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Citations for BioLegend :
101 - 150 of 1201 citations for hsa mir 33a 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with DPBS and harvested with trypsin-EDTA and the viability analyzed by flow cytometry using the Apoptosis Detection Kit with propidium iodide (PI, BioLegend, San Diego, CA, USA) in a Cytoflex flow cytometer (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: To assess cell viability human neutrophils at indicated times were washed with PBS and incubated with Zombie Aqua Fixable Viability Kit (BioLegend) for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Protein detection using anti-HA (Covance 16B12, BioLegend, Dedham, MA, USA) or antiactin antibodies (Cell Signaling ...
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bioRxiv - Microbiology 2020Quote: ... Signal detection was performed using TMB High Sensitivity Solution (BioLegend, # 421501) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Detection reagents were anti-mouse IgG-HRP (all BioLegend, London, UK). Optical densities were measured at 450 nm using a SpectraMAX i3 microplate reader (Molecular Devices ...
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bioRxiv - Immunology 2020Quote: ... Detection of biotin was performed with APC-Cy7-conjugated streptavidin (BioLegend).
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bioRxiv - Bioengineering 2020Quote: All reagents for detection of IL-2 were purchased from Biolegend. Supernatants were collected after 16 hours of coculture of 5MCAR 58α−β− cells and M12 target cells ...
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bioRxiv - Cancer Biology 2020Quote: ... BrdU-FITC antibody staining for 20 min at RT (BioLegend #364104) and 20 min incubation with PI solution (100 µg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... and double knockdown cells were treated with the IC50 value of cisplatin and viability was assessed via Annexin V and propidium iodide staining by flow cytometry (Biolegend APC Annexin V Apoptosis Detection Kit).
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bioRxiv - Neuroscience 2020Quote: ... followed by Avidin-HPR and detection with a TMB Substrate Set (BioLegend). Plates were washed 4 times with PBS containing 0.05% Tween-20 between each step.
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bioRxiv - Immunology 2021Quote: ... neutrophils were stained with an anti-CD66b Pacific Blue detection antibody (Biolegend). For detection of complement deposition ...
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bioRxiv - Immunology 2021Quote: ... neutrophils were stained with an anti-CD66b Pacific Blue detection antibody (Biolegend) prior to flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... Capture and detection antibodies were used in the following pairs from BioLegend: Il-1β (Cat # 503514 ...
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bioRxiv - Immunology 2024Quote: ... and stained for 15 min at RT with annexin V (Biolegend, 640919).
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bioRxiv - Cancer Biology 2021Quote: Vcam1 detection: APC anti-mouse CD106 (Vcam1) antibody (BioLegend, San Diego, CA, USA). Cell proliferation ...
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bioRxiv - Bioengineering 2020Quote: ... Detection of the immune cells infiltrates were performed using rat anti-mCD8 (Biolegend), rat anti-mCD4 (Biolegend) ...
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bioRxiv - Microbiology 2020Quote: ... Secondary detection used biotin-conjugated anti-mouse IgG (BioLegend #405303, 0.5 μg/ml), followed by HRP-Streptavidin conjugates (BioLegend #405210 ...
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bioRxiv - Biophysics 2020Quote: ... astrocytes were labelled 1 hour at RT with anti-GFAP (1:150,644702, BioLegend) for staining GFAP ...
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bioRxiv - Immunology 2024Quote: ... at RT with either human IgG1 isotype control (Biolegend #403502, 0.47 mg/mL), mAb C7 (0.47 mg/mL) ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 1h at RT using IC fixation/permeabilization buffer (Biolegend) and cells were then incubated for 20 min at RT in permeabilization buffer (Biolegend) ...
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bioRxiv - Microbiology 2024Quote: ... and then washed two times in FACS buffer (Biolegend). Cells were analyzed using a FACSCalibur flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: (i) Cholesterol and glycosphingolipid detection: CD4-BV711 (clone RPA-T4, BioLegend Cat# 300558, RRID:AB_2564393), CD25-BV510 (clone MA251 ...
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bioRxiv - Immunology 2020Quote: ... Detection of CD56 on cell lines was performed using clone HCD56 (1:100, Biolegend). For evaluation of cell line CD56 expression ...
