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Citations for Roche :
1 - 50 of 1965 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Final libraries were analyzed by TapeStation electrophoresis and KAPA library quantification (Roche, Basel Switzerland, KK4835). The samples were pooled and sequenced on a NovaSeq instrument (Illumina ...
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bioRxiv - Biochemistry 2020Quote: ... Fractionated samples were analyzed by SDS-PAGE and electrophoresis for detection of HA-tagged Nedd4 (Roche anti-HA high affinity,1:2000 dilution ...
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bioRxiv - Zoology 2021Quote: ... All PCR products were checked through electrophoresis on 1.5% agarose gel and then purified using KAPA Pure Beads (Roche) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were boiled 5 min and 50 μg of proteins were separated by electrophoresis on 12.5% SDS-PAGE and subjected to immunoanalysis with either anti-GFP antibody (Roche), anti-Pgk1 antibody (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... separated by electrophoresis in a 0.8% agarose gel and transferred onto a nylon membrane (Hybond N+, Roche Molecular Biochemicals). The membrane was hybridized with digoxigenin-labeled DNA probes synthesized with a PCR DIG probe synthesis kit (Roche Molecular Biochemicals ...
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bioRxiv - Molecular Biology 2020Quote: ... The digested RNA samples were then analyzed by electrophoresis (5.5% PAGE with 8 M urea) and northern blotting using the DIG Northern Starter Kit (Roche). Poly(A ...
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bioRxiv - Developmental Biology 2021Quote: ... WB following 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) employed HRP-conjugated anti-HA antibody (Roche). Quantification was performed by Image J software.
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bioRxiv - Developmental Biology 2021Quote: ... WB following 4-20% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) employed HRP-conjugated anti-HA antibody (Roche). Quantification was performed by Image J software.
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bioRxiv - Cancer Biology 2023Quote: ... Whole cellular protein was extracted with RIPA buffer (Serva electrophoresis) containing protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche). Western blots were performed following routine protocols55 ...
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bioRxiv - Synthetic Biology 2019Quote: ... purified by gel electrophoresis and extracted from the gel via a commercial kit (HighPure PCR Product Purification Kit, Roche, DE). Sample preparation (Illumina TruSeq amplicon library ...
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bioRxiv - Developmental Biology 2021Quote: ... An equal amount of each protein sample was electrophoresed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (3010040001; Roche). The membrane was blocked with 5% non-fat dry milk for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified libraries were controlled by capillary electrophoresis (LabChip - Perkin) and quantitated by qPCR (KAPA Library Quantification Kits Illumina Platforms - Roche). Pooled libraries (12 pM ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were size fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred into a polyvinylidene difluoride membrane (Roche, Basel, Switzerland). Membranes were incubated (overnight ...
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bioRxiv - Cell Biology 2022Quote: ... in each group was separated by 10-12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride (PVDF) membrane (Roche, Basel, Switzerland). As for detection aggrecan ...
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bioRxiv - Cell Biology 2021Quote: ... Qualitative and quantitative analysis of the NGS library preparation process was conducted using a chip-based electrophoresis and libraries were quantified using a qPCR-based quantification kit (Roche, Cat. # KK4824). Sequencing was performed using Illumina NextSeq500 platform (Illumina Inc) ...
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bioRxiv - Neuroscience 2022Quote: ... 20 mg protein was loaded onto 8-10% SDS-PAGE gels for electrophoresis and subsequently transferred (90 V, 2-3 h) onto PVDF western blotting membranes (Roche, Cat. No. 3010040001) using the Criterion™ System BioRad ...
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bioRxiv - Cell Biology 2020Quote: Whole cells were lysed in 1X Cell Lysis Buffer Cell (Cell Signalling) supplemented with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). Protein concentration was determined by the Bradford assay using the Bradford reagent (Biorad ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed using 10X Cell Lysis Buffer (Cell Signalling) supplemented with protease and phosphatase inhibitors (Roche). Protein concentration was determined using a BCA protein assay kit (Pierce ...
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bioRxiv - Molecular Biology 2021Quote: ... cell viability was assessed using Cell Proliferation Kit I (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cell growth was monitored using a CASY cell counter (Roche). PCR products or plasmids linearized by NotI were transfected into cells by electroporation (Biorad) ...
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bioRxiv - Cell Biology 2024Quote: ... Cell growth was monitored using a CASY cell counter (Roche). Expression of GFP fusion proteins and RNAi were induced with doxycycline at a final concentration of 10 ng/mL and 1 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Cell growth was monitored using a CASY cell counter (Roche). PCR products or plasmids linearized by NotI were transfected into cells by electroporation (Biorad) ...
