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Citations for BioLegend :
1051 - 1100 of 7955 citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... CD69-) from OT-I mice were stimulated with plate-bound anti-CD3 mAb (5µg/ml, clone 145-2C11, Biolegend), and soluble anti-CD28 (1µg/ml ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Immunology 2022Quote: ... Cell cultures were stimulated for 3 days with either 100 ng/mL soluble or 1.25 μg/mL immobilised (plate-bound) anti-CD3 mAb (clone OKT3, BioLegend) in combination with 1 μg/mL soluble anti-CD28 mAb (clone CD28.2 ...
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bioRxiv - Immunology 2022Quote: Plates (48- or 96-well, flat bottom) were coated with 10 μg/ml αCD3ε mAb (BioLegend, San Diego, CA) and incubated at 37°C for 5 hours ...
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bioRxiv - Immunology 2023Quote: ... 3x105 sorted naïve CD4 T cells were stimulated with plate-bound anti-CD3 (145-2C11, Cytek) (5 μg/ml) and soluble anti-CD28 (clone 37.51, Biolegend) (1 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... were added to the isolated lymphocyte suspension before seeding at 1mL/well in a 24-well plates coated with 1µg/mL of anti-CD3ε (100340, Biolegend) 24 h before the procedure.
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bioRxiv - Immunology 2024Quote: ... cells were removed by washing and the plates were washed and probed with biotin α-IFNγ (clone XMG1.2, Biolegend) followed by incubation with Streptavidin-ALP (Mabtech) ...
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bioRxiv - Genomics 2024Quote: ... were plated in a 96 well V-bottom plate (200,000 cells/well) and 1ug/mL peptides added to restimulate cells along with Monensin and Brefeldin A (Biolegend). Iberdomide (10nM ...
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bioRxiv - Immunology 2024Quote: ... 8×105 naïve CD8+ or CD4+ T cells have been stimulated with 10µg/ml anti-CD3 (plate-coated) (Biolegend 300465) and 2µg/ml anti-CD28 (Biolegend 302943 ...
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bioRxiv - Biochemistry 2024Quote: ... the BMMs were seeded into 6-well plates and incubated with macrophage colony-stimulating factor (M-CSF) (20_ng/ml, Biolegend) and Receptor activator of nuclear factor kappa-Β ligand (RANKL ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were seeded in 96-well plates and cultured alone or stimulated with anti-CD3 antibody (Clone: OKT3, Biolegend) or treated with 50 ng/ml IL-15 for 4 days ...
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bioRxiv - Immunology 2023Quote: ... 130-120-662) for 3 days in 24-well plates coated with 3μg/ml anti-CD3 (145-2c11; Biolegend) and 3μg/ml anti-CD28 (37.51 ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin-Streptomycin and IL-2 (100 IU/ml) and activated for 24 h in the presence of plate-bound anti-CD3 antibody (2.5 µg/mL; BioLegend) and soluble anti-CD28 antibody (2.5 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... A 96 well plate was pre-incubated with a 5 µg/ml solution of anti-mouse CD3e (100314, Biolegend) in sterile PBS at 37°C for 2 h ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2023Quote: ... Activation consisted of 5μg/mL plate-bound anti-mouse CD3ε antibody (100340) and 5μg/mL soluble anti-mouse CD28 antibody (102116) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend) and Sca-1 (Pacific Blue ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend) and Sca-1 (Pacific Blue ...
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bioRxiv - Cancer Biology 2022Quote: ... CD106 (1:100, MVCAM.A, #105717), CD62L (1:200, MEL-14, #104435), PD-1 (1:100, 29F.1A12, #135215) (Biolegend); Streptavidin (1:200 ...
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bioRxiv - Immunology 2023Quote: ... 1:300), NK1.1 (108736, 1:150), Tbet (644810, 1:50), TCRβ (109243, 1:100), TER119 (116228, 1:200) from Biolegend, CD19 (45-0193-82 ...
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bioRxiv - Immunology 2023Quote: ... 1:100), GR1 (108428, 1:300), NK1.1 (108728, 1:200), TCRβ (109243, 1:100), TER119 (116228, 1:200) from Biolegend, IL-22 (12-7221-82 ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 900 x g for 5 minutes at room temperature and stained with Zombie Aqua Fixable viability kit (Biolegend, 1:1000 diluted in PBS, 100µL) for 20 minutes at room temperature in the dark to enable exclusion of dead cells ...
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bioRxiv - Immunology 2022Quote: ... and transcription factor staining kit (Biolegend). To assess the effect of RORγt inhibition on IL-17 production the specific inhibitor SR1001 (Tocris ...
