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Citations for BioLegend :
1 - 50 of 4356 citations for Peripheral Blood Mononuclear Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (Veri-Cells PBMC, BioLegend) were stained with each novel fluorochrome for 30 minutes and fixed with various fixatives according to the manufacturer’s instructions – Cell Staining Buffer (PBS + 10% Fetal Bovine Serum (FBS)) ...
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bioRxiv - Immunology 2022Quote: ... and peripheral blood mononuclear cells (PBMC) were enriched by density gradient centrifugation over Lymphopure (BioLegend). The residual red blood cells were lysed with ammonium chloride lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... Anonymous healthy donor peripheral blood mononuclear cells (PBMCs; StemCell) were activated with anti-CD3 (OKT3; Biolegend) in the presence of 100 U/mL human IL-2 ...
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bioRxiv - Immunology 2024Quote: ... peripheral blood mononuclear cells (PBMCs) were stained with the following antibodies: allophycocyanin (APC)-anti-CD34 (581; BioLegend), FITC-anti-CD14 (61D3 ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells from blood were isolated from healthy donor peripheral blood mononuclear cells (PBMCs) using the BioLegend human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333 ...
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bioRxiv - Immunology 2021Quote: ... were produced by differentiating peripheral blood mononuclear cells (PBMC) in RPMI 1640 medium containing human M-CSF (10ng/mL, Biolegend) and 10% human platelets-free plasma for 5-7 days (42 ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells from healthy donors were activated in cell culture plates precoated with CD3 (5 μg/mL, BioLegend, San Diego, CA) and CD28 (1 μg/mL ...
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bioRxiv - Immunology 2021Quote: 4*106 peripheral blood mononuclear cells (PBMCs) per patient were first incubated with Human TruStain FcX™ blocking reagent (Biolegend, Cat# 422302) for 10 minutes at 4°C ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Peripheral blood and splenocytes were stained with CD45-PercP (30F-11; BioLegend, 103130), NK1.1-FITC (PK136 ...
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bioRxiv - Cell Biology 2021Quote: ... peripheral blood cells were RBC depleted using ACK as above and stained with an antibody cocktail of CD8-PE (Biolegend, 100708), Mac-1 PE/Cy7 ...
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bioRxiv - Bioengineering 2022Quote: ... Mononuclear cells were blocked with Fc blocking solution (BioLegend) and stained with the following antibody panels ...
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bioRxiv - Cell Biology 2020Quote: ... Engraftment and lineage analysis was determined by staining peripheral blood with CD45.1 (BioLegend Cat#110708; RRID: AB_313497), CD45.2 (BioLegend Cat#109806;RRID ...
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bioRxiv - Cancer Biology 2022Quote: ... was analyzed and monitored longitudinally by weekly bleeding to quantify hCD45+ cells in the peripheral blood by flow cytometry with human CD45-PE and anti-mouse CD45-APC-Cy7 antibodies (Biolegend, San Diego, CA, USA). Mice were monitored closely to detect disease onset and treatment started when hCD45+ cells were detectable in the peripheral blood ...
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bioRxiv - Cell Biology 2019Quote: ... Peripheral blood was surface stained with APC/Cy7 conjugated anti-mouse CD45 antibody (clone 30-F11, Biolegend, San Diego, CA), APC conjugated anti-mouse CD3 antibody (clone 145-2C11 ...
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bioRxiv - Cell Biology 2021Quote: ... the filtered mononuclear cells were additionally incubated with Vcam1-biotin (105704, BioLegend), CD31-FITC (102506 ...
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bioRxiv - Cell Biology 2023Quote: ... The resultant mononuclear cell suspensions were treated with antibodies against VCAM1 (Biolegend) for APC detection of myogenic cells ...
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bioRxiv - Physiology 2019Quote: ... red blood cells were lysed with 1x red blood cell lysis buffer (BioLegend). For organ harvest ...
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bioRxiv - Cell Biology 2022Quote: ... Red blood cells were lysed in red blood cell lysis buffer (BioLegend #420301). The resulting cell suspension was centrifuged for 5 min at 500 x g at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and red blood cells were lysed with 1x red blood cell lysis buffer (BioLegend). For organ harvest ...
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bioRxiv - Cancer Biology 2022Quote: ... mononuclear cells were stained with anti-human CD34 BV650 (Biolegend, Cat# 343624, RRID:AB_2632725) for 20min at RT ...
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bioRxiv - Immunology 2020Quote: ... Red Blood Cell Lysis Buffer (BioLegend) was utilized on tissues before they were passed through 70 μm filters to prepare single cell suspensions ...
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bioRxiv - Cancer Biology 2022Quote: ... After red blood cell lysis (BioLegend), single-cell suspensions were depleted of lineage-committed hematopoietic cells using a Lineage Cell Depletion Kit according to manufacturer’s protocol (EasySep™ ...
