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Citations for BioLegend :
1 - 50 of 1613 citations for 5 TERT BUTYL 2 PROPOXY BENZENESULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 5 ng/mL IL-2 (Biolegend 575404) was added to media and cells were cultured another 2 days (Lutz et al. ...
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bioRxiv - Immunology 2022Quote: ... and 5 ng/mL IL-2 (Biolegend 575404) at 1×107 cells/mL for 72 hrs ...
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bioRxiv - Immunology 2021Quote: ... Blood samples were subjected to erythrocyte lysis with ammonium chloride (BioLegend). For harvesting bone marrow ...
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bioRxiv - Immunology 2024Quote: ... Brefeldin A (5 μg/ml) and Monensin (2 μM) (BioLegend) were added to the cultures during the last 4 h of incubation to allow intracellular cytokine accumulation ...
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bioRxiv - Immunology 2021Quote: ... or 5 μg/ml anti-human TRAIL antibody (RIK-2, Biolegend). After 2 h of preincubation effector cells were added to target cells.
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bioRxiv - Immunology 2023Quote: C57BL/6 mice were treated twice with topical R848 and then were injected once I.P with 2 mg of (5-bromo-2’-deoxyuridine) BrdU (Biolegend). Bone-marrow (BM ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 1 x 106 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named ‘FACS buffer’) together with 5 uL of TruStain FcX (BioLegend, 422301). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Microbiology 2019Quote: ... the splenocytes were incubated for 2 h with 5 μg/ml brefeldin A (Biolegend) for intracellular trapping of cytokines and then stained with Zombie Aqua (1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... Blocking occurred using 5% BSA in TBST and antibody probing using 2% BSA in TBST at the indicated dilutions: α-pERK1/2 (Biolegend 369502) 5000x ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were incubated for 5 hours with Brefeldin A (BioLegend, San Diego, California) and Monensin (BioLegend ...
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bioRxiv - Immunology 2022Quote: A 5-plex SARS-CoV-2 Variants Neutralizing Antibody Panel (BioLegend LEGENDplex, Cat. No. #741174) was used to measure the neutralization antibody concentration ...
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bioRxiv - Immunology 2022Quote: ... Omicron RBD (BA.2 or BA.5) were labeled with TotalSeq-C0971 Streptavidin (Biolegend, 405271) and TotalSeq-C0972 Streptavidin (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... Between 50,000 to 100,000 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named “FACS buffer”) together with 5 uL of TruStain FcX (BioLegend, San Diego, CA, USA). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Immunology 2021Quote: ... Anti-coagulated blood samples from recipient mice were subjected to lysis of erythrocytes with ammonium chloride (BioLegend) and then labeled at room temperature with antibodies against Ly6G and CD45.2 ...
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bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
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bioRxiv - Molecular Biology 2021Quote: ... or day 5 IL-2 cultured NK cells isolated using the MojoSort NK isolation kit from Biolegend, but instead of analysis by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Immunology 2023Quote: ... rhIL-2 (50 Uml-1, Novartis) and anti-mIFN-γ (5 µg ml-1, clone XMG1.2, Biolegend) in the presence or absence of 30 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 X10^5 cells were then resuspended to be stained with 5ul of Annexin V-FITC (#640945, Biolegend) in 100ul of Annexin V binding buffer (10mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... Red blood cells were lysed by incubation with Red Blood Cell Lysis Buffer containing ammonium chloride for 15 minutes at 4°C (BioLegend). CD34+ selection was performed using AutoMACS cell separation technology following incubation with CD34+ selection beads (Miltenyi Biotech ...
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bioRxiv - Immunology 2021Quote: ... 2.1 mmol/L ultra-glutamine in the presence of Brefeldin A (5 μg/ml) and monensin (2 μM; BioLegend). After 5 hours of incubation ...
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bioRxiv - Immunology 2021Quote: ... and stained with fluorochrome-labeled anti-mouse IFN-γ/TNF-α/IL-2/IL-4/IL-5 mAbs (Biolegend). LSRFortessa was used to acquire the flow cytometry data ...
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bioRxiv - Immunology 2022Quote: ... followed by stimulation with plate bound anti-CD3 (2 μg/ml) and anti-ICOS (5 μg/ml; C398.4A; Biolegend) for 24 hours.
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bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Immunology 2021Quote: ... 5 ng/ml IL-5 (Biolegend; 581504), and 20 ng/ml IL-2 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... streptavidin cyanine 5 (5 μg/mL, BioLegend) for 1 h ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Immunology 2023Quote: ... or phosphorylated ERK1/2 (pERK1/2; Biolegend), cells were stained with surface markers then fixed with Paraformaldehyde 1% ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 and 5 μg/mL Brefeldin A (BFA; BioLegend) was added during the last 6 h of stimulation ...
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bioRxiv - Immunology 2024Quote: ... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
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bioRxiv - Cell Biology 2021Quote: ... IL-5 (BioLegend) and IL-13 (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... IL-2 immune complex (IL-2ic; mouse recombinant IL-2 + anti-IL-2 (JES6-1A12, BioLegend); molar ratio 2:1 ...
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bioRxiv - Immunology 2019Quote: ... CD4 (RM4-5, Biolegend), CD4 (GK1.5 ...
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bioRxiv - Immunology 2022Quote: ... CD4 (RM4-5, BioLegend), CD3e (145-2C11 ...
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bioRxiv - Immunology 2020Quote: ... CD4 (RM4-5; BioLegend), CD8 (53-6.7 ...
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bioRxiv - Immunology 2021Quote: ... CD4 (RM4-5, Biolegend), CD8a (53-6.7 ...