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• A subset of CD4+ effector memory T cells limit immunity to pulmonary viral infection and prevent tissue pathology via activation of latent TGFβ

  CP McEntee, et al., bioRxiv - Immunology 2023

  Quote: ... Antibodies used for flow cytometry and FACS were purchased from Biolegend and/or eBioscience™ and are listed in Supplementary Table 3.
• A subset of CD4+ effector memory T cells limit immunity to pulmonary viral infection and prevent tissue pathology via activation of latent TGFβ

  CP McEntee, et al., bioRxiv - Immunology 2023

  Quote: ... washed in PBS and stained with fixable viability Zombie dyes (Biolegend) diluted in PBS ...
• Microbiota epigenetically direct tuft cell differentiation to control type 2 immunity

  Emily M. Eshleman, et al., bioRxiv - Immunology 2023

  Quote: ... PE-CF594-anti-CD25 (Clone: PC61, BioLegend), PE-anti-SiglecF (Clone ...
• 3D Confinement-enabled Priming of Synaptic Activation Promotes Primary T Cell Expansion

  Ruoyu Jiang, et al., bioRxiv - Immunology 2023

  Quote: ... 100 μL of cell suspension was mixed with Pacific Blue anti-human Ki-67 (Biolegend), followed by incubation at room temperature in the dark for 30 min ...
• 3D Confinement-enabled Priming of Synaptic Activation Promotes Primary T Cell Expansion

  Ruoyu Jiang, et al., bioRxiv - Immunology 2023

  Quote: Step-by-step protocol was followed for flow cytometry analysis for cell suspension samples (Biolegend, San Diego, CA). For T cell activation experiments ...
• CD4⁺ T cells display a spectrum of recall dynamics during re-infection with malaria parasites

  Hyun Jae Lee, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were then stained with Zombie Yellow viability dye (Biolegend) or Live/Dead aqua (Invitrogen) ...
• Primary lung fibroblasts respond to IL-33, IL-13, and IL-17A by secreting factors that activate macrophages

  Jarrett Venezia, et al., bioRxiv - Immunology 2023

  Quote: ... or anti-mouse CD11b-BV785 (clone M1/70, BioLegend). Single-fluorophore controls were prepared using either cytometry beads (Becton Dickinson 552843 ...
• Primary lung fibroblasts respond to IL-33, IL-13, and IL-17A by secreting factors that activate macrophages

  Jarrett Venezia, et al., bioRxiv - Immunology 2023

  Quote: Cultured lung fibroblasts were lifted by incubating cells with Accutase (BioLegend 423201) for up to 20 minutes at room temperature ...
• Primary lung fibroblasts respond to IL-33, IL-13, and IL-17A by secreting factors that activate macrophages

  Jarrett Venezia, et al., bioRxiv - Immunology 2023

  Quote: Recombinant (r) mouse cytokines were purchased from BioLegend: rIL-33 (580502) ...
• Integration of Avidity and Differentiation is enabled by CD8⁺ T-cell sensing of IFN-γ

  Lion F.K. Uhl, et al., bioRxiv - Immunology 2023

  Quote: Surface markers used for flow cytometry analysis included: anti-CD8 (Biolegend, clone: 53-6.7), anti-CD4 (Biolegend ...
• Integration of Avidity and Differentiation is enabled by CD8⁺ T-cell sensing of IFN-γ

  Lion F.K. Uhl, et al., bioRxiv - Immunology 2023

  Quote: ... Antibodies used for intracellular cytokine staining included anti-IFN-gamma (Biolegend, clone: XMG1.2) and anti-TNF (Biolegend ...
• Integration of Avidity and Differentiation is enabled by CD8⁺ T-cell sensing of IFN-γ

  Lion F.K. Uhl, et al., bioRxiv - Immunology 2023

  Quote: ... TruStain FcX™ (anti-mouse CD16/32, Biolegend, #101319) and fluorochrome-conjugated primary antibodies (Biolegend ...
• Unfolded Protein Response Factor ATF6 Augments T Helper Cell Responses and Promotes Mixed Granulocytic Airway Inflammation

  Dandan Wu, et al., bioRxiv - Immunology 2023

  Quote: ... IL-4 (11B11) and IL-5 (TRFK5) were from Biolegend. Following surface and/or intracellular stain ...
• Signaling Node at TSC2 S1365 Potently Regulates T-Cell Differentiation and Improves Adoptive Cellular Cancer Therapy

