Labshake search
Citations for BioLegend :
1 - 50 of 1985 citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... ELISA plates were coated with anti-IL-2 Ab (BioLegend) at a concentration of 0.25ug/ml overnight at 4 °C or 1 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... or plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155-Fc (1 μg/mL; BioLegend) for four days ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Immunology 2022Quote: Splenocytes from TregΔCd226 and TregWT mice were plated in a 96-well plate at a concentration of 1,000,000 cells/mL with plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155 (1 μg/mL; BioLegend) for 15 or 30 minutes at 37ºC ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-2 and TNFα kits (BioLegend) and the experiments were done according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cells were plated in a 24-well plate at a concentration of 500,000 cells/mL on either plate-bound α-CD3ε (2 μg/mL; 145-2C11; BioLegend) and soluble α-CD28 (1 μg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... Legend Max ELISA Kit with Pre-coated Plates (BioLegend) as specified by the manufacturer.
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/ml anti-mouse-IgM or IgG (Biolegend) in NaHCO3 0.1 M pH 9.6 ...
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bioRxiv - Immunology 2021Quote: ... cells were split into 2 new 96-well plates and incubated with freshly prepared supplemented RPMI media with IL-2 (10 ng/ml, BioLegend, #575402). Cells were cultured for a total of 5 days before injection ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/mL of anti-IgE (clone RME-1, BioLegend) and for IgG1 ELISAs ...
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bioRxiv - Immunology 2023Quote: ... The 96-well ELISA plates were pre-coated with purified anti-mouse IL-2 (BioLegend) using coating buffer (0.1 M Na2HPO ...
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bioRxiv - Immunology 2023Quote: ... IL-2 secretion was evaluated with the IL-2 assay ELISA kit (Cat#431801, Biolegend). The absorbance value at 450 nm/570 nm was detected by the microplate reader ...
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bioRxiv - Immunology 2020Quote: ... CD138 (281-2) and c-kit (2B8) from BioLegend.
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bioRxiv - Immunology 2022Quote: ... coated flat bottomed 96 well plates with soluble anti-CD28 (2 μg/mL) (ULTRA-LEAF Biolegend), and with or without human recombinant soluble BAFF at 500 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... the human IL-2 ELISA kit (catalog no. 431801; BioLegend) and human IFN-gamma kit (catalog no ...
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bioRxiv - Cell Biology 2021Quote: ... the concentration of IL-2 in the supernatant was measured using an IL-2 ELISA kit (Biolegend).
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bioRxiv - Immunology 2022Quote: ... IL-2 concentration in harvested supernatant was then analyzed using Human IL-2 ELISA Max Kit (BioLegend) per provided instruction ...
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bioRxiv - Immunology 2024Quote: ... and penicillin in a 96-well plate pre-coated with anti-CD3Ab (2 μg/ml, Biolegend 100360), along with the addition of 5 μg/ml anti-CD28 antibody (Biolegend 102122 ...
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bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...
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bioRxiv - Immunology 2022Quote: ... followed by stimulation with plate bound anti-CD3 (2 μg/ml) and anti-ICOS (5 μg/ml; C398.4A; Biolegend) for 24 hours.
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... ELISA plates (Immulon 2HB 96-well plates, Biolegend) were coated with 100 ng per well (100 μl ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Immunology 2021Quote: ... sort-purified γδ or αβ IELs were seeded at approximately 400K cells per well in round-bottom 96-well plates coated overnight with 2 μg/ml anti-CD3 (145-2C11, BioLegend), 2 μg/ml polyclonal Armenian hamster IgG (BioXCell) ...
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bioRxiv - Genomics 2022Quote: ... flat bottom 96-well plates were coated with 2 μg/mL of anti-CD3 (Clone: 145-2C11, BioLegend, Cat#100302) in PBS overnight at 4°C or 4 hrs at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Lcn-2 levels were determined in fecal supernatants using a LEGEND MAX™ Mouse NGAL (Lipocalin-2) ELISA Kit (BioLegend #443708).
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bioRxiv - Immunology 2022Quote: ... The plate was finally washed eight times and was developed with the TMB substrate kit (Biolegend) according the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Supernatant was collected for IL-2 measurement using an ELISA kit (BioLegend #431801).
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bioRxiv - Immunology 2023Quote: ... followed by testing with a murine IL-2 commercial sandwich ELISA kit (BioLegend).
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bioRxiv - Immunology 2023Quote: ... followed by testing with a murine IL-2 commercial sandwich ELISA kit (BioLegend).
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bioRxiv - Immunology 2023Quote: ... followed by testing with a murine IL-2 commercial sandwich ELISA kit (BioLegend).
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bioRxiv - Immunology 2023Quote: ... followed by testing with a murine IL-2 commercial sandwich ELISA kit (BioLegend).
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bioRxiv - Immunology 2020Quote: ... cells were re-stimulated with plate-bound CD3 and soluble CD28 antibodies with the presence of 20 ng/μl IL-2 (Biolegend #589106) for 3 days before infection with HIV-1 reporter viruses ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were then thawed and plated in 24-well plates (2-2.5 millions/1ml/well) pre-coated with anti-CD3 (2.5 ug/ml, HIT3a clone, Biolegend, San Diego, CA) for 24 hours before harvesting ...
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bioRxiv - Immunology 2022Quote: ... the stained cells were distributed across the three 96-well plates provided with the LEGENDScreen kit (Biolegend), each well containing a unique PE-labeled exploratory antibody as well as isotype controls and blanks ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA) and stained with Zombie Aqua Fixable Viability Kit (BioLegend, 1:200). Living ...
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bioRxiv - Immunology 2024Quote: IL-2 and IFN-γ levels were measured with a sandwich ELISA kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Immunology 2022Quote: ... Sorted naïve 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend, San Diego, CA) and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Immunology 2019Quote: ... 96-well plates (Biolegend) were pre-coated with 100 µl/well of 7×104 PFU of X31 virus in PBS overnight at 4°C ...
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bioRxiv - Pathology 2021Quote: ... Microwell plates (BioLegend, #423501) were coated with purified GM-CSF capturing antibody from ELISA MAX™ Deluxe Set Mouse GM-CSF (Biolegend) ...
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bioRxiv - Bioengineering 2020Quote: Maxisorp plates (BioLegend, 423501) were coated with 1μg/ml anti-Flag antibody (Sigma Aldrich ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 concentrations were analyzed using the respective LEGEND MAX Human ELISA Kits (BioLegend, USA).
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bioRxiv - Immunology 2023Quote: ... or phosphorylated ERK1/2 (pERK1/2; Biolegend), cells were stained with surface markers then fixed with Paraformaldehyde 1% ...
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bioRxiv - Immunology 2021Quote: ELISA plates (Nunc, MaxiSorp ELISA plates, flat bottom, uncoated, BioLegend, San Diego, CA, USA) were coated with recombinant human CTLA-4-Fc (7268-CT ...
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bioRxiv - Cell Biology 2021Quote: ... About 0.3 million cells in 37.5 μl CSM were loaded in each well of a 96-well plate of the Human Cell Surface Marker Screening (phycoerythrin [PE]) Kit (Biolegend). Each well contained 12.5 μl of diluted PE-conjugated antibody in CSM ...
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bioRxiv - Immunology 2021Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2020Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2023Quote: ... Nucleocapsid level on exosomes was measured by ELISA using precoated ELISA plates (Legend Max SARS-CoV2 Nucleocapsid protein ELISA Kit, 448007, BioLegend) according to the manufacturer’s instructions ...