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1 - 50 of 6203 citations for 7 Octen 1 ol since 2019

• Genomic properties of variably methylated retrotransposons in mouse

  Jessica L. Elmer, et al., bioRxiv - Genetics 2020

  Quote: ... 7-aminoactinomycin D (7-AAD) (1:100; Biolegend, 420404) was added as a viability stain ...
• Mitochondrial Complex II In Intestinal Epithelial Cells Regulates T-cell Mediated Immunopathology

  Hideaki Fujiwara, et al., bioRxiv - Immunology 2020

  Quote: ... 7-AAD (#420404, 1:100, BioLegend), Annexin V (#640947 ...
• Deconvolution Of Hematopoietic Stem/Progenitor Cell Signaling Predicts Inflammatory Niche Remodeling To Be A Determinant Of Tissue Failure And Outcome In Human AML

  Lanpeng Chen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD51-PE (1:50, RMV-7, Biolegend), Sca1-PacificBlue (1:100 ...
• NOTCH1 signaling establishes the medullary thymic epithelial cell progenitor pool during mouse fetal development

  Jie Li, et al., bioRxiv - Developmental Biology 2019

  Quote: ... or CD44-PerCP (BioLegend, IM-7, 1:150), at 4°C for 30□min ...
• Systemic Lupus Erythematosus Serum Stimulation of Human Intestinal Organoids Induces Changes in Goblet Cell Differentiation and Mitochondrial Fitness

  Inga Viktoria Hensel, et al., bioRxiv - Cell Biology 2023

  Quote: ... Viability dye 7-AAD (1:250; BioLegend, 420403) was added for 10 min at RT ...
• Activated interstitial macrophages are a predominant target of viral takeover and focus of inflammation in COVID-19 initiation in human lung

  Timothy Ting-Hsuan Wu, et al., bioRxiv - Genomics 2023

  Quote: ... CD169/Siglec-1 (Clone 7-239, Biolegend 346002), or CD209/DC-SIGN (Clone 120507 ...
• Detection of tumor-derived extracellular vesicles interactions with immune cells is dependent on EV-labelling methods

  Luisa Loconte, et al., bioRxiv - Cell Biology 2023

  Quote: ... Pecy-7 (Biolegend, clone HCD14, diluted 1/100); anti-CD16 ...
• Group A Streptococcus strains causing meningitis without distinct invasive phenotype

  Laura Marquardt, et al., bioRxiv - Microbiology 2023

  Quote: ... and 7 AAD (#420404; Biolegend 1:25 dilution) diluted in Annexin V binding buffer ...
• Levamisole suppresses activation and proliferation of human T cells by the induction of a p53-dependent DNA damage response

  Gerarda H. Khan, et al., bioRxiv - Immunology 2023

  Quote: ... 7-aminoactinomycin-D (7-AAD; BioLegend), FAS-PEcy7 (DX2 ...
• Fatty acid amide hydrolase drives adult mammary gland development by promoting luminal cell differentiation

  Isabel Tundidor, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 7-aminoactinomycin D (7-AAD) (Biolegend) was used as death cell marker and added 15’ before the analysis ...
• High Frequencies of PD-1⁺TIM3⁺TIGIT⁺CTLA4⁺ Functionally Exhausted SARS-CoV-2-Specific CD4⁺ and CD8⁺ T Cells Associated with Severe Disease in Critically ill COVID-19 Patients

  Pierre-Gregoire Coulon, et al., bioRxiv - Immunology 2022

  Quote: ... anti-CD138 (APC-Cy-7, clone 4B4-1 – BioLegend) and anti-CD134 (BV650 ...
• A bivalent ChAd nasal vaccine protects against SARS-CoV-2 BQ.1.1 and XBB.1.5 infection and disease in mice and hamsters

  Baoling Ying, et al., bioRxiv - Microbiology 2023

  Quote: ... CD44-PE/Cyanine 7 (1:100, clone: IM7, BioLegend), CD69-FITC (1:100 ...
• Single-cell analysis of bidirectional reprogramming between early embryonic states reveals mechanisms of differential lineage plasticities

