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Citations for BioLegend :
851 - 900 of 950 citations for 6 Methylchroman 4 ylamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... clones that survived after 3-4 months of culture were washed with PBS and stained with FITC anti-HLA-DQ (Biolegend, Clone: HLADQ1) for 30 minutes on ice and cell surface expression assessed by flow cytometry ...
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bioRxiv - Systems Biology 2021Quote: Cells were stained overnight at 4°C in blocking solution with the following antibodies: AlexaFluor® 488 anti-Vimentin (1:1000, Biolegend, #677809), AlexaFluor® E-cadherin (1:200 ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... samples P008-016 and P048-119 (Suppl. Fig. 4D) were labelled with TotalSeq B Hashtag antibodies 1-4 (Biolegend, clone LNH-94) during the staining procedure described above and pooled in groups of four.
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bioRxiv - Bioengineering 2022Quote: ... For immune cells detections the tumoroids sections also were incubated overnight at 4°C with anti-B220 (Biolegend 103224, APC-cy7 conjugated) and anti-cd11b (Biolegend 101208 ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... supernatant was aspirated and the cells were incubated in blocking buffer mixture for 5min at 4°C (1uL anti-mouse CD16/32 TruStain Fcx (RRID: AB-1877271 AB-1574973, Biolegend Cat #: 101320) and 99uL FACS buffer (1% BSA + 0.05% sodium azide in HBSS w/o Ca2+/Mg2+)) ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were collected by centrifugation (1500rpm; 5mins; 4°C) and washed twice with Annexin V binding buffer (Biolegend; 1ml per wash). The cell pellets were resuspended in of binding buffer (100μl ...
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bioRxiv - Immunology 2020Quote: ... Unbound antibodies were removed by washing with PBS were added at 5 × 104 cells/well in the presence of 4 μg/mL brefeldin A (Biolegend, Cat # 420601), 5 μg/mL GolgiStop (BD Biosciences ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then incubated overnight at 4°C with primary antibody diluted in blocking solution (rabbit anti-FLAG, Sigma, 1:250 or mouse anti-Tuj1, Biolegend 1:500). After washing in PBS and incubation with secondary antibody (Alexa 647 anti-goat IgG 1:500 ...
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bioRxiv - Immunology 2020Quote: ... Then these PBMCs was incubated with mixed antibodies cocktail at 4 °C for 30 min (the antibodies cocktail including FITC-anti-human CD19 antibody (Biolegend, clone: SJ25C1), BV421-anti-human IgD antibody (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... cytokine staining mixture (containing anti-IFN-γ, anti-IL-2, anti-IL-4, anti-IL-10 and anti-TNF-α antibodies, Biolegend) was added to each well ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and stained in PermWash buffer (eBioscience) for 30 min at 4°C with Abs (purchased from BioLegend unless noted) against Bcl2 (BCL/10C4) ...
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bioRxiv - Immunology 2021Quote: ... Cell surface proteins were stained for 20 min at 4 °C with the following antibodies: CD45.1 (BioLegend, 110714, Clone ID: A20, RRID:AB_313503), CD45.2 (BioLegend ...
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bioRxiv - Immunology 2021Quote: 4*106 peripheral blood mononuclear cells (PBMCs) per patient were first incubated with Human TruStain FcX™ blocking reagent (Biolegend, Cat# 422302) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Staining for surface markers of T cells was carried out in the dark at 4°C for 15 – 30 minutes using the following antibody panel: BV510-CD45 (clone 30F11; Biolegend, CA, USA), PE-Cy7-TCRβ (clone H57-597 ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend, cat. 422101) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... then stained for 30 minutes at 2-8°C with the following cytokine antibodies: Granzyme B (GzB) BV421 (4 μg/mL, BioLegend Cat. 396414), IFN-gamma (IFN-γ ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.3% PBST for 3 days at 4°C on rotating incubator (NFH, 1:500, Abcam, ab4680; Tubulin β-III, 1:200, Biolegend, 801201). Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568 ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with 1x Perm buffer (Tonbo) and stained for intracellular antigens for 30 mins at 4°C with the following antibodies: GranzymeB: AF647 (Biolegend, Clone: GB11), IL-2 ...
