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Citations for BioLegend :
1 - 50 of 982 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... splenocytes were stained for 30 min at RT with Zombie Green™ Fixable Viability Kit (BioLegend) and were blocked with anti-mouse CD16/32 (BioLegend) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... cells were stained for 30 min at RT with Zombie Green™ Fixable Viability Kit (BioLegend) and were blocked with anti-mouse CD16/32 (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 30 min at RT with Zombie Green™ Fixable Viability Kit (Biolegend) and were blocked with anti-mouse CD16/32 (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: Lung single-cell suspensions were stained for 30 min at RT with Zombie Red™ Fixable Viability Kit (BioLegend) and were blocked with anti-mouse CD16/32 (Biolegend ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... an aliquot of 3×105 cells in 1ml MACS buffer was incubated at RT for 15 minutes with 1:500 dilution of Zombie UV™ Fixable Viability Kit (Biolegend). Cells were stained with 1 µl of anti-GFP antibody (Santa Cruz ...
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bioRxiv - Cancer Biology 2020Quote: ... BrdU-FITC antibody staining for 20 min at RT (BioLegend #364104) and 20 min incubation with PI solution (100 µg/ml ...
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bioRxiv - Immunology 2024Quote: ... and stained for 15 min at RT with annexin V (Biolegend, 640919).
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bioRxiv - Biophysics 2020Quote: ... astrocytes were labelled 1 hour at RT with anti-GFAP (1:150,644702, BioLegend) for staining GFAP ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 1h at RT using IC fixation/permeabilization buffer (Biolegend) and cells were then incubated for 20 min at RT in permeabilization buffer (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... at RT with either human IgG1 isotype control (Biolegend #403502, 0.47 mg/mL), mAb C7 (0.47 mg/mL) ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for at least 60 min at RT (BioLegend, San Diego, CA, USA). Slides were washed 2 times in 0.5% BSA/PBS and once in PBS at RT ...
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bioRxiv - Immunology 2022Quote: ... we lysed red blood cells using RBC Lysis Buffer for 5min at RT (BioLegend). RBC Lysis Buffer was quenched with MACS buffer and washed once ...
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bioRxiv - Immunology 2023Quote: ... and cells were then incubated for 20 min at RT in permeabilization buffer (Biolegend). Intracellular staining was performed for cytokines using antibodies for IL-5 ...
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bioRxiv - Cell Biology 2023Quote: ... and DRAQ5 (1ng/mL, incubation 5 minutes at RT; BioLegend, San Diego, CA, USA). Samples were measured using a FACS Canto II (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked for 1 hour at RT with 1x ELISA dilutant buffer (Biolegend Cat# 421203), then washed twice with 1x wash buffer ...
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bioRxiv - Immunology 2019Quote: ... at RT in the dark using the following antibodies: anti-CD3–PerCP (clone OKT3; BioLegend), anti-CD19– Brilliant Violet 510 (clone HIB19 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were collected and fixed for 20 minutes at RT with fixation buffer (Biolegend 420801), then samples were run on a BD LSRII (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... cells were assessed for viability using a 30 min RT incubation with Zombie NIR (BioLegend) diluted in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and permeabilized for 15 minutes at RT in Intracellular Staining Perm Wash Buffer (Biolegend, 421002), before staining with Recombinant anti-Cytokeratin 8 antibody Alexa Fluor 647 (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained for 15 minutes at RT with 100 μL of Zombie UV dye (BioLegend) diluted 1:500 in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... washed in PBS and incubated for 1h at RT with anti-IL2 JES6-5H4 (APC, Biolegend), anti-IFNγ XMG1.2 (BV421 ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were incubated for 30 min at RT with primary antibodies (Biolegend 844301, anti-Cas9 Clone 7A9) diluted at 1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... ADT and HTO additive primers were added to the RT reaction according to the manufacturer’s instructions (BioLegend). RNA and ADT/HTO libraries were separated using SPRIselect reagent and libraries were prepared separately according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Following a wash, cells were incubated (RT, 10 min) with Rhesus Fc Receptor Binding Polyclonal Antibody (Biolegend). Desired staining antibodies were added directly to this mixture at indicated dilutions (Supplemental Table 27 ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at RT after adding Human TruStain FcX (1:200 dilution, Biolegend, San Diego, CA) for 5 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... and subsequently washed twice prior to incubation in the dark at RT with suitably matched secondary antibodies (Biolegend 406605 ...
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bioRxiv - Cancer Biology 2022Quote: ... for one hour at RT and stained with the following antibodies: Alexa Fluor 488 anti-NESTIN (#656812, Biolegend), Alexa Fluor 555 anti-S100B (#ab274881 ...
