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Citations for BioLegend :
1 - 50 of 123 citations for Fetal + Enhanced fetal bovine serum Aseptically Filled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... They were incubated in high glucose Dulbecco’s’ modified Eagle medium (DMEM) (Capricorn) supplemented with 10% fetal bovine serum (Biolegend), 100 U/mL of penicillin (Lonza ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... kidney epithelial) cells consisted of high glucose Dulbecco’s modified Eagle medium (DMEM) (Capricorn) with 10 % fetal bovine serum (FBS) (Biolegend), 100 U/mL of penicillin (Lonza ...
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1-2 million HL-60 and THP-1 cells were resuspended in PBS containing 1% fetal bovine serum and stained with PE anti-human CD11b antibody (Biolegend). Stained cells were analyzed on FACScalibur flow cytometer (Becton Dickinson) ...
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bioRxiv - Immunology 2021Quote: ... or total combined splenocytes plus bone marrow from WT or c-Rel-/- mice were stimulated for 48 hours in complete RPMI containing 10% fetal bovine serum (FBS) and 1% pen strep with either rmIL-2 (1000 U/ mL; BioLegend), rmIL-15 (100 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of 30 ng/ml GM-CSF (BioLegend) for 9-12 days ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Microbiology 2023Quote: ... opsonized bacteria were re-suspended in sterile PBS with 2% fetal bovine serum and stained with APC-conjugated anti-human C3b (Biolegend 846106) and FITC-conjugated anti-human C5b-C9 (LSBio C210266 ...
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bioRxiv - Microbiology 2021Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of GM-CSF (20 ng/ml, BioLegend, Cat# 576304) for 9-12 days as described in (Dai et al. ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... 2 million splenocytes were resuspended in 50 µl of fluorescence-activated cell sorter (FACS) buffer (PBS with 2% fetal bovine serum) and blocked with TruStain fcX (clone 93; BioLegend, San Diego, CA). The cells were washed and stained to identify B cell subsets with fluorophore-conjugated antibodies against CD45.2 (clone 104) ...
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bioRxiv - Microbiology 2023Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
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bioRxiv - Immunology 2022Quote: ... washed with FACS buffer (PBS supplemented with 1 mM EDTA and 2% fetal calf serum) and stained with a live/dead marker (viability-eF780, eBiosciences) together with CD19-AF700 (HIB19, BioLegend), CD20-PE-CF594 (2H7 ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were washed with FACS buffer (PBS containing 2% fetal calf serum and 1 mM EDTA) and 1 µL of FcX TruStain PLUS (Biolegend) was added to each sample ...
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bioRxiv - Systems Biology 2021Quote: Fetal gonadal cells a were fixed with fixation buffer (420801, Biolegend) at room temperature and in the dark for 20 min ...
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bioRxiv - Biochemistry 2021Quote: ... fetal liver cells were stained with PE-anti mouseTer119 (Biolegend, 116207, 1:200), PE/Cy7-anti CD44 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... 1% bovine serum albumin and 0.3% Triton X-100) then stained with anti-gp100 antibody (clone HMB45, BioLegend) used at 1:30 followed by an anti-IgG2a secondary conjugated to Alexa555 (Invitrogen ...
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bioRxiv - Systems Biology 2019Quote: ... Stained cells were washed with HBH (Hank Balanced Salt Solution (HBSS) with 0.1% bovine serum albumin (BSA) and 10mM HEPES and stained with streptavidin-PerCP/Cy5.5 (BioLegend). Stained cells were washed ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were then washed with HBH (Hank Balanced Salt Solution (HBSS) with 0.1% bovine serum albumin (BSA) and 10mM HEPES) and stained with streptavidin-PerCP/Cy5.5 (BioLegend).
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bioRxiv - Immunology 2020Quote: ... The cells were stained in FACS buffer (PBS containing 0.5% bovine serum albumin and 0.05% sodium azide) with fluorescently labelled antibodies from BioLegend (CD11c-APC/Cy7 ...
