Labshake search

8901 - 8950 of 10000+ citations since 2019

• CARs are organized in nanodomains in the plasma membrane of T cells that accumulate at tumor contact sites

  Christina Verbruggen, et al., bioRxiv - Immunology 2023

  Quote: ... CAR-transduced (thus EGFRt+) T cells were detected by staining with anti-EGFR antibody (clone AY13, Biolegend, San Diego, CA, USA) and scFv-Linker (custom made ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... CD38-APC/Cy7 (BioLegend, 356615), human IgM-AF700 (BioLegend ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... CD27-APC (BioLegend, 356409), CD38-APC/Cy7 (BioLegend ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... human IgD-perCP/Cy5.5 (BioLegend, 348207), anti-His-FITC (Proteintech ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... anti-FLAG-PE (BioLegend, 637309) and DAPI ...
• Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including BQ and XBB lineages

  Ling Li, et al., bioRxiv - Immunology 2023

  Quote: ... human IgM-AF700 (BioLegend, 314537), human IgD-perCP/Cy5.5 (BioLegend ...
• Upregulated Fcrl5 disrupts B cell anergy and causes autoimmunity

  Chisato Ono, et al., bioRxiv - Immunology 2023

  Quote: ... TER-119 (TER-119) (all from BioLegend, BD Biosciences ...
• Upregulated Fcrl5 disrupts B cell anergy and causes autoimmunity

  Chisato Ono, et al., bioRxiv - Immunology 2023

  Quote: ... or MojoSort Mouse Pan B cell Isolation Kit (BioLegend), following the manufacturer’s instructions ...
• Dendritic Cells Overcome Cre/Lox Induced Gene Deficiency by Siphoning Material From Neighboring Cells Using Intracellular Monitoring—a Novel Mechanism of Antigen Acquisition

  Christopher H. Herbst, et al., bioRxiv - Immunology 2023

  Quote: ... Blocking antibodies specific for the following proteins were used: 2.5 μg/ml CD11b (M1/70, BioLegend, #101224), 2.5 μg/ml CD11c (N418 ...
• Dendritic Cells Overcome Cre/Lox Induced Gene Deficiency by Siphoning Material From Neighboring Cells Using Intracellular Monitoring—a Novel Mechanism of Antigen Acquisition

  Christopher H. Herbst, et al., bioRxiv - Immunology 2023

  Quote: ... A single cells suspension was generated and stained with eBioscience’s Fixable Viability Dye eFluor780 and anti-CD90.2 (30-H12, BioLegend, #105308) and anti-CD19 (6D5 ...
• IL-15 enhances anti-tumor immunity of sipuleucel-T by activating lymphocyte subsets and reversing immunoresistance

  Muhammad A. Saeed, et al., bioRxiv - Immunology 2023

  Quote: ... ELISA for IFN-γ and IL-10 were also performed with human ELISA MAX Deluxe Sets (BioLegend) to verify the multiplex ELISA results ...
• Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated T. gondii restriction in murine macrophages via vacuole nitration and intravacuolar network collapse

  Xiao-Yu Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... surface markers were stained with following antibodies at 1:200 dilution at 4°C for 30 min: CD11b- Pacific Blue (BioLegend, 101224), CD45-BV650 (BioLegend ...
• Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated T. gondii restriction in murine macrophages via vacuole nitration and intravacuolar network collapse

  Xiao-Yu Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... CD45-BV650 (BioLegend, 103151), CD11c-BV711 (BioLegend ...
• Inducible nitric oxide synthase (iNOS) is necessary for GBP-mediated T. gondii restriction in murine macrophages via vacuole nitration and intravacuolar network collapse

  Xiao-Yu Zhao, et al., bioRxiv - Immunology 2023

  Quote: ... I-A/I-E-AF647 ((BioLegend, 107618), Ly6G-APC/Cy7 (BioLegend ...
• Five inhibitory receptors display distinct vesicular distributions in T cells

