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Citations for Roche :
1 - 50 of 2087 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from 200μL of VERO cells culture infected by herpes virus (treated or not with hemolymph) using the MagNA Pure extractor (Roche, Basel, Switzerland). The Herpes simplex virus quantitative real time PCR assay was performed was described by Read et al. ...
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bioRxiv - Microbiology 2023Quote: DNA was purified from virus-infected Vero cells stock by a commercially available kit (High Pure Extraction Kit; Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: Cytotoxicity of the identified drugs on Vero E6 cell was determined with WST-1 cell proliferation assays (ROCHE, 5015944001). Twenty thousand cells were seeded into a 96-well plate and incubated for 20–24 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... influenza virus hemagglutinin (Roche), followed by 1:4000 anti-rat horseradish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus was produced in 293T cells transfected using X-tremeGENE HP (Roche) with viral constructs along with VSV-G and gag-pol ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysate was bound to IgG-Sepharose beads (Roche) and washed by centrifugation with 100 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell lysate was first purified using Ni-NTA beads (Roche) and then subjected to size-exclusion chromatography on HiLoad Superdex 200 16/600 column (GE Healthcare ...
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bioRxiv - Zoology 2021Quote: ... was determined from the cell lysate using an ELISA kit (Roche) in accordance with the company instructions ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates were prepared using cOmplete™ Lysis-M (4719956001, Roche) with protease and phosphatase inhibitors and subjected to standard electrophoresis and transfer as previously described41 ...
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bioRxiv - Microbiology 2021Quote: ... or LightCycler Multiplex RNA Virus Master (Roche). SYBR green based RT-qPCRs were performed using Luna Universal One-Step RT-qPCR Kit (NEB).
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bioRxiv - Immunology 2021Quote: ... Cellular mRNA of cells not exposed to virus was isolated with an mRNA Capture kit (Roche) and cDNA was synthesized with a reverse-transcriptase kit (Promega) ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were prepared in lysis buffer supplemented with protease inhibitors (Roche) as described previously ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were prepared in RTK lysis buffer with protease (11836153001, Roche) and phosphatase (04906837001 ...
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bioRxiv - Immunology 2022Quote: Cell lysates were prepared in RIPA buffer containing protease inhibitor cocktail (Roche) and PMSF ...
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bioRxiv - Molecular Biology 2023Quote: ... cell lysates were prepared in the presence of cOmplete cocktail (11873580001, Roche) and 20 mM N-ethylmaleimide (NEM ...
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bioRxiv - Molecular Biology 2019Quote: ... avian myeloblastosis virus reverse transcriptase (AMV RT; Roche) were used to synthesize cDNA from total RNA (2.5 µg ...
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bioRxiv - Microbiology 2020Quote: ... or LightCycler® Multiplex RNA Virus Master (Roche) using CFX96 Real-Time System ...
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bioRxiv - Microbiology 2022Quote: ... or LightCycler® Multiplex RNA Virus Master (Roche) using the CFX96 Real-Time System ...
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bioRxiv - Cancer Biology 2021Quote: ... cell lysates were incubated further with protein G sepharose beads (Roche Applied Bioscience) for 2 hours at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Cell lysates were collected in RIPA buffer plus inhibitors (protease: complete mini Roche; 11836153001 ...
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bioRxiv - Immunology 2020Quote: ... β-galactosidase (lacZ) activity was measured in total cell lysates using CPRG (Roche) as a substrate ...
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bioRxiv - Developmental Biology 2021Quote: Virus-infected cells were lysed in RIPA buffer containing a protease inhibitor cocktail (cOmplete; #11697498001, Roche, IN, USA). Nuclear and cytoplasmic fractions of mHat9d cells were performed using the NE-PER Nuclear and Cytoplasmic Extraction reagents (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The Realtime Ready RNA Virus Master (Roche, Cat # 05619416001) was used to amplify viral RNA with the following primers/probe ...
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bioRxiv - Cell Biology 2021Quote: ... whole cell lysates were obtained by lysing the cells in RIPA buffer containing cOmplete Protease Inhibitor Cocktail (Roche). Following sonication ...