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bioRxiv - Immunology 2023Quote: ... sections were blocked with PBS containing 1 % bovine serum albumin (BSA) and 1:300-diluted TruStain FcX™ (Biolegend) for 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently resuspended in staining buffer (PBS with 0.5% bovine serum albumin and 2 mM EDTA) and Fc-blocked using TruStain FcX reagent (BioLegend). Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then blocked (PBS-0.25% Triton X-100+ 1% BSA (bovine serum albumin)) for 1h at RT and then primary antibodies (anti-βIII tubulin (Tuj1, 1:1000, BioLegend), anti-NFH (1:1000 ...
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bioRxiv - Systems Biology 2019Quote: ... blocked in TBST buffer supplemented with 5% bovine serum albumin (BSA) and incubated overnight with primary anti-XBP1s antibody (Biolegend Poly6195) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... or double knockout cells were harvested and washed with PBS containing 1% bovine serum albumin (BSA) and incubated with 5 μL of PE-conjugated EphA2 antibody (Biolegend, SHM16) in 50 μL PBS containing 1% BSA for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: Isolated cells were stained at 4°C in FACS buffer (PBS supplemented with 2.5% bovine serum albumin) with and hematopoietic lineage markers including Ly6G (BioLegend, clone 1A8, 1:600), CD11b (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... 2% (v/v) Goat-serum (Biolegend) and 0.05% (v/v ...
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bioRxiv - Immunology 2019Quote: ... Serum IgE was assessed by ELISA (BioLegend). To deplete CD4+ T cells ...
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bioRxiv - Microbiology 2020Quote: ... blocked with 2% Normal Serum Block (BioLegend), 1% BSA ...
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bioRxiv - Immunology 2021Quote: ... Serum IgE concentrations were measured using an ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Serum cytokines were analyzed using Legendplex Mouse Inflammation Panel (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... containing 10% blocking agent (Normal Serum Block, CAT# 927502, Biolegend) in 0.1 M PBS with 0.5% Triton X-100 for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... serum was subjected to the LEGENDplex Mouse Inflammation Panel assay (BioLegend). Technical duplicates were averaged and compared to a standard curve according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 4 % goat serum and 1:300-diluted TruStain FcX™ (Biolegend) for 1 h at RT in a humidified dark chamber ...
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bioRxiv - Immunology 2023Quote: ... Serum samples were diluted 1:5 in ELISA diluent buffer (Biolegend).
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bioRxiv - Immunology 2021Quote: ... Serum concentrations of IFNγ were measured using ELISA MAX Standard set (Biolegend), following the manufacturer’s instructions.
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bioRxiv - Physiology 2019Quote: ... blocked with mouse serum and anti-murine CD16/32 (clone 2.4G2, Biolegend) and stained for cell surface markers (See Supplemental Table 1 for list of antibodies used) ...
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bioRxiv - Immunology 2019Quote: ... blocked with 10% mouse serum and 1% Fc block (CD16/32, BioLegend), then stained with polyclonal rabbit antibody to MHV68 (1:1000)3,4 ...
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bioRxiv - Immunology 2019Quote: ... blocked with mouse serum and anti-murine CD16/32 (clone 2.4G2, Biolegend) and stained for cell surface markers (See Supplementary Table 2 for list of antibodies used) ...
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bioRxiv - Immunology 2021Quote: ... blocked with 10% mouse serum and 1% Fc block (CD16/32, BioLegend), then stained with polyclonal rabbit antibody to MHV68 (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Serum cytokines were measured using the LEGENDplex Mouse Inflammation Panel (740446, BioLegend) bead-based immunoassay ...
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bioRxiv - Cell Biology 2023Quote: Serum samples were analyzed using a bead-based multiplex assay (BioLegend, LEGENDplex, 740809) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with pre-warmed serum-free media and TGF-β (Biolegend) was added in a final concentration of 1 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... normal serum and primary antibodies specific for Asyn (Clone 4B12; Biolegend Cat#807801), pSer129 Asyn (Clone EP1536Y ...
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bioRxiv - Immunology 2022Quote: ... Tissue and serum samples were loaded directly onto ELISA plates according to protocol (Biolegend 439407 Legend Max Mouse IFN-B ELISA kit) ...
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bioRxiv - Microbiology 2020Quote: ... and probed with rabbit polyconal α-LuxR serum or mouse monoclonal α-RpoA (Biolegend) primary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Serum IL-17A was measured as part of a LEGENDPlex Multiplex protein assay (Biolegend) following the manufacturer’s protocol.