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Citations for Roche :
1 - 50 of 3786 citations for Water Nuclease Free Dist since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... EDTA-free (Sigma-Aldrich, Roche, 11836170001 in nuclease-free water), and added to 50-100 µL ribosome-coupled anti-Flag M2 agarose beads and 1 µL RNasin (Promega ...
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bioRxiv - Genetics 2021Quote: ... with addition of EDTA-free protease inhibitor (cOmplete tablet, freshly dissolved in nuclease free water to make a 100X stock, 5056489001, Roche) and lysed multiple times with resuspension every 2-3 mins during the 10mins incubation on ice ...
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bioRxiv - Neuroscience 2022Quote: ... the probe was diluted in nuclease free water and purified in a mini Quick-Spin column (Roche).
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bioRxiv - Microbiology 2020Quote: ... 4.8 μL Nuclease-free H2O (Roche), 0.4 μL primers (10 μM ...
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bioRxiv - Molecular Biology 2024Quote: ... primers and template in 10 μl of nuclease-free water were processed with LightCycler 480 Instrument II (Roche) as follows ...
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bioRxiv - Neuroscience 2024Quote: ... The template plasmid was diluted to 5×107 copies/µL with nuclease-free water plus yeast RNA (Roche, 10109223001), and this solution was further diluted to 2.5 ...
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bioRxiv - Cancer Biology 2021Quote: 1 μl sample from Denaturation was transferred to the qPCR plate well containing the qPCR Mix (Nuclease-free water + KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems) + cDNA Primers (from Visium Reagent kit)) ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was diluted 3-fold with nuclease-free water and 1.5 µL was PCR-amplified using 0.15 U KAPA HiFi HotStart DNA Polymerase (Kapa Biosystems, Wilmington, MA, USA) in a final volume of 10 µL ...
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bioRxiv - Biochemistry 2024Quote: ... nuclease (Pierce) and EDTA-free cOmpleteTM protease inhibitor cocktail (Roche). Cell debris were removed by centrifugation at 1000×g for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μL Turbo nuclease (Accelagen) and one tablet of Complete EDTA-free protease inhibitor (Roche) (Cormier et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 units of Micrococcal nuclease (Nuclease S7 Micrococcal Nuclease, cat# 10107921001; Roche Applied Science) with 200 units ExoIII (cat# M0206S ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μl of micrococcal nuclease (Nuclease S7, Roche) was added and the lysates were incubated at 25 °C for 18 min using a thermal cycler (Techne-Prime ...
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bioRxiv - Microbiology 2021Quote: ... with nuclease-free glycogen added to aid RNA precipitation (0.1 µg/µL final concentration, Roche diagnostics, Basel, Switzerland). DNA extractions used 0.14–0.89 g of crushed filter (1–47 extractions per sample) ...
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bioRxiv - Immunology 2020Quote: ... supplemented with BaseMuncher nuclease (Expedion; 1 ml per 40 ml cell suspension) and Complete EDTA-free protease inhibitor mix (Roche). The extract was precleared by centrifugation ...
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bioRxiv - Synthetic Biology 2021Quote: ... with 8 mL water plus one cOmplete™ Mini EDTA-free 11836170001 (Roche, Basel, Switzerland) protease inhibitor tablet) ...
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bioRxiv - Physiology 2022Quote: ... 2.5µl of cDNA in RNase free water was made up to 25µl with FastStart Universal SYBR Green Master (ROX, 12.5µl, Roche), Ultra Pure Water (8µl ...
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bioRxiv - Molecular Biology 2020Quote: MNase (Nuclease S7, Roche Cat: 10107921001) activity was tested at increasing KCl ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was then degraded into single nucleotides by overnight room temperature incubation with micrococcal nuclease (Nuclease S7, Roche) supplemented with 1 mM CaCl2 ...
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bioRxiv - Microbiology 2020Quote: ... 0,01% Benzonase® Nuclease (v/v) (Roche).
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bioRxiv - Microbiology 2022Quote: ... and nuclease S7 (0.25 U/μl, Roche) and then incubated 2 h at 37° C to eliminate cellular DNA/RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... 5U of MNase S7 micrococcal nuclease (Roche) were added ...
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bioRxiv - Physiology 2019Quote: ... 2.5 µl of cDNA in RNase free water was made up to 25 µl with FastStart Universal SYBR Green Master (ROX, 12.5 µl, Roche), Ultra Pure Water (8 µl ...
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bioRxiv - Pathology 2022Quote: ... 2.5 μl of cDNA in RNase free water was made up to 25 μl with FastStart Universal SYBR Green Master (ROX, 12.5 μl, Roche), Ultra Pure Water (8 μl ...
