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Citations for BioLegend :
1 - 50 of 1817 citations for 6 Chloro 5 methoxypyridin 2 amine hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... 4′,6-diamidino-2-phenylindole (DAPI; BioLegend) was diluted 1:10,000 from a 5 mg mL-1 stock solution in deionized water ...
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bioRxiv - Neuroscience 2022Quote: ... 4’,6-diamidino-2-phenylindole (1:10,000; Biolegend) was used.
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bioRxiv - Immunology 2019Quote: ... or DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate; Biolegend). Intracellular staining was carried out using Human FoxP3 Buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Neuroscience 2020Quote: ... 6-diamidino-2-phenylindole (DAPI) (diluted 1:20’000; BioLegend, #422801). Finally ...
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bioRxiv - Neuroscience 2021Quote: ... 6- diamidino-2-phenylindole (DAPI) (diluted 1:20,000; BioLegend, #422801). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... amine-reactive resin was coupled with anti-Aβ 6E10 antibody (BioLegend, USA) followed by incubation with PBS-soluble fraction of AD brain homogenate ...
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bioRxiv - Biochemistry 2022Quote: ... and fixable live/dead stained with Zombie Red amine-reactive dye (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and FFA content were measured by ELISA (IL-1β, Biolegend, 432604; IL-6, Invitrogen, BMS603-2) or a FFA fluorometric kit (Cayman Chemical ...
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bioRxiv - Immunology 2022Quote: ... 5 ng/mL IL-2 (Biolegend 575404) was added to media and cells were cultured another 2 days (Lutz et al. ...
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bioRxiv - Cell Biology 2022Quote: ... or 2 μg ml−1 IL-6 neutralizing antibody (MP5-20F3, Biolegend) at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... and 5 ng/mL IL-2 (Biolegend 575404) at 1×107 cells/mL for 72 hrs ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with PBS and stained with an amine–reactive dye (Biolegend #423104) for 20 minutes to assess cell viability ...
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bioRxiv - Neuroscience 2023Quote: ... ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2, Biolegend, San Diego ...
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bioRxiv - Pathology 2019Quote: ... IL-9 and IL-6 determinations (Human IL-4, IL-5, IL-9 and IL-6 MAX, Biolegend, San Diego, CA 92121 USA) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with 1× PBS and stained with an amine–reactive dye (Biolegend # 423106) for 20 minutes at room temperature to assess cell viability ...
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bioRxiv - Immunology 2023Quote: ... Viability of cells was assessed via Zombie Yellow amine-reactive permeability dye (BioLegend, Cat# 423103). Data were acquired using a spectral unmixing Cytek Aurora flow cytometer (Cytek ...
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bioRxiv - Immunology 2023Quote: ... macrophages were challenged with CFSE (5-[and 6]-Carboxyfluorescein diacetate succinimidyl ester Kit, BioLegend,423801)-labeled apoptotic cells at a 4:1 (AC/Macrophage ...
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bioRxiv - Immunology 2024Quote: ... Brefeldin A (5 μg/ml) and Monensin (2 μM) (BioLegend) were added to the cultures during the last 4 h of incubation to allow intracellular cytokine accumulation ...
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bioRxiv - Cancer Biology 2019Quote: ... at concentration 2×10^6/ml in IMDM containing 10% FBS and 100U/ml of recombinant mouse IL-2 (BioLegend, CA). Cells were cultured for up to 7 days and used for functional experiments in vitro and in vivo.
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bioRxiv - Microbiology 2022Quote: ... unpulsed splenocytes and the S-peptide-pulsed splenocytes were stained with 0.5 μM and 5 μM (respectively) of 5-(and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE; BioLegend, San Diego, CA, USA). The unpulsed and peptide-pulsed splenocytes were mixed at a 1:1 ratio (5 × 106 cells each) ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were collected at 2 hours and 4 hours and analyzed using IL-6 (Biolegend). After removal of supernatant ...