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bioRxiv - Neuroscience 2019Quote: ... RT incubation in 2X SSC containing 8 µg/µl Brilliant Violet 421™ Streptavidin (BioLegend, Cat. No. 405225) and 0.05% Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... before washing and transferring to PBS and treating with Zombie NIR (BioLegend, 1:100, 15 min at RT) to label dead cells ...
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bioRxiv - Immunology 2022Quote: ... Cell surface staining was performed at RT for 15 minutes with a panel of antibodies purchased from Biolegend, BD Biosciences or Miltenyi (Table S13) ...
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bioRxiv - Immunology 2023Quote: ... was added to the wells for 1 hour at RT prior to developing with 3,3’,5,5’-tetramethylbenzidine (TMB) substrate solution (BioLegend). The reaction was stopped by adding 0.1M sulfuric acid and the optical density was determined at 450 nm using a SpectraMax iD5 spectrophotometer.
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bioRxiv - Developmental Biology 2023Quote: ... then for 1 hour at room temperature (RT) with biotinylated secondary antibodies and finally with fluorochrome-labeled (BioLegend) or peroxidase-labeled streptavidin (Beckman Coulter) ...
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bioRxiv - Immunology 2024Quote: ... Erythrocytes (RBC) were lysed for 10 min at room temperature (RT) using 1x RBC Lysis/Fixation solution (Biolegend). Samples were washed with phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed for 1 h at RT using the True-Nuclear transcription Factor Buffer Set (BioLegend, 424401). Following the fixation ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated in 5% dried non-fat milk powder dissolved in TBS-Tween 0,1% (TBST) for 1 h at room temperature (RT) and subsequently incubated with primary anti-HA antibody (Biolegend) followed by incubation by secondary goat HRP-conjugated anti-mouse IgG (Promega) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... primary antibody 4G8 was incubated overnight at a 1:1000 dilution at room temperature (RT) (Biolegend, San Diego, CA). HRP-linked secondary goat anti-mouse antibody at a 1:1000 dilution (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... membranes were incubated with primary antibodies for 1h at RT with shaking (1:2000 anti-filaggrin clone Poly19058, Biolegend; 1:2000 anti-β-actin ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 h at RT and stained with two staining panels: (1) anti-CD3 (clone UCHT1; #300415, BioLegend, USA) and anti-CD14 (clone HCD14 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Immunology 2021Quote: ... Cells were labeled with live/dead dye (Zombie Aqua) for 10 min at RT and blocked with human TruStain FcX (BioLegend) for 20 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells suspensions of 100 μL were incubated for 15 min at RT with 2 μL Human TruStain FcX (Biolegend, 422301) to prevent nonspecific Fc receptor binding ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then blocked (PBS-0.25% Triton X-100+ 1% BSA (bovine serum albumin)) for 1h at RT and then primary antibodies (anti-βIII tubulin (Tuj1, 1:1000, BioLegend), anti-NFH (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were blocked with 5% bovine serum albumin (BSA) and 5% goat serum for 1hr at RT and incubated with primary antibodies (anti-βIII-tubulin antibody [BioLegend]) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in TBS-T/5% BSA for one hour at RT and incubated overnight at 4 °C with anti-P62 (SQSTM1) (1:500, #814802, BioLegend), anti-CD107b (LAMP-2 ...
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bioRxiv - Immunology 2022Quote: ... cells were stained with LIVE/DEAD Far Red viability for 20 minutes at RT and then with anti-CD32 (FITC, Biolegend) and anti-CD3 (PerCP ...
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bioRxiv - Neuroscience 2019Quote: ... slides were incubated for 1.5 hrs at RT with a cocktail of secondary fluorescence-conjugated antibody Alexa488 and DAPI (4’,6-diaidino-2-phenylindole, dilactate; BioLegend) to visualize nuclei ...
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bioRxiv - Immunology 2020Quote: ... for 30’ at RT in PBS containing 2% FCS and 1 mM EDTA prior to secondary staining with streptavidin-BrilliantViolet 510 (Biolegend) for 15’ on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... in 100ul PBS for 10min at RT followed by surface staining with antibody specific for CD45 (30-F11, PercPcy55, 1/300, Biolegend). Tumor cells (for RNA-seq ...
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bioRxiv - Immunology 2021Quote: ... for 15 min at RT and stained intracellularly with Fix/Perm Buffer B and antibodies for TNF (PE-Cy7, clone MAb11, BioLegend), IFN-γ (BV711 ...