Labshake search
Citations for BioLegend :
1 - 50 of 346 citations for Polybrominated Diphenyl Ether Surrogate Spiking Stock 10X Solution 13C12 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... and resuspended in 1x Intracellular Staining Perm Wash Buffer (10x stock solution, diluted to 1x with ddH2O, 420801, BioLegend) at 4ºC for 30 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... and DAPI (1:1000, Biolegend, #422801, stock solution 10mg/ml). Due to the manufacturer discontinuation of the Alexa Fluor® 488 anti-S100 beta antibody ...
-
bioRxiv - Neuroscience 2020Quote: ... Mbp (RRID:AB_2564741, 1:500, Biolegend, SMI-99,), SMI312 (RRID:AB_2135329 ...
-
bioRxiv - Neuroscience 2020Quote: ... Mbp (1:500, RRID:AB_2564741, #SMI-99, Biolegend), TCF7l2 (1:200 ...
-
bioRxiv - Systems Biology 2023Quote: ... before permeabilisation and nuclear staining using PBS supplemented with 2ug/mL DAPI (Biolegend, #422801, stock solution 10mg/ml), 5%FBS (Gibco ...
-
bioRxiv - Genetics 2021Quote: ... mouse anti-MBP (1:4.000; clone SMI 94/SMI 99, BioLegend); rabbit anti-MAG (1:1.000 ...
-
bioRxiv - Bioengineering 2020Quote: ... Staining buffer used was a 1:1000 dilution of stock antibody solution (APC anti-mouse CD45; Alexa Fluor 488 anti-mouse Ly6G, Biolegend) into FACS buffer ...
-
bioRxiv - Immunology 2019Quote: ... cells were permeabilized (Permeabilization wash buffer 10X, BioLegend) and stained with rabbit anti-mouse ATX (Cayman) ...
-
bioRxiv - Neuroscience 2020Quote: ... Antibodies used in western blot are MBP (SMI-99, RRID:AB_2564741, 1:2000, Biolegend), Active β-catenin (#05665 ...
-
bioRxiv - Immunology 2021Quote: ... OKT-3 stock (BioLegend Inc, USA) was diluted to 50mg/ul ...
-
bioRxiv - Microbiology 2021Quote: ... Residual erythrocytes were lysed with 1X RBC lysis buffer (10X, BioLegend), followed by three washing steps with fresh PBS for 10 minutes at 250 × g ...
-
bioRxiv - Cancer Biology 2022Quote: ... Chromium Next GEM Single Cell 3ʹ v3.0 kit (Biolegend, 10X Genomics). Libraries were sequenced on a HiSeq2500 or HiSeq-X (Illumina ...
-
bioRxiv - Cancer Biology 2021Quote: ... Chromium Next GEM Single Cell 3’ Reagent v3.0 Kit (Biolegend, 10X Genomics). Libraries were sequenced on a HiSeq2500 (Illumina ...
-
bioRxiv - Bioengineering 2022Quote: ... CD8 T cells were >99% pure and assessed by flow cytometry staining with CD8-Pacific Blue antibody (Biolegend). Total CD8 T cells were activated using anti-CD3/CD28 coated beads (Invitrogen ...
-
bioRxiv - Immunology 2020Quote: ... 1 µl/ml of CFSE (Biolegend, 5mM in DMSO stock) was added at a final concentration of 5 µM ...
-
bioRxiv - Genomics 2022Quote: ... ADT cocktails (diluted 20 times from original stock) from Biolegend were added onto the tissue and incubated for 30 minutes at 4 °C ...
-
bioRxiv - Immunology 2024Quote: ... PCLS sections were permeabilized with 1X intracellular staining permeabilization wash buffer (from 10X, BioLegend) to capture the nucleocapsid protein using a rabbit anti-SARS-CoV-2 nucleocapsid antibody (Sino Biological ...
-
bioRxiv - Cancer Biology 2020Quote: Samples were stained with 10X Chromium 3’ mRNA capture compatible TotalSeq-A antibodies (Biolegend, USA). Staining was performed as previously described by Stoeckius et ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibodies (anti-GFAP, DAKO, z-0334, 1:1000; SMI-99 [anti-myelin basic protein] Biolegend; 808401 ...
-
bioRxiv - Immunology 2020Quote: ... The purity of the cells was 99 % as analyzed by flow cytometry using the macrophage markers CD11b (M1/70, BioLegend) and F4/80 (BM8 ...
-
bioRxiv - Immunology 2022Quote: ... Overnight fixed cells were permeabilized with 1X Permeabilization buffer (True Nuclear 10X Perm, Catalogue No. 73162, Biolegend) for intracellular staining ...
-
bioRxiv - Bioengineering 2023Quote: ... 10X RBC lysis buffer and antibodies used for flow cytometry were sourced from Biolegend (San Diego, CA). Hematoxylin ...
-
bioRxiv - Cell Biology 2021Quote: ... Development was performed with TMB solution and terminated with Stop solution (Biolegend). Readings were taken with a SpectraMax M2 spectrometer (Molecular Devices ...
