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Citations for BioLegend :
1051 - 1100 of 1140 citations for 7 Amino 1H benzimidazole 5 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2020Quote: ... cells were pelleted for 5 minutes at 500xg and resuspended in 50 µL Intracellular Staining Perm Wash Buffer supplemented with 5 µL of TruStain FcX (Biolegend, 422302) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The frozen tissue sections were treated with blocking solution (5% normal donkey serum in PBS) and then stained with FITC-conjugated anti-Ly6G antibody (BioLegend, 1A8). Nuclei were stained by DAPI ...
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bioRxiv - Immunology 2020Quote: ... were resuspended in 100μl protein blocking solution with 5 μl fluorescent conjugated antibodies, CD8 (Biolegend Cat# 100714, CD45 (Biolegend Cat# 103130), CD68 (Biolegend Cat# 137004) ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were labeled with 5 µg ml−1 carboxylfluorescein diacetate succinimidyl ester (CFSE) or Tag-IT Violet™ proliferation and cell tracking dye (Biolegend) in PBS containing 5 mM MgSO4 ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... single-cell spleen suspensions from C57BL/6J mice were cultured at 5 million cells/ mL in complete RPMI with 1 μg/mL anti-CD3 (BioLegend, #100340) and 1 μg/mL anti-CD28 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated in primary antibodies diluted in 5% skim milk in TBST: mouse anti-brevican (1:1000, BioLegend, cat# 820101), sheep anti-neurocan (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Then the live microglial cells were incubated with PBS supplemented with 5% BSA + HLA-DR mouse monoclonal antibodies pre-conjugated with FITC (1:100, BioLegend, Inc.) + Hoechst 33342 (1:5000 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with their respective primary antibodies diluted in 5% skim milk in PBST at 4 °C overnight: purified mouse anti-βIII-tubulin (1:1,000) (BioLegend, cat. 801202), monoclonal mouse anti-GAPDH (1:50,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 1 x 106 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named ‘FACS buffer’) together with 5 uL of TruStain FcX (BioLegend, 422301). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Immunology 2024Quote: ... followed by 5 µL/well of the antibody cocktail for extracellular staining: anti-CD3-BV421 (clone 17A2) (BioLegend, SanDiego, CA, USA) (1:200) ...
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bioRxiv - Bioengineering 2024Quote: Resting T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Biophysics 2023Quote: ... The gel substrate for T cells had a Young’s modulus of 1.2 kPa and was coated with 5 μg/mL each of anti-CD3 (OKT3; 317326; BioLegend, CA, USA) and anti-CD28 (CD28.6 ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged and 500,000 cells were stained in 200 µl 1x PBS plus 0.5 µg of indicated antibody for 30 min in the dark: APC anti-mouse CD274 (B7-H1, PD-L1, Biolegend #124311) or appropriate IgG isotype ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then blocked in 5% NDS in TBS for 1 hour before being incubated with 1:1000 6E10 (BioLegend 803004) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Each well was blocked using 50 μL of FACS buffer (PBS + 5% FBS) + 2.5 μL Fc receptor (BioLegend, Cat No. 422302) block per well and then 150 μL FACS buffer was added and spun down ...
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bioRxiv - Molecular Biology 2023Quote: ... Collected cell populations were immediately seeded in tissue culture at a concentration of 2x105 cells per 1.9 cm2 in DMEM containing 5% FBS and 10 ng/mL TGFβ (Biolegend Cat 763102) or 500 nM Prostaglandin F (Cayman Chemical Cat 16010).
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bioRxiv - Neuroscience 2023Quote: ... The cell pellets were harvested and resuspended in 5 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 5 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) before run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) prior to run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (2,000 rpm 5 min) and then treated with 1 mL 1X red blood cell (RBC) Lysis Buffer (Biolegend; cat# NC9067514) to lyse red blood cells ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... washed cells were resuspended in 100 μL of FACS buffer with 5 μL of PE anti-human TMPRSS2 antibody (BioLegend #378403) per million cells and incubated for 30 min at 4 °C in the dark ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:500 in 1x Permeabilisation buffer with 5% Normal Donkey Serum at +40C and 1 h staining with DyLightTM 649 Donkey anti-rabbit IgG (BioLegend, #406406) diluted 1:500 in 1x Permeabilisation buffer ...
