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Citations for BioLegend :
851 - 900 of 3466 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 20 µl/ml mouse serum and 10 µl/ml Fc block (CD16/32) (Biolegend, CA). Plates were incubated for 2 hrs at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were subsequently blocked with 2 μL of TruStain FcX Fc receptor blocking antibody (BioLegend) for 10 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... cells were washed once with 1x PBS and incubated in TruStain FcX-Fc Block (Biolegend) for 15 min at 4°C in the dark ...
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bioRxiv - Immunology 2019Quote: ... Singlecell suspensions from spinal cord were incubated with Fc block (CD16/32-clone 93, BioLegend) and then stained with fluorescent-conjugated antibodies for surface markers ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were first blocked for 5 minutes using CD16/CD32 (Biolegend, San Diego, CA). Eosinophils were identified as CD11c-Siglec-F+ and neutrophils were identified as SiglecF-GR-1+ ...
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bioRxiv - Immunology 2022Quote: Single-cell suspensions were incubated in Fc Block (TruStain fcX anti-mouse CD16/32, Biolegend) for 15 min on ice and washed with PBS ...
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bioRxiv - Immunology 2022Quote: ... to distinguish dead cells and then treated with Fc Block (BioLegend, San Diego, CA, USA) to block nonspecific binding ...
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bioRxiv - Immunology 2022Quote: ... cells were washed and blocked using Trustain Fc receptor blocking solution (Biolegend cat. no. 422302) for 10min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were FC-blocked with anti-CD16/32 antibody (BioLegend Cat# 101320, RRID:AB_1574975; 1:100) for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... and then blocked with anti-mouse CD16/CD32 Fc block (Biolegend, San Diego, CA, USA) and unlabeled MR1-6-formylpterin-tetramer (6-FP ...
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bioRxiv - Bioengineering 2023Quote: ... 1.5×106 cells were stained in PBS with 1:200 CD16/CD32 Fc Block (Biolegend) and 1:500 Live/Dead fixable dye (ThermoFisher ...
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bioRxiv - Bioengineering 2024Quote: ... Each tube of cells then received 2 µL of Fc block (BioLegend, San Diego, CA), 10 µL of Brilliant Stain Buffer (Becton Dickinson) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were pre-incubated with 1 μg/mL of αCD16/32 Fc-Block (BioLegend 101310) in 30 μl staining buffer (PBS ...
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bioRxiv - Immunology 2023Quote: ... J3 and A6-edited cells were stained with anti-IgG-Fc antibody (BioLegend, clone M1310G05). Cells were sorted on a FACSAria II cell sorter based on membrane IgG expression (J3 and A6 editing ...
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bioRxiv - Cancer Biology 2023Quote: ... All stainings were performed in presence of Fc Block/anti-mouse CD16/32 (93; Biolegend).
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bioRxiv - Neuroscience 2023Quote: ... containing ssDNA (1 mg/mL final concentration) and FC block (1:100 of BioLegend, 156604), was added ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend). PBMC were washed in cold PBS and seeded at 1 × 106 cells/well in 96-well round bottom plates and with combinations of RBD and decoy tetramers by incubation for 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend).
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bioRxiv - Microbiology 2020Quote: ... IL-18 and IFN-γ from human PBMCs were measured by LEGENDplex™ Human Inflammation Panel (Biolegend) according to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Human CD14+ cells were isolated from healthy donor PBMCs using MojoSort Human CD14 Selection Kit (Biolegend #480026), and were cultured with 50 ng/ml recombinant human GM-CSF (BioLegend #572904 ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained with anti-human CD45 (PE) and anti-human CD117 (APC) (Biolegend, San Diego, CA, USA). CD45-low/CD117+ leukemia cells were FACS sorted (MA900 sorter ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cells were sorted after staining with anti-human specific antibodies directed against EpCAM and CD298 (Biolegend) with simultaneous depletion of mouse cells stained with anti-mouse-specific antibodies directed against CD45 and CD31 (Biolegend).
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bioRxiv - Immunology 2020Quote: ... anti-human BDCA-2-PE (clone AC144; Miltenyi Biotech) and anti-human CD123-APC (clone 6H6; Biolegend). Cells were incubated with surface antibodies at 4°C for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend) at a 1:2000 dilution in TBST with 3% BSA and 0.02% sodium azide overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: ... and PE anti-DYKDDDDK Tag antibody (0.6 ul antibody per million cells in 100 ul) (Biolegend #637310) for DYKDDDDK-tagged Cas9 was carried out at room temperature for 45 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... Expression was validated by flow cytometry using Alexa Fluor 647-conjugated anti-DYKDDDDK tag antibody (BioLegend #637315).
