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Citations for BioLegend :
1 - 50 of 9737 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Soluble peptide:IAg7 proteins were produced using S2 insect cells as previously described and made into tetramers by conjugating the soluble peptide:IAg7 molecules with BV421 (BioLegend), PE ...
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bioRxiv - Immunology 2022Quote: ... Bound virus was detected with rabbit polyclonal VP1 antibody (1:10000) followed by an anti-rabbit secondary (Biolegend, 1:200). Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ...
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bioRxiv - Molecular Biology 2022Quote: ... Human Anti-Virus Response Panel (Biolegend, San Diego, USA). The assay was performed in a V-bottom plate according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Human anti-virus response panel (13-plex, LEGENDplex™, BioLegend) was used to determine the protein levels of IL-1β ...
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bioRxiv - Immunology 2020Quote: Human anti-virus response panel (13-plex, LEGENDplex™, BioLegend) was used to determine the protein levels of IL-1β ...
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bioRxiv - Immunology 2024Quote: ... IFN-γ (LegendPlex Human anti-virus response panel, Biolegend #740390) and IL-8 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Biochemistry 2019Quote: ... After transfection cell supernatants were collected and subjected to cytokine production analysis using LEGENDplex™ Mouse Anti-Virus Response Panel (13-plex) (BioLegend) on BD FACS Canto II flow cytometer using BD FACSDiva 8.0 software for acquisition and BioLegend’s LEGENDplex™ Data Analysis Software for data analysis.
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bioRxiv - Microbiology 2020Quote: ... using LEGENDplex bead-based immunoassays (BioLegend, human anti-virus response panel), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... and vesicular stomatitis virus (VSV) tag (Biolegend UK Ltd, London, UK) followed by the appropriate secondary peroxidase-conjugated antibody (Sigma ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: The Human Anti-Virus Response Panel (13-plex; BioLegend, San Diego, CA) was used to quantitate human plasma cytokines (IL-1β ...
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bioRxiv - Cell Biology 2023Quote: Apical supernatant from cells at each harvest time point was assessed using LEGENDPlex Human Anti-Virus Response Panel (BioLegend, San Diego, CA, USA), as per the manufacturer’s instructions and using a FACSCanto II flow cytometer (BD Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend, 101251, clone M1/70), CD45-Percp-Cy5.5 (BioLegend ...
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bioRxiv - Genomics 2021Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend 101251, clone M1/70), CD45-APC (BioLegend 103112 ...
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bioRxiv - Microbiology 2021Quote: Cytokines were measured using the LEGENDplex™ Human Anti-Virus Response Panel (BioLegend). The kit was used according to manufacturer’s manual with an additional fixing step ...
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bioRxiv - Microbiology 2021Quote: ... or Lassa virus (LASV) GPC peptide pool together with 5μg/ml Brefeldin A (Biolegend). Following surface staining with Live/Dead-APC/Cy7 ...
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bioRxiv - Microbiology 2021Quote: ... or Lassa virus (LASV) GP peptide pool together with 5μg/ml Brefeldin A (Biolegend). Following surface staining with Live/Dead-APCCy7 ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were treated with TNF-α recombinant human protein (Biolegend) at a concentration of 75 ng/ml for 24 h in combination with cycloheximide (CHX ...
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bioRxiv - Cancer Biology 2020Quote: ... Other cytokines were assayed using the LEGENDPlex multiplex assay (Human Anti-Virus Response Panel, BioLegend). Fluorescence was analyzed using a FACS Canto II (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... Samples were thawed and used undiluted in LEGENDplex™ Human Anti-Virus Response Panel (Biolegend) according to manufacturer’s instructions to measure different cytokines ...
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bioRxiv - Microbiology 2022Quote: ... a LegendPlex Human Anti-Virus Response Panel were used according to the manufacturer’s directions (BioLegend). Briefly ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... p38 mitogen-activated protein kinase (p38MAPK, mouse, 1:2000, Biolegend 602651), and phosphorylated-p38MAPK (phospho-p38MAPK ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) for 4 hrs or Cell Activation Cocktail (Biolegend) in the presence of Brefeldin A and Monensin for 5 hrs before staining.
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bioRxiv - Immunology 2023Quote: ... 1X protein transport inhibitor (Fisher Healthcare) and 1:1000 PMA/Ionomycin (Biolegend) were added ...
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bioRxiv - Immunology 2020Quote: ... The upper phase of plasma was saved and subjected to LEGENDplex Anti-Virus Response Panel (740446) (BioLegend) or LEGENDplex Mouse Inflammation Panel (740446 ...