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bioRxiv - Developmental Biology 2022Quote: ... Precipitated RNA was resuspended in RNase- and DNase-free water and DNA digestion was performed using DNase I (Roche) with 2U/ul of RNasin® Plus (Promega ...
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bioRxiv - Plant Biology 2019Quote: ... 4 °C) the cleared supernatants were diluted 1 time using water containing 1x cOmplete ™ EDTA-free protease inhibitor (Roche). Protein extract was incubated overnight with anti-HA antibodies (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... washed in cold PBS and resuspended in 100 – 300 μl DI-water with 1mM PMSF and cOmplete EDTA-free protease inhibitor tablet (Roche). Resuspended pellets were freeze-thawed three times with liquid nitrogen/37°C water bath ...
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bioRxiv - Pathology 2022Quote: ... 2 μl of cDNA in RNase free water was made up to 20 μl with FastStart Universal SYBR Green Master (ROX, 10 μl, Roche), Ultra Pure Water (6.4 μl ...
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bioRxiv - Neuroscience 2023Quote: ... were pooled and diluted with RNase/DNase Free water to a final concentration of 0.2x and were combined with Fast Start Universal Master Mix (Roche, Basel, Switzerland) and cDNA for the preamplification PCR reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated with MNase (S7 Micrococcal nuclease, Roche) in the ratio 5 Units MNase/1 million cells at 37°C for 30-45 minutes and boiled in 1x Laemmli buffer (10% glycerol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We digested the total RNA by micrococcal nuclease (Roche) with 3U/ug of total RNA ...
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bioRxiv - Cell Biology 2022Quote: ... sm-nuclease and complete protease inhibitor cocktail by Roche) by sonication (Branson sonifier ...
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bioRxiv - Cell Biology 2022Quote: ... RRL was treated with S7 micrococcal nuclease (Roche, Germany) in the presence of CaCl2 to remove endogenous hemoglobin mRNA ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then quickly washed twice in wash+ buffer (20 mM HEPES pH7.5 + 150 mM NaCl + 0.1% BSA + 0.5 mM Spermidine + 1 large Roche complete EDTA-free tablets in water). Samples were resuspended in 150 μl Wash+C (wash+ + 100 mM CaCl2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Chromatin pellet was incubated with MNase (S7 Micrococcal nuclease, Roche) to ensure genomic DNA fragmentation ...
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bioRxiv - Developmental Biology 2022Quote: ... with micrococcal nuclease (MNase,15 units per tube; Roche, #10107921001) in an MNase buffer (0.3 M sucrose ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 µl of MNase (1 mg/ml; Nuclease S7, Roche) was added to the lysate ...
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bioRxiv - Biochemistry 2023Quote: ... Sm Nuclease and 1 tablet of Protease Inhibitor Cocktail (Roche)) followed by mechanical lysis with a Branson® sonifier ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 mM CaCl2 and 15 units of S7 micrococcal nuclease (Roche). Lysates were sonicated for 10 seconds at low power followed by incubation on ice for 30 minutes and clarification by centrifugation at 13,000 x g for 15 minutes at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated nuclei were then digested with MNase (S7 Micrococcal nuclease, Roche) and briefly sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was digested with 100 U/μg of S1 nuclease (Roche) at 37°C for 1 hour and was processed with the ChargeSwitch PCR Clean-up Kit (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... except that all digests contained 200 µg/mL fibrils and 40 µg/mL pronase E and that the digest was stopped in each 20 µL aliquot with 2 µL protease inhibitor cocktail solution (1 tablet cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, in 2 ml pure water) instead of PMSF solution ...
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bioRxiv - Biochemistry 2021Quote: ... One EDTA-free complete protease inhibitors EDTA free (Roche) was added in 100 mL lysis buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... dissolved in PCR grade water (Roche) at 3µg/µl and stored in aliquots at −80°C ...
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bioRxiv - Developmental Biology 2022Quote: ... and water) + Protease Inhibitors (Roche, USA) and quantified using Bradford reagents (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... EDTA-free (Roche)](modified from Buffer 2 ...
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bioRxiv - Molecular Biology 2019Quote: ... EDTA-free (Roche)] ...
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bioRxiv - Molecular Biology 2019Quote: ... EDTA-free (Roche). Lysates were clarified by centrifugation at 20,000 rcf for 60 min ...
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bioRxiv - Neuroscience 2022Quote: ... EDTA-free (Roche). The homogenate was centrifuged for 5min at 500 x g and the pelleted nuclei were resuspended in a nuclear storage buffer (15% sucrose ...
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bioRxiv - Biochemistry 2020Quote: ... EDTA-free (Roche) in nuclease-free water).
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CM1-driven assembly and activation of Yeast γ-Tubulin Small Complex underlies microtubule nucleationbioRxiv - Biochemistry 2021Quote: ... EDTA-free (Roche)) ...