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bioRxiv - Microbiology 2021Quote: Mouse anti-HA.11 (Clone 16B12) and Mouse anti-HIS tag (Clone J099B12) were purchased from BioLegend. Rat anti-HA (Clone 3F10 ...
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bioRxiv - Cell Biology 2023Quote: ... Tau-tau sensor molecules interaction was assessed using two monoclonal antibodies against the HA tag (mouse, Biolegend cat ...
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bioRxiv - Synthetic Biology 2023Quote: ... stable pools of cells were analyzed by flow cytometry using APC anti-Flag-tag antibody (Biolegend; 637307). Stable pools went through another round of selection in complete media supplemented with with 0.7ug/mL puromycin and 1ng/mL human prolactin or purified mouse prolactin and were analyzed by flow cytometry ...
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bioRxiv - Bioengineering 2023Quote: The antibodies used for flow cytometry were as follows: anti-His Tag APC (BioLegend, USA, Cat: #362605), anti-human IgG (G18-145 ...
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bioRxiv - Immunology 2022Quote: ... Bound virus was detected with rabbit polyclonal VP1 antibody (1:10000) followed by an anti-rabbit secondary (Biolegend, 1:200). Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... a multiplex cytokine quantification (LEGENDplex HU Anti-Virus Response Panel (13-plex) and LEGENDplex HU Th Cytokine Panel (13-plex) (cat# 740390 and cat# 740722, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Cytokines and chemokines present in the sera of uninfected vs MCMV-infected mice and in the BAL of uninfected vs IAV-infected mice were analyzed using the LEGENDplex Mouse Anti- Virus Response Panel (BioLegend). Samples and standards were plated in technical duplicates and the assay was executed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in FACS buffer with FC block (anti-CD16/CD32, 1:500, Biolegend 101330) and incubated for 5 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... all cell suspensions were Fc blocked with rat anti-mouse CD16/32 (clone 93, BioLegend, USA) and then stained with 10 μg/mL AlexaFluor647 Armenian hamster anti-mouse CD3ε (clone 145-2C11 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fc receptors were blocked using TruStain FcX PLUS (anti-mouse CD16/32, 1:200 dilution) (BioLegend). In pilot experiments to determine localization of NC in leukocytes (CD45+ ...
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bioRxiv - Developmental Biology 2022Quote: ... Fc-Receptor block treatment was performed using an anti-mouse CD16/32 antibody (BioLegend, Clone: 93) or anti-human Fc receptor block (biolegend ...
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bioRxiv - Immunology 2020Quote: ... Cells were pre-incubated with TruStain Fc Receptor Block (anti-mouse CD16/CD32, clone 93, BioLegend), then labeled with extracellular antibodies including ...
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Two subsets of human marginal zone B cells resolved by global analysis of lymphoid tissues and bloodbioRxiv - Immunology 2021Quote: ... Cells were then incubated for 10 minutes at 4°C with True stain Fc block (BioLegend) and washed ...
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bioRxiv - Genomics 2020Quote: ... cells were then incubated for 10 min with Fc receptor block (PN 422301, TruStain FcX, BioLegend) to block nonspecific antibody binding ...
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bioRxiv - Immunology 2020Quote: ... Single-cell suspensions were treated with CD16/32 Fc blocking antibodies (Biolegend; catalog 101310; clone 93) to prevent non-specific antibody binding and stained with the fluorescent-labeled antibodies for 20 minutes followed by fixation using 1% paraformaldehyde ...
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bioRxiv - Cancer Biology 2022Quote: ... and pre-incubated with anti-mouse Fc block at 10 μg mL-1 (#101320, BioLegend, USA) or anti-human Fc block (#422302 ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were treated with CD16/32 Fc blocking antibodies (Biolegend; catalog 101310; clone 93) to prevent non-specific antibody binding and stained with the fluorescent-labeled antibodies for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... and bone marrow were incubated with Fc-blocking TruStain FcX™ (anti-mouse CD16/32, BioLegend) antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... one million cells were first resuspended in100LμL buffer containing 5LμL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15Lminutes ...
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bioRxiv - Immunology 2024Quote: ... then anti CD16 /32 were added for Fc- receptors blocking on ice for 10 minutes (Biolegend, Purified anti-mouse CD16/32 AntibodyRat IgG2a ...
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bioRxiv - Immunology 2024Quote: DAPI (#11034-56, Nacalai Tescue) and Fc block (clone 93, BioLegend or clone 2.4G2, Tonbo Biosciences) were added to the cells just before staining with specific antibodies against cell surface molecules ...
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bioRxiv - Cell Biology 2023Quote: ... followed by Fc receptor blockade for 10 min and then incubation in permeabilization buffer (BioLegend, 421002) for 15 min ...