  Chirag H Patel, et al., bioRxiv - Immunology 2023

  Quote: ... Antigens were specifically stained using the following antibody clones for flow cytometry where indicated: CCR7 (CD197, Biolegend); CD45RA (H1100 ...
• Subset-specific and temporal control of effector and memory CD4+ T cell survival

  Sharmila Shanmuganad, et al., bioRxiv - Immunology 2023

  Quote: ... T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend, clone 145-2C11) and 1 μg/mL anti-CD28 (BioLegend ...
• Subset-specific and temporal control of effector and memory CD4+ T cell survival

  Sharmila Shanmuganad, et al., bioRxiv - Immunology 2023

  Quote: ... IL-2 (Biolegend), CD127 ...
• Liver-specific Inflammatory Signatures Predict Clinically Significant Liver Damage

  Conan Chua, et al., bioRxiv - Immunology 2023

  Quote: ... Results were acquired on a LSR Fortessa (Becton Dickinson) cytometer and analyzed using LEGENDplex™ Data Analysis Software (BioLegend).
• The landscape of MHC-presented phosphopeptides yields actionable shared tumor antigens for cancer immunotherapy across multiple HLA alleles

  Zaki Molvi, et al., bioRxiv - Immunology 2023

  Quote: ... and HLA-A3 PE-Vio770 (Miltenyi, 1:100) or HLA-C AF647 (BioLegend, 1:100). Stained cells were washed twice and acquired on a BD LSRII.
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... anti-GM-CSF (502305) all from BioLegend and anti-CCL20 (C360A ...
• Single cell transcriptomic analysis of renal allograft rejection reveals novel insights into intragraft TCR clonality

  Tiffany Shi, et al., bioRxiv - Immunology 2023

  Quote: ... Co-culture supernatant was collected following completion of culture and analyzed for IL-2 using the ELISA MAX™ Deluxe Set for human IL-2 (Biolegend, 431804) at a dilution of 1:5.
• Single cell transcriptomic analysis of renal allograft rejection reveals novel insights into intragraft TCR clonality

  Tiffany Shi, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were analyzed and sorted for transfection efficiency via flow cytometry analysis using anti-human CD3 APC/Cy7-conjugated antibody and anti-human CD34 PE-conjugated antibody for TCR-expressing cells (BioLegend, 300426 at 10 μg/mL and BioLegend, 343606 at 1.25 µg/mL).
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... cells were incubated with Alexa Fluor 594 streptavidin (1:500, 405240; BioLegend) for an additional 30 min ...
• Single cell transcriptomic analysis of renal allograft rejection reveals novel insights into intragraft TCR clonality

  Tiffany Shi, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were analyzed and sorted for transfection efficiency via flow cytometry analysis using anti-human CD3 APC/Cy7-conjugated antibody and anti-human CD34 PE-conjugated antibody for TCR-expressing cells (BioLegend, 300426 at 10 μg/mL and BioLegend ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... all from BioLegend and anti-human CCR6 PECy7 (560620 ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were incubated with anti-human CCR2-biotin (FAB151B; R&D Systems/ 357224; BioLegend) in FACS buffer (HBSS ...
• The role of B cells in immune cell activation in polycystic ovary syndrome

  Angelo Ascani, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse IgG1 (Biolegend; RMG1-1; at 2 μg/ml), antimouse IgG2b (BD Biosciences ...
• Defective callosal termination underlies the long-term behavioral deficits in NMDA receptor antibody encephalitis mouse model

  Jing Zhou, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD38 PerCPCy5.5 (BioLegend HIT2), CD3 PE-Cy7 (Beckman UCHT1) ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: ... CD86-BV605 (Biolegend, Cat# 105037), Ly6G-SB702 (Invitrogen ...
• A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPS

  Yining Liu, et al., bioRxiv - Bioengineering 2023

  Quote: ... These cells were then stained with Zombie NIR (BioLegend) for 15 minutes at room temperature to access the viability and blocked with Fcr Blocking Reagent (Miltenyi Biotec ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: ... CD64-PE (Biolegend, Cat# 139304), F4/80 PE/Dazzle (Biolegend ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: ... CD24-FITC (Biolegend, Cat# 101806), CD44-PerCP-Cy5.5 (Tonbo Biosciences ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: ... B220-PE-Cy5 (Biolegend, Cat# 103210), CD19-PE-Cy7 (Invitrogen ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: ... F4/80 PE/Dazzle (Biolegend, Cat# 123146), B220-PE-Cy5 (Biolegend ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: ... CD11c-AF647 (Biolegend, Cat# 117312), MHCII-RedFluor710 (Tonbo Biosciences ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: ... CD103-APC (Biolegend, Cat# 121414), CD11c-AF647 (Biolegend ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: ... CD11b-BV570 (Biolegend, Cat# 101233), CD103-APC (Biolegend ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: ... Ly6C-BV785 (Biolegend, Cat# 128041), CD64-PE (Biolegend ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: ... and spleens were stained with the following antibodies: CD45-BV421 (Biolegend, Cat# 10314), CD40-Pacific Blue (Biolegend ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: ... Nunc MaxiSorp ELISA plates (Biolegend, Cat# 423501) were coated with 10μg/ml trimeric spike protein overnight at 4°C ...
• IGF2BP1/IMP1 deletion enhances a facultative stem cell state via regulation of MAP1LC3B

  Louis R. Parham, et al., bioRxiv - Cell Biology 2023

  Quote: ... cells were sorted for highest 5% GFP expression using Zombie Aqua (BioLegend 423101) and the MoFlo Astrios (Beckman Coulter).
• IGF2BP1/IMP1 deletion enhances a facultative stem cell state via regulation of MAP1LC3B

  Louis R. Parham, et al., bioRxiv - Cell Biology 2023

  Quote: ... CD24-APC (1:500, BioLegend Cat# 101813); UEA-1 (1:500 ...
• Cooperative sensing of mitochondrial DNA by ZBP1 and cGAS promotes cardiotoxicity

  Yuanjiu Lei, et al., bioRxiv - Immunology 2023

  Quote: ... and Anti-DYKDDDDK Tag (L5) Affinity Gel Antibody (Biolegend, 651502) were used for pre-cleaning and IP ...
• Diet Induced Obesity and Diabetes Enhance Mortality and Reduces Vaccine Efficacy for SARS-CoV-2

  Robert M Johnson, et al., bioRxiv - Microbiology 2023

  Quote: ... CD8a APC (BioLegend clone 53-6.7), CD19 PE (BioLegend clone 6D5) ...
• Diet Induced Obesity and Diabetes Enhance Mortality and Reduces Vaccine Efficacy for SARS-CoV-2

  Robert M Johnson, et al., bioRxiv - Microbiology 2023

  Quote: ... CD11b Brilliant Violet 650 (BioLegend, clone M1/70), Ly6G Brilliant Violet 785 (BioLegend ...
• Diet Induced Obesity and Diabetes Enhance Mortality and Reduces Vaccine Efficacy for SARS-CoV-2

  Robert M Johnson, et al., bioRxiv - Microbiology 2023

  Quote: ... CD65 Brilliant Violet 605 (BioLegend, clone X54-5/7.1), CD103 BUV 661 (BD ...
• Diet Induced Obesity and Diabetes Enhance Mortality and Reduces Vaccine Efficacy for SARS-CoV-2

  Robert M Johnson, et al., bioRxiv - Microbiology 2023

  Quote: ... Brefeldin A and Monensin (BioLegend). The peptide pool consisted of overlapping 15-mers by 10 spanning entire protein ...
• Diet Induced Obesity and Diabetes Enhance Mortality and Reduces Vaccine Efficacy for SARS-CoV-2

  Robert M Johnson, et al., bioRxiv - Microbiology 2023

  Quote: ... and IL-17a BV711 (BioLegend, clone: TC11-18H10.1). Fixed cells were washed once in PBS containing 3% FBS and resuspended in PBS containing 3% FBS ...
• Diet Induced Obesity and Diabetes Enhance Mortality and Reduces Vaccine Efficacy for SARS-CoV-2

  Robert M Johnson, et al., bioRxiv - Microbiology 2023

  Quote: ... The antibodies used were as follows: IFN-γ BV421 (BioLegend, clone: XMG1.2), TNF-α BV650 (BioLegend ...
• Diet Induced Obesity and Diabetes Enhance Mortality and Reduces Vaccine Efficacy for SARS-CoV-2

  Robert M Johnson, et al., bioRxiv - Microbiology 2023

  Quote: ... TNF-α BV650 (BioLegend, clone:MP6-XT22), IL-4 BV605 (BioLegend ...
• Diet Induced Obesity and Diabetes Enhance Mortality and Reduces Vaccine Efficacy for SARS-CoV-2

  Robert M Johnson, et al., bioRxiv - Microbiology 2023

  Quote: ... and NK1.1 Pacific Blue (BioLegend, clone PK136), CD65 Brilliant Violet 605 (BioLegend ...