-
bioRxiv - Immunology 2020Quote: ... Brefeldin A Solution (1000×) and Monensin Solution (1000×) were purchased from BioLegend. D-Luciferin Potassium Salt (>99% ...
-
bioRxiv - Immunology 2021Quote: ... Brefeldin A Solution (Biolegend) was added at 1X for the final 4 hours ...
-
bioRxiv - Systems Biology 2019Quote: ... FcX blocking solution (BioLegend) was added in a dilution of 1:50.
-
bioRxiv - Microbiology 2020Quote: ... and PI solution (BioLegend) for 15 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... FcX blocking solution (BioLegend) was added at a dilution of 1:50.
-
bioRxiv - Pathology 2022Quote: ... Brefeldin A solution (Biolegend) was added to the stimulated PBMCs to prevent secretion of intracellular cytokines ...
-
bioRxiv - Immunology 2021Quote: ... or PI solution (BioLegend) and were analyzed with Fortessa cytometer (BD Biosciences) ...
-
bioRxiv - Immunology 2020Quote: ... Monensin Solution (420701) (BioLegend); mouse TNF-α (430904 ...
-
bioRxiv - Immunology 2021Quote: Monocyte blocking solution (BioLegend) and Brilliant Stain Buffer (BD ...
-
bioRxiv - Bioengineering 2023Quote: ... Brefeldin A solution (BioLegend) was added at a 1:1000 concentration to inhibit golgi-mediated secretion of proteins and to allow intracellular accumulation of cytokines ...
-
bioRxiv - Immunology 2023Quote: ... Ce3D clearing solution (Biolegend) was added 30 minutes before mounting in a seal frame incubation chamber (ThermoFisher ...
-
bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
-
bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
-
bioRxiv - Immunology 2021Quote: ... and Monensin solution (1x) (BioLegend). A Brefeldin A and Monensin only control was also plated for each infant ...
-
bioRxiv - Immunology 2020Quote: ... Brefeldin A Solution (1000X, BioLegend) was added to each well (dilution 1000-3000-fold depending on the length of the experiment ...
-
bioRxiv - Molecular Biology 2020Quote: ... Propidium Iodide Solution (Biolegend; 421301) was used for the evaluation of cell viability ...
-
bioRxiv - Biophysics 2023Quote: ... and Propidium Iodide solution (Biolegend). Wash cells and then resuspend cells in 350 μL FAC wash buffer (1X DPBS containing 5% FBS ...
-
bioRxiv - Biophysics 2023Quote: ... propidium iodide solution (Biolegend, USA) was added to the dispersed phase at a concentration of 2 µL per 100 µL of cell solution (with 1×106 cells mL-1) ...
-
bioRxiv - Immunology 2021Quote: ... Amplified antibody barcodes were recovered from the supernatant and were processed to generate TotalSeq-C libraries as instructed by the manufacturers (10X Genomics and BioLegend, TotalSeq-C with 10x Feature Barcoding Protocol) ...
-
bioRxiv - Cancer Biology 2020Quote: ... FOXP3 APC (Clone FJK-16s eBioscience) used after cell permeabilization with the kit Perm Buffer (10X) and Fix/Perm Buffer (4X) (BioLegend), according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
-
bioRxiv - Cancer Biology 2022Quote: ... protein expression (ADT) and hashtag (HTO) libraries were generated using Chromium Single Cell 3’ version 2 reagents (10x genomics and Biolegend) per manufacturers’ protocols ...
-
bioRxiv - Immunology 2022Quote: ... Samples sorted for 10x Genomics single-cell RNAseq were individually labelled with an oligonucleotide-linked hashing antibody (Totalseq-C, Biolegend) in addition to the staining cocktail and sorted into a single tube according to the gating strategy shown in Fig ...
-
bioRxiv - Immunology 2023Quote: ... lysis was performed at room temperature for 5 minutes in 1X RBC lysis buffer (10X RBC lysis buffer diluted in mqH2O, 420301, BioLegend) and reaction was stopped by diluting the 1X RBC lysis buffer with 35ml 1X DPBS ...
-
bioRxiv - Cell Biology 2023Quote: ... was then carried out following the Chromium Next GEM Single Cell 3’ protocol for v3.1 reagents from 10X Genomics (CG000206 Rev D) and TotalSeq™-A Antibodies and Cell Hashing protocol from Biolegend (available online at https://www.biolegend.com/en-gb/protocols/totalseq-a-antibodies-and-cell-hashing-with-10x-single-cell-3-reagent-kit-v3-3-1-protocol) ...
-
bioRxiv - Developmental Biology 2024Quote: ... and the resulting cDNA was amplified using as PCR oligos the partial TruSeq read 1 adaptor (provided in the 10x kit) and an additive primer (a partial TruSeq read 2, sequence provided by Biolegend and synthesized by Integrated DNA Technologies) ...
-
bioRxiv - Genomics 2024Quote: ... amplification and sequencing libraries were generated using the Next GEM Single Cell 3’ Kit v3.1 (10X Genomics) with the recommended modifications for compatibility with the TotalSeq-A cell hashing and CITE-seq reagents (BioLegend). Organ donor GEX libraries were sequenced on a NovaSeq 6000 (Illumina ...