  Vidur Garg, et al., bioRxiv - Developmental Biology 2023

  Quote: ... or 7-AAD viability dye (BioLegend; 1 µl/sample). The following antibodies were used per 100 µl of staining volume for 1×106 cells ...
• A Broad-Spectrum Multi-Antigen mRNA/LNP-Based Pan-Coronavirus Vaccine Induced Potent Cross-Protective Immunity Against Infection and Disease Caused by Highly Pathogenic and Heavily Spike-Mutated SARS-CoV-2 Variants of Concern in the Syrian Hamster Model

  Swayam Prakash, et al., bioRxiv - Immunology 2024

  Quote: ... anti-CD137 (APC-Cy-7, clone 4B4-1 – BioLegend) and anti-CD134 (BV650 ...
• Constraint-based modeling identifies metabolic vulnerabilities during the epithelial to mesenchymal transition

  Scott Campit, et al., bioRxiv - Cancer Biology 2022

  Quote: ... 1) AnnexinV/7-AAD staining (Kit from Biolegend Cat# 640922): At the end of the EMT experiment described above ...
• Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD38 BV510 (1:50 dilution, clone HB-7, BioLegend), anti-CD20 PE-Cy7 (1:50 dilution ...
• Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD38 BV421 (1:100 dilution, clone HB-7, BioLegend), anti-CD20 BV510 (1:50 dilution ...
• Mutability and hypermutation antagonize immunoglobulin codon optimality

  Joshua J.C. McGrath, et al., bioRxiv - Immunology 2024

  Quote: ... cells were incubated with 7-AAD (Biolegend #420404, 1:20). Total B cells were identified as DAPI−CD3−B220+ and sorted using a BD FACS Melody ...
• Antibody-drug conjugates targeting CD45 plus Janus kinase inhibitors effectively condition for allogeneic hematopoietic stem cell transplantation

  Stephen P. Persaud, et al., bioRxiv - Immunology 2020

  Quote: ... 7-aminoactinomycin D (7-AAD; BioLegend, San Diego, CA) was added at 1 μg/mL immediately before analysis ...
• IL-15 superagonist N-803 enhances IFNγ production and alters the trafficking of MAIT cells in SIV+ macaques

  Amy L. Ellis-Connell, et al., bioRxiv - Immunology 2022

  Quote: ... recombinant IL-7 (rhIL-7; Biolegend; San Diego, CA), or media control ...
• Influenza A Virus Exacerbates Group A Streptococcus Infection and Thwarts Anti-bacterial Inflammatory Responses in Murine Macrophages

  Johann Aleith, et al., bioRxiv - Immunology 2022

  Quote: ... suspended in RB and 7-Aminoactinomycin (7-AAD, Biolegend) was added at a concentration of 1.25 μg/mL for at least 5 min prior to measurement.
• Siglec-1 on dendritic cells mediates SARS-CoV-2 trans-infection of target cells while on macrophages triggers proinflammatory responses

  Daniel Perez-Zsolt, et al., bioRxiv - Microbiology 2021

  Quote: ... and an anti-Siglec-1 7-239 Alexa 647 mAb (Biolegend). Cells were cytospun into coverslips ...
• Mutated clones driving leukemic transformation are already detectable at the single cell level in CD34-positive cells in the chronic phase of primary myelofibrosis

  Sandra Parenti, et al., bioRxiv - Cancer Biology 2020

  Quote: ... CD38 (HB-7, PE-Cy7, BioLegend, Cat no. 356608, 1:80) and HLA-DR (L243 ...
• Prime-pull immunization of mice with a BcfA-adjuvanted vaccine elicits mucosal immunity and prevents SARS CoV-2 infection and pathology

  Mohamed M. Shamseldin, et al., bioRxiv - Immunology 2022

  Quote: ... GL-7: PerCP-Cy5.5 (1:200; clone: 1D3, BioLegend Cat.152410); Fas-BV421 (1:300 ...
• IL-15 enhances anti-tumor immunity of sipuleucel-T by activating lymphocyte subsets and reversing immunoresistance

