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Citations for BioLegend :
1 - 50 of 6666 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... PSC-derived endothelial cells were sorted by gating for CD31+ (PE anti-human CD31, Biolegend, 1:200) cells.
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Cell Biology 2024Quote: ... collected in PBS + 1% FBS and stained for ICAM-1 expression on the endothelial cell surface using APC anti-human CD54 antibody (353111, Biolegend). Flow cytometry analysis was performed and confirmed that ICAM-1 expression was upregulated following overnight stimulation with TNF in each of the co-culture experiments.
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bioRxiv - Cancer Biology 2020Quote: ... endothelial cells (CD31) and epithelial cells (EpCAM clone G8.8; all from Biolegend).
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Following antibodies were purchased and used for aortic cell staining: CD45 (30F-11, BD Bioscience and Biolegend), CD31 (MEC13.3 ...
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bioRxiv - Immunology 2022Quote: ... lymphatic endothelial cell marker mouse monoclonal antibody (Cat# 916602, Biolegend) and 1:100 dilution of CD31/PECAM-1 ...
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bioRxiv - Immunology 2022Quote: ... Nalm6 and primary B cells were labeled with PE/Cy7 anti-human CD19 Antibody (Biolegend) and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2 ...
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bioRxiv - Genomics 2019Quote: ... Primary antibodies (anti-human CD47-APC; clone CC2C6, BioLegend, Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... CD31+ (endothelial marker [BioLegend: 102410]), and CD45-(white blood cell marker [BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice after the primary stain and incubated with PE–anti-human Fc antibody (Biolegend, 410708) in MACS buffer for 20 min on ice ...
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bioRxiv - Cell Biology 2021Quote: Cells dissociated from primary HMEC strains (passage 4) were stained with anti-human CD271-PerCP/Cy5.5 (Biolegend #345122) and anti-human CD133-PE (Biolegend #372804 ...
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bioRxiv - Molecular Biology 2022Quote: ... the primary antibodies mouse-anti-human SCARB1 (1:100; Biolegend, clone m1B9) and goat-anti-human MERTK (1:40 ...
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bioRxiv - Cell Biology 2020Quote: ... Immunostaining of specific receptors was performed by incubating cells overnight with AlexaFluor 647 conjugated primary antibodies (human CD4: OKT4, dilution: 1:100, source: Biolegend; human CD45 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Isolated aortic single cells were stained with Fc receptor blocker (TruStain FcX anti-mouse CD16/32; 101319; BioLegend, San Diego, CA, USA, 1:100) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... using primary antibodies for mouse anti-human βIII-tubulin (1:500; Biolegend, cat. 801202), rabbit anti-human βIII-tubulin (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibody Mouse IgG1 anti-human CD11a was purchased from Biolegend (San Diego, CA). Secondary antibody was horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (CD45+ immune cells: 1:50 anti-CD45-VioGreen [Miltenyi Biotec, Germany]; CD31+ endothelial cells: 1:2000 anti-CD31-PE [BioLegend, CA]/1:50 anti-CD45-VioGreen [Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary antibodies were used at 1:1000 dilution: mouse anti-human CD63 (clone H5C6, Biolegend); mouse anti-human CD81 (TAPA-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... peripheral blood and spleens were harvested from each mouse and the infiltrated primary cells were identified using flow cytometry by staining for human CD45 antibody (BioLegend; BLG-368522; 1:200).
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bioRxiv - Immunology 2022Quote: ... Cells were blocked with human Fc receptor blocking solution (Human TruStain FcX, Biolegend) for 10 minutes on ice ...
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bioRxiv - Immunology 2021Quote: Cells were incubated with Human FcX (Biolegend) to prevent non-specific antibody binding ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibodies used in this study were Ultra-LEAF™ Purified anti-human CD63 H5C6 (Biolegend), Anti-Cytomegalovirus Glycoprotein B antibody [2F12] (ab6499 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human leukemia cells were labelled with a FITC anti-human CD45 antibody (368508; BioLegend) then sorted using a BD FACS Aria II Cell Sorter.
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bioRxiv - Cancer Biology 2022Quote: Samples were labelled and imaged with the following primary antibodies: anti-human CD41 (BioLegend Cat# 303702, RRID:AB_314372), anti-human CD34 (BioLegend Cat# 343502 ...
