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Citations for BioLegend :
1 - 50 of 1648 citations for Recombinant Rat Il6st Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: His-tagged recombinant soluble CD40L (sCD40L, BioLegend) was incubated with NTA-SUV or plain SUV ...
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bioRxiv - Bioengineering 2021Quote: ... The His tagged ACE2 variants were detected using anti-His mAb (Cat. No. 652505, BioLegend).
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bioRxiv - Neuroscience 2020Quote: ... and IFNγ (recombinant rat IFNγ, Biolegend) at 50 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... CAR expression analysis was performed using incubation with His-tagged recombinant CD123 protein (SinoBiological, Beijing, China) and secondary staining with αHis-PE or αHis-APC (BioLegend, San Diego, CA). Dead cells were excluded from analysis using LIVE/DEAD Fixable Viability Stains 780 or 575V (BD Horizon ...
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bioRxiv - Immunology 2022Quote: ... recombinant Siglec 5-Fc chimeric protein (Biolegend, San Diego, CA) was added and incubated for 2hrs at RT ...
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bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged S1-RBD was detected with Alexa Fluor 488 anti-his tag antibody (clone J095G46, Biolegend, UK) 1:5000 in 25 μL PBST + 0.1% BSA per well ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant mouse ICAM-1 and VCAM-1-Fc were from BioLegend. Recombinant mouse MAdCAM-1 was from R&D Systems.
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bioRxiv - Cell Biology 2023Quote: ... 720-IC-200) or 1 μg/ml Fc-VCAM-1 (Recombinant Human VCAM-1-Fc Chimera, Biolegend, 553706) was added to the chamber slide ...
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bioRxiv - Cancer Biology 2023Quote: ... CAR-NK cells were loaded with human recombinant PD-L1- Fc (Biolegend) or ErBb2-Fc fusion proteins (R&D Systems ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were coated with recombinant human E-cadherin-Fc chimera (BioLegend #BLG-779904-25ug) to reduce colony formation and thereby limit the potential impact of paracrine and direct cell signaling in regulating gene expression of related cells 50 ...
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bioRxiv - Immunology 2023Quote: ... Soluble His6-tagged M6G at varying concentrations was incubated for 10 min with HRP-conjugated mouse anti-His antibody (BioLegend) diluted 1:2000 in 0.1% BSA/TBST ...
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bioRxiv - Neuroscience 2020Quote: The cerebellar slices were treated either 3 times 24 hours apart with combinations of recombinant TNF (recombinant rat TNF, Biolegend, San Diego, CA, USA), LTα (recombinant human LT-α ...
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bioRxiv - Biochemistry 2020Quote: ... all cell suspensions were Fc blocked with rat anti-mouse CD16/32 (clone 93, BioLegend, USA) and then stained with 10 μg/mL AlexaFluor647 Armenian hamster anti-mouse CD3ε (clone 145-2C11 ...
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bioRxiv - Immunology 2022Quote: ... followed by rat anti-human Fc antibody conjugated to APC (Cat: 410712, BioLegend; 1:100 dilution) for 15 min on ice in the dark for secondary detection ...
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bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... was used as the primary antibody and rat anti-human IgG Fc antibody conjugated to APC (BioLegend) was used as the secondary antibody ...
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bioRxiv - Immunology 2019Quote: ... Fc-Block was performed (10µg/ml purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse), after which cells were stained with Fixable Viability Staining e780 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Nunc ELISA plates were coated with 1 μg/ml Spike S1+S2ECD-His recombinant protein (SinoBiologicals) in ELISA coating buffer (Biolegend) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Bioengineering 2022Quote: ... and resuspended in 100 μL of the FCM buffer with 5μL of the secondary Antibody (PE-anti human IgG Fc Recombinant, Biolegend, Catalogue 366903) and incubated for 30 minutes at 4°C. ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in FACS buffer and incubated with rat anti-human Fc antibody conjugated to allophycocyanin (Cat: 410712, BioLegend) for 15 minutes on ice ...
