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Citations for BioLegend :
751 - 800 of 2106 citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... with 3 µg of APC-labeled anti-CD8b.2 antibody (clone 53-5.8, Biolegend) 5 min prior to euthanasia to mark vasculature-associated cells in vivo.
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bioRxiv - Immunology 2023Quote: ... Antibodies used for studies related to the Fra-2 model were purchased from Biolegend. Assessment of fibrosing alveolitis by Micro-CT images were obtained with a Perkin Elmer Quantum FX system (Caliper Life Sciences ...
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bioRxiv - Immunology 2023Quote: ... H-2Dd (Anti-mouse H-2Dd antibody; clone 34-2-12; Biolegend; Cat #110606), CD25 (BV510 Rat Anti-Mouse CD25 ...
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bioRxiv - Immunology 2024Quote: ... IFNg-PeCy7 (cat # 505826) and IL-2-PB (cat # 503820) were purchased from Biolegend. CD44-Percp-cyanine5.5 (cat# 45-0441-80 ...
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bioRxiv - Neuroscience 2023Quote: ... PE rat anti-mouse CD11b (cat # 101208, BioLegend, clone M1/70, 2 ng/μl), APC rat anti-mouse ACSA-2 (cat # 130-117-535 ...
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bioRxiv - Immunology 2023Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with fluorescently conjugated antibodies to CD3e (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... and secretion of IFNγ and IL-2 were measured by ELISA (ELISA MAX, BioLegend) in supernatant removed after 24-hour incubation.
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bioRxiv - Immunology 2023Quote: ... biotinylated SARS-CoV-2 Spike RBD protein was mixed with Streptavidin-BV421 (405225, BioLegend) at a 4:1 molar ratio for 1 hour at 4 °C to obtain the antigen probe ...
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bioRxiv - Cell Biology 2022Quote: ... Phycoerythrin-conjugated anti-human tetherin (BioLegend, RS38E, FC 2 μg / 100 μl / 106 cells), Pacific Blue-conjugated anti-CD71 (Exbio ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1-2 million cells were incubated in 45ul of Cell Staining Buffer (BioLegend) per million cells with Trustain FcX block (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... BV421 rat anti-mouse CD45 (cat # 103133, BioLegend, clone 30-F11, 2 ng/μl), PE rat anti-mouse CD11b (cat # 101208 ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were incubated with 4,6-diamidino-2-phenylindole (DAPI) and fluorophore-conjugated antibodies (BioLegend) against CD31 ...
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bioRxiv - Immunology 2023Quote: ... Cells were isolated from blood by adding 2 mL of RBC lysis buffer (Biolegend) per 100 uL of blood collected ...
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bioRxiv - Physiology 2024Quote: ... with 2% with Fetal Bovine serum with Fc block (Biolegend, San Diego, California, USA) and stained with monoclonal antibodies specific for CD66b (clone GM2H6) ...
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bioRxiv - Cancer Biology 2024Quote: Experiment 2: All conditions were multiplexed using Hashtag Oligonucleotide barcoding technology (TotalSeq-B, Biolegend) following manufacturer standard protocol ...
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bioRxiv - Immunology 2024Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with Zombie NIR (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... marrow cells were enriched for Kit+ progenitors using the Mojosort Mouse Lin-neg enrichment kit (BioLegend #480004). Blood was collected by cardiac puncture in EDTA-coated tubes and RBCs were lysed in two successive cycles of 5 mins each ...
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bioRxiv - Immunology 2024Quote: ... Marrow cells were enriched for Kit+ progenitors using the Mojosort Mouse Lin-neg enrichment kit (BioLegend #480004). GMPs were sorted into pre-chilled FBS-coated microcentrifuge tubes in 200 μl of PBS containing 0.5% BSA on the Beckman CytoFlex SRT sorter ...
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bioRxiv - Cancer Biology 2021Quote: 96-well round-bottom plates were coated with either 2.5 µg/mL anti-CD3 (Biolegend, clone 145-2C11,Cat# 100359) or HIgG (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... splenocytes from OT-II/Rag1/CD45.1 mice were cultured in a-CD28 coated plates (Biolegend, Cat. No. 102115, 1ug/ml) with complete RPMI (10%FBS ...
