Insulin ELISA Kit 1 Plate citations for BioLegend

Quote: ... cells in black wall 96-well-plate were washed with PBS for once and blocked with FcR blocking buffer (FcX block, Biolegend, San Diego, CA, USA) at room temperature for 10 minutes ...

CD8⁺ T-Cell Metabolic Rewiring Defined by Single-Cell RNA-Sequencing Identifies a Critical Role of ASNS Expression Dynamics in T-Cell Differentiation

Juan Fernández-García, et al., bioRxiv - Immunology 2021

Quote: ... CD8+ T cells isolated at each time point of interest were directly stained in round-bottom 96-well plates using the following surface-marker antibodies (either from eBioscience, BioLegend, or produced in house): anti-mouse CD8a FITC (clone 53.6.7 ...

CD8⁺ T-Cell Metabolic Rewiring Defined by Single-Cell RNA-Sequencing Identifies a Critical Role of ASNS Expression Dynamics in T-Cell Differentiation

Juan Fernández-García, et al., bioRxiv - Immunology 2021

Quote: ... The resulting whole-splenocyte single-cell suspensions were directly stained in round-bottom 96-well plates using the following surface-marker antibodies (either from eBioscience, BioLegend, or produced in house): anti-mouse CD8a FITC (clone 53.6.7 ...

AMPK Drives Both Glycolytic and Oxidative Metabolism in T Cells During Graft-versus-host Disease

Archana Ramgopal, et al., bioRxiv - Immunology 2023

Quote: Levels of murine cytokines in transplanted mice were assessed using the LEGENDplex™ Murine Inflammation panel (13-plex) in a V-bottom plate per manufacturer’s instructions (Cat. No: 740446, BioLegend, San Diego, CA, USA). LEGENDPlex data were acquired on a BD Fortessa analyzer (BD Biosciences ...

Neutral sphingomyelinase 1 regulates cellular fitness at the level of ER stress and cell cycle

Dolma Choezom, Julia Christina Gross, bioRxiv - Cell Biology 2022

Quote: ... FITC Annexin V Apoptosis Detection Kit with PI (BioLegend) was used according to the manufacturer’s instructions ...

Metabolic and Epigenomic Regulation of Th17/Treg Balance by the Polyamine Pathway

Chao Wang, et al., bioRxiv - Immunology 2020

Quote: ... Apoptosis was assessed using Annexin V staining kit (BioLegend). Phosphorylation of proteins to determine cell signaling was performed with BD Phosflow buffer system (BD bioscience ...

Dynamically Linking Influenza Virus Infection Kinetics, Lung Injury, Inflammation, and Disease Severity

Margaret A. Myers, et al., bioRxiv - Systems Biology 2021

Quote: ... followed by viability (Biolegend, Zombie Violet Fixable Viability Kit) and surface marker staining with anti-mouse antibodies ...

Redefining CD4 T cell residency: Helper T cells orchestrate protective humoral immunity in the lung

Nivedya Swarnalekha, et al., bioRxiv - Immunology 2020

Quote: ... Live-dead stain (Zombie Red Fixable Viability kit, Biolegend) was added to the antibody mix and stained at 4°C for 30 min ...

Tyrosine kinase inhibitors trigger lysosomal damage-associated cell lysis to activate the NLRP3 inflammasome

Emilia Neuwirt, et al., bioRxiv - Immunology 2022

Quote: ... Annexin V-FITC apoptosis staining/detection kit (Abcam, BioLegend) and acridine orange (Invitrogen) ...

Clearance of defective muscle stem cells by senolytics reduces the expression of senescence-associated secretory phenotype and restores myogenesis in myotonic dystrophy type 1

Talita C. Conte, et al., bioRxiv - Cell Biology 2022

Quote: Apoptotic cells were identified using a detection kit (Biolegend, FITC Annexin V Apoptosis Detection Kit with PI ...

Resilient anatomy and local microplasticity of naïve and stress hematopoiesis

Qingqing Wu, et al., bioRxiv - Physiology 2022

Quote: ... LEGENDScreen™ Mouse PE Kit (BioLegend, catalogue number 700005) was used according to the manufacturer’s instructions ...

CD8⁺ T cell priming in oral mucosa-draining lymph nodes supports systemic immunity

Juliana Barreto de Albuquerque, et al., bioRxiv - Immunology 2020

Quote: ... or MojoSort™ Isolation Kits (BioLegend, San Diego, US) according to the manufacturer’s protocol ...

Endophilin A2 regulates B cell protein trafficking and humoral responses

Dessislava Malinova, et al., bioRxiv - Immunology 2020

Quote: ... CD138 (281-2) and c-kit (2B8) from BioLegend.

Machine learning predictions of MHC-II specificities reveal alternative binding mode of class II epitopes

Julien Racle, et al., bioRxiv - Immunology 2022

Quote: ... cells were incubated with Fix/perm kit (Biolegend 426803) for 20 min at 4°C in the dark ...

Bordetella spp. block eosinophil recruitment to suppress lung iBALT formation

Nicholas J. First, et al., bioRxiv - Microbiology 2022

Quote: ... MojoSort™ Mouse CD3 T Cell Isolation Kit (BioLegend). T cells were then seed in a 96 well plate containing 106 cells per well in a volume of 200 μl with RPMI (Gibco ...

In Vivo Biocompatibility of ZIF-8 for Slow Release via Intranasal Administration

Sneha Kumari, et al., bioRxiv - Pharmacology and Toxicology 2023

Quote: ... Deep blue cell viability kit was purchased from Biolegend. 0.3% carbon monoxide + 0.3% neon + 21% oxygen + balance nitrogen gas mix cylinder was purchased from SCI Analytical Laboratories ...

Age-associated B cell infiltration in salivary glands represents a hallmark of Sjögren’s-like disease in aging mice

Harini Bagavant, et al., bioRxiv - Immunology 2024

Quote: ... Zombie Aqua fixable viability kit (Biolegend, San Diego, CA) was used for live/dead cell discrimination ...

The ICF2 gene Zbtb24 specifically regulates the differentiation of B1 cells via promoting heme synthesis

Jun Wang, et al., bioRxiv - Immunology 2024

Quote: ... MojoSortTM Mouse Pan B Cell Isolation Kit II (Biolegend) were used to enrich CD19+ B cells in splenocytes before sorting.

Cytotoxic CD4⁺ T cells in the bone marrow compromise healthy ageing by enhancing granulopoiesis

Enrique Gabandé-Rodríguez, et al., bioRxiv - Immunology 2024

Quote: ... the Zombie Yellow™ Fixable Viability Kit (BioLegend, 423104) or the Ghost Dye™ Violet 540 (Tonbo Biosciences ...

Cooperative progression of colitis and leukemia modulated by clonal hematopoiesis via PTX3/IL-1β pro-inflammatory signaling

Hang He, et al., bioRxiv - Immunology 2023

Quote: ... and the fluorescence-conjugated antibodies: c-Kit (#105812, BioLegend), Sca-1 (#108126 ...

“Two-Birds-One-Stone” Oral Nanotherapeutic Designed to Target Intestinal Integrins and Regulate Redox Homeostasis for Ulcerative Colitis Treatment

Long Huang, et al., bioRxiv - Bioengineering 2024

Quote: ... The Annexin-V/PI kit was purchased from BioLegend, Inc ...

Mycobacterium tuberculosis-specific CD4 T cells expressing transcription factors associate with bacterial control in granulomas

Nicole L. Grant, et al., bioRxiv - Immunology 2022

Quote: ... True nuclear transcription factor buffer kit (BioLegend, Cat.#424401) was used for detection of transcription factors within cells as per the manufacturer’s recommendation ...

Adult thymus-derived cMaf⁺RORγt⁺ γδ T cells lack Scart2 chromatin accessibility and do not reach periphery

Tao Yang, et al., bioRxiv - Immunology 2023

Quote: ... Zombie Aqua and Zombie NIR Fixable Viability Kit (BioLegend) were employed for dead cell exclusion ...

Upregulated Fcrl5 disrupts B cell anergy and causes autoimmunity

Chisato Ono, et al., bioRxiv - Immunology 2023

Quote: ... or MojoSort Mouse Pan B cell Isolation Kit (BioLegend), following the manufacturer’s instructions ...

Microbiome ssRNA as an environmental cue to activate TLR13-dependent tissue-protective programs in CD5L^hi hepatic macrophages

Ryota Sato, et al., bioRxiv - Immunology 2023

Quote: ... The LEGENDScreen Mouse PE Kit was purchased from BioLegend.

Cellular complexity and crosstalk in murine TNF-dependent ileitis: Different fibroblast subsets propel spatially defined ileal inflammation through TNFR1 signalling

Lida Iliopoulou, et al., bioRxiv - Cell Biology 2023

Quote: ... or the Zombie-NIR Fixable Viability Kit (423105, Biolegend) was used for live-dead cell discrimination ...

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