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Citations for BioLegend :
1 - 50 of 5062 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were incubated with Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) for 4 hrs or Cell Activation Cocktail (Biolegend) in the presence of Brefeldin A and Monensin for 5 hrs before staining.
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bioRxiv - Genomics 2022Quote: ... Cells were stained for the respective cell surface protein for 30 min at 4°C and measured by fluorescence-activated cell sorting (Sony SH800) (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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bioRxiv - Immunology 2023Quote: ... Soluble peptide:IAg7 proteins were produced using S2 insect cells as previously described and made into tetramers by conjugating the soluble peptide:IAg7 molecules with BV421 (BioLegend), PE ...
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bioRxiv - Cancer Biology 2023Quote: ... and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL, BioLegend). After 4 days ...
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bioRxiv - Immunology 2020Quote: The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ...
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bioRxiv - Molecular Biology 2022Quote: ... Human Anti-Virus Response Panel (Biolegend, San Diego, USA). The assay was performed in a V-bottom plate according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Cell surface proteins were stained for 20 min at 4 °C with the following antibodies: CD45.1 (BioLegend, 110714, Clone ID: A20, RRID:AB_313503), CD45.2 (BioLegend ...
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bioRxiv - Immunology 2022Quote: Human anti-virus response panel (13-plex, LEGENDplex™, BioLegend) was used to determine the protein levels of IL-1β ...
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bioRxiv - Immunology 2020Quote: Human anti-virus response panel (13-plex, LEGENDplex™, BioLegend) was used to determine the protein levels of IL-1β ...
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bioRxiv - Immunology 2024Quote: ... IFN-γ (LegendPlex Human anti-virus response panel, Biolegend #740390) and IL-8 ...
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bioRxiv - Genomics 2019Quote: ... or CC2C6 (for pyro-Glu modified protein; BioLegend, Cat. #323123/4, RRID: AB_2716202/3) anti-CD47 antibodies were used with Protein-G DynabeadsTM or Biotin-binder DynabeadsTM ...
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bioRxiv - Biochemistry 2019Quote: ... After transfection cell supernatants were collected and subjected to cytokine production analysis using LEGENDplex™ Mouse Anti-Virus Response Panel (13-plex) (BioLegend) on BD FACS Canto II flow cytometer using BD FACSDiva 8.0 software for acquisition and BioLegend’s LEGENDplex™ Data Analysis Software for data analysis.
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bioRxiv - Microbiology 2020Quote: ... using LEGENDplex bead-based immunoassays (BioLegend, human anti-virus response panel), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... and vesicular stomatitis virus (VSV) tag (Biolegend UK Ltd, London, UK) followed by the appropriate secondary peroxidase-conjugated antibody (Sigma ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: The Human Anti-Virus Response Panel (13-plex; BioLegend, San Diego, CA) was used to quantitate human plasma cytokines (IL-1β ...
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bioRxiv - Immunology 2022Quote: ... Cell surface expression of CTLA-4 was measured by adding anti-CTLA-4-PE-Dazzle594 mAb (BioLegend) in culture during restimulation for 24 h ...
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bioRxiv - Cell Biology 2023Quote: Apical supernatant from cells at each harvest time point was assessed using LEGENDPlex Human Anti-Virus Response Panel (BioLegend, San Diego, CA, USA), as per the manufacturer’s instructions and using a FACSCanto II flow cytometer (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated for 4 hours with Brefeldin A (Biolegend) and Monensin (Sigma Aldrich) ...
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bioRxiv - Microbiology 2021Quote: Cytokines were measured using the LEGENDplex™ Human Anti-Virus Response Panel (BioLegend). The kit was used according to manufacturer’s manual with an additional fixing step ...
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bioRxiv - Cancer Biology 2022Quote: ... we stained the cells with 4 μg/ml PE-streptavidin (BioLegend) at the 37 °C for 45 min ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde (Biolegend, San Diego, CA, USA) for 10 minutes and subsequently permeabilized with 0.1% Triton X100 (Merck KGaA ...
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bioRxiv - Immunology 2023Quote: ... after stimulating cells for 4 hours with Brefeldin A (Biolegend #420601). Absolute cell counts were determined using counting beads (Biolegend #424902) ...
