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Citations for BioLegend :
1151 - 1200 of 1317 citations for Integrin beta 3 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines or expanded primary NK cells were stimulated with 3 ug/mL CD28 activating antibody (Biolegend, cat#302933) for 24 hours.
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper) with hashtag 3 (BioLegend, Cat# 155805); lymph node cell IL-1β/IL-23 stimulation group (LN stim ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed in HBSS with 3% BSA and incubated with CD34 Brilliant Violet 421 (BioLegend 230 119321) at 1:400 and CD24 PerCP-Cyanine 5.5 (eBioscience ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed three times in FACS buffer and resuspended in FACS buffer containing 3 μg/ml DAPI (BioLegend). Flow cytometric analysis was done on a BD LSRFortessa analyzer and sorting was done on a BD FACSAria1 machine.
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bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
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bioRxiv - Immunology 2022Quote: ... added for last 3 hrs of stimulation and stained with IFN-γ (XMG1.2; eBioscience) and IL-17A (TC11-18H10.1; Biolegend) using a Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... These cells were stimulated with 10μg/ml of anti-CD3 (OKT-3) and anti-CD28 (28.2) antibodies (Ultra-LEAF™, Biolegend) in R10-50-15 for 48 hours ...
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bioRxiv - Bioengineering 2020Quote: ... dilutions of the serum were mixed with a constant 3 nM of an HRP conjugated anti-OVA antibody (BioLegend) and incubated for 2 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Immunology 2022Quote: ... mice were injected into the retro-orbital sinus with a total of 3 mg of PE/Cy7-CD45.2 (104, BioLegend) in 200 μl sterile DPBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50,000 CAR-T cells were co-cultured with 100,000 target cells for 5h in the presence of CD107a antibody (Supp. Table 3) and 1x Brefeldin A (Biolegend). Following co-culture ...
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bioRxiv - Immunology 2024Quote: ... intestine and skin (n = 3, PCW 9.5) were stained with the following antibodies (clone, fluorophore, company): CD3 (UCHT1, FITC, Biolegend), CD7 (M-T701 ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions from 3 B16-OVA WT tumours were labelled with TotalSeq(TM)-C0301 antibody (Biolegend, Cat. 155861), and 3 IFNγRKO tumours were labelled with TotalSeq(TM)-C0302 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
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bioRxiv - Immunology 2023Quote: ... 130-120-662) for 3 days in 24-well plates coated with 3μg/ml anti-CD3 (145-2c11; Biolegend) and 3μg/ml anti-CD28 (37.51 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... For the HashTag library TotalSeq™-B0303 anti-mouse Hashtag 3 Antibody (Clone M1/42; 30-F11; 155835; BioLegend) was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42 ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit polyclonal antibody recognizing the C-terminal tail of the NMIIA heavy chain (Biolegend, 909801), rabbit polyclonal anti-fibronectin (F3648 ...
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bioRxiv - Cell Biology 2020Quote: ... a different rabbit pAb to giantin (1-469 fragment) (Poly19243) was also purchased from BioLegend Inc ...
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bioRxiv - Immunology 2021Quote: ... and then fluorochrome-labeled antibodies to surface markers and secondary antibody against rabbit IgG (Biolegend) were added ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... cell cultures were incubated with the appropriate primary antibodies (rabbit anti-Nestin, 1:500 [Biolegend #841901] ...
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bioRxiv - Cell Biology 2021Quote: ... immunoblotting was done with the following antibodies: rabbit anti-NMIIA pAb (BioLegend, #POLY19098, 1:500), rabbit anti-NMIIB pAb (BioLegend ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with the corresponding secondary antibodies: 1:500 anti-rabbit alexa 555 (Biolegend;406412) and 1:500 anti-guinea pig alexa 647 (Jackson Laboratories;706-605-148).
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bioRxiv - Cancer Biology 2020Quote: ... Beads were washed twice and incubated in PE-conjugated donkey anti-rabbit secondary antibody (Biolegend) for 30 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit anti-PAX6 (Cat#901301; RRID: AB_2565003) was purchased from BioLegend (San Diego, CA, USA). Rabbit anti-brain lipid-binding protein (BLBP ...
