Small ribosomal subunit protein uS12 RPS23 Antibody FITC citations for BioLegend

Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...

TRIM71 deficiency causes germ cell loss during mouse embryogenesis and promotes human male infertility

Lucia A. Torres-Fernández, et al., bioRxiv - Developmental Biology 2021

Quote: ... 106 cells were stained with 0.2 μg FITC-labelled anti-THY1.2/CD90.2 antibody (BioLegend) for 15 min at 4°C ...

Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cells

Dingxi Zhou, et al., bioRxiv - Immunology 2021

Quote: All antibodies for immunofluorescence stain are as follow: FITC-LAMP1 (121605, BioLegend, 1:100), WIPI2 (SAB4200399 ...

Spatially Resolved Measurement of Dynamic Glucose Uptake in Live Ex Vivo Tissues

Austin F. Dunn, et al., bioRxiv - Biochemistry 2020

Quote: ... Antibodies used were FITC-rat anti-mouse/human CD45R/B220 (clone RA3-6B2, BioLegend), and rat anti-mouse CD4 F(ab’)2 fragment (clone GK1.5 ...

TLR4-pathway impairs synaptic number and cerebrovascular functions through astrocyte activation following traumatic brain injury

Juliana M Rosa, et al., bioRxiv - Neuroscience 2021

Quote: ... The primary antibodies used were CD45 (Clone 30-F11, FITC, 0.6 µg/ml, Biolegend), CD11b (Clone M1/70 ...

DNA-PKcs phosphorylation at the T2609 cluster alters the repair pathway choice during immunoglobulin class switch recombination

Jennifer L. Crowe, et al., bioRxiv - Immunology 2020

Quote: ... The following antibody cocktails were used for B cell (FITC anti-mouse CD43, Biolegend, 553270 ...

A photopolymerizable hydrogel enhances intramyocardial vascular cell delivery and promotes post-myocardial infarction healing by polarizing pro-regenerative neutrophils

Xuechong Hong, et al., bioRxiv - Bioengineering 2022

Quote: ... confirmed by flow cytometry using FITC-conjugated Ly6G antibody (1:100; clone 1A8, Biolegend) 46.

Single-cell repertoire tracing identifies rituximab refractory B cells during myasthenia gravis relapses

Ruoyi Jiang, et al., bioRxiv - Immunology 2020

Quote: ... B cells were labelled with FITC-labelled anti-IgD antibody (BioLegend clone IA6-2) per manufacturer’s instructions and FITC positive populations were removed ...

Lysophosphatidylcholine acyltransferase 1 controls the mitochondrial reactive oxygen species generation and survival of the retinal photoreceptor cells

Katsuyuki Nagata, et al., bioRxiv - Biochemistry 2021

Quote: ... Photoreceptor cells were labeled with FITC anti-mouse CD73 antibody (TY/11.8, BioLegend, USA.). Dead cells were stained with LIVE/DEAD Fixable Far Red Dead Cell Stain kit (Invitrogen ...

STUB1 is an intracellular checkpoint for interferon gamma sensing

Simon Ng, et al., bioRxiv - Cancer Biology 2021

Quote: ... Primary antibodies used were: H-2Kb/H-2Db (FITC, Biolegend, #114606, 1:50 dilution), HLA-A,B,C (FITC ...

Efficient reprogramming of the heavy-chain CDR3 regions of a human antibody repertoire

Tianling Ou, et al., bioRxiv - Immunology 2021

Quote: IgM expression was detected by FITC anti-human IgM antibody (clone MHM-88, Biolegend). HA insertion was detected by APC anti-HA antibody (clone 16B12 ...

ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion

Demis Menolfi, et al., bioRxiv - Genetics 2023

Quote: ... with each antibody used at 1:200 dilution: FITC anti-mouse CD43 (Biolegend, 553270); PE anti-mouse IgM (Southern Biotech ...

Microbiota colonization tunes the antigen threshold of microbiota-specific T cell activation in the gut

Daniel Hoces, et al., bioRxiv - Immunology 2022

Quote: ... The following antibodies were used for surface and intranuclear staining: CD44-FITC (IM7, BioLegend), CD44-BV711 (IM7 ...

In vivo affinity maturation of the HIV-1 Env-binding domain of CD4

Andi Pan, et al., bioRxiv - Bioengineering 2024

Quote: ... The antibody cocktail for GC B-cell staining consisted of CD45.1-FITC (BioLegend, 110706), CD19-BV605 (BioLegend ...

PI3Kgamma promotes neutrophil extracellular trap formation by noncanonical pyroptosis in abdominal aortic aneurysm

Yacheng Xiong, et al., bioRxiv - Cell Biology 2024

Quote: ... the cell suspension was incubated with FITC-conjugated fluorescent antibody against CD11b (BioLegend, 101205) and APC-conjugated fluorescent antibody against Ly6G (BioLegend ...

Establishing a method for the cryopreservation of viable peripheral blood mononuclear cells in the International Space Station

Hiroto Ishii, et al., bioRxiv - Cell Biology 2024

Quote: Antibodies and reagents employed included FITC-anti-mouse NK1.1 (BioLegend, clone PK136, Cat#108705), PerCP-eFluor710-anti-mouse CD172a (eBioscience ...

PD-L1 blockade immunotherapy rewires cancer emergency myelopoiesis

Athina Boumpas, et al., bioRxiv - Immunology 2023

Quote: ... The following human monoclonal antibodies were used: anti-CD34-FITC (BioLegend, clone 581, # 343504), anti-CD34-APC (BD Biosciences ...

Targetable leukemia dependency on noncanonical PI3Kγ signaling

Qingyu Luo, et al., bioRxiv - Cancer Biology 2023

Quote: ... Human leukemia cells were labelled with a FITC anti-human CD45 antibody (368508; BioLegend) then sorted using a BD FACS Aria II Cell Sorter.

The mitochondrial deoxyguanosine kinase is required for female fertility in mice

Yake Gao, et al., bioRxiv - Cell Biology 2023

Quote: ... The reaction was performed with mouse anti-BrdU-FITC antibody (BioLegend Cat. No. 364103) at 4℃ overnight ...

Rational identification of potent and broad sarbecovirus-neutralizing antibody cocktails from SARS convalescents

Yunlong Cao, et al., bioRxiv - Immunology 2022

Quote: ... CD19+ B cells were then stained with FITC anti-human CD19 antibody (BioLegend, 392508), FITC anti-human CD20 antibody (BioLegend ...

Killing them softly with EPO: a new role for erythropoietin in the homeostasis of red blood cells

Clemente F. Arias, et al., bioRxiv - Physiology 2023

Quote: ... the cells were stained with F4/80 (using FITC-conjugated anti-mouse antibody, Biolegend) and SIRP-α (PE-conjugated anti-mouse antibody ...

Cross-species single-cell comparison of systemic and cardiac inflammatory responses after cardiac injury

Eric Cortada, et al., bioRxiv - Cell Biology 2023

Quote: ... The following antibodies were used for cardiac macrophages: FITC anti-CD11b (Biolegend cat. 101206), PE anti-F4/80 (Biolegend cat ...

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