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bioRxiv - Microbiology 2020Quote: ... PBS supplemented with 0.1 µg/ml antifade-4 6-diamidino-2-phenylindole (DAPI) (BioLegend, USA) was added to the cells for at least 30 min before imaging ...
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bioRxiv - Immunology 2023Quote: ... The nuclei were stained with 3 µM DAPI (4’,6-diamidino-2-phenylindole; BioLegend, #422801) for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... After that 1/1000 dilution of zombie aqua amine reactive dye (Biolegend, San Diego, CA; cat# 423101) was added to the cell suspensions and incubated for 30 minutes in the dark ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated with a Live/Dead amine reactive viability dye (Zombie ultraviolet (UV) dye) (BioLegend) diluted 1:2000 in 10 μl 1X PBS for 15 min at RT in the dark to facilitate dead cell exclusion ...
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bioRxiv - Immunology 2021Quote: ... or 5 μg/ml anti-human TRAIL antibody (RIK-2, Biolegend). After 2 h of preincubation effector cells were added to target cells.
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Cancer Biology 2022Quote: ... using ELISA MAX Deluxe Set human (IFNγ #430104, IL-2 # 431804, and IL-6 #430504, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were incubated with 4’,6-diamidino-2-phenylindole (DAPI) (422801, BioLegend, San Diego, CA) for 2 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... parasites were stained with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE; Cell Division Tracker Kit, BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Donor 5 and 6 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... and cells were instead stained with 4′,6-diamidino-2-phenylindole (DAPI; BioLegend, San Diego, CA, USA) and gated for live (DAPI- ...
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bioRxiv - Physiology 2022Quote: ... Samples were stained with 3 μM of (4’,6-Diamidino-2-Phenylindole, Dilactate (DAPI, Biolegend Cat# 422801)) ...
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bioRxiv - Immunology 2023Quote: C57BL/6 mice were treated twice with topical R848 and then were injected once I.P with 2 mg of (5-bromo-2’-deoxyuridine) BrdU (Biolegend). Bone-marrow (BM ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Microbiology 2020Quote: ... cells were counted and plated on 6 well plates in RPMI + 5% FBS + 20 ng/ml GM-CSF (BioLegend). Cells were maintained at 37° C 5% CO2 and supplemented on day 3 ...
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bioRxiv - Immunology 2019Quote: ... Approximately 106 cells/sample were re-suspended in PBS and dead cells removed using amine-reactive dye NIR Zombie (1:500 dilution in the antibody master mix; #423105, BioLegend). Live cells were stained with the following cell surface marker specific antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... IL-6 (Biolegend) and TGF-β (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... IL-6 (Biolegend) or Tocilizumab (a kind gift from Dr Ceri Roberts in Professor Leonie Taams lab ...
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bioRxiv - Microbiology 2022Quote: ... IL-6 (Biolegend), TNF-α (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... IL-6 (BioLegend® Cat ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 1 x 106 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named ‘FACS buffer’) together with 5 uL of TruStain FcX (BioLegend, 422301). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
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bioRxiv - Cancer Biology 2023Quote: 1 × 106 target cells (B16-F10 and Hepa1-6) were suspended in 1ml DPBS containing 5 μM Calcein-AM (BioLegend) and incubated at 37℃ for 30 minutes in the dark ...
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bioRxiv - Immunology 2021Quote: ... were co-incubated with 6×105 target cells expressing mCherry at 37°C for 2 hours in the presence of 2µM monensin (BioLegend). The cells were centrifuged ...
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bioRxiv - Microbiology 2019Quote: ... the splenocytes were incubated for 2 h with 5 μg/ml brefeldin A (Biolegend) for intracellular trapping of cytokines and then stained with Zombie Aqua (1:200 ...
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bioRxiv - Immunology 2019Quote: ... IL-6 was evaluated using the IL-6 DIY ELISA (Biolegend). ELISA was performed as per manufacturers instructions.
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bioRxiv - Bioengineering 2024Quote: ... Blocking occurred using 5% BSA in TBST and antibody probing using 2% BSA in TBST at the indicated dilutions: α-pERK1/2 (Biolegend 369502) 5000x ...