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bioRxiv - Physiology 2023Quote: Kupffer cells cultured in 96-well plates were supplemented with 30 μg EPO per well (recombinant rat EPO carrier-free, Biolegend). After 1,5 hours ...
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bioRxiv - Microbiology 2020Quote: ... anti-His PE (Biolegend), and human Hashtag 3 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... mouse ICAM-1-Fc and VCAM-1-Fc were from BioLegend. Recombinant mouse MAdCAM-1-Fc was from R&D Systems (Minneapolis ...
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bioRxiv - Immunology 2019Quote: ... Cell suspensions were incubated for 10 minutes at 4°C with 10μg/ml Fc Block (purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with anti-CD45-PE (1:300 ...
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bioRxiv - Microbiology 2021Quote: ... HRP tagged anti-mouse (Biolegend 405306), Anti-rabbit Alexa Fluor 488 (Invitrogen A27206) ...
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bioRxiv - Microbiology 2023Quote: ... HRP tagged anti-rabbit (Biolegend 406401), HRP tagged anti-mouse (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... HRP tagged anti-mouse (Biolegend, 405306), anti-rabbit Alexa Fluor 555 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Fc block was performed with a combination of Fc block -Human Tru Stain Fc block (Catalogue No. 422301, Biolegend), Nova Block Solution ...
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bioRxiv - Immunology 2023Quote: ... Recombinant IFNα (Biolegend), TNFα (PeproTech) ...
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bioRxiv - Immunology 2023Quote: ... and anti-His PE (Biolegend), at 4 °C for 1 h ...
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bioRxiv - Immunology 2019Quote: ... Fc-blocked (FcX, Biolegend) and stained for 30 minutes on ice with a lyophilized core antibody cocktail comprised of markers to allow identification of all major immune subsets (Supplementary Table 1) ...
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bioRxiv - Immunology 2024Quote: ... Fc-blocked (#158002, Biolegend), then stained for surface markers (B220 PE-Cy5 #103210 ...
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bioRxiv - Immunology 2024Quote: ... Fc-blocked (#158002, Biolegend), then stained for surface markers (CD45 APC #157606 and CD4 FITC #100406 ...
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bioRxiv - Immunology 2023Quote: ... recombinant IFNα2 (#592702) and recombinant IFNγ (#570202) were obtained from BioLegend. PNGase F (#P0704S ...
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bioRxiv - Microbiology 2023Quote: ... and the Fc receptor was blocked with an anti-Fc receptor antibody (BioLegend). The cells were stained with a cocktail of antibodies against three surface makers ...
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bioRxiv - Biochemistry 2020Quote: ... HA-Flag-tagged and GFP-tagged proteins were detected using HRP-conjugated monoclonal anti-HA (1:5000, BioLegend #901501) or polyclonal anti-GFP antibodies (1:2000 ...
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bioRxiv - Bioengineering 2023Quote: ... APC-conjugated His-tag antibody (Biolegend) was added to the cells treated with no nanobody proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-His secondary antibody (Biolegend, #362607) was used to detect IgG4-His primary antibodies ...
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bioRxiv - Immunology 2021Quote: Recombinant mouse GCSF (BioLegend) was injected intraperitoneally (125μg/mouse ...
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bioRxiv - Immunology 2019Quote: ... recombinant murine SCF (BioLegend), recombinant murine G-CSF (BioLegend) ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant mouse IFNβ (BioLegend), PALA (Open Chemical Repository of the Developmental Therapeutics Program NCI/NIH) ...
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bioRxiv - Bioengineering 2024Quote: ... Recombinant human FcγIIa (Biolegend), FcγRIIb (Biolegend) ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were blocked using an Fc blocking antibody (Biolegend Fc-block clone: 2.4G2) and lymphocytes were stained with CD3 and CD4 ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... for 15 mins at room temperature and Fc blocked (2.5% human Fc block, BioLegend) for a further 10 mins at room temperature ...