxiv - Immunology 2022Quote: ... as well as SARS-CoV-2-S1 (biotin-coupled; Acro; #S1N-C82E8) and fluorochrome-coupled streptavidin (Biolegend). The anti-ST6GAL1 and anti-FUT8 Abs were labeled with Alexa Fluor 488 labeling kit (Life Technologies GmbH ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated SARS-CoV-2-S1 Protein (Biolegend 793804) Anti-Biotin (409003) ...
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bioRxiv - Immunology 2021Quote: ... The panel for the cohort that received the SARS-CoV-2 spike prime followed by the RBD homotrimer cocktail boost also included SARS-CoV RBD-APC (streptavidin-APC from BioLegend) and WIV1-CoV RBD-BV650 (streptavidin-BV650 from BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) was purchased from BioLegend.
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bioRxiv - Immunology 2024Quote: ... and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution) or a purified rat anti-SARS-CoV-2 spike protein S1 antibody (1:1000 dilution, BioLegend) and HRP-conjugated goat anti-rat secondary antibody (1:3000 dilution ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated to APC-streptavidin (BioLegend, 405207). After washed twice ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 ΔRBM RBD-APC (streptavidin-APC from BioLegend). Conjugations were performed as previously described (65) ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 ΔRBM RBD-PE/Cy5.5 (streptavidin-PE/Cy5.5 from BioLegend). The panel for the cohort that received the SARS-CoV-2 spike prime followed by the RBD homotrimer cocktail boost also included SARS-CoV RBD-APC (streptavidin-APC from BioLegend ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... and a SARS-CoV-2 nucleocapsid antibody (clone A20087H, mouse IgG2b isotype, BioLegend) followed by a secondary antibody stain with PE-Cy7-conjugated anti-mouse IgG2b (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated to PE-streptavidin (BioLegend, 405204), SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2022Quote: A 3-plex SARS-CoV-2 Serological IgG Panel (BioLegend LEGENDplex, Cat. No. #741132) was used for measuring IgG titre of the 3 key COVID-19 coronavirus derived proteins ...
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bioRxiv - Immunology 2022Quote: ... or SARS-CoV-2 nucleocapsid peptide pool together with 5μg/ml Brefeldin A (Biolegend) for 16 hours ...
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bioRxiv - Immunology 2022Quote: A 5-plex SARS-CoV-2 Variants Neutralizing Antibody Panel (BioLegend LEGENDplex, Cat. No. #741174) was used to measure the neutralization antibody concentration ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2021Quote: ... the following probes were generated: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences) ...
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bioRxiv - Immunology 2021Quote: ... the following probes were generated: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences) ...
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bioRxiv - Immunology 2023Quote: Antigen-specific B cell subsets were detected using biotinylated SARS-CoV-2 RBD protein (793906; Biolegend) in combination with streptavidin (SAv)-fluorophore conjugates as described [7] ...
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bioRxiv - Immunology 2022Quote: Whole blood inoculated with SARS-CoV-2 was blocked with 5μl of TruStain (Biolegend, San Diego, California, # 422302) for 10 minutes at room temperature and then stained for granulocyte and monocyte markers ...
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bioRxiv - Immunology 2022Quote: Serum samples were screened for neutralization using LEGENDplex SARS-CoV-2 neutralizing antibody assay (BioLegend, San Diego, CA, USA) following manufacturer recommendations ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with anti-CoV-2 Spike RBD mAb (SARS2-02) (13) followed by APC-conjugated goat anti-mouse IgG antibody (Cat# 405308, BioLegend). For surface detection ...
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bioRxiv - Immunology 2021Quote: ... The coronavirus protein panel consisted of the following flurorescent probes: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), WIV1 RBD-BV650 (streptavidin-BV650 from BioLegend) ...
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bioRxiv - Neuroscience 2023Quote: Endothelial cells isolated from brain from SARS-CoV-2 infected mice were stained with fixable viability dye (Zombie Green, Biolegend 423111) and blocked with Fc receptor blockade (anti-mouse CD16/32 ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in 200 uL PBS containing 2% FBS and Fc block and incubated with biotin anti-mouse F4/80 (Clone BM8, BioLegend), CD3 (Clone 500A2 ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Immunology 2020Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated with Fc block (2 µg, Biolegend). LIVE/DEAD™ fixable violet (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... or restimulated on day 11 in 96-well plates with 2.5ug/ml recombinant SARS-CoV-S protein S1 (Biolegend 792906, carrier-free) for 8h in the presence of 1ug/ml Golgiplug and 0.7ug/ml Golgistop ...
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bioRxiv - Immunology 2021Quote: ... and biotin anti-mouse IL-2 (clone JES6-5H4, BioLegend) antibody was used as the secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... or biotin anti-mouse IL-2 (Clone: JES6-5H4, BioLegend) SA-HRP (Jackson immunoresearch ...
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bioRxiv - Immunology 2023Quote: ... and biotin anti-mouse IL-2 (clone JES6-5H4, BioLegend) antibody was used as the secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... stained for 20 min at 4 °C in PBS/2% FCS in presence of Fc receptor blocking reagent (Biolegend) with anti-murine CD326-BV605 ...
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bioRxiv - Immunology 2022Quote: ... and the DAXL-88 (anti-AXL) binding assay used Alexa Fluor 647 AffiniPure anti-Fc tag Goat Anti-Human IgG (410711, BioLegend). Anti-Human CD4 antibody (BD ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: Cells were incubated with anti-CD16/32 (Fc block) prior to staining with the following fluorophore or biotin conjugated monoclonal antibodies purchased from Biolegend, eBioscience ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with a 1:100 dilution of biotin anti-human IgG Fc antibody (clone HP6017, BioLegend) for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained in FC buffer (2% FCS in PBS) with anti-CD3-APC conjugated antibodies (1:80; BioLegend) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000 ...
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bioRxiv - Immunology 2019Quote: The percentage of CAR and ΔLNGFR positive T cells was assessed by biotin-conjugated rhCD19-Fc (Cat # CD9-H5259, ACRO Biosystems, USA) with AF647-conjugated streptavidin (Cat # 405237, Biolegend, USA), and fluorescein isothiocyanate (FITC)-conjugated LNGFR antibody (Cat# 130-112-605 ...
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bioRxiv - Immunology 2024Quote: ... 1-2×106 cells were incubated with Fc receptor blocking reagent (BioLegend) for 5 min at RT ...
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bioRxiv - Immunology 2024Quote: ... washed twice HBSS containing 2% FCS and incubated with Human Trustain Fcx (Biolegend) for 20 min on ice ...