  Muhammad A. Saeed, et al., bioRxiv - Immunology 2023

  Quote: ... Samples were stained with 7-AAD (5 μL/1 × 106, BioLegend) to identify dead cells ...
• Short-term oxaliplatin exposure according to established hyperthermic intraperitoneal chemotherapy (HIPEC) protocols lacks effectiveness in vitro and ex vivo

  Markus W. Löffler, et al., bioRxiv - Pharmacology and Toxicology 2019

  Quote: ... 7-amino-actinomycin D viability staining solution (7-AAD; BioLegend) was added to each well at a final concentration of 600 ng/ml ...
• Expression of inhibitory checkpoint ligands by Glioblastoma Multiforme cells and the implications of an enhanced stem cell-like phenotype

  Laverne D Robilliard, et al., bioRxiv - Cell Biology 2019

  Quote: ... 7-AAD (BioLegend) was used for live/dead discrimination ...
• Mucin O-glycans facilitate symbiosynthesis to maintain gut immune homeostasis

  Takahiro Yamada, et al., bioRxiv - Microbiology 2019

  Quote: ... 7-AAD (BioLegend) was added to the cell suspension to label any dead cells ...
• Anti-apoptotic clone 11 derived peptides induce in vitro death of CD4+ T cells susceptible to HIV-1 infection

  Anastassia Mikhailova, et al., bioRxiv - Microbiology 2020

  Quote: ... 7-AAD (Biolegend) was added for the last 5 minutes of incubation ...
• Brain injury accelerates the onset of a reversible age-related microglial phenotype associated with hyperphagocytosis and inflammatory neurodegeneration

  Rodney M. Ritzel, et al., bioRxiv - Neuroscience 2022

  Quote: ... 7-AAD (Biolegend), was diluted at 5uL in 100uL sample ...
• Quantitative classification of chromatin dynamics reveals regulators of intestinal stem cell differentiation

  Jesse R Raab, et al., bioRxiv - Genomics 2019

  Quote: ... 7-AAD (Biolegend) and Annexin V-APC (Biolegend ...
• A FRET biosensor, SMART, monitors necroptosis in renal tubular epithelial cells in a cisplatin-induced kidney injury model

  Shin Murai, et al., bioRxiv - Cell Biology 2022

  Quote: ... phycoerythrin (PE) cyanine-7–conjugated anti-CD11b (101215, Biolegend, diluted 1:100), PE-conjugated anti-F4/80 (50-4801 ...
• Chronic ethanol drinking in non-human primates induces inflammatory cathepsin gene expression in alveolar macrophages accompanied by functional defects

  Sloan A. Lewis, et al., bioRxiv - Immunology 2021

  Quote: ... CD169 (Biolegend, 7-239), HLA-DR (Biolegend ...
• ERK3-MK5 signaling regulates myogenic differentiation and muscle regeneration by promoting FoxO3 degradation

  Mathilde Soulez, et al., bioRxiv - Cell Biology 2021

  Quote: ... and 7-AAD (Biolegend). Satellite cells were isolated by FACS using a gating strategy gated based on forward scatter (FSC ...
• Systemic immune dysfunction in cancer patients driven by IL6 and IL8 induction of an inhibitory receptor module in peripheral CD8⁺ T cells

  Ashwin Somasundaram, et al., bioRxiv - Cancer Biology 2020

  Quote: ... CD38 (HB-7; Biolegend), HLA-DR (L243 ...
• Optimized CRISPR-mediated gene knock-in reveals FOXP3-independent control of human Treg identity

  Avery J. Lam, et al., bioRxiv - Immunology 2021

  Quote: ... or 7-AAD (BioLegend) was used to exclude dead cells ...
• The CARD8 inflammasome drives CD4⁺ T-cell depletion in HIV-1 infection

  Qiankun Wang, Liang Shan, bioRxiv - Microbiology 2023

  Quote: ... IL-7 (BioLegend #581904), IL-15 (BioLegend #570306) ...
• Tumor-resident regulatory T cells in pancreatic cancer express the αvβ5 integrin as a targetable activation marker

