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Citations for BioLegend :
651 - 700 of 1199 citations for Caspase 7 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Immunology 2019Quote: ... and stained with CD45.2-FITC (to identify leukocytes) and cell subset specific fluorochrome-conjugated mAb (all were obtained from BD Biosciences and Biolegend). Data were acquired on a BD LSR Fortessa flow cytometer with BD FACS Diva software and analyzed using FlowJo Software version 9.5 (Tree Star ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel anti-parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Immunology 2020Quote: ... The following surface antigens were detected by the indicated mAb clone: B220 (RA3-6B2-BV785 and RA3-6B2-BV421, BioLegend), CD21 (7EG-PerCP-Cy5.5 ...
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bioRxiv - Immunology 2021Quote: ... at 4°C overnight with 70 µl/well of 1 µg/ml of a relevant capture mAb: purified anti-human IFN-γ (Clone:NIB42, BioLegend); Purified anti-human TNF-α (Clone:MAb1 ...
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bioRxiv - Immunology 2022Quote: ... Intra-cellular labelling for detection of IL-10 was performed using the Intracellular Fixation & Permeabilization Buffer Set (eBioscience) and the PE mouse anti-human Anti-IL-10 mAb (Biolegend). Cells were kept at 4°C in 1.25% paraformaldehyde until analysis ...
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bioRxiv - Immunology 2024Quote: ... in the presence of S2 mAbs (5 μg/mL), protein transport inhibitor cocktail (1:500, eBioscience) and anti-CD107a APC (clone H4A3, BioLegend). Antibody-dependent NK cell activation was allowed to occur for 4 hours at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed and stained by incubation at 4 °C for 30 min with 40 µl of mAb anti-membrane marker mix containing CD4 APC/Cy7 (Biolegend), CD19 FITC ...
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bioRxiv - Immunology 2023Quote: Staining for flow cytometry was done using the following anti-mouse mAbs: CD3 (17A2) conjugated to PE/Dazzle 594 (BioLegend), CD4 (RM4-5 ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Immunology 2021Quote: ... 0.1% 2-mercapto-ethanol 1000X solution) supplemented with IL-7 (10 ng/mL, Biolegend). To activate T cells ...
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bioRxiv - Microbiology 2021Quote: ... cells were stained with Pacific Blue-Annexin V and 7AAD (7-aminoactinomycin D) (Biolegend) before running in an LSRII cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD15 antibody (phycoerithrin-cyanine 7 conjugated mouse anti-human, clone W6D3, BioLegend) to identify neutrophils ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was determined using flow cytometry and 7-AAD Viability Staining Solution (Biolegend). Cell counts for all fractions were ascertained using the Countess cell counter (Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... For dead cell exclusion a 7-AAD viability staining solution was employed (Biolegend, 420403) (5 µL per million cells) ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were resuspended in sorting buffer with 7-AAD (BioLegend, 420404; 1:100) to gate the viable cells ...
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bioRxiv - Cancer Biology 2024Quote: ... lymphoma cells were stained with 7-AAD Viability Staining Solution (cat. no. 420404, Biolegend) and Annexin V (cat ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in FACS buffer containing 0.5 µg/mL 7-AAD (Biolegend, 420403) and incubated on ice for 10 minutes and then acquired directly on the Attune NxT flow cytometer (Thermofisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.5 μL of 7-AAD and 2.5 μL of FITC-Annexin V (Biolegend #640922) were added ...
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bioRxiv - Microbiology 2023Quote: ... Dead cells (identified using 7-AAD or Zombie Fixable Viability Kits; both from BioLegend) and cell aggregates (identified on FSC-A versus FSC-H scatter plots ...
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bioRxiv - Immunology 2022Quote: ... FITC Annexin V Apoptosis Detection Kit with 7-AAD (#640922) was purchased from BioLegend.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined using flow cytometry and 7-AAD viability staining solution (Biolegend). Cell counts were ascertained using the Countess (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... biotin anti-CD3 (7 μg/mL) Abs for 1 h at room temperature (BioLegend, clones RA3-6B2 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.5 µL of 7-AAD and 2.5 µL of FITC-Annexin V (Biolegend #640922) were added ...
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bioRxiv - Immunology 2022Quote: ... then incubated at 37 °C in the presence or absence of recombinant murine CCL28 (BioLegend) at the indicated concentrations ...
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bioRxiv - Neuroscience 2020Quote: ... neuronal cultures were treated with 200 ng/mL recombinant human NRG1 (BioLegend, Cat. No. 551904) for 48 hrs ...