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bioRxiv - Immunology 2023Quote: ... after stimulating cells for 4 hours with Brefeldin A (Biolegend #420601). Absolute cell counts were determined using counting beads (Biolegend #424902) ...
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bioRxiv - Microbiology 2021Quote: ... or Lassa virus (LASV) GPC peptide pool together with 5μg/ml Brefeldin A (Biolegend). Following surface staining with Live/Dead-APC/Cy7 ...
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bioRxiv - Microbiology 2021Quote: ... or Lassa virus (LASV) GP peptide pool together with 5μg/ml Brefeldin A (Biolegend). Following surface staining with Live/Dead-APCCy7 ...
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bioRxiv - Genomics 2019Quote: ... cells were stained in batches of 1×107 cells for 30 min at 4°C (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed for 20 min with 4% paraformaldehyde (Fixation buffer; BioLegend). When intracellular staining was performed ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained at 4 °C with Zombie Violet staining (BioLegend, 423113) to exclude dead cells ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% paraformaldhyde and permeabilised with CytoPerm buffer (Biolegend). Cells were analysed on BD LSR Fortessa X-20 cytometer ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were treated with TNF-α recombinant human protein (Biolegend) at a concentration of 75 ng/ml for 24 h in combination with cycloheximide (CHX ...
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bioRxiv - Genomics 2021Quote: ... Cells sorted for CTLA-4 were treated with Cell Activation Cocktail without Brefeldin A (Biolegend, Cat #423302) for 4 hours prior to fixation ...
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bioRxiv - Cancer Biology 2020Quote: ... Other cytokines were assayed using the LEGENDPlex multiplex assay (Human Anti-Virus Response Panel, BioLegend). Fluorescence was analyzed using a FACS Canto II (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... Samples were thawed and used undiluted in LEGENDplex™ Human Anti-Virus Response Panel (Biolegend) according to manufacturer’s instructions to measure different cytokines ...
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bioRxiv - Microbiology 2022Quote: ... a LegendPlex Human Anti-Virus Response Panel were used according to the manufacturer’s directions (BioLegend). Briefly ...
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bioRxiv - Immunology 2021Quote: ... Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
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bioRxiv - Immunology 2020Quote: ... the biotinylated proteins were incubated for a minimum of 1 h at 4°C with the streptavidin-conjugates AF647 (Biolegend), BV421 (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then fixed in 4% paraformaldehyde and permeabilized using Permeabilization buffer (Biolegend). Intracellular proteins were stained with antibodies against TNF (MP6-XT22 ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed for 10 min at 4°C using IC fix (Biolegend) and cells were then incubated in for 20 min at RT in Permeabilization buffer (biolegend) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and stained with CTLA-4-PE-Dazzle 594 (Biolegend), Ki67-PerCP-eFluor 710 (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... The sorted cells were centrifuged at 400xg for 5mins at 4°C and re-suspended in 25μl Cell Staining Buffer (BioLegend). 2.5μl of 1:10 TruStain FcX™ PLUS (antimouse CD16/32 ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Genomics 2022Quote: ... at 4°C for 25 minutes and washed thrice with Cell Staining Buffer (BioLegend) and pooled equally as described previously18 ...
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bioRxiv - Immunology 2022Quote: ... cells were re-stimulated for 4 hr with PMA/Ionomycin and Brefeldin A (Biolegend), according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... After 4 days of co-culture cells were collected stained for CD8-PECy5 (BioLegend) and assessed for cell proliferation with flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Cells were placed in 4 ml of 1X annexin binding buffer (Biolegend, cat#: 422201) with biotinylated annexin V (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... The upper phase of plasma was saved and subjected to LEGENDplex Anti-Virus Response Panel (740446) (BioLegend) or LEGENDplex Mouse Inflammation Panel (740446 ...
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bioRxiv - Immunology 2022Quote: ... Antibodies with specificity for the following cell surface proteins were purchased from Biolegend with different fluorochrome labels ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained with antibodies against surface proteins CD3 (APC-fire, BioLegend), CD11b (APC Cy7 ...