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bioRxiv - Immunology 2020Quote: ... Following Red Blood Cell lysis (Biolegend), cells were plated at 3.0E6 cells/ml in 1640 RPMI supplemented with 10% HI FBS ...
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bioRxiv - Immunology 2020Quote: ... red blood cells were lysed (Biolegend), and resulting single cell suspensions were cultured in the above media in flat bottom 48-well Nunclon plates (ThermoFisher) ...
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bioRxiv - Genetics 2021Quote: ... After red blood cell lysis (BioLegend), dead cells were depleted using the dead cell depletion kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Red blood cell lysis (BioLegend #420302) was performed on single cell suspension for 1 min before further downstream processing.
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bioRxiv - Cancer Biology 2023Quote: ... red blood cell lysis buffer (Biolegend) was used ...
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bioRxiv - Immunology 2023Quote: ... A red blood cell lysis (Biolegend) step was performed to remove erythrocytes ...
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bioRxiv - Immunology 2024Quote: ... Following red blood cell lysis (BioLegend), cells were maintained on ice in RPMI 1640 supplemented with 5% FBS ...
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bioRxiv - Immunology 2024Quote: ... and lysing red blood cells (BioLegend). Skin specimens were minced and digested with 3 mg/mL collagenase III (Worthington Biochemical Corporation ...
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bioRxiv - Immunology 2019Quote: Isolated mononuclear cells were washed in PBS and incubated with Zombie Aqua™ dye (BioLegend) to distinguish live and dead cells ...
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bioRxiv - Cell Biology 2022Quote: Mouse blood and bone marrow were treated with red blood cell lysis buffer (Biolegend) prior to counting and staining for flow cytometry (see Table 1).
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bioRxiv - Immunology 2019Quote: ... Following red blood cell lysis (BioLegend, 420301), cells were passed through a 35μm filter ...
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bioRxiv - Microbiology 2021Quote: ... After red blood cell lysis (Biolegend; # 420301), single-cell suspensions were filtered ...
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bioRxiv - Immunology 2021Quote: ... and red blood cells were lysed (Biolegend). Immune cells were then assayed via flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... CD45+ (white blood cell marker [BioLegend: 103114]), PE+ (constitutively expressed RFP) ...
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bioRxiv - Cell Biology 2022Quote: ... a suspension of mononuclear cells was stained with Alexa 700 anti-CD3 (1:100, BioLegend, 100215), PE-Cy7 anti-CD19 (1:100 ...
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bioRxiv - Cell Biology 2019Quote: Apoptosis in mononuclear cell suspensions was assessed using the Annexin V-FITC Apoptosis Kit (BioLegend, 640914) according to manufacturer’s instructions and analyzed via flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... mononuclear cells were treated with TruStain FcX (anti-mouse CD16/32) antibody (Clone 93, BioLegend, USA) and incubated at 4°C for 5 min to block Fc receptors ...
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bioRxiv - Immunology 2021Quote: ... cells were processed with red blood cell lysis buffer (Biolegend) and plated in 1640 RPMI (Biological Industries ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were treated with red blood cell lysis buffer (BioLegend) for 2 minutes and washed with PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Blood was then incubated in red blood cell lysis buffer (Biolegend, San Diego, CA, USA) for 10 min on ice and quenched with 1% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Red blood cells were lysed in blood and spleen samples using RBC-lysis buffer (Biolegend).
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bioRxiv - Immunology 2022Quote: ... Following isolations cells were centrifuged 10 min at 300 g and red blood cells were eliminated using Red blood cells (RBC) Lysis Buffer (BioLegend). This procedure gives >95% of neutrophil population as assessed by flow cytometry of CD15+/CD16+ cells ...
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bioRxiv - Immunology 2019Quote: ... Red blood cell lysis was performed (BioLegend, 420301), followed by centrifugation (400g ...
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bioRxiv - Immunology 2020Quote: ... The preparation underwent Red Blood Cell lysis (Biolegend). cDNA was generated from Quick RNA Microprep (Zymo ...
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bioRxiv - Cancer Biology 2019Quote: ... treated with Red-Blood Cell Lysis Buffer (BioLegend) and passed through a 40μm nylon cell-strainer (EASYstrainer ...
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bioRxiv - Cancer Biology 2023Quote: ... and CD45-(white blood cell marker [BioLegend: 103108). A cutoff on the endothelial cells in the channel of the adhesion molecule antibody (E-selectin [Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from the resulted mononuclear cells using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). Single molecule FISH experiments were carried out using an RNAscope® Multiplex Fluorescent Assay v2 along with an RNAscope® Probe-Mm-Krt14-C2 (ACD cat ...
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bioRxiv - Immunology 2020Quote: Total blood or bone marrow was incubated with 1x red blood cell (RBC) Lysis Buffer (Biolegend) for 10 min on ice ...