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bioRxiv - Immunology 2021Quote: ... Nunc ELISA plates were coated with 1 μg/ml Spike S1+S2ECD-His recombinant protein (SinoBiologicals) in ELISA coating buffer (Biolegend) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 1×105 TILs were seeded per well of a round bottom 96 well plate and stimulated with phorbol-12-myristate-13-acetate/Ionomycin in the presence of Brefeldin A (Biolegend) for 4 hours as per manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... The cells were activated in 96-well round bottom plates that were coated with 3ul/ml anti-CD3e (BioLegend #100202) and 5ul/ml anti-CD28 (BioLegend #102102 ...
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bioRxiv - Immunology 2019Quote: The following antibodies were used for coating of culture plates for in vitro T cell activation: anti-human CD3 (clone HIT3α; Biolegend), anti-human CD28 (clone CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... 0.05 mM β-mercaptoethanol) on a 96-well flat bottom plate coated with anti-CD3 antibody (10µg/ml, cat. # 317325, BioLegend). 7 x 103 DC3 or 5 x 104 monocytes were used per well ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed with water three times before applying 25 μL of secondary antibody (goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking solution ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were seeded at a density of 2.5 × 104/well in 96-well plates pre-coated with 1ug/ml CD3 (Biolegend100339) and 1ug/ml CD28 (Biolegend 102115) antibodies and cultured in 1640 media with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... cells were transferred to a non-CD3 coated plate and incubated for an additional three days before activation with Cell Activation cocktail with Brefeldin A (Biolegend) for 6 h and collection ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed four times with PBS-T and developed with 100 µL/well TMB High Sensitivity substrate solution (BioLegend, UK). After 5 to 10 min the reaction was stopped with 100 µL 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Immunology 2020Quote: ... 2 × 105 WT or CD56-KO NK92 and YTS cell lines were added to 12-well flat-bottomed plates that were pre-coated overnight at 4°C with anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2020Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2022Quote: ... On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend) with 10 µg/ml anti-CD200R1 (OX131 ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Cancer Biology 2020Quote: ... tumor cell lines were plated in triplicate on 96-well plates and treated for 72 hours with 10 ng/mL TNF (Biolegend) or 0.1% BSA vehicle control ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS and developed with 100 µl/well TMB High Sensitivity substrate solution (Biolegend). After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Immunology 2020Quote: ... total thymocytes were activated with 1 μg/mL plate bound anti-CD3 and soluble 1 μg/mL anti-CD28 in the presence of monensin (BioLegend). After 4hrs ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS and developed with 100 µl/well TMB high sensitivity substrate solution (Biolegend). After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transferred to a round-bottomed 96-well plate and were incubated with an AF647-conjugated anti-GFP antibody (1:20, BioLegend) and fixable viability dye eFluor 780 (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
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bioRxiv - Cell Biology 2024Quote: ... Suspensions were transferred to a round-bottomed 96-well plate and were resuspended in a cocktail consisting of an Alexa Fluor (AF) 647-conjugated anti-GFP antibody (BioLegend) and a eFluor 780 fixable viability dye diluted in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...
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bioRxiv - Immunology 2023Quote: ... the appropriate number of wells of a flat-bottomed tissue culture plate were coated with 10 μg/mL αCD3 (Biolegend) in PBS for 3h at 37°C ...
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bioRxiv - Physiology 2023Quote: Kupffer cells cultured in 96-well plates were supplemented with 30 μg EPO per well (recombinant rat EPO carrier-free, Biolegend). After 1,5 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfected cells were then stimulated for 24h at 37°C with plate bound 1μg/ml of anti-CD3 (clone UCHT1, BioLegend #300402) and 2μg/ml of anti-CD28 (clone CD28.2 ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs and HeLa target cells were co-cultured in u-bottom 96 well plates at a range of T:E ratios (indicated in figure legends) T cells were stimulated with 10 µg/mL plate-immobilised anti-CD3 (OKT3, BioLegend) and cultured for 4 days prior to staining with anti-CD8-APC (SK1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells of a 24-well plate were coated with 1 μg/mL anti-CD3 (#317326) and anti-CD28 antibodies (#302934, both BioLegend). For activation of T-cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11, BioLegend) and 1 μg mL−1 soluble anti-CD28 (eBioscience ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were activated in 96-wells culture plates (25 x103 cells/well) coated with anti-CD3 and anti-CD28 (Biolegend) or by the addition of the DPP-8/9 inhibitor Talabostat (Val-boroPro ...