  Jiahe Lu, et al., bioRxiv - Immunology 2023

  Quote: ... CL4 offspring were phenotyped by staining PBMCs with anti-CD8-APC (53-6.7, Biolegend, RRID: AB_312750) and anti-Vβ8.2 FITC (KJ16-133 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD200R3 (Ba13, BioLegend, 142206), anti-mCD24 (M1/69 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mFcεRIα (MAR-1, BioLegend, 134304), anti-mCD49b (HMα2 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mLy-6G (1A8, BioLegend, 127606 or 127633), anti-mGr-1 (RB6-8C5 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mLy-6C (HK1.4, BioLegend, 128037), anti-mLy-6G (1A8 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD49b (HMα2, BioLegend, 103503, or DX5, BD, 553856), anti-m/hCD11b (M1/70 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD80 (16-10A1, eBioscience, 12-0801-81, or BioLegend, 104710), anti-mCD86 (PO3.1 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD24 (M1/69, BioLegend, 101822), anti-mFcεRIα (MAR-1 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD45RB (16A, BD, 553100, or BioLegend, 103312), anti-mCD223 (C9B7W ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... anti-NK1.1 (1:200; BV510; BioLegend #108738), anti-F4/80 (1:100 ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... anti-TNF-α (1:100 dilution; clone MP6-XT22, BioLegend), IL-2 (1:100 dilution ...
• Integrated Organ Immunity: Antigen-specific CD4-T cell-derived IFN-γ induced by BCG imprints prolonged lung innate resistance against respiratory viruses

  Audrey Lee, et al., bioRxiv - Immunology 2023

  Quote: ... with additional anti-TCRg/d (1:50; PE; Biolegend #118108). Cells were then permeabilized using BD Fix/Perm for 20 min at RT and stained intracellularly with anti-IFN-γ (1:100 dilution ...
• Using CombiCells, a platform enabling titration and combinatorial display of cell surface ligands, to study T cell antigen sensitivity by TCRs, CARs, and BiTEs

  Ashna Patel, et al., bioRxiv - Immunology 2023

  Quote: Antibodies for Flow Cytometry (all from BioLegend): CD58 Clone ...
• TOM20 Gates PINK1 Activity And Mediates Its Tethering Of The TOM And TIM23 Translocases Upon Mitochondrial Stress

  Mohamed A. Eldeeb, et al., bioRxiv - Biochemistry 2023

  Quote: ... and exogenous HA (Biolegend, 902301), detected with donkey anti-mouse Alexa 488 (Thermo Fisher Scientific ...
• Eta-secretase-like processing of the amyloid precursor protein (APP) by RHBDL4

  Penalva Ylauna Christine Megane, et al., bioRxiv - Biochemistry 2023

  Quote: ... M3.2 (mouse Aβ region-specific, Biolegend), Y188 (APP C-terminus ...
• Eta-secretase-like processing of the amyloid precursor protein (APP) by RHBDL4

  Penalva Ylauna Christine Megane, et al., bioRxiv - Biochemistry 2023

  Quote: ... 1 mL of supernatant and 200 µl of lysate were incubated with 1.5 µg 6E10 antibody (Biolegend) in 500 µl PBS and 40 µl Protein G sepharose at 4°C rotating overnight ...
• Eta-secretase-like processing of the amyloid precursor protein (APP) by RHBDL4

  Penalva Ylauna Christine Megane, et al., bioRxiv - Biochemistry 2023

  Quote: ... immunoprecipitation was conducted for the supernantant as described above using 2 µg of M3.2 antibody (Biolegend) and 50 µl Protein G sepharose.
• Eta-secretase-like processing of the amyloid precursor protein (APP) by RHBDL4

  Penalva Ylauna Christine Megane, et al., bioRxiv - Biochemistry 2023

  Quote: ... 6E10 (human Aβ region-specific, Biolegend), 2E9 (epitope as determined by Willem et al. ...
• Multi-omics analyses identify transcription factor interplay in corneal epithelial fate determination and disease

  Jos GA Smits, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Primary antibody incubation was done overnight at 4οC with the following antibodies diluted in blocking buffer: PAX6 (1:300, 901301 Biolegend), P63 (1:100 ...
• Combination of NY-ESO-1-TCR-T-cells coengineered to secrete SiRPα decoys with anti-tumor antibodies to augment macrophage phagocytosis

  Evangelos Stefanidis, et al., bioRxiv - Bioengineering 2023

  Quote: ... followed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
• Solid tumor-on-chip model for efficacy and safety assessment of CAR-T cell therapy

  Tengku Ibrahim Maulana, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-human CD3 PE/Cyanine7 (317334; BioLegend), anti-human CD4 APC/Fire750 (357426 ...
• Solid tumor-on-chip model for efficacy and safety assessment of CAR-T cell therapy