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bioRxiv - Immunology 2021Quote: ... Sorted transduced macrophages were stimulated with 5 ng/mL recombinant murine IFN-γ (575304, Biolegend) 12-14 hours before stimulation with 10 ng/mL recombinant TNFα as described above for 4 hours (FBS used in these experiments was from Omega ...
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bioRxiv - Immunology 2024Quote: ... BMDCs were generated by cultivating BM cells supplemented with recombinant mouse GM-CSF (#576308, BioLegend) or recombinant mouse Flt3L (550706 ...
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bioRxiv - Immunology 2024Quote: WT C57Bl/6 mice were injected intraperitoneally with 250 ng recombinant mouse IL-33 (Biolegend) in 100 µl PBS for three consecutive days ...
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bioRxiv - Developmental Biology 2023Quote: ... one uterine horn of XER mice was injected intraluminally with 1µg recombinant Lif (BioLegend, 554006) at 1300h on GD3 ...
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bioRxiv - Immunology 2023Quote: ... 550 µl of RPMI medium supplemented with 1 µg/ml recombinant human CCL21 (BioLegend; #582208) or nothing as negative control were added to the lower chambers ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng/ml recombinant mouse (rm)-SCF (Miltenyi) and 20 ng/ml rm-TPO (BioLegend), in the presence or absence of 300 pg/ml rm-IL21 (R&D Systems) ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng/ml recombinant mouse (rm)-SCF (Miltenyi) and 20 ng/ml rm-TPO (BioLegend), in the presence or absence of 300 pg/ml rm-IL21 (R&D Systems) ...
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bioRxiv - Immunology 2024Quote: ... The ultrasound-guided intra-amniotic injection of recombinant human HMGB1 (Biolegend, San Diego, CA, USA) at a concentration of 9 ng dissolved in 100 μL of sterile 1X phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2019Quote: ... 105 resting NK cells were mixed at 1:1 with 221 cell lines or peptide loaded target cells in presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). NK cells were then stained with mAbs to identify subsets based on the expression of KIR2DL1/S1 (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... and the secondary Alexa Fluor donkey 488-conjugated anti-mouse IgG pAb (Thermo Fischer Scientific, USA) and the Alexa Fluor 667-conjugated rat anti-mouse CD31 mAb (clone MEC133, Biolegend, USA).
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bioRxiv - Immunology 2019Quote: ... Antibody coated cells were mixed with resting NK cells in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Cells were then stained with mAbs to KIR2DS4 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... The purity of the sorted Treg subsets was assessed by staining the cells with FoxP3-APC mAb using the FoxP3 Fix/Perm Buffer Set for nuclear staining (Biolegend, USA). Over 95% of purity was detected in all cases analyzed ...
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bioRxiv - Immunology 2022Quote: ... Non-adherent cells were removed after 30min and adherent cells were incubated with αMHC DP-DQ-DR mAb (BioLegend, clone Tü39) or isotype control (IgG2a ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Immunology 2023Quote: ... centrifuged at 300 rpm for 3 mins followed by incubation at 37°C for 2hrs in the presence of BV421 conjugated anti-CD107a mAb (clone H4A3, Biolegend, #328626). Cell mixtures were then washed in PBS and stained with mAbs to CD56 (FITC or BV605 ...
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bioRxiv - Immunology 2024Quote: ... These cells were stained with a cocktail of mAb conjugated with DNA-barcoded oligonucleotide that are specific for immune cell lineages (TotalSeq™-C Human TBNK Cocktail, BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... stained with 1:200 mouse IgG1 KRT18-647 clone DA-7 (BioLegend, 628404, lot. B208126) for 30 min at RT ...
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bioRxiv - Immunology 2020Quote: ... while P14 T cells were stained with antibodies specific for Thy1.1 (clone OX-7, BioLegend), CD8 (clone 53-6.7 ...
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bioRxiv - Immunology 2021Quote: ... cells were stained using a FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend). Proliferation of cells was measured staining of control and Irradiated UVC with either 2 μM Cell Trace Yellow (Abcam ...
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bioRxiv - Immunology 2020Quote: ... as well as 7-AAD and Zombie Red viability dies were all purchased from BioLegend. AH1-loaded ...
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bioRxiv - Microbiology 2021Quote: ... cells were pre-incubated with 10 ug/mL anti-SIGLEC1 antibody (Biolegend, clone 7-239) for 30 min ...