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bioRxiv - Cancer Biology 2022Quote: ... was used as the primary antibody and rat anti-human IgG Fc antibody conjugated to APC (BioLegend) was used as the secondary antibody ...
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bioRxiv - Immunology 2022Quote: The untouched resting B cells were isolated from the human tonsils by depletion of other cells using MojoSort Human B Cell (CD43-) Isolation Kit (BioLegend), whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... Human Th1 cell released cytokines were analyzed by LEGENDplexTM Human Th1 Panel (Biolegend, Cat# 741035). Five human T cell cytokines including IL-2 ...
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bioRxiv - Immunology 2024Quote: ... Human cells were blocked by Human TruStain FcX Fc receptor blocking solution (#422301 from BioLegend). Flow cytometry analysis was performed using an AccuriC6 Plus platform ...
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bioRxiv - Immunology 2022Quote: ... and the MojoSort Human CD8 T cell (BioLegend) isolation kit ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with Human TruStain FcX (Biolegend), surface-stained with CD16-eFluor450 ...
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bioRxiv - Microbiology 2023Quote: ... the LEGENDScreen™ Human Cell PE Kit (Biolegend) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... PDX tumors were digested to a single cell suspension in Collagenase digest buffer and sorted for human cells using anti human HLA-ABC (Biolegend, 555555), DNA and RNA were extracted using an AllPrep Kit (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... human cells were blocked with Human TruStain FcX Fc Receptor Blocking Solution (Biolegend, #422302, 1:100) in FACS buffer and stained with anti-hCD19-PE-Cy7 and anti-hCD138-PE-Cy7 (Biolegend ...
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bioRxiv - Neuroscience 2022Quote: Sections were stained with the primary antibodies: anti-human CD105 (endoglin, ENG) biotin-conjugated (1:100, BioLegend, 323214), anti-human CD31-Alexa Fluor® 594 conjugated (1:400 ...
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bioRxiv - Bioengineering 2023Quote: ... the primary monocytes were isolated using a monocyte isolation kit (MojosortTM Human CD14+ Monocytes Isolation Kit, BioLegend®), per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies that were used in this study included rabbit anti- human CD271 (1:100, HPA004765, Biolegend), mice anti-human CXCL12 (1:50 ...
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bioRxiv - Immunology 2023Quote: ... slides were incubated with primary antibodies to human phospho-6SRP (Ser235/236) (CellSignaling 4858) or CD45 (BioLegend 304002) at 1:50 dilution overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Alexa Fluor 488 or PE–conjugated rat anti-mouse CD31 [platelet endothelial cell adhesion molecule 1 (PECAM1)] antibody (1:200; BioLegend, MEC13.3), Alexa Fluor 647 or FITC–conjugated rat anti-mouse CD326 (Ep-CAM ...
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bioRxiv - Neuroscience 2023Quote: Endothelial cells isolated from brain from SARS-CoV-2 infected mice were stained with fixable viability dye (Zombie Green, Biolegend 423111) and blocked with Fc receptor blockade (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2019Quote: ... human T cells were directly enriched from PBMC by negative selection using MojoSort™ Human CD3 T Cell Isolation Kit (Biolegend, CA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (Extended Data Table 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (1:20 anti-CD3-Am Cyan [clone SK7 ...
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bioRxiv - Immunology 2021Quote: ... the cells were supplemented with Human TruStain FcX (Biolegend) to reduce non-specific antibody staining and 5uM Rhodium-103 (Fluidigm ...
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bioRxiv - Genetics 2020Quote: ... cells were blocked with Human TruStain FcX (Biolegend 422302) and stained at 4°C in a final volume of 50µL with the following antibodies ...
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bioRxiv - Immunology 2022Quote: ... Cells underwent Fc blockade with Human TruStain FcX (BioLegend) and NovaBlock (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... for mouse cells or Human TruStain FcX (422301, Biolegend) for human cells ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with the different cell surface antibodies (1:10 anti-CD112-PE [clone R2.525 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were blocked with human TruStain FcX block (Biolegend) then stained with an extracellular antibody cocktail ...
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bioRxiv - Immunology 2022Quote: Cells were then incubated with Human TruStain FcX (Biolegend) to prevent non-specific antibody binding before staining with Zombie Aqua Fixable Viability Dye (Thermo Fisher ...