  Tengku Ibrahim Maulana, et al., bioRxiv - Bioengineering 2023

  Quote: ... Purity of isolated T cell fractions was verified by staining with fluorophore-conjugated antibodies (all from Biolegend, San Diego, CA, USA) and 7-AAD staining solution (130-111-568 ...
• Beneficial metabolic effects of PAHSAs depend on the gut microbiota in diet-induced obese mice

  Jennifer Lee, et al., bioRxiv - Physiology 2023

  Quote: ... IFNγ (BV421; Biolegend Cat# 505829), TCRγδ (APC ...
• Oligodendrocyte maturation alters the cell death mechanisms that cause demyelination

  Timothy W. Chapman, et al., bioRxiv - Neuroscience 2023

  Quote: The following primary antibodies were used for immunofluorescence staining: mouse anti-CNPase (Biolegend, 1:500, Cat#836404), Guinea pig anti-CNPase (Synaptic Systems ...
• Immune Response of Transplanted Kidney Tissues Assembled from Organoid Building Blocks

  Thiago J. Borges, et al., bioRxiv - Bioengineering 2023

  Quote: ... HLA-DR-APC-Cy7 (1:50, clone L243, Biolegend), HLA-DR-BV650 (1:50 ...
• Immune Response of Transplanted Kidney Tissues Assembled from Organoid Building Blocks

  Thiago J. Borges, et al., bioRxiv - Bioengineering 2023

  Quote: ... CD123-BV711 (1:20, clone 6H6, Biolegend), CD27-BV650 (1:50 ...
• Immune Response of Transplanted Kidney Tissues Assembled from Organoid Building Blocks

  Thiago J. Borges, et al., bioRxiv - Bioengineering 2023

  Quote: ... We used the following anti-mouse antibodies: CD45-BV421 (1:100, clone 30-F11, Biolegend). After surface staining ...
• Injectable butyrate-prodrug micelles induce long-acting immune modulation and suppress autoimmune arthritis in mice

  Shijie Cao, et al., bioRxiv - Bioengineering 2023

  Quote: ... CD3 (BV605, Cat#100237, BioLegend), CD3 (BUV737 ...
• Injectable butyrate-prodrug micelles induce long-acting immune modulation and suppress autoimmune arthritis in mice

  Shijie Cao, et al., bioRxiv - Bioengineering 2023

  Quote: ... CD11b (BV711, Cat#101242, BioLegend), CD11c (BV421 ...
• Flow cytometric isolation of drug-like conformational antibodies specific for amyloid fibrils

  Alec A. Desai, et al., bioRxiv - Bioengineering 2023

  Quote: ... were incubated with either Tau-5 (Biolegend; 1:200) or AT8 (Invitrogen ...
• Engineered CD47 protects T cells for enhanced antitumor immunity

  Sean A. Yamada-Hunter, et al., bioRxiv - Bioengineering 2023

  Quote: ... NY-ESO-1 TCR was detected with antibodies specific for Vβ13.1 (BioLegend), the beta chain of the NY-ESO-1 TCR ...
• Proteomic profiling identifies Serpin B9 as mediator of resistance to CAR T-cell and bispecific antibody treatment in B-cell lymphoma

  Berit J. Brinkmann, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and viability dye e506 (#65-0866-18, all BioLegend). For quantification of activation markers ...
• MAT2A inhibition in AML unveils therapeutic potential of combining DNA demethylating agents with UPR targeting

  Keti Zeka, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Phenotyping of AML patient samples used a 1:50 dilution of the PE-Cy7-conjugated anti-human CD34 antibody (clone 581, Biolegend, UK). Samples were stained ...
• CD4⁺ T cell-derived IL21 regulates stem cell fate in acute myeloid leukemia by activation of p38-MAPK signaling

  Viviana Rubino, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Subsequently cells were washed with PBS and permeabilized with True-Phos™ Perm Buffer (BioLegend) according to the manufacturer’s protocol ...
• Kinetic and functional analysis of abundant microRNAs in extracellular vesicles from normal and stressed cultures of Chinese Hamster Ovary (CHO) cells

  Jessica Belliveau, et al., bioRxiv - Bioengineering 2023

  Quote: Viability was measured every other day using an Annexin V (BioLegend, San Diego, CA, USA) and 7AAD (BioLegend ...
• Solid tumor-on-chip model for efficacy and safety assessment of CAR-T cell therapy

  Tengku Ibrahim Maulana, et al., bioRxiv - Bioengineering 2023

  Quote: ... Zombie aqua dye (423102; BioLegend) was added 1:200 in the antibody-mixture solution to differentiate the viable cells during analysis ...