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Citations for Roche :
1 - 50 of 254 citations for Zika Virus Lysate DakArD 41662 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... influenza virus hemagglutinin (Roche), followed by 1:4000 anti-rat horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2021Quote: ... or LightCycler Multiplex RNA Virus Master (Roche). SYBR green based RT-qPCRs were performed using Luna Universal One-Step RT-qPCR Kit (NEB).
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bioRxiv - Molecular Biology 2019Quote: ... avian myeloblastosis virus reverse transcriptase (AMV RT; Roche) were used to synthesize cDNA from total RNA (2.5 µg ...
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bioRxiv - Microbiology 2020Quote: ... or LightCycler® Multiplex RNA Virus Master (Roche) using CFX96 Real-Time System ...
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bioRxiv - Microbiology 2022Quote: ... or LightCycler® Multiplex RNA Virus Master (Roche) using the CFX96 Real-Time System ...
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bioRxiv - Microbiology 2020Quote: ... The Realtime Ready RNA Virus Master (Roche, Cat # 05619416001) was used to amplify viral RNA with the following primers/probe ...
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bioRxiv - Microbiology 2020Quote: ... For IVD approved LightCycler® Multiplex RNA Virus Master (Roche) 5 μl of template RNA were used ...
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bioRxiv - Microbiology 2022Quote: ... For the IVD-approved LightCycler® Multiplex RNA Virus Master (Roche), 5 µL of template RNA in a total reaction volume of 20 µL was used ...
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bioRxiv - Cell Biology 2023Quote: ... with virus packaging and envelope expressing plasmids using Fugene-6 (Roche). Medium was replaced with DMEM with 10% FCS the next day and supernatant was harvested 3 days post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Virus passages were then amplified in penicillin-streptomycin-containing media (Roche) until cells became visibly swollen and demonstrated reduced viability statistics ...
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bioRxiv - Microbiology 2022Quote: ... using the LightCycler Multiplex DNA Virus Master kit (Roche Diagnostics, Mannheim, Germany) on a LightCycler 480 instrument (Roche Diagnostics).
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bioRxiv - Evolutionary Biology 2019Quote: Dengue virus was quantified via RT-qPCR using the LightCycler 480 (Roche). We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... including 5 μL template using LightCycler Multiplex RNA Virus Master (Roche, Switzerland). All assays were carried out in triplicate on the Roche Lightcycler 96 platform (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus was produced in 293T cells transfected using X-tremeGENE HP (Roche) with viral constructs along with VSV-G and gag-pol ...
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bioRxiv - Bioengineering 2021Quote: ... coli total tRNA from strain MRE600 was obtained from Roche Pharmaceuticals.
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bioRxiv - Biophysics 2019Quote: ... Lysates were incubated with Ni-NTA beads (Roche). After washing with the buffer containing 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2021Quote: ... The virus was pretreated with 20 U ml-1 RNase-free DNase I (Roche) for 1 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... DENV-3 RNA was amplified by qRT-PCR (LightCycler Multiplex RNA Virus Master, Roche), using primers to the conserved 3’UTR ...
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bioRxiv - Microbiology 2021Quote: RNA from virus was extracted using High Pure Viral Nucleic acid extraction kit (Roche). Using gene specific reverse primers of target genes ...
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bioRxiv - Microbiology 2022Quote: ... Virus RNA was purified using the High Pure Viral RNA kit (Roche, Basel, Switzerland), amplified using universal eight-segment primers and directly sequenced ...
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bioRxiv - Microbiology 2023Quote: ... DENV-2 RNA was amplified by qRT-PCR (LightCycler Multiplex RNA Virus Master, Roche), using primers to the conserved 3’UTR ...
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bioRxiv - Molecular Biology 2021Quote: ... falciparum W2 strain gDNA using Kapa 2G Robust DNA polymerase (Roche KK5024) with primers containing overhangs with the T7 promoter (forward primer ...
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bioRxiv - Genetics 2019Quote: ... coli strains using the High Pure plasmid isolation kit (Roche, Basel, Switzerland). PCR amplification of short DNA fragments (<3000 bp in length ...
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bioRxiv - Microbiology 2023Quote: ... and reverse transcripted using the Transcriptor First Strain cDNA Synthesis Kit (Roche).