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bioRxiv - Developmental Biology 2022Quote: The following antibodies were used: rabbit anti-Myosin Heavy Chain 2B (1/400, Biolegend 909902), mouse anti-Myosin Heavy Chain 2B (1/50 ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then incubated with the rabbit polyclonal anti-Pax6 (1:200, BioLegend Cat # 901302) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Rabbit polyclonal antibodies to myosin IIA (19098) and myosin IIB (19099) were purchased from Biolegend. Antibodies to phospho-myosin light chain 2 (Thr18/Ser19 ...
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bioRxiv - Developmental Biology 2022Quote: ... For an IgG control we used purified Rabbit Polyclonal Isotype Control Antibody (Biolegend #CTL-4112). IP Buffer was added to lower the percentage of SDS < 0.1% ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies (all diluted at 1:100) were tested: PAX6 (rabbit, Biolegend Poly19013); FOSL1 aka Fra-1 (C-12 ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies and dilutions were used: anti-Keratin 6A (rabbit, 1:200, Biolegend_905701), anti-Ubiquityl-Histone H2A (Lys119 ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies and dilutions were used: anti-Keratin 6A (rabbit, 1:500, Biolegend_905701) 1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by the incubation with primary antibodies (anti-HA/NAMPT, rabbit polyclonal HA antibody, BioLegend Cat # ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 min) and resuspended in 100 μl of serum medium supplemented with 0.5 μl SSEA-I (BioLegend, cat. no. 125607) and 0.15 μl PE-CD61 antibodies (BioLegend ...
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bioRxiv - Neuroscience 2020Quote: ... Permeabilization was followed with blocking step by incubating the samples for 2 hr in PBS containing 3% normal goat serum (NGS, 927503, BioLegend) and 3% BSA and then stained overnight at 4°C with the primary monoclonal antibody (mouse anti-radixin ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspended cells were then transferred to a fresh flask and activated in fresh RPMI-1640/10% FBS/PSG by 3:20,000 anti-CD3 (BioLegend 317303), 3:20,000 anti-CD28 (BioLegend 302913) ...
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bioRxiv - Systems Biology 2021Quote: ... deeply anesthetized mice (5% isoflurane) were retro-orbitally injected with 3 μg of anti-CD45 antibody (PerCP, clone 30-F11, Biolegend) 3 min prior to euthanasia [46,81] ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 3 hours at 37 °C with complete IMDM medium with 1x Cell Activation Cocktail (with Brefeldin A, Biolegend). After stimulation ...
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bioRxiv - Cell Biology 2022Quote: ... BSA- and IgG-coated dynabeads for pre-clearing were prepared by mixing dynabeads slurry (40 μl per sample) with 500 μl PBS/3% BSA or 500 μl PBS with 2.5 μg mouse IgG1 (BioLegend, 400102) respectively ...
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Developmental Biology 2020Quote: Cells were resuspended in 5ml CMF-PBS with 3% BSA (MilliporeSigma, A9576-50ml) and plated on anti-GD3 (Biolegend, 917701) 10cm plates (untreated plates coated overnight at 4°C with 13μg GD3 antibody in 10ml 50mM Tris-HCl pH9.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Immunology 2019Quote: ... GolgiPlug™ and GolgiStop™ were added 3 h before cells were processed for surface and intracellular staining for CD4 (BV785-conjugated anti-human; OKT-4, BioLegend), CD8 (APC-H7-conjugated anti-human ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized with Ketamine/Xylazine (Patterson Veterinary) and injected intravenously with 3 μg of fluorophore-labelled anti-CD45 (30-F11, Biolegend) monoclonal antibody through the retro-orbital sinus to label intravascular leukocytes ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then washed with ice-cold FACS Buffer (1x PBS + 3% FBS) before staining with mouse anti-HA (diluted 1:200, Biolegend) for 30 minutes on ice ...