  Kodai Suzuki, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and 7-AAD (BioLegend) for 15 min at room temperature to detect apoptotic cells by flow cytometry.
• Transcriptome analyses of mouse cardiac myocytes and non-cardiomyocytes: postmitotic vs. proliferative cells

  Yasuhiro Takenaka, et al., bioRxiv - Physiology 2023

  Quote: ... 7-AAD (BioLegend, #420403) was used to gate live cells ...
• CD4 T cells and neutrophils contribute to epithelial-mesenchymal transition in breast cancer

  Amélien Sanlaville, et al., bioRxiv - Cancer Biology 2023

  Quote: ... CD90.1 (OX-7; Biolegend) and CD105 (MJ7/18 ...
• Selective advantage of mutant stem cells in clonal hematopoiesis occurs by attenuating the deleterious effects of inflammation and aging

  Niels Asger Jakobsen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and 7-AAD (Biolegend) was used for dead cell exclusion ...
• The ICF2 gene Zbtb24 specifically regulates the differentiation of B1 cells via promoting heme synthesis

  Jun Wang, et al., bioRxiv - Immunology 2024

  Quote: ... Sometimes 7-AAD (Biolegend) and NP-Ficoll-FITC (NP-FITC ...
• Human skeletal muscle ageing atlas

  Veronika R. Kedlian, et al., bioRxiv - Genomics 2022

  Quote: ... anti-human CD82-PE/Cyanine 7 monoclonal antibody (BioLegend, #342109, 1:500 dilution) and anti-human CD56-PE/Cyanine 7 monoclonal antibody (eBioscience ...
• Lineage plasticity enables low-ER luminal tumors to evolve and gain basal-like traits

  Gadisti Aisha Mohamed, et al., bioRxiv - Cancer Biology 2022

  Quote: ... PE/Cyanine 7 anti-mouse CD31antibody (Biolegend, 102418, clone:390, 1:100 dilution), and PE/Cyanine 7 anti- mouse CD45 antibody (Biolegend ...
• Nucleoporin93 (Nup93) Limits Yap Activity to Prevent Endothelial Cell Senescence

  Tung D. Nguyen, et al., bioRxiv - Physiology 2023

  Quote: ... Cell suspensions were additionally incubated with 7-AAD Solution (1:100, BioLegend, 420404) for 5 minutes to detect dead cells and passed through cell strainer tubes (Falcon ...
• E protein control of NKγδT cell development through both generation and function of the stereotypic Vγ1Vδ6.3 TCR

  Ariana Mihai, et al., bioRxiv - Immunology 2024

  Quote: ... and only in the presence of 1 ng/mL IL-7 thereafter (BioLegend). Cultures were analyzed and passaged every 3 days.
• The lysosomal TRPML1 channel promotes breast cancer survival by supporting mitochondrial function and cellular metabolism

  Shekoufeh Almasi, et al., bioRxiv - Cancer Biology 2020

  Quote: ... and 20 μg/mL 7-AAD solution (7-amino-actinomycin D, Biolegend, 650 nm) for 15 min at room temperature in dark ...
• An Immunological Synapse Formation Between T Regulatory Cells and Cancer-Associated Fibroblasts Promotes Tumor Development

  Athina Varveri, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Apoptotic B16.F10 cells were stained with 7-Amino-actinomycin D (7-AAD, Biolegend) to determine viability ...
• CD8+ lymphocytes modulate Zika virus dynamics and tissue dissemination and orchestrate antiviral immunity

  Blake Schouest, et al., bioRxiv - Immunology 2019

  Quote: ... CD169 (PE; 7-239; BioLegend), CD28 (PECF594 ...
• NINJA: an inducible genetic model for creating neoantigens in vivo

  Martina Damo, et al., bioRxiv - Immunology 2020

  Quote: ... Thy1.1 (clone OX-7, BioLegend), CD8 (clone 53-6.7 ...