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bioRxiv - Cell Biology 2023Quote: Whole cell lysates were prepared via scraping cells in PBS containing protease inhibitors (Complete Protease inhibitor cocktail, Roche). Cells were pelleted and resuspended in hot SDS lysis buffer (0.5 % SDS ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from 200 µl of virus-containing cell culture supernatants using TriPure isolation reagent (Roche Applied Science) and purified according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Whole tissue or cell lysates were produced in RIPA buffer supplemented with PhosSTOP (Roche Diagnostics Corporation ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were generated using RIPA lysis buffer supplemented with protein inhibitor (Roche, #4693159001) and protein concentration was determined using Pierce™ BCA protein assay ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates (100–200 μg) were then immunoprecipitated with GFP antibody (Roche Applied Science). Ubiquitination was detected as described previously (38).
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bioRxiv - Molecular Biology 2021Quote: ... cell lysates were prepared using the IP lysis buffer (0.5% NP40 (Roche, Basel, Switzerland), 1x cOmplete protease inhibitor (Roche ...
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bioRxiv - Genetics 2020Quote: ... fly head or cell lysates were lysed in RIPA buffer with protease inhibitors (Roche) and passed through a 27-gauge needle to shear DNA ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates were prepared by lysing in RIPA buffer with protease inhibitor cocktail (Roche) and protein concentrations were normalized using a BCA assay (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were diluted with 500uL of RIPA buffer with protease inhibitor cocktail (Roche) and subjected to immunoprecipitation with G1 anti-DYKDDDDK affinity resin overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: Cell lysates were prepared in RIPA buffer supplemented with complete protease-inhibitor cocktail (Roche) and mixed in equal amounts with Laemmli buffer (Roth ...
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bioRxiv - Bioengineering 2023Quote: Cell lysate or conditioned medium were harvested and supplemented with protease inhibitor cocktail (Roche). After centrifugation at 14,000xg for 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... For IVD approved LightCycler® Multiplex RNA Virus Master (Roche) 5 μl of template RNA were used ...
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bioRxiv - Cancer Biology 2021Quote: Total cell lysates were made in RIPA-buffer supplemented with Protease inhibitor cocktail (11836170001, Roche), 1mM NaF and 1mM NaVO3 ...
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bioRxiv - Cancer Biology 2019Quote: Whole cell lysates were prepared in RIPA buffer with protease and phosphatase inhibitor cocktails (Roche). Lysates were subjected to SDS PAGE electrophoresis and transferred onto PDVF membrane (Millipore) ...
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bioRxiv - Systems Biology 2021Quote: Whole-cell lysates were obtained using RIPA buffer supplemented with protease and phosphatase inhibitors (Roche). An amount of 10 µg of protein was then resolved on 10-12.5% polyacrylamide gels and transferred to nitrocellulose membranes using the semi-dry technique ...
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bioRxiv - Microbiology 2022Quote: Whole-cell lysates were prepared using Triton-X sample buffer containing protease inhibitor cocktail (Roche). The protein concentration was assessed by using DC Protein assay reagent (Bio-Rad Laboratories) ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... The cell lysate was pre-cleaned with protein G agarose beads (Roche Diagnostic, Basel, Switzerland) for 1 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: Whole-cell lysates were prepared using Triton-X sample buffer containing protease inhibitor cocktail (Roche). The protein concentration was assessed by using DC Protein assay reagent (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2023Quote: ... C2C12 cell lysates were prepared in RIPA buffer containing protease inhibitors (Roche Diagnostics, Indianapolis, IN). To examine mitochondrial-associated proteins ...
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bioRxiv - Microbiology 2020Quote: Whole cell lysates were obtained by lysing cells in RIPA buffer supplemented with a cOmplete Mini protease inhibitor cocktail (Roche). Protein concentrations were measured using a BCA assay (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... For the IVD-approved LightCycler® Multiplex RNA Virus Master (Roche), 5 µL of template RNA in a total reaction volume of 20 µL was used ...
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bioRxiv - Cell Biology 2023Quote: ... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Virus passages were then amplified in penicillin-streptomycin-containing media (Roche) until cells became visibly swollen and demonstrated reduced viability statistics ...
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bioRxiv - Immunology 2021Quote: Splenocytes lysates were prepared using Cell Lysis Buffer (#9803, CellSignaling Technology) supplemented with protease inhibitors (Roche) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: Cell lysates in 1% sarcosyl were digested with 75 µg/ml proteinase K (PK; Roche, Switzerland) for 1 h at 37 °C and subsequently denatured in an equal volume of 2x sample loading buffer at 110 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (0.5 mg) were incubated with anti-HA antibody-coupled agarose beads (Roche, Basel, Switzerland) for 16 h at 4 °C ...