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bioRxiv - Cell Biology 2024Quote: S6K phosphorylation was measured by intracellular staining in murine CD8+ T cells activated for 24 h with plate-bound anti-CD3 (coated at 5 µg / ml for 14 h at 4 °C in PBS; BioLegend, Cat. # 100331) and 1 µg / ml soluble anti-CD28 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and then surface labelled for 30 min at 4°C with TotalSeq-C0182 anti-mouse CD3 (clone 17A2, dilution 1:50, Biolegend, cat # 100263) and TotalSeq-C0002 anti-mouse CD8α (clone 53-6.7 ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated with the following antibodies for 30 min at 4°C: CD45.1-PerCP-Cy5.5 (BioLegend, Catalog No. 110728, 1/500), CD45.2-BV650 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated 72h at 4°C on a rotating wheel with the secondary antibody (1:500, Alexa-Fluor-555, 405324 P4U/BioLegend UK Ltd) diluted in 100% CAS-Block ...
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bioRxiv - Immunology 2023Quote: ... for 20 minutes at 4°C and stained in perm wash buffer with anti-IFNg PE or BV785 (ThermoFisher or BioLegend, clone: XMG1.2) for one hour at room temperature.
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with Fixable Viability Stain 510 for 15 minutes at 4 °C followed by anti-Fc blocking reagent (Biolegend, Cat# 101320) for 10 minutes prior to surface staining ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
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bioRxiv - Immunology 2023Quote: ... in FACS buffer for 30 minutes at 4°C prior to being stained with fluorescently conjugated antibodies to CD3e (Biolegend, clone 17A2), CD44 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Sections were stained using a three-colour protocol with 1:100 of each of the following antibodies in IF blocking buffer at 4 °C O/N: Alexa Fluor 488® anti-mouse GL-7 (GL-7; Biolegend, #144612), PE anti-mouse CD3 (145-2C11 ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS and stained at 4°C with 100 µL 1:500 Zombie Aqua viability dye (BioLegend, Cat# 423102) and 1:200 mouse FC block (TruStain FcX ...
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bioRxiv - Immunology 2023Quote: ... for 10 min at RT then stained for the following cell surface markers for 30 min at 4°C: CD3 (T cell marker, mouse anti-CD3-BV510, BioLegend, 1:50), CD14 (monocyte marker ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Immunology 2024Quote: ... Cells were harvested and stained for 30min at 4°C with the following antibodies: anti-CD25 BV785 (Biolegend, Cat: 356139, 1:50), anti-CD127 PE (Biolegend ...
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bioRxiv - Microbiology 2024Quote: ... and 100 ng/ml Escherichia coli lipopolysaccharides (LPS) for 24 hrs to induce M1 polarization or 25 ng/ml IL-4 (BioLegend catalog #766202) and 25 ng/ml IL-13 (BioLegend catalog #571102 ...
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bioRxiv - Immunology 2024Quote: Staining for surface markers of immune cells was carried out in the dark at 4°C for 15 – 30 minutes using the following antibody panel: BV510-CD45 (clone 30F11; Biolegend, CA, USA), PE-Cy7-TCRβ (clone H57-597 ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with antibody cocktail for surface markers at 4 °C for 30 min and then fixed using True nuclear transcription factor buffer set (Biolegend, Cat # 424401) for 20 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were stimulated as a single-cell suspension at a concentration of 4-5 x 106 cells/mL with the Cell Activation Cocktail (Biolegend, Cat # 423301) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... blotted membranes were blocked with 5% milk in TBST for 30 minutes and probed overnight (4°C) with primary antibody (anti-β-actin [Sigma-Aldrich, A5441], anti-HA [Biolegend, 901501]). After 3 washes with TBST ...
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bioRxiv - Biochemistry 2020Quote: ... Traditional antibodies based labeling procedure was done by using c-Myc Antibody (9E10, Cat # 626801, BioLegend, USA; incubation 1 h at 4°C) and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143 ...
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bioRxiv - Neuroscience 2020Quote: ... 0.05% Sodium Azide in PBS) and incubate overnight at 4°C with the monoclonal anti-amyloid beta antibody 6E10 (Biolegend, 803001, lot. B228658) at a concentration of 3μg/ml (Antibody buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Systems Biology 2019Quote: ... We washed the samples again with 0.1% BSA-PBS and then incubated for 10 minutes at 4°C with 1:250 anti-NGFR antibody conjugated directly to PE-Cy7 (Biolegend, 400126, clone ME20.4) in 0.5% BSA-PBS with 2mM EDTA ...
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bioRxiv - Immunology 2019Quote: ... Cell suspensions were incubated for 10 minutes at 4°C with 10μg/ml Fc Block (purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with anti-CD45-PE (1:300 ...
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bioRxiv - Immunology 2019Quote: Cells were incubated for 10 minutes at 4°C with 10μg/ml of purified rat anti-mouse CD16/CD32 (Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience ...