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Citations for BioLegend :
1 - 50 of 2643 citations for Coagulation factor IX F9 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... GFP (clone 1GFP63, BioLegend or clone 9F9.F9, Rockland) and mCherry (clone EPR20579 ...
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bioRxiv - Microbiology 2021Quote: ... anti-GAPDH (FF26A/F9) and anti-CRISPR Cas9 (7A9) from BioLegend; rabbit anti-HA mAb (C29F4 ...
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bioRxiv - Immunology 2022Quote: ... and recombinant human transforming growth factor beta-1 (TGF-β1; Biolegend) were used at final concentrations of 10 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... and 200 ng/mL recombinant human B cell-activating factor (BAFF, BioLegend).
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bioRxiv - Neuroscience 2021Quote: The LEGENDplexTM Human Inflammation (#740809) and Growth Factor (#740180) kits (BioLegend, CA, USA) were used to detect cytokines and growth factors in conditioned media from 3D MDMi and ReNcell VM mono- and co-cultures ...
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bioRxiv - Immunology 2023Quote: ... Levels of soluble factors were quantified simultaneously using the LEGENDplex Human CD8/NK Panel (BioLegend), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Immunology 2023Quote: Antibodies for reconstitution analysis of HIS mice (all antibodies are anti-human): CD45-Pacific Blue (Biolegend, HI30, Cat. 304029), CD3-PE (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... anti-His PE (Biolegend), and human Hashtag 3 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
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bioRxiv - Physiology 2023Quote: ... zoledronic acid (Novartis Pharmaceuticals) and/or 50 μg/kg (i.p.) recombinant human granulocyte-colony stimulating factor (G-CSF; carrier-free, BioLegend #578604) for three days ...
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bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...
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bioRxiv - Microbiology 2024Quote: ... and where indicated containing 25 ng/mL human granulocyte-macrophage colony-stimulating factor (GM-CSF) ((# 572903, Biolegend, San Diego, CA) at 37 °C in a humidified chamber with 5% CO2 in 96-well ...
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bioRxiv - Immunology 2022Quote: ... Transcription factor staining was performed using True-Nuclear Transcription Factor Buffer Set (Biolegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and brain-derived neurotrophic factor (BDNF) were assessed using the Human LEGENDplex Multiplex Assay (BioLegend®) was used following manufacturer’s instructions (Lehmann et al ...
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bioRxiv - Immunology 2023Quote: ... and anti-His PE (Biolegend), at 4 °C for 1 h ...
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bioRxiv - Immunology 2024Quote: ... transcription factors and (BD, Biolegend). Data were acquired with FACSymphony (BD ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with secondary antibody (1/100 dilution of chicken anti-HIS-FITC polyclonal from Immunology Consultants Laboratory; or 1/250 anti-human IgG-APC clone HP6017 from BioLegend) for 30 minutes on ice ...
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bioRxiv - Bioengineering 2021Quote: ... The His tagged ACE2 variants were detected using anti-His mAb (Cat. No. 652505, BioLegend).
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bioRxiv - Immunology 2023Quote: ... intracellular staining for transcription factor (Foxp3) was performed on using True-Nuclear Factor kit (Biolegend) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated with M-CSF (100ng/ml) Recombinant Human M-CSF (Macrophage-Colony Stimulating Factor, BioLegend cat. 574806, San Diego, CA, USA) for 5 min ...
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bioRxiv - Bioengineering 2023Quote: ... APC-conjugated His-tag antibody (Biolegend) was added to the cells treated with no nanobody proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-His secondary antibody (Biolegend, #362607) was used to detect IgG4-His primary antibodies ...
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bioRxiv - Immunology 2022Quote: ... permeabilised and stained for transcription factors using the True-Nuclear Transcription Factor Buffer Set (Biolegend, #424401). Cytokines and transcription factors in the same FACS panel were stained with the FOXP3 transcription factor staining kit (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... and transcription factor staining kit (Biolegend). To assess the effect of RORγt inhibition on IL-17 production the specific inhibitor SR1001 (Tocris ...