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bioRxiv - Systems Biology 2020Quote: ... Cell counting and assessment of cell viability were performed using the Cell Counter and Analyzer System (CASY, Roche).
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation in LNCaP IL1B overexpression and vector cells was assessed using Cell Proliferation Reagent WST-1 (Roche), per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Cells proliferation was assessed by BrdU Cell proliferation ELISA from Roche. IL-6 ELISA was from Affimetrics ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was evaluated using a colorimetric Cell Proliferation ELISA (Roche), based on the measurement of the incorporation of bromodeoxyuridine (BrdU ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell numbers were monitored using the CASY automated cell counter (Roche).
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bioRxiv - Biochemistry 2023Quote: Cell proliferation was determined using a colorimetric Cell Proliferation ELISA (Roche) kit ...
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bioRxiv - Immunology 2021Quote: Single-cells were sorted into PCR tubes containing 1μl of cell lysis solution (1:10 Cell Lysis buffer(Roche), 10U/μl Rnasin plus Ribonuclease inhibitor (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were harvested by centrifugation and red blood cells were removed using a red blood cell lysis buffer (Roche). Samples for intracellular staining were stained with zombie red for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: Viability and cell growth ratios were determined analyzing cell density by MTT using the Cell Proliferation Kit I (Roche, 11465007001) in DND41 cells treated with EX-527 (10-6 to 10-3 M ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then counted using a CASY cell counter (Roche, Basel, Switzerland). Final cell number was normalised to the initial cell count obtained at T0 ...
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bioRxiv - Cancer Biology 2022Quote: ... the cell pellet was resuspended in red blood cell lysis buffer (Roche) and incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... Red blood cells were lysed using red blood cell lysis buffer (Roche), after which cells were again washed twice with cold AdDF+++ ...
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bioRxiv - Biophysics 2020Quote: ... cell viability was determined using Cell Proliferation Reagent WST-1 (Roche, 5015944001) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Cell proliferation was assessed by Cell Proliferation ELISA BrdU kit (11647229001, Roche) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... before cell numbers were determined using a CASY TT Cell Counter (Roche). 4×106 cells were transferred to 96-well plates for antibody staining ...
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bioRxiv - Cancer Biology 2022Quote: ... B cells were thawed in B cell medium containing DNAse (Pulmozyme, Roche) 20 h before use in co-culture assays ...
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bioRxiv - Bioengineering 2023Quote: ... cell viability was measured by the MTT assay (Cell proliferation kit, Roche Diagnostics Deutschland GmbH ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability was assessed using the MTT cell proliferation kit I (Roche), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Whole protein lysates were isolated from iPS cells and iPS-ECs using the 1x Cell Lysis buffer (Cell Signalling, 9803) supplemented with protease inhibitors (Roche 11697498001). Cells were washed using PBS and harvested at the same passage and confluency (80% ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were centrifuged and cell killing assessed using the LDH Assay (Roche #11644793001) according to the manufacturers protocol using the SPECTROstar Nano (BMG Labtech).
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bioRxiv - Cell Biology 2022Quote: ... Cell pellet was resuspended in 2 mL Red blood cell lysis buffer (Roche) with 5 µL DNAse (1 mg/mL) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell pellets were resuspended in red blood cell lysis buffer (Roche, cat.# 11814389001) and incubated for 1 min at RT ...
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Senescence-Induced Immune Remodeling Facilitates Metastatic Adrenal Cancer in a Sex-Dimorphic MannerbioRxiv - Cancer Biology 2022Quote: ... Red blood cells were removed using Red Blood Cell Lysis buffer (Roche, 11814389001) according to manufacturer guidelines and the cells were washed twice in HBSS containing 2% FBS before counting on a Countess Automated Cell Counter (Thermo Fisher).
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bioRxiv - Immunology 2019Quote: ... Single-cell suspensions of lung cells were prepared by Liberase Blendzyme 3 (Roche) digestion of perfused lungs as previously described48 ...
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bioRxiv - Cancer Biology 2019Quote: ... The spleen cells were fused with P3U1 cells (ATCC) using PEG1500 (Roche Diagnostics,). The hybridomas were grown in RPMI 1640 medium (Nacalai Tesque ...
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bioRxiv - Biochemistry 2021Quote: ... cell viability was measured using the Cell Proliferation Kit I (Roche, Basel, Switzerland) in a 3-fold determination ...
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bioRxiv - Developmental Biology 2021Quote: ... To detect cell death the “In Situ Cell Death AP kit” (Roche, #11684809910) was used ...
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bioRxiv - Immunology 2020Quote: ... Cell numbers and viability were determined using a CASY TT Cell Counter (Roche). 2×106 cells were transferred to 96-well plates for antibody staining ...