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bioRxiv - Neuroscience 2019Quote: ... The following primary antibodies were used for detection: anti-FMRP (MAB2160, Millipore; 6B8, Biolegend), and anti-Calnexin (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... A biotinylated primary antibody was used for direct detection without signal amplification (Biolegend 339809). Positive staining was confirmed by reference to the testing information provided by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... Detection of Aβ species was achieved using the mouse monoclonal HRP-conjugated 6E10 (Biolegend) antibody at a dilution of 1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... with a secondary (anti-rat) detection antibody labeled with Alexa Fluor-647 (Biolegend, 407511) at a 1:500 dilution ...
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bioRxiv - Immunology 2024Quote: ... Capture and biotin conjugated detection anti-mouse IL-4 antibodies were purchased from BioLegend. Anti-mouse IL-13 antibody pair were purchased from Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for at least 60 min at RT (BioLegend, San Diego, CA, USA). Slides were washed 2 times in 0.5% BSA/PBS and once in PBS at RT ...
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bioRxiv - Immunology 2022Quote: ... we lysed red blood cells using RBC Lysis Buffer for 5min at RT (BioLegend). RBC Lysis Buffer was quenched with MACS buffer and washed once ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked for 1 hour at RT with 1x ELISA dilutant buffer (Biolegend Cat# 421203), then washed twice with 1x wash buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and DRAQ5 (1ng/mL, incubation 5 minutes at RT; BioLegend, San Diego, CA, USA). Samples were measured using a FACS Canto II (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... and cells were then incubated for 20 min at RT in permeabilization buffer (Biolegend). Intracellular staining was performed for cytokines using antibodies for IL-5 ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 times and then incubated with Avidin-HRP (Biolegend, 405103) for biotinylated probes at 1:2000 dilution for 45 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... washed for 3 times with Washing Buffer (BioLegend Cat#421601) and blocked with Dilution Buffer (0.5M Tris pH 8.0 ...
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bioRxiv - Genomics 2022Quote: ... ADT cocktails (diluted 20 times from original stock) from Biolegend were added onto the tissue and incubated for 30 minutes at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... TCR reactivity was measured through detection of CD137 surface expression (PE-anti-human CD137, Biolegend) on CD8+ TCR transduced (mTCRB+ ...
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bioRxiv - Immunology 2019Quote: For the detection of serum cytokines either the LEGENDplex Th Cytokine Panel™ from BioLegend or the BD CBA Mouse Inflammation Kit™ were used according to the manufacturers’ instructions.
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bioRxiv - Microbiology 2019Quote: ... The following antibodies were used for detection of proteins of interest: HA.11 (mouse, Biolegend), β-actin (mouse ...
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bioRxiv - Immunology 2021Quote: ... followed by addition of relevant detection mAbs: biotin anti-human IFN-γ (Clone: 4S.B3, BioLegend); biotin anti-human TNF-α (Clone ...
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bioRxiv - Immunology 2019Quote: ... at RT in the dark using the following antibodies: anti-CD3–PerCP (clone OKT3; BioLegend), anti-CD19– Brilliant Violet 510 (clone HIB19 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were collected and fixed for 20 minutes at RT with fixation buffer (Biolegend 420801), then samples were run on a BD LSRII (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... cells were assessed for viability using a 30 min RT incubation with Zombie NIR (BioLegend) diluted in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and permeabilized for 15 minutes at RT in Intracellular Staining Perm Wash Buffer (Biolegend, 421002), before staining with Recombinant anti-Cytokeratin 8 antibody Alexa Fluor 647 (Abcam ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed three times in Perm/Wash buffer (BD Biolegend), then stained for 30 minutes on ice in antibodies diluted in Perm/Wash buffer ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 4 times with washing buffer (BioLegend; Cat: 4211601) and then incubated for 1 h at RT with a 1:2000 dilution of secondary antibody of horse radish peroxidase (HRP)-conjugated rabbit anti-mouse IgM (μ chain) ...
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bioRxiv - Cancer Biology 2024Quote: MM cells were washed three times with Cell Staining Buffer (BioLegend) and resuspended in their respective cell culture media with 100 nM MitoTracker Green (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... MM cells were washed three times with Cell Staining Buffer (BioLegend) and resuspended in tetramethylrhodamine ethyl ester (TMRE ...
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bioRxiv - Cancer Biology 2024Quote: MM cells were washed three times with Cell Staining Buffer (BioLegend) and fixed in 1x Fixation Buffer (4% paraformaldehyde ...