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Citations for Roche :
1 - 50 of 65 citations for Tyr PDGF A Chain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Quantitative Polymerase Chain Reaction (qPCR) was performed on a LightCycler480 (Roche) using SYBR Green I master mix (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Quantitative polymerase chain reaction (qPCR) were performed in a LightCycler 480 (Roche) using the GoTaq® qPCR Master Mix kit (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... Polymerase Chain Reactions (PCRs) were performed with the Expand High Fidelity polymerase (Roche) and a TRIO-thermoblock (Biometra GmbH) ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using SYBR Green (Roche) on a LightCycler 480 (Roche) ...
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bioRxiv - Neuroscience 2021Quote: Real-Time polymerase chain reaction (RT-PCR) was performed using SYBR green (04887352001, Roche) on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Bioengineering 2022Quote: ... Polymerase chain reaction (PCR) was performed using KAPA2G Fast/HiFi HotStart ReadyMix kit (Kapa Biosystems). Plasmids and DNA were purified using Monarch kits (NEB ...
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bioRxiv - Microbiology 2021Quote: ... Polymerase chain reaction (PCR) was conducted using standard protocols for Taq polymerase (Roche, Mannheim, Germany). Default settings for PCR were an initial denaturation at 95 °C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Polymerase chain reactions (PCR) on bisulfite converted DNA was performed with FastStart Taq DNA Polymerase (Roche) in the following conditions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... real-time quantitative polymerase chain reaction (qPCR) (KAPA Library Quantification Kit; Kapa Biosystems, Wilmington, MA, USA), and small-scale sequencing (2 x146bp ...
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bioRxiv - Bioengineering 2021Quote: ... Quantitative Real-Time polymerase chain reaction (qRT-PCR; Light-Cycler 480 instrument Roche Diagnostics, Mannheim, Germany) analysis was performed for Collagen Type I (COL1A1) ...
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bioRxiv - Physiology 2021Quote: ... and quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using SYBR green (Roche; #04913914001). Quantification of messenger RNA (mRNA ...
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bioRxiv - Bioengineering 2021Quote: ... Quantitative polymerase chain reactions (qPCRs) were performed using LIGHTCYCLER 480 SYBR GREEN I Master Mix (Roche) either using 20 μL reaction volume in a LightCycler® 480 System II (Roche) ...
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bioRxiv - Immunology 2020Quote: ... quantitative polymerase chain reaction (RT-qPCR) was performed with SYBR FAST qPCR Master Mix (Kapa Biosystems) with primers shown in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2023Quote: Polymerase chain reaction: 240 μL of the PCR supermix (1.66x KAPA HiFi HotStart readymix (Roche, 07958935001), 1.33 μM PCR primer ...
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bioRxiv - Plant Biology 2024Quote: ... Polymerase chain reactions (PCRs) were done employing the KAPA HiFi HotStart ReadyMix PCR Kit (#KK2601; Roche Sequencing ...
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bioRxiv - Bioengineering 2020Quote: Quantitative Polymerase Chain Reactions (qPCR) were prepared in 10μ reactions with SYBR Green I Master Mix (Roche), 1μL of genomic extract as a template ...
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bioRxiv - Cell Biology 2019Quote: ... The COMMD1 sequence was amplified by polymerase chain reaction using the Pfu DNA polymerase (Roche, Basel, Switzerland), using an upstream primer to add a HindIII restriction site and retaining the SalI downstream of the coding sequence ...
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O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... The RBD fragment was amplified via polymerase chain reaction using Kapa HiFi HotStart ReadyMix (Roche, Basel, Switzerland) and then Gibson assembled in frame with an N-terminal His6-SUMO tag in the same vector as above ...
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bioRxiv - Immunology 2021Quote: ... Real-time quantitative polymerase chain reaction (qPCR) was performed using SYBR green PCR master mix (Kapa biosystems), forward and reverse primers (200 nM) ...
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bioRxiv - Microbiology 2023Quote: ... Polymerase Chain Reactions (PCR) were performed using KAPA HiFi HotStart Ready Mix® (KAPA biosystems, Boston, USA) with the following thermal parameters ...
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bioRxiv - Biochemistry 2024Quote: ... excluding mitochondrial targeting sequence) were amplified by polymerase chain reaction (PCR) using Kapa HiFi Hotstart ReadyMix (Roche) and inserted into a pET-SUMO expression vector containing an N-terminal hexahistidine-SUMO tag (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... equal amounts of heavy- and light chain pDNA were mixed with X-tremeGENE HP DNA Transfection Reagent (Roche) in FreeStyle™ 293 Expression Medium (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: Quantitative polymerase chain reaction (qPCR) was carried out using SYBR green mix buffer (Roche Applied Science, Meylan, France) in a LightCycler 480 Real-Time PCR System (Roche Applied Science ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) analysis was conducted on Roche Light cycler®96 (Roche Diagnostics, Mannheim, Germany) and amplification curve was checked with ROCHE software version 1.1 ...
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bioRxiv - Genetics 2022Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using KAPA SYBR FAST qPCR Master Mix (KM4101, KAPA Biosystems, USA). The 2-ΔΔCT method was used for quantitative analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... Humanization of the heavy (Vh) and light (Vl) chains of the best two candidates was performed by Roche using proprietary technology ...
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bioRxiv - Developmental Biology 2022Quote: ... Positive control embryos were incubated in polymerase chain reaction buffer containing 3 U/ml DNase I recombinant (Roche) for 1 h at 37°C before adding the TUNEL reaction mix ...