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bioRxiv - Immunology 2019Quote: ... CD117 (c-Kit) APC (Biolegend 105812), CD150 (SLAM ...
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bioRxiv - Cell Biology 2021Quote: ... c-Kit APC/Cy7 (Biolegend, 105826), Flk2 Biotin (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... c-Kit (Biolegend, Cat no. 105813), CD31 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... and c-Kit-APC/Cy7 (Biolegend), 50μg/mL Rat IgG (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Zombie UV Fixable Viability Kit (Biolegend) was used to exclude dead cells ...
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bioRxiv - Cell Biology 2022Quote: ... c-Kit-APC/Cy7 (Biolegend 105826), Sca1-BV421 (Biolegend 108133 ...
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bioRxiv - Immunology 2023Quote: ... c-Kit PerCP/Cy5.5 (BioLegend 135133), Il7ra PE/Cy7 (BioLegend 158209) ...
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bioRxiv - Immunology 2024Quote: ... and APC-c-kit (BioLegend, #135108), or an antibody cocktail for immune cells containing FITC-Ly6G (eBioscience ...
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bioRxiv - Cancer Biology 2024Quote: ... Zombie Aqua Fixable Viability Kit (BioLegend), and the FOXP3/Transcription Factor Staining Kit (eBioscience ...
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bioRxiv - Immunology 2023Quote: ... Zombie UV Fixable Viability Kit (Biolegend) was used to exclude dead cells ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-2 and TNFα kits (BioLegend) and the experiments were done according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Annexin V Apoptosis Detection Kit (Biolegend) was used ...
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bioRxiv - Immunology 2023Quote: ... Zombie NIR Fixable Viability Kit (Biolegend). Samples were blocked with anti-CD16/32 (2.4G2 ...
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bioRxiv - Microbiology 2023Quote: ... Zombie Aqua Fixable Viability Kit (Biolegend) was used to exclude dead cells ...
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bioRxiv - Cancer Biology 2023Quote: ... or AquaTM Fixable Viability Kit (BioLegend). Intracellular staining of immune cells was performed using the following Abs and a Foxp3 staining buffer kit (eBioscience ...
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bioRxiv - Cell Biology 2023Quote: ... Zombie NIR Fixable Viability Kit (BioLegend) staining was performed to exclude dead cells and debris from the analysis ...
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bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
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bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were washed with HBSS twice by centrifugation at 250 x g for 8 min and then suspended in BMDC culture medium (RPMI-1640 medium [Thermo Fisher Scientific] containing 10% FBS, 20 ng/ml recombinant mouse GM-CSF [BioLegend] ...
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bioRxiv - Cell Biology 2021Quote: ... PMNs and platelets were visualized by applying 20 μl of platelet-specific FITC-labeled X488 antibody (Emfret, Eibelstadt, Germany) and 20 µl of PE-labeled anti-Ly6G (BioLegend #127608) with a catheter ...
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bioRxiv - Immunology 2021Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...
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bioRxiv - Molecular Biology 2022Quote: ... an equal number of cells from each group were incubated at room temperature for 20 minutes with Zombie Aqua™ Fixable Viability dye (BioLegend) and blocked on ice for 20 minutes with FcBlock anti-CD16/CD32 (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: Primary mouse and human cells were isolated as described above and stained with Zombie fixable viability dye for 20 min at room temperature (#423102, BioLegend, USA) and pre-incubated with anti-mouse Fc block at 10 μg mL-1 (#101320 ...
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bioRxiv - Genetics 2024Quote: ... Another 400 μL of supernatant was mixed with 600 μL of FSPP buffer and 20 μL of pre-blocked Magnetic IgG beads and TAP antibody (Thermo CAB1001) or Rpb3 antibody (Biolegend 920204) for 10–12 hrs at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... a fixation/permeabilization step was performed by incubating the cells for 20 min at RT using a Cyto-Fast Fix/Perm Buffer set (#426803, BioLegend, USA). Intracellular antibody staining was then performed for 25 min at RT ...
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bioRxiv - Bioengineering 2023Quote: Streptavidin-coated nanovials were reconstituted at a five time dilution in Washing Buffer containing 140 nM (20 μg/mL) of each biotinylated antibody or cocktail of antibodies: anti-CD45 (Biolegend, 368534) and anti-IFN-γ (R&D Systems ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stainied with the follow antibodies for 20 min on ice: anti-CD45 (APC-Fire 750, 30-F11, BioLegend 103153), anti-CD3 (BV785 ...