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bioRxiv - Immunology 2022Quote: ... Antibodies with specificity for the following cell surface proteins were purchased from Biolegend with different fluorochrome labels ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained with antibodies against surface proteins CD3 (APC-fire, BioLegend), CD11b (APC Cy7 ...
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bioRxiv - Immunology 2023Quote: ... whereas ADT libraries (cell surface protein libraries) were processed following instructions from Biolegend. 3’ gene expression and ADT libraries were quantified by fragment analyzer and pooled for multiplexed sequencing on a NovaSeq 6000 instrument by the Deep-sequencing Facility at the Max Planck Institute for Immunobiology and Epigenetics ...
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bioRxiv - Immunology 2024Quote: ... cells were first stimulated with cell activation cocktail (1:500 or 1:2500) (Biolegend, 423301), BFA (5 mg/mL ...
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bioRxiv - Immunology 2022Quote: ... Antibodies to detect immunoprecipitated proteins were as follows: Eos (1:500, Biolegend, W15169A), STAT5 (1:5000 ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were quantified by a fluorescence-encoded bead-based multiplex assay (LEGENDplex Mouse Anti-Virus Response Panel, BioLegend), per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cells were than stained with Siglec-5 Fc chimeric protein (Biolegend, San Diego, CA) pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend ...
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bioRxiv - Genomics 2022Quote: ... Cells were stained for the respective cell surface protein for 30 min at 4°C and measured by fluorescence-activated cell sorting (Sony SH800) (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were analyzed using the LEGENDplex™ Human Anti-Virus Response Panel multiplex assay (BioLegend, San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend). PBMC were washed in cold PBS and seeded at 1 × 106 cells/well in 96-well round bottom plates and with combinations of RBD and decoy tetramers by incubation for 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend).
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-NPC proteins (FXFG repeat-containing nucleoporins) (1:1,000; mAb414; 902907, Biolegend), rabbit monoclonal anti-BANF1/BAF (1:200 ...
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bioRxiv - Immunology 2021Quote: ... and mouse anti-β-tubulin (1:1000; BioLegend; used as a control loading protein). Membranes were then incubated with appropriate secondary antibody (1:5000 ...
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bioRxiv - Molecular Biology 2022Quote: ... and probed with protein-specific antisera against RpoB (1:10,000 dilution, BioLegend, cat# 663905), 3xFLAG (1:1,000 dilution ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... Proteins were detected with monoclonal antibodies against ubiquitin (BioLegend Cat. No. 646304; 1:1000), human HIF-1α (#610959 ...
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bioRxiv - Immunology 2024Quote: ... Purified mouse anti-SARS-CoV-2 spike protein S2 antibody (1:3000 dilution, BioLegend) and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with 1:1000 diluted Cell Stimulation cocktail (Biolegend) overnight at 37°C ...
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bioRxiv - Immunology 2023Quote: ... HTO stained PBMCs from all 10 donors were then pooled and stained with oligo-tagged TotalSeq C antibody for cell surface proteins for T cell memory markers (CD45RA, CCR7, CD95 and IL7Rα) (0.5 µg/condition; Biolegend, Table S2B) for 30 minutes on ice ...
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Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairmentbioRxiv - Molecular Biology 2022Quote: Synchronized EL4 cells were stimulated by Ig Protein with anti-CD3 (final 2μg/ml, Biolegend) conjugated magnetic beads ...
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bioRxiv - Immunology 2024Quote: ... and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution) or a purified rat anti-SARS-CoV-2 spike protein S1 antibody (1:1000 dilution, BioLegend) and HRP-conjugated goat anti-rat secondary antibody (1:3000 dilution ...
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bioRxiv - Immunology 2021Quote: Paired maternal and infant plasma were thawed and tested in the 13-plex LegendPlex Human Anti-Virus Response Panel kit (Biolegend), to quantify levels of IL-1β ...
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bioRxiv - Immunology 2022Quote: ... a multiplex cytokine quantification (LEGENDplex HU Anti-Virus Response Panel (13-plex) and LEGENDplex HU Th Cytokine Panel (13-plex) (cat# 740390 and cat# 740722, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and was analyzed with the DropArray (Curiox Biosystems) and the MAGPIX System (Luminex) or with LEGENDPlex Human Anti-Virus Response Panel (13-plex, BioLegend) and was analyzed with LSRII (BD ...