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bioRxiv - Immunology 2022Quote: ... and APC anti-His Tag antibody (Biolegend) for 30 min each ...
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bioRxiv - Immunology 2019Quote: His-tagged recombinant soluble CD40L (sCD40L, BioLegend) was incubated with NTA-SUV or plain SUV ...
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bioRxiv - Cancer Biology 2022Quote: ... permeabilized and stained for transcription factors using the T rue-Nuclear Transcription Factor Buffer Set (Biolegend, Cat# 424401) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... True Nuclear Transcription Factor Buffer set (Biolegend) was used and cells were incubated with antibodies for intracellular staining during 40min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were also supplemented with a physiological growth factor cocktail consisting of 0.2 ng/ml stem cell factor (SCF, catalogue no. 573902; BioLegend), granulocyte macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged S1-RBD was detected with Alexa Fluor 488 anti-his tag antibody (clone J095G46, Biolegend, UK) 1:5000 in 25 μL PBST + 0.1% BSA per well ...
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bioRxiv - Immunology 2020Quote: ... APC-anti-his tag antibody (Biolegend, clone: J095G46)) ...
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bioRxiv - Biochemistry 2022Quote: ... and Purified anti-His Tag antibody (BioLegend 652502) for 1 hour in blocking buffer shaking at rt ...
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bioRxiv - Microbiology 2023Quote: ... Phycoerythrin (PE) anti-His tag antibody (BioLegend 362603) was added to the cell suspension at a dilution of 1:20 and incubated for 30 minutes on ice in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... and mouse anti-His (1:2500, 362601, BioLegend) were diluted in 5% BSA in 1× PBS + 0.2% Tween20 for blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 ng/mL granulocyte macrophage colony stimulating factor (GM–CSF, for M1 polarization) or 50 ng/mL macrophage colony-stimulating factor (M–CSF, for M2 polarization) (BioLegend, San Diego ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed again in FACS buffer and intracellularly stained to detect transcription factor expression using the True-Nuclear Transcription Factor Buffer Set (cat# 424401, Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The Foxp3 Transcription Factor Staining Buffer Kit (Biolegend) was used for staining GATA3 ...
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bioRxiv - Genomics 2022Quote: ... True-Nuclear Transcription factor Buffer set (Biolegend #424401) was used according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... processed using a transcription factor buffer kit (Biolegend), and subsequently stained with antibody against FOXP3 (Biolegend).
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bioRxiv - Biophysics 2019Quote: ... 10 ng/ml basic Fibroblast Growth Factor (Biolegend). All reagents were from ThermoFisher Scientific if another supplier is not stated.
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bioRxiv - Immunology 2023Quote: ... and 50 ng/mL stem cell factor (Biolegend). Bone marrow cells were transduced with retroviral supernatants supplemented with Polybrene (6 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... True-Nuclear Transcription Factor Staining Buffer Set (Biolegend); Fixable Viability Dye (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... and True-Nuclear Transcription Factor Buffer Set (BioLegend) was used to fix and permeabilize cells before staining with anti-FoxP3-PE (Clone REA788 ...
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bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Two microliters of mouse anti-hexa His antibody (BioLegend) were added to the 10 mL sample and incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 100μl of HRP anti-His Tag Antibody (BioLegend, USA) (diluted 1:20000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and a HRP anti-His tag antibody (Biolegend, #652504) diluted 1:1000 in Superblock (Thermo #37515 ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated anti-His tag secondary antibody (BioLegend, 652503) diluted 1:2000 in blocking buffer was then added to the plates and incubated at RT for 1 hour ...
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bioRxiv - Immunology 2022Quote: Mouse anti-His Tag antibody (clone J099B12, BioLegend #652502), goat anti-mouse IgG ...