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bioRxiv - Microbiology 2020Quote: Quantification of the virus by qPCR was performed using the LightCycler 480 System (Roche, France) and specific primers designed in the non-structural gene NS1 (qAalDV2-F ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysate was bound to IgG-Sepharose beads (Roche) and washed by centrifugation with 100 mM NaCl ...
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bioRxiv - Genetics 2020Quote: ... The lysate pretreated with RNase (RNase+ [10μg/ml, Roche]) was used as negative control (RNase+ control) ...
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bioRxiv - Cell Biology 2022Quote: ... the lysates were incubated with G-agarose beads (Roche) and rotated for 4 h at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Bacteria lysates were incubated with Ni+ resin (Roche cOmplete) for 2h at 4°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli total tRNA mixture (from strain MRE600) and phosphoenolpyruvate were purchased from Roche Applied Science ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli total tRNA mixture (from strain MRE600) and PEP was purchased from Roche Applied Science (Indianapolis ...
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bioRxiv - Molecular Biology 2023Quote: ... neoformans 150 TF mutant strains (46) was subjected to DNAse I treatment (Roche) to eliminate contaminating genomic DNA ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR was performed using the LightCycler Multiplex RNA Virus Master Kit (Roche Diagnostics, Mannheim, Germany) on a Cobas z480 analyzer (Roche Diagnostics ...
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bioRxiv - Immunology 2021Quote: ... Cellular mRNA of cells not exposed to virus was isolated with an mRNA Capture kit (Roche) and cDNA was synthesized with a reverse-transcriptase kit (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysate was incubated with cOmplete Ni-NTA resin (Roche) for 2h at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Lysates were treated with 600 U of RNase S7 (Roche) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were loaded onto His-tag purification column (Roche) and the protein was eluted in a buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was affinity purified by Ni-NTA beads (Roche) and the eluted protein was further purified by size-exclusion chromatography on HiLoad Superdex 200 16/60 column (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... DNA probes were generated for each strain by labeling with digoxigenin (Roche, Basel, Switzerland) 28 ...
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bioRxiv - Genomics 2019Quote: ... we used Escherichia coli k-12 strain MG1655 (hereafter E. coli; Roche, Basel, Switzerland), which has a high-quality genome sequence available (GenBank accession NC_000913 ...
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bioRxiv - Microbiology 2020Quote: ... All strains generated were verified by Southern blot analysis using digoxygenin labeled probes (Roche). For ip insertions ...
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bioRxiv - Microbiology 2022Quote: ... Avian influenza virus (AIV) RNA was subsequently extracted using the MagNA Pure 96 system (Roche, Basel, Switzerland). AIV was detected by a quantitative real-time RT-PCR targeting the matrix gene (M-PCR) ...
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bioRxiv - Developmental Biology 2021Quote: ... Whole brain lysate was precleared with Protein G agarose (Roche, 11243233001) and the supernatant was immunoprecipitated with CB1R antibody overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... The lysate was pre-cleared with protein G-agarose beads (Roche) and incubated with anti-FLAG M2 agarose beads (Sigma #A2220) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell lysate was first purified using Ni-NTA beads (Roche) and then subjected to size-exclusion chromatography on HiLoad Superdex 200 16/600 column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Lysate was resuspended in 2x protease inhibitor solution (cOmplete mini, Roche) and centrifuged for 10 min at 14,000 rpm and 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The lysates were pre-cleared with protein A-agarose beads (Roche) for 3 h and then incubated with anti-β1-integrin antibodies (MAB2000 from Millipore ...
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bioRxiv - Zoology 2021Quote: ... was determined from the cell lysate using an ELISA kit (Roche) in accordance with the company instructions ...
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bioRxiv - Cancer Biology 2021Quote: RLT Plus lysates with Complete Protease Inhibitor (Roche, catalog no. 11836170001) homogenized as described in the ‘RNA sequencing’ section were passed through the RNA binding column from the AllPrep DNA/RNA Mini kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates were prepared using cOmplete™ Lysis-M (4719956001, Roche) with protease and phosphatase inhibitors and subjected to standard electrophoresis and transfer as previously described41 ...