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bioRxiv - Immunology 2020Quote: ... and IgA) primers were used to amplify VH-region sequences using polymerase chain reaction (PCR high fidelity, Roche); separate PCR reactions for each VH-family were performed to avoid cross-priming or primer competition ...
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bioRxiv - Physiology 2022Quote: ... Quantitative real-time polymerase chain reaction (RT-PCR) was performed using SYBR Green protocols (Kapa Biosystems, MA, USA) on a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system) ...
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bioRxiv - Physiology 2023Quote: ... Real-time quantitative polymerase chain reaction (RT-qPCR) was carried out in a LightCycler 480 detection system (Roche) using the LightCycler FastStart DNA Master plus SYBR Green I kit (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... each DNA sample was amplified by polymerase chain reaction (PCR) using KAPA HiFi HS ReadyMix (KAPA Biosystems, Wilmington, MA) and primers specific for variable regions 3 and 4 of the 16S rRNA gene ...
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bioRxiv - Genomics 2019Quote: ... Gene expression was measured by quantitative Real Time - Polymerase Chain Reaction (qRT-PCR) (LightCycler 480, Roche Diagnostics, Basel, Switzerland) using the SYBR Premix Ex Taq (Tli RNaseH ...
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bioRxiv - Microbiology 2022Quote: ... Measurement of viral DNA was performed using a quantitative polymerase chain reaction (qPCR) assay (Cobas 6800; Roche, Basel, Switzerland).
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bioRxiv - Cancer Biology 2019Quote: Quantification of libraries was performed by quantitative polymerase chain reaction (qPCR) using the KAPA Library Quantification kit (KAPA Biosystems). Library fragment size was determined by the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Immunology 2020Quote: DNase treatment was performed prior to Reverse transcription polymerase chain reaction (RT-PCR) using RNAse-free DNase I (Roche) at 37°C for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... according to the manufacturer’s instructions and quantified by real-time quantitative polymerase chain reaction (RT-qPCR) performed on a LightCycler96 platform (Roche) using the iTaq Universal Probes One-Step RT-qPCR kit (BioRad ...
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bioRxiv - Physiology 2023Quote: ... and quantitative real-time polymerase chain reaction (RT-PCR) was performed with SYBR Green protocols (Kapa Biosystems, MA, USA) and a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system).29,30 Target gene expression was normalized to a housekeeping gene (ribosomal protein S18 ...
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bioRxiv - Microbiology 2020Quote: ... and via real-time quantitative polymerase chain reaction (qPCR) with the KAPA Library Quantification Kit (Kapa Biosystems, Wilmington, MA, USA). Final library pools were spiked with a non-indexed PhiX control library (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... Circular RNA levels were quantified by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), using Sybr Green mix (dssTakara, Japan) and detected by Lightcycler LC 480 (Roche). Primer sequences for qRT-PCR have been summarised in Supplementary Table 2.
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bioRxiv - Biochemistry 2022Quote: ... I analyzed samples by real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR) using a Light Cycler 1.5 (Roche Diagnostics). Measurements form RNA of vascular cell adhesion molecule-1 (VCAM-1 ...
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bioRxiv - Immunology 2020Quote: 100 ng TCRB chain cDNA was used as template for PCR with KAPA2G Fast Multiplex Mix (Kapa Biosystems, Wilmington, MA). Forward TCRB FR3 primers from the Full ...
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bioRxiv - Physiology 2021Quote: ... All mice were genotyped by polymerase chain reaction (PCR) using KAPA Taq PCR Kits (Kapa Biosystems, Inc., Wilmington, MA, USA). Specific primers purchased from Integrated DNA Technologies (Coralville ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using a FastStart Universal Probe Master (Rox) kit (Roche Applied Science) or Luna Universal Probe qPCR Master Mix (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... Reverse transcription polymerase chain reaction (RT–PCR) was performed on the LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland), and the cycling parameters were 1 cycle at 95 °C for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... The obtained library was amplified by emulsion polymerase chain reaction (PCR) using a GS FLK Titanium SV/LV emPCR Kit (Lib-L; Roche Diagnostics), added to a GS FLK Titanium PicoTiterPlate (Roche Diagnostics) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Polymerase chain reactions were carried out with a Roche LightCycler 480 system (384-well format, Roche Applied Science, Indianapolis, IN, USA) using a RT/HRM (real-time PCR/high-resolution melt ...
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bioRxiv - Cancer Biology 2021Quote: ... This was followed by 20 cycles of polymerase chain reaction (PCR) amplification using KAPA HiFi HotStart ReadyMix (#KK2602, Roche, Basel, Switzerland) and subsequent purification with Agencourt AMPure XP beads as described ...
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bioRxiv - Physiology 2021Quote: ... DNase treatment was performed on 300 ng RNA prior to Reverse Transcription Polymerase Chain Reaction (RT-PCR) using RNAse-free DNAse I (Roche, # 04716728001) at 37°C for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed with the KAPA SYBR Fast qPCR kit (KAPA Biosystems Cat# KK4608) on a CFX96 Real-Time PCR Detection System (Bio-Rad RRID ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real time polymerase chain reactions (qRT-PCR) were performed in 20 μL reactions using the FastStart universal SYBR Green master (Rox) kit (Roche diagnostics Corp.) on an Mx3000P real-time PCR machine (Agilent Technologies Inc. ...