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bioRxiv - Immunology 2024Quote: The Fc receptors were blocked on 5X105 neutrophils resuspended in PBS with 5% FBS and TruStain FcX (1:50, BioLegend, 101320). Neutrophils were incubated with FITC conjugated anti-mouse Ly6G (1:50 ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... The cells were centrifuged at 300xg for 5 minutes at room temperature and resuspended in 85 ul of staining buffer and incubated with 5 ul of Human TruStain FcX(Biolegend cat: 422301) for 10 minutes on ice in 5 ml FACS tubes ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Following overnight incubation yeast were washed once in PBS/0.2% BSA and rotated at 18 rpm in same buffer containing 5 µg/mL mouse anti-His6 IgG (BioLegend, San Diego, CA) that had been conjugated with Alexa647 using the N-hydroxysuccinimidyl ester (Molecular Probes ...
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bioRxiv - Bioengineering 2021Quote: ... A 100 µL sample of EDTA-anticoagulated whole blood was incubated with 5 µL PE-Cy5 CD45 (HI30 clone, Biolegend, cat. # 304010) and 5 µL of PE-CD66B (Becton Dickinson ...
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bioRxiv - Biochemistry 2020Quote: ... Cells at a density of 2×107 cells/mL were incubated for 1 hour in flow cytometry buffer with 5 μL of Pacific Blue™ conjugated anti-CD235a antibody (BioLegend #349108) and 4 μg of unconjugated mouse IgG2a (Merck #M5409 ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 5×105 cells were stained for 30 min using the Zombie Aqua fixable viability dye (BioLegend Cat# 423101, 1:500 dilution), then fixed for 1 h in Foxp3/Transcription Factor Fixation/Permeabilisation Solution (ThermoFisher Cat# 00552300) ...
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bioRxiv - Immunology 2020Quote: ... Unbound antibodies were removed by washing with PBS were added at 5 × 104 cells/well in the presence of 4 μg/mL brefeldin A (Biolegend, Cat # 420601), 5 μg/mL GolgiStop (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Harvested lymphocytes were washed three times with FACS buffer (5% FBS in PBS) and stained for surface markers using: CD19-PE (363003, Biolegend, RRID: AB_2564125), IgD-FITC (348206 ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 75µL of Fc blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody, Biolegend in FACS buffer), and incubated for 20 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... plates were coated at least 5 hours prior to use with 3 ug/ml plate-bound anti-CD3 antibody (clone OKT3; BioLegend, LEAF grade), 1 ug/ml soluble anti-CD28 antibody (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... About 5×106 bone marrow cells were plated into 100mm non-tissue culture treated plates containing 20ng/ml GMCSF (Biolegend, Cat# 640920). On day 3 5ml of fresh media containing 20ng/ml of GMCSF was added to the cultures ...
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bioRxiv - Microbiology 2022Quote: ... the remaining volume was subjected to immunoprecipitation on protein A/G magnetic beads coated with 5 µg of mouse anti-HA IgG (Biolegend, clone 16B12) as described in the Magna RIP technical manual ...
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bioRxiv - Cell Biology 2024Quote: S6K phosphorylation was measured by intracellular staining in murine CD8+ T cells activated for 24 h with plate-bound anti-CD3 (coated at 5 µg / ml for 14 h at 4 °C in PBS; BioLegend, Cat. # 100331) and 1 µg / ml soluble anti-CD28 (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... Blocking occurred using 5% BSA in TBST and antibody probing using 2% BSA in TBST at the indicated dilutions: α-pERK1/2 (Biolegend 369502) 5000x ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells from CTLA4-deficient patients or healthy controls were purified from 5 million frozen PBMCs using a biotin labeled anti-human CD4 antibody (OKT4; BioLegend; 1:20) followed by magnetic separation with anti